Journal: Frontiers in Immunology

Article Title: Development of a prognostic model for hepatocellular carcinoma based on microvascular invasion characteristic genes by spatial transcriptomics sequencing

doi: 10.3389/fimmu.2025.1529569

Figure Lengend Snippet: Further validation of the model in TCGA, GEO, ICGC cohorts and HCC tissues. Survival curves, ROC curves and heatmaps of model gene expression for early HCC patients from (A) TCGA and (B) GSE14520 datasets. (C) Survival curves for HCC patients from TCGA, GSE14520, GSE76427, and LIRI-JP databases. (D) Violin plots of expression of prognostic model gene set in different regions in the spatial transcriptome. (E) The UAMP plot of prognostic model gene set in single-nucleus transcriptomics. (F) The expression of protective genes and risk genes in para-tumor and tumor tissue samples. (G) The expression of protective genes and risk genes in para-tumor, tumor and portal vein tumor thrombus tissue samples. (H) Immunoblotting of proteins in the prognostic model expression (CFL1, PIGO, GPX2, SLPI, CANX, DCN, CARHSP1) in para-tumor, tumor, and portal vein tumor thrombus tissues samples. L, para-tumor; T, tumor; P, pvtt. P < 0.05 is considered statistically significant. GEO, Gene Expression Omnibus; ICGC, International Cancer Genome Consortium. (* P < 0.05; ** P < 0.01; *** P < 0.001).

Article Snippet: Antibodies used are listed as follow: GAPDH (Proteintech, Cat No. 10494, 1:5000), SPLI (Abclonal, Cat No. A1897, 1:1000), GPX2(Abclonal, Cat No. A15999, 1:1000), CFL1 (Proteintech, Cat No. 10960, 1:3000), CANX (Proteintech, Cat No. 10427, 1:5000), DCN (Proteintech, Cat No. 14667, 1:2000), CARHSP1 (Proteintech, Cat No. 11672, 1:1500), PIGO (Abclonal, A18670, 1:1000).

Techniques: Biomarker Discovery, Gene Expression, Expressing, Western Blot