rock1 (Proteintech)
Structured Review
Rock1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rock1/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "ARHGEF10L Promotes Cervical Tumorigenesis via RhoA-Mediated Signaling"
Article Title: ARHGEF10L Promotes Cervical Tumorigenesis via RhoA-Mediated Signaling
Journal: Evidence-based Complementary and Alternative Medicine : eCAM
doi: 10.1155/2021/6683264
Figure Legend Snippet: Effects of ARHGEF10L overexpression on the RhoA pathway in HeLa cells. (a) Western blotting revealed the protein expression of GTP-RhoA in HeLa cells transfected with ARHGEF10L-expressing plasmids or blank vectors (Mock). (b) Western blotting revealed the protein expression of ROCK1 in HeLa cells transfected with the ARHGEF10L-expressing plasmid or the mock plasmid. (c) Western blotting revealed the protein expression of phospho-ezrin/radixin/moesin (pERM) in HeLa cells transfected with ARHGEF10L-expressing plasmids or mock. (d) Semiquantitation of the protein expression of GTP-RhoA, ROCK1, and pERM in HeLa cells. ∗ P < 0.05 and ∗∗ P < 0.01.
Techniques Used: Over Expression, Western Blot, Expressing, Transfection, Plasmid Preparation
rock1 polyclonal antibody (Proteintech)
Structured Review
Rock1 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rock1 polyclonal antibody/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rock1 (Proteintech)
Structured Review
Rock1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rock1/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Tissue Kallikrein Protects Rat Prostate against the Inflammatory Damage in a Chronic Autoimmune Prostatitis Model via Restoring Endothelial Function in a Bradykinin Receptor B2-Dependent Way"
Article Title: Tissue Kallikrein Protects Rat Prostate against the Inflammatory Damage in a Chronic Autoimmune Prostatitis Model via Restoring Endothelial Function in a Bradykinin Receptor B2-Dependent Way
Journal: Oxidative Medicine and Cellular Longevity
doi: 10.1155/2022/1247806
Figure Legend Snippet: Fibrosis manifestations of the rat prostate and the signal pathway of fibroblasts transdifferentiation into myofibroblasts. (a) Representative Masson photos of the prostate samples of the rats (upper, magnification ×100; lower, magnification ×200). (b) The positive area ratio of Masson staining. (c) Relative mRNA expression levels of COL1A1, COL3A1, α -SMA, and TGF- β to β -actin by QRT-PCR. (d) Representative western blot results of Collagen Type I, Collagen Type III, TGF- β , RhoA, and ROCK1 protein expressions. β -Actin served as an internal control. (e) The relative optical density quantification of the proteins mentioned above according to western blot. (f) The fluorescence intensity of α -SMA. (g) Representative IF photos of α -SMA of the prostate samples of the rats (upper, green, simple α -SMA staining, magnification ×200; lower, merge of α -SMA and DAPI, magnification ×200). Each bar in (b), (c), (e), and (f) represents the mean ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001.
Techniques Used: Staining, Expressing, Quantitative RT-PCR, Western Blot, Fluorescence
rock1 (Proteintech)
Structured Review
Rock1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rock1/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rock1 (Proteintech)
Structured Review
Rock1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rock1/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Lycopene Ameliorates Transplant Arteriosclerosis in Vascular Allograft Transplantation by Regulating the NO/cGMP Pathways and Rho-Associated Kinases Expression"
Article Title: Lycopene Ameliorates Transplant Arteriosclerosis in Vascular Allograft Transplantation by Regulating the NO/cGMP Pathways and Rho-Associated Kinases Expression
Journal: Oxidative Medicine and Cellular Longevity
doi: 10.1155/2016/3128280
Figure Legend Snippet: Expression of ROCK1 and ROCK2 in each group. ((a1)–(a3) and (b1)–(b3)) Immunohistochemical staining of ROCK1 and ROCK2 in grafted arteries (original magnification ×200). ((a4) and (b4)) The mean of the IOD/area of ROCK1 and ROCK2 in each group. IOD/area: integrated optical density/area; the mean of the IOD/area of positive staining was used to accurately measure the degree of positive expression in immunohistochemical staining. ∗ P < 0.01 for the isograft group compared with the allograft group; # P < 0.05 for the lycopene group compared with the allograft group. ((a5) and (b5)) Real-time quantitative PCR analysis of ROCK1 and ROCK2 mRNA expression in each group. ∗ P < 0.001 and # P < 0.05. ((a1) and (b1)) Isograft group; ((a2) and (b2)) allograft group; ((a3) and (b3)) lycopene group.
Techniques Used: Expressing, Immunohistochemical staining, Staining, Real-time Polymerase Chain Reaction
rock1 (Proteintech)
Structured Review
Rock1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rock1/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Reduced corporal fibrosis to protect erectile function by inhibiting the Rho-kinase/LIM-kinase/cofilin pathway in the aged transgenic rat harboring human tissue kallikrein 1"
Article Title: Reduced corporal fibrosis to protect erectile function by inhibiting the Rho-kinase/LIM-kinase/cofilin pathway in the aged transgenic rat harboring human tissue kallikrein 1
Journal: Asian Journal of Andrology
doi: 10.4103/1008-682X.189209
Figure Legend Snippet: Immunohistochemistry and Western blotting analysis of the activation of RhoA/ROCK pathway. Expressions of RhoA (a) and ROCK1 (b) were assessed through immunohistochemistry (×200). (c) Representative Western blotting results of RhoA, ROCK1, p-MYPT1 and β-actin in the corpus cavernosum of three groups. Expressions of RhoA (d), ROCK1 (e) and p-MYPT1 (f) with β-actin as the loading control in the corpus cavernosum of three groups were presented through bar graphs. Data are expressed as mean ± s.d. ( n = 10 rats per group). * P < 0.05 when comparing the two groups under each end of the capped line. yWTR: young wild-type Sprague-Dawley rats; aWTR: aged wild-type Sprague-Dawley rats; aTGR: aged transgenic rats; s.d.: standard deviation.
Techniques Used: Immunohistochemistry, Western Blot, Activation Assay, Transgenic Assay, Standard Deviation
rock1 (Proteintech)
Structured Review
Rock1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rock1/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Reduced corporal fibrosis to protect erectile function by inhibiting the Rho-kinase/LIM-kinase/cofilin pathway in the aged transgenic rat harboring human tissue kallikrein 1"
Article Title: Reduced corporal fibrosis to protect erectile function by inhibiting the Rho-kinase/LIM-kinase/cofilin pathway in the aged transgenic rat harboring human tissue kallikrein 1
Journal: Asian Journal of Andrology
doi: 10.4103/1008-682X.189209
Figure Legend Snippet: Immunohistochemistry and Western blotting analysis of the activation of RhoA/ROCK pathway. Expressions of RhoA (a) and ROCK1 (b) were assessed through immunohistochemistry (×200). (c) Representative Western blotting results of RhoA, ROCK1, p-MYPT1 and β-actin in the corpus cavernosum of three groups. Expressions of RhoA (d), ROCK1 (e) and p-MYPT1 (f) with β-actin as the loading control in the corpus cavernosum of three groups were presented through bar graphs. Data are expressed as mean ± s.d. ( n = 10 rats per group). * P < 0.05 when comparing the two groups under each end of the capped line. yWTR: young wild-type Sprague-Dawley rats; aWTR: aged wild-type Sprague-Dawley rats; aTGR: aged transgenic rats; s.d.: standard deviation.
Techniques Used: Immunohistochemistry, Western Blot, Activation Assay, Transgenic Assay, Standard Deviation
protein kinase 1 (Proteintech)
Structured Review
Protein Kinase 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/protein kinase 1/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
rock1 (Proteintech)
Structured Review
Rock1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rock1/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Tetrandrine Attenuates Podocyte Injury by Inhibiting TRPC6-Mediated RhoA/ROCK1 Pathway"
Article Title: Tetrandrine Attenuates Podocyte Injury by Inhibiting TRPC6-Mediated RhoA/ROCK1 Pathway
Journal: Analytical Cellular Pathology (Amsterdam)
doi: 10.1155/2022/7534181
Figure Legend Snippet: Tetrandrine (Tet) inhibits TRPC6 expression and RhoA/ROCK1 pathway in vivo . (a) The mRNA expression of TRPC6, synaptopodin, RhoA, and ROCK1 in kidney tissues of rats was measured by RT-qPCR. (b) The protein expression of TRPC6, synaptopodin, RhoA, and ROCK1 in kidney tissues of rats was detected by western blotting. Nephropathy rats were induced by adriamycin (ADRIA) and then treated with low-dose (LD), medium-dose (MD), and high-dose (HD) Tet, or cyclosporine A (CsA, a positive drug). ∗ P < 0.05 and ∗∗ P < 0.01 versus the control group. # P < 0.05 and ## P < 0.01 versus the ADRIA group.
Techniques Used: Expressing, In Vivo, Quantitative RT-PCR, Western Blot
Figure Legend Snippet: Tetrandrine (Tet) supresses the TRPC6-mediated RhoA/ROCK1 pathway in podocytes. (a) The mRNA expression of TRPC6, synaptopodin, RhoA, and ROCK1 in MPC5 podocytes was measured by RT-qPCR. (b) The protein expression of TRPC6, synaptopodin, RhoA, and ROCK1 in MPC5 podocytes was determined by western blotting. MPC5 podocytes were transfected with lev-NC or lev-TRPC6 and then treated with U73122 (an inhibitor of TRPC6 channel opening), CsA, or Tet. ∗∗ P < 0.01 versus the lev-NC group. # P < 0.05 and ## P < 0.01 versus the lev-TRPC6 group.
Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Transfection
21850 1 ap (Proteintech)
Structured Review
21850 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/21850 1 ap/product/Proteintech
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99