Novus Biologicals
peroxidase conjugated goat anti human plasminogen Peroxidase Conjugated Goat Anti Human Plasminogen, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 82/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/peroxidase conjugated goat anti human plasminogen/product/Novus Biologicals Average 82 stars, based on 1 article reviews
peroxidase conjugated goat anti human plasminogen - by Bioz Stars,
2025-02
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Proteintech
anti nrbp1 2 antibody ![]() Anti Nrbp1 2 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/anti nrbp1 2 antibody/product/Proteintech Average 93 stars, based on 1 article reviews
anti nrbp1 2 antibody - by Bioz Stars,
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Proteintech
nrbp2 ![]() Nrbp2, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/nrbp2/product/Proteintech Average 94 stars, based on 1 article reviews
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Proteintech
antibody rabbit anti nrbp2 ![]() Antibody Rabbit Anti Nrbp2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/antibody rabbit anti nrbp2/product/Proteintech Average 93 stars, based on 1 article reviews
antibody rabbit anti nrbp2 - by Bioz Stars,
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Journal: bioRxiv
Article Title: Pseudokinase HPO-11 inhibits nonsense-mediated decay to ensure genome stability in C. elegans
doi: 10.1101/2022.09.04.506508
Figure Lengend Snippet: FLAG-HPO-11 interactome a. The difference of log 2 -transformed mean LFQ intensities of FLAG-HPO-11 IP and control IP was plotted against the negative log 10 p-value of a one-sided two-sample t-test (n=3). Proteins with a p-value below 0.05 and a student’s t-test difference of at least 2 were considered as significantly enriched candidates (light grey). The bait protein HPO-11 is shown in green. Homologs of known NRBP1 interactors (ELB-1, ELC-1 and Y48C3A.12) are depicted in dark grey, SMG-2 in orange. b. Gene ontology term enrichment analysis for the interactors of HPO-11. Selected enriched GO terms are shown with significance.
Article Snippet: Anti-Flag (F3165, Sigma-Aldrich), anti-V5-Tag antibody (ab27671, Abcam), anti-GFP antibody (ab290, Abcam), Anti-DNA-RNA hybrid antibody S9.6 (MABE1095, Sigma-Aldrich),
Techniques: Transformation Assay
Journal: bioRxiv
Article Title: Pseudokinase HPO-11 inhibits nonsense-mediated decay to ensure genome stability in C. elegans
doi: 10.1101/2022.09.04.506508
Figure Lengend Snippet: h p o -11 and its human homologs NRBP1 and NRBP2 share conserved functions a. NRBP1/2 interact with UPF1 in MCF-7 cells. Shown is one representative result of three biological replicates. b. siRNA knock-down of either NRBP1 or NRBP2 caused DNA damage response. Shown is a western blot of γ-H2AX in HEK293T cell, N = 3 biological replicates.
Article Snippet: Anti-Flag (F3165, Sigma-Aldrich), anti-V5-Tag antibody (ab27671, Abcam), anti-GFP antibody (ab290, Abcam), Anti-DNA-RNA hybrid antibody S9.6 (MABE1095, Sigma-Aldrich),
Techniques: Western Blot
Journal: Cancers
Article Title: Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression
doi: 10.3390/cancers12061483
Figure Lengend Snippet: Expression of nuclear receptor binding protein 2 (NRBP2) in human brain tumors. ( A ) Quantification of the NRBP2 positive staining area in human brain tumors; ( B ) Quantification of NRBP2 staining intensity in human brain tumors; ( C ) Bubble plot comparing the staining quantity (% positive staining) and intensity of NRBP2 in the nucleus (left) or cytoplasm (right) across all samples; ( D ) Immunohistochemistry staining of NRBP2 in non-tumor brain and medulloblastoma tissues of the same tissue microarray, scale bar 20 µm; ( E ) Top panel: NRBP2 protein expression (western blot) in fetal human cerebellum and in medulloblastoma cells D283, D324, MB002 and sD425, Bottom panel: Relative NRBP2 protein expression, normalized to b-actin as loading control of the western blot above, setting fetal human cerebellum (CB) = 1; ( F ) NRBP2 gene expression levels (GSE107405) in MB cell lines (D283, D324, MB002, sD425) in triplicates, and single samples of human cerebellar astrocytes (HA-c) and human spinal cord astrocytes (HA-sp); ( G ) NRBP2 is mainly present in the cytoplasm as seen by subcellular fractionation followed by western blot of cell lysates from a central nervous system(CNS) embryonal tumor cell line PFSK, MB cell lines D283 and D324, and glioblastoma cells U3013MG (Left panel) and the corresponding quantification of intensity (Right panel).
Article Snippet: The membrane was blocked in 5% non-fat milk in TBST (Tris buffered saline supplemented with Tween-20)for 1 h at room temperature and then incubated with primary
Techniques: Expressing, Binding Assay, Staining, Immunohistochemistry, Microarray, Western Blot, Fractionation
Journal: Cancers
Article Title: Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression
doi: 10.3390/cancers12061483
Figure Lengend Snippet: Expression of nuclear receptor binding protein (NRBP2) in human medulloblastoma. ( A ) NRBP2 gene expression levels (GSE124814) in normal human cerebellum, either for pediatric cases only (age < 18 years; n = 47) or across pediatric and adult cases (all ages; n = 291), and MB patients ( n = 1350); ( B ) NRBP2 gene expression levels (GSE85217) in wingless (WNT) ( n = 70), sonic hedgehog (SHH) ( n = 223), Group 3 ( n = 144), and Group 4 ( n = 326) subgroups of medulloblastoma patients. Boxes indicate the range between the first and third quartiles, black horizontal lines represent the median value, and whiskers extend to the extreme values excluding outliers, which are shown as dots; ( C ) NRBP2 protein expression levels (MSV00008264) in WNT ( n = 3), SHH ( n = 15), Group 3 ( n = 14) and Group 4 ( n = 13) subgroups of MB patients. Boxes indicate the range between 1–99 percentile, black horizontal lines represent the median value, and whiskers extend to the extreme values. Unpaired t test between the SHH and G3 groups showed a significant difference (* denotes significance as p < 0.05).
Article Snippet: The membrane was blocked in 5% non-fat milk in TBST (Tris buffered saline supplemented with Tween-20)for 1 h at room temperature and then incubated with primary
Techniques: Expressing, Binding Assay
Journal: Cancers
Article Title: Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression
doi: 10.3390/cancers12061483
Figure Lengend Snippet: Effects on nuclear receptor binding protein (NRBP2) mRNA expression and cell growth of medulloblastoma (MB) cell lines by promotor de-methylation and histone de-acetylation treatment. ( A ) relative NRBP2 expression in D283, D324, sD425, and MB002 cell lines treated with 5-Aza-2′-deoxycytidine (dAC) for two days, expressed as RQ value, 2-(ΔΔCt) value, beta-actin was used as an endogenous control and untreated cells as the reference. n = 3; ( B ) relative NRBP2 expression in D283, D324, sD425, and MB002 cell lines treated with Valproic acid (VPA) for two days, expressed as RQ (relative quantification) value, 2-(ΔΔCt) value, beta-actin was used as an endogenous control and untreated cells as the reference. n = 3; ( C ) Left Upper panel: NRBP2 protein expression in MB002, sD425, and D324 cell lines treated with dAC for two days. Left Lower panel: Signal intensity quantification by ImageJ. Right Upper panel: NRBP2 protein expression in MB002, sD425 and D324 cell lines treated with VPA for two days. Right Lower panel: Signal intensity quantification by ImageJ (an open source image processing program developed at the National Institute of Health, USA) . b-actin was used as a loading control for both; ( D ) Left panel: growth curves for MB002 (upper) and sD425 (lower) cell lines treated with VPA compared to untreated cells. Right panel: growth curves for MB002 (upper) and sD425 (lower) cell lines treated with dAC compared to untreated cells. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ( E ) Left panel: relative gene expression levels of NCOR after transfection by off-target-siRNA, NCOR-siRNA, SMRT-siRNA, and NCOR and SMRT-siRNA; middle panel: relative gene expression levels of SMRT after treatment by off-target-siRNA, NCOR-siRNA, SMRT-siRNA, and NCOR and SMRT-siRNA; right panel: relative gene expression levels of NRBP2 after treatment by off-target-siRNA, NCOR-siRNA, SMRT-siRNA, and NCOR and SMRT-siRNA. n = 3. Average expression levels after off-target-siRNA transfection were used as control.
Article Snippet: The membrane was blocked in 5% non-fat milk in TBST (Tris buffered saline supplemented with Tween-20)for 1 h at room temperature and then incubated with primary
Techniques: Binding Assay, Expressing, Methylation, Transfection
Journal: Cancers
Article Title: Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression
doi: 10.3390/cancers12061483
Figure Lengend Snippet: Overexpression of nuclear receptor binding protein (NRBP2) in D324 medulloblastoma (MB) cells causes reduction of cell numbers, migration, and invasion. ( A ) Western blot of NRBP2 in D324 cells transfected with an empty vector (cntl) or NRBP2-IRES2-eGFP NRBP2 sequence with V5 tag and linked with the internal ribosome entry site (IRES2) and the enhanced green fluorescent protein (eGFP) coding region) overexpression plasmid, Left panel: Signal intensity quantification by ImageJ; ( B ) growth rate of D324 cells transfected with control vector or NRBP2 vector; ( C ) Western blot of NRBP2 in D324 transduced with scrambled shRNA, or shRNA against NRBP2. The cultures were transduced twice to increase the efficiency of downregulation, Left panel: Signal intensity quantification by ImageJ; ( D ) growth curve of D324 cells transduced with scrambled shRNA, or shRNA against NRBP2; ( E ) photomicrographs of cell migration after scratching the cell monolayer ( n = 3); ( F ) quantification of coverage of cell-free area from ( C ) ( n = 3); ( G ) photomicrographs of cell invasion in collagen gel at different time points of D324 cells transduced with scrambled shRNA, or shRNA against NRBP2; ( H ) quantification of invasion from ( E ) ( n = 3). * p < 0.05, ** p < 0.01, **** p < 0.0001.
Article Snippet: The membrane was blocked in 5% non-fat milk in TBST (Tris buffered saline supplemented with Tween-20)for 1 h at room temperature and then incubated with primary
Techniques: Over Expression, Binding Assay, Migration, Western Blot, Transfection, Plasmid Preparation, Sequencing, Transduction, shRNA
Journal: Cancers
Article Title: Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression
doi: 10.3390/cancers12061483
Figure Lengend Snippet: Overexpression of nuclear receptor binding protein (NRBP2) in medulloblastoma (MB) leads to increased cell death. ( A ) flow-cytometry-based quantification of live and dead D324 cells 1 and 2 days post-transfection with NRBP2 -V5-IRES-eGFP (NRBP2 sequence with V5 tag, linked with the internal ribosome entry site (IRES2) and the enhanced green fluorescent protein (eGFP) coding region) plasmid; ( B ) flow-cytometry-based cell-cycle analysis in D324 cells after transfection with NRBP2 -V5-IRES-eGFP plasmid; ( C ) Western blot of Ki67 in MB002 and sD425 cells 2–24 h post transduction with empty vector or EF1A-NRBP2(V5)-plasmid. Corresponding Signal intensity quantification by ImageJ on the left; ( D ) Western blot of phosphorylation of AKT in D324 cells transfected with control or NRBP2 plasmid and signal intensity quantification by ImageJ on the left; ( E ) apoptosis analysis based on Annexin V labelling of control-transfected or NRBP2-transfected D324 cells; ( F ) Western blot of cleaved caspase-3 in MB002 and sD425 cells transduced with empty vector or NRBP2 overexpressing plasmid, 48 h post transduction; ( G ) cytospin preparations of MB002 cells, 4 to 48 h after transduction with a EF1A-NRBP2(V5)- plasmid showing staining for NRBP2 (upper panel) and cleaved caspase-3 (lower panel); ( H ) expression of apoptotic genes BAK1 and BAX mRNA in D324 cells transfected with control or NRBP2 plasmid; ( I ) Western blot of BAK1 and BAX in MB002 and sD425 cells transduced with control or NRBP2 plasmid 48 h post transduction. Corresponding signal intensity quantification by ImageJ is shown on the left.
Article Snippet: The membrane was blocked in 5% non-fat milk in TBST (Tris buffered saline supplemented with Tween-20)for 1 h at room temperature and then incubated with primary
Techniques: Over Expression, Binding Assay, Flow Cytometry, Transfection, Sequencing, Plasmid Preparation, Cell Cycle Assay, Western Blot, Transduction, Staining, Expressing
Journal: Cancers
Article Title: Nuclear Receptor Binding Protein 2 Is Downregulated in Medulloblastoma, and Reduces Tumor Cell Survival upon Overexpression
doi: 10.3390/cancers12061483
Figure Lengend Snippet: List of sequences of the primers used for qPCR.
Article Snippet: The membrane was blocked in 5% non-fat milk in TBST (Tris buffered saline supplemented with Tween-20)for 1 h at room temperature and then incubated with primary
Techniques: