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<t>C-FOS</t> expression of hypothalamic nuclei. ( A ) Arc nuclei (×200). ( B ) VMN nuclei (×200). ( C ) DM nuclei (×200). ( D ) PV nuclei (×200). Scale bar=100 μm. ( E ) The 2 ends of the horizontal line indicate a comparison between the 2 groups. a–d is a bar chart comparing the C-FOS expression levels of the hypothalamic A–D nucleus (* P <0.05, ** P <0.01, **** P <0.001, ns: no significance).
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<t>C-FOS</t> expression of hypothalamic nuclei. ( A ) Arc nuclei (×200). ( B ) VMN nuclei (×200). ( C ) DM nuclei (×200). ( D ) PV nuclei (×200). Scale bar=100 μm. ( E ) The 2 ends of the horizontal line indicate a comparison between the 2 groups. a–d is a bar chart comparing the C-FOS expression levels of the hypothalamic A–D nucleus (* P <0.05, ** P <0.01, **** P <0.001, ns: no significance).
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<t>C-FOS</t> expression of hypothalamic nuclei. ( A ) Arc nuclei (×200). ( B ) VMN nuclei (×200). ( C ) DM nuclei (×200). ( D ) PV nuclei (×200). Scale bar=100 μm. ( E ) The 2 ends of the horizontal line indicate a comparison between the 2 groups. a–d is a bar chart comparing the C-FOS expression levels of the hypothalamic A–D nucleus (* P <0.05, ** P <0.01, **** P <0.001, ns: no significance).
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<t>C-FOS</t> expression of hypothalamic nuclei. ( A ) Arc nuclei (×200). ( B ) VMN nuclei (×200). ( C ) DM nuclei (×200). ( D ) PV nuclei (×200). Scale bar=100 μm. ( E ) The 2 ends of the horizontal line indicate a comparison between the 2 groups. a–d is a bar chart comparing the C-FOS expression levels of the hypothalamic A–D nucleus (* P <0.05, ** P <0.01, **** P <0.001, ns: no significance).
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<t>C-FOS</t> expression of hypothalamic nuclei. ( A ) Arc nuclei (×200). ( B ) VMN nuclei (×200). ( C ) DM nuclei (×200). ( D ) PV nuclei (×200). Scale bar=100 μm. ( E ) The 2 ends of the horizontal line indicate a comparison between the 2 groups. a–d is a bar chart comparing the C-FOS expression levels of the hypothalamic A–D nucleus (* P <0.05, ** P <0.01, **** P <0.001, ns: no significance).
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C-FOS expression of hypothalamic nuclei. ( A ) Arc nuclei (×200). ( B ) VMN nuclei (×200). ( C ) DM nuclei (×200). ( D ) PV nuclei (×200). Scale bar=100 μm. ( E ) The 2 ends of the horizontal line indicate a comparison between the 2 groups. a–d is a bar chart comparing the C-FOS expression levels of the hypothalamic A–D nucleus (* P <0.05, ** P <0.01, **** P <0.001, ns: no significance).

Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

Article Title: Construction of a Mex3c Gene-Deficient Mouse Model to Study C-FOS Expression in Hypothalamic Nuclei and Observe Morphological Differences in Embryonic Neural Tube Development

doi: 10.12659/MSM.927334

Figure Lengend Snippet: C-FOS expression of hypothalamic nuclei. ( A ) Arc nuclei (×200). ( B ) VMN nuclei (×200). ( C ) DM nuclei (×200). ( D ) PV nuclei (×200). Scale bar=100 μm. ( E ) The 2 ends of the horizontal line indicate a comparison between the 2 groups. a–d is a bar chart comparing the C-FOS expression levels of the hypothalamic A–D nucleus (* P <0.05, ** P <0.01, **** P <0.001, ns: no significance).

Article Snippet: We performed conventional dewaxing and hydration and used citrate antigen repair solution for high-pressure repair for 10 min. We used 3% H 2 O 2 for removal of endogenous peroxidase, then goat serum blocking and addition of mouse-derived C-FOS primary antibody (1: 300, Proteintech, USA) were performed overnight at 4°C.

Techniques: Expressing