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Figure 2. Effect of miR204-5p mimics on mRNA and protein levels of transcription factors Fos-like anti- gen 2 (FOSL2) and <t>Forkhead</t> <t>Box</t> <t>C1</t> <t>(FOXC1),</t> homeobox transcription factor Myeloid Ectopic Viral Integration Site 2 (Meis2), and adipocyte differentiation regulator peroxisome proliferator-activated receptor gamma (PPARγ) in primary human limbal epithelial cells (LECs) using normal medium
Foxc1 Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 2. Effect of miR204-5p mimics on mRNA and protein levels of transcription factors Fos-like anti- gen 2 (FOSL2) and <t>Forkhead</t> <t>Box</t> <t>C1</t> <t>(FOXC1),</t> homeobox transcription factor Myeloid Ectopic Viral Integration Site 2 (Meis2), and adipocyte differentiation regulator peroxisome proliferator-activated receptor gamma (PPARγ) in primary human limbal epithelial cells (LECs) using normal medium
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Effect of miR204-5p mimics on mRNA and protein levels of transcription factors Fos-like antigen 2 (FOSL2) and <t>Forkhead</t> <t>Box</t> <t>C1</t> <t>(FOXC1),</t> homeobox transcription factor Myeloid Ectopic Viral Integration Site 2 (Meis2), and adipocyte differentiation regulator peroxisome proliferator-activated receptor gamma (PPARγ) in primary human limbal epithelial cells (LECs) using normal medium (Ctrl) or medium containing lipopolysaccharide (LPS). Data are represented as means ± SEMs. The statistical analysis was performed using a two-way ANOVA, followed by Bonferroni’s test. p -values below 0.05 were considered statistically significant. ( A ) FOSL2 and FOXC1 mRNA levels were downregulated in the cells transfected with the miR204-5p mimics in normal medium ( p = 0.006, p = 0.002). Under LPS-induced inflammation, mRNA levels of FOXC1 were downregulated following transfection with the miR204-5p mimics ( p = 0.003). Nevertheless, none of the levels of the other mRNAs examined changed significantly upon the use of the miR204-5p mimics and/or during LPS-induced inflammation ( p ≥ 0.07). ( B ) FOSL2 protein levels were significantly reduced in miR-204-5p-transfected cells upon the treatment with LPS ( p = 0.008). FOXC1 protein levels were significantly reduced in the presence of LPS in the miR204-5p-transfected cells compared to those in control transfection ( p = 0.020). Each dot represents an individual donor.
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Effect of miR204-5p mimics on mRNA and protein levels of transcription factors Fos-like antigen 2 (FOSL2) and <t>Forkhead</t> <t>Box</t> <t>C1</t> <t>(FOXC1),</t> homeobox transcription factor Myeloid Ectopic Viral Integration Site 2 (Meis2), and adipocyte differentiation regulator peroxisome proliferator-activated receptor gamma (PPARγ) in primary human limbal epithelial cells (LECs) using normal medium (Ctrl) or medium containing lipopolysaccharide (LPS). Data are represented as means ± SEMs. The statistical analysis was performed using a two-way ANOVA, followed by Bonferroni’s test. p -values below 0.05 were considered statistically significant. ( A ) FOSL2 and FOXC1 mRNA levels were downregulated in the cells transfected with the miR204-5p mimics in normal medium ( p = 0.006, p = 0.002). Under LPS-induced inflammation, mRNA levels of FOXC1 were downregulated following transfection with the miR204-5p mimics ( p = 0.003). Nevertheless, none of the levels of the other mRNAs examined changed significantly upon the use of the miR204-5p mimics and/or during LPS-induced inflammation ( p ≥ 0.07). ( B ) FOSL2 protein levels were significantly reduced in miR-204-5p-transfected cells upon the treatment with LPS ( p = 0.008). FOXC1 protein levels were significantly reduced in the presence of LPS in the miR204-5p-transfected cells compared to those in control transfection ( p = 0.020). Each dot represents an individual donor.
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Effect of miR204-5p mimics on mRNA and protein levels of transcription factors Fos-like antigen 2 (FOSL2) and <t>Forkhead</t> <t>Box</t> <t>C1</t> <t>(FOXC1),</t> homeobox transcription factor Myeloid Ectopic Viral Integration Site 2 (Meis2), and adipocyte differentiation regulator peroxisome proliferator-activated receptor gamma (PPARγ) in primary human limbal epithelial cells (LECs) using normal medium (Ctrl) or medium containing lipopolysaccharide (LPS). Data are represented as means ± SEMs. The statistical analysis was performed using a two-way ANOVA, followed by Bonferroni’s test. p -values below 0.05 were considered statistically significant. ( A ) FOSL2 and FOXC1 mRNA levels were downregulated in the cells transfected with the miR204-5p mimics in normal medium ( p = 0.006, p = 0.002). Under LPS-induced inflammation, mRNA levels of FOXC1 were downregulated following transfection with the miR204-5p mimics ( p = 0.003). Nevertheless, none of the levels of the other mRNAs examined changed significantly upon the use of the miR204-5p mimics and/or during LPS-induced inflammation ( p ≥ 0.07). ( B ) FOSL2 protein levels were significantly reduced in miR-204-5p-transfected cells upon the treatment with LPS ( p = 0.008). FOXC1 protein levels were significantly reduced in the presence of LPS in the miR204-5p-transfected cells compared to those in control transfection ( p = 0.020). Each dot represents an individual donor.
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antibodies against foxc1  (Proteintech)
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Effect of miR204-5p mimics on mRNA and protein levels of transcription factors Fos-like antigen 2 (FOSL2) and <t>Forkhead</t> <t>Box</t> <t>C1</t> <t>(FOXC1),</t> homeobox transcription factor Myeloid Ectopic Viral Integration Site 2 (Meis2), and adipocyte differentiation regulator peroxisome proliferator-activated receptor gamma (PPARγ) in primary human limbal epithelial cells (LECs) using normal medium (Ctrl) or medium containing lipopolysaccharide (LPS). Data are represented as means ± SEMs. The statistical analysis was performed using a two-way ANOVA, followed by Bonferroni’s test. p -values below 0.05 were considered statistically significant. ( A ) FOSL2 and FOXC1 mRNA levels were downregulated in the cells transfected with the miR204-5p mimics in normal medium ( p = 0.006, p = 0.002). Under LPS-induced inflammation, mRNA levels of FOXC1 were downregulated following transfection with the miR204-5p mimics ( p = 0.003). Nevertheless, none of the levels of the other mRNAs examined changed significantly upon the use of the miR204-5p mimics and/or during LPS-induced inflammation ( p ≥ 0.07). ( B ) FOSL2 protein levels were significantly reduced in miR-204-5p-transfected cells upon the treatment with LPS ( p = 0.008). FOXC1 protein levels were significantly reduced in the presence of LPS in the miR204-5p-transfected cells compared to those in control transfection ( p = 0.020). Each dot represents an individual donor.
Antibodies Against Foxc1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti foxc1  (Proteintech)
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Effect of miR204-5p mimics on mRNA and protein levels of transcription factors Fos-like antigen 2 (FOSL2) and <t>Forkhead</t> <t>Box</t> <t>C1</t> <t>(FOXC1),</t> homeobox transcription factor Myeloid Ectopic Viral Integration Site 2 (Meis2), and adipocyte differentiation regulator peroxisome proliferator-activated receptor gamma (PPARγ) in primary human limbal epithelial cells (LECs) using normal medium (Ctrl) or medium containing lipopolysaccharide (LPS). Data are represented as means ± SEMs. The statistical analysis was performed using a two-way ANOVA, followed by Bonferroni’s test. p -values below 0.05 were considered statistically significant. ( A ) FOSL2 and FOXC1 mRNA levels were downregulated in the cells transfected with the miR204-5p mimics in normal medium ( p = 0.006, p = 0.002). Under LPS-induced inflammation, mRNA levels of FOXC1 were downregulated following transfection with the miR204-5p mimics ( p = 0.003). Nevertheless, none of the levels of the other mRNAs examined changed significantly upon the use of the miR204-5p mimics and/or during LPS-induced inflammation ( p ≥ 0.07). ( B ) FOSL2 protein levels were significantly reduced in miR-204-5p-transfected cells upon the treatment with LPS ( p = 0.008). FOXC1 protein levels were significantly reduced in the presence of LPS in the miR204-5p-transfected cells compared to those in control transfection ( p = 0.020). Each dot represents an individual donor.
Anti Foxc1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 2. Effect of miR204-5p mimics on mRNA and protein levels of transcription factors Fos-like anti- gen 2 (FOSL2) and Forkhead Box C1 (FOXC1), homeobox transcription factor Myeloid Ectopic Viral Integration Site 2 (Meis2), and adipocyte differentiation regulator peroxisome proliferator-activated receptor gamma (PPARγ) in primary human limbal epithelial cells (LECs) using normal medium

Journal: International journal of molecular sciences

Article Title: Effect of MiRNA 204-5P Mimics and Lipopolysaccharide-Induced Inflammation on Transcription Factor Levels, Cell Maintenance, and Retinoic Acid Signaling in Primary Limbal Epithelial Cells.

doi: 10.3390/ijms26083809

Figure Lengend Snippet: Figure 2. Effect of miR204-5p mimics on mRNA and protein levels of transcription factors Fos-like anti- gen 2 (FOSL2) and Forkhead Box C1 (FOXC1), homeobox transcription factor Myeloid Ectopic Viral Integration Site 2 (Meis2), and adipocyte differentiation regulator peroxisome proliferator-activated receptor gamma (PPARγ) in primary human limbal epithelial cells (LECs) using normal medium

Article Snippet: Manufacturer RXRa Polyclonal 1:2000 21218-1-AP Proteintech, Planegg-Martinsried, Germany RDH10 Polyclonal 1:1000 14644-1-AP Proteintech, Planegg-Martinsried, Germany FABP5 Polyclonal 1:1000 12348-1-AP Proteintech, Planegg-Martinsried, Germany FOSL2 Monoclonal 1:3000 TA809660S OriGene Technologies, Rockville, MD, USA FOXC1 Polyclonal 1:500 55365-1-AP Proteintech, Planegg-Martinsried, Germany Keratin K3/K76 Monoclonal 1:500 CBL218 MERCK, Darmstadt, Germany Keratin 12 (E-8) Monoclonal 1:2000 sc-515882 Santa Cruz biotechnology, Dallas, TX, USA MMP2 Monoclonal 1:250 66366-1-Ig Proteintech, Planegg-Martinsried, Germany β-actin Polyclonal 1:10,000 Ab8227 Abcam, Cambridge, UK

Techniques:

Figure 7. The effects of miR-204-5p mimics on genes involved in limbal epithelial cell (LEC) differ- entiation, migration, and inflammatory responses under both normal and inflammatory conditions are summarized. The connections and interactions between these genes were explored using the STRING database. CHST6: carbohydrate sulfotransferase 6; FABP5: Fatty-Acid-Binding Protein 5; FOXC1: Forkhead Box C1; FOSL2: Fos-like antigen 2; IL-6: interleukin-6; KRT12: keratin 12; KRT3: Keratin 3; MMP2: Matrix Metallopeptidase 2; PPARD: peroxisome proliferator-activated receptor delta; RXRA: Retinoid X Receptor Alpha; RBP2: Retinol-Binding Protein 2; RDH10: Retinol Dehydro- genase 10. Each color represents a protein and arrows represent the changes that we observed upon overexpression of miR-204-5p (upwards: upregulation and downwards: down regulation).

Journal: International journal of molecular sciences

Article Title: Effect of MiRNA 204-5P Mimics and Lipopolysaccharide-Induced Inflammation on Transcription Factor Levels, Cell Maintenance, and Retinoic Acid Signaling in Primary Limbal Epithelial Cells.

doi: 10.3390/ijms26083809

Figure Lengend Snippet: Figure 7. The effects of miR-204-5p mimics on genes involved in limbal epithelial cell (LEC) differ- entiation, migration, and inflammatory responses under both normal and inflammatory conditions are summarized. The connections and interactions between these genes were explored using the STRING database. CHST6: carbohydrate sulfotransferase 6; FABP5: Fatty-Acid-Binding Protein 5; FOXC1: Forkhead Box C1; FOSL2: Fos-like antigen 2; IL-6: interleukin-6; KRT12: keratin 12; KRT3: Keratin 3; MMP2: Matrix Metallopeptidase 2; PPARD: peroxisome proliferator-activated receptor delta; RXRA: Retinoid X Receptor Alpha; RBP2: Retinol-Binding Protein 2; RDH10: Retinol Dehydro- genase 10. Each color represents a protein and arrows represent the changes that we observed upon overexpression of miR-204-5p (upwards: upregulation and downwards: down regulation).

Article Snippet: Manufacturer RXRa Polyclonal 1:2000 21218-1-AP Proteintech, Planegg-Martinsried, Germany RDH10 Polyclonal 1:1000 14644-1-AP Proteintech, Planegg-Martinsried, Germany FABP5 Polyclonal 1:1000 12348-1-AP Proteintech, Planegg-Martinsried, Germany FOSL2 Monoclonal 1:3000 TA809660S OriGene Technologies, Rockville, MD, USA FOXC1 Polyclonal 1:500 55365-1-AP Proteintech, Planegg-Martinsried, Germany Keratin K3/K76 Monoclonal 1:500 CBL218 MERCK, Darmstadt, Germany Keratin 12 (E-8) Monoclonal 1:2000 sc-515882 Santa Cruz biotechnology, Dallas, TX, USA MMP2 Monoclonal 1:250 66366-1-Ig Proteintech, Planegg-Martinsried, Germany β-actin Polyclonal 1:10,000 Ab8227 Abcam, Cambridge, UK

Techniques: Migration, Binding Assay, Over Expression

Effect of miR204-5p mimics on mRNA and protein levels of transcription factors Fos-like antigen 2 (FOSL2) and Forkhead Box C1 (FOXC1), homeobox transcription factor Myeloid Ectopic Viral Integration Site 2 (Meis2), and adipocyte differentiation regulator peroxisome proliferator-activated receptor gamma (PPARγ) in primary human limbal epithelial cells (LECs) using normal medium (Ctrl) or medium containing lipopolysaccharide (LPS). Data are represented as means ± SEMs. The statistical analysis was performed using a two-way ANOVA, followed by Bonferroni’s test. p -values below 0.05 were considered statistically significant. ( A ) FOSL2 and FOXC1 mRNA levels were downregulated in the cells transfected with the miR204-5p mimics in normal medium ( p = 0.006, p = 0.002). Under LPS-induced inflammation, mRNA levels of FOXC1 were downregulated following transfection with the miR204-5p mimics ( p = 0.003). Nevertheless, none of the levels of the other mRNAs examined changed significantly upon the use of the miR204-5p mimics and/or during LPS-induced inflammation ( p ≥ 0.07). ( B ) FOSL2 protein levels were significantly reduced in miR-204-5p-transfected cells upon the treatment with LPS ( p = 0.008). FOXC1 protein levels were significantly reduced in the presence of LPS in the miR204-5p-transfected cells compared to those in control transfection ( p = 0.020). Each dot represents an individual donor.

Journal: International Journal of Molecular Sciences

Article Title: Effect of MiRNA 204-5P Mimics and Lipopolysaccharide-Induced Inflammation on Transcription Factor Levels, Cell Maintenance, and Retinoic Acid Signaling in Primary Limbal Epithelial Cells

doi: 10.3390/ijms26083809

Figure Lengend Snippet: Effect of miR204-5p mimics on mRNA and protein levels of transcription factors Fos-like antigen 2 (FOSL2) and Forkhead Box C1 (FOXC1), homeobox transcription factor Myeloid Ectopic Viral Integration Site 2 (Meis2), and adipocyte differentiation regulator peroxisome proliferator-activated receptor gamma (PPARγ) in primary human limbal epithelial cells (LECs) using normal medium (Ctrl) or medium containing lipopolysaccharide (LPS). Data are represented as means ± SEMs. The statistical analysis was performed using a two-way ANOVA, followed by Bonferroni’s test. p -values below 0.05 were considered statistically significant. ( A ) FOSL2 and FOXC1 mRNA levels were downregulated in the cells transfected with the miR204-5p mimics in normal medium ( p = 0.006, p = 0.002). Under LPS-induced inflammation, mRNA levels of FOXC1 were downregulated following transfection with the miR204-5p mimics ( p = 0.003). Nevertheless, none of the levels of the other mRNAs examined changed significantly upon the use of the miR204-5p mimics and/or during LPS-induced inflammation ( p ≥ 0.07). ( B ) FOSL2 protein levels were significantly reduced in miR-204-5p-transfected cells upon the treatment with LPS ( p = 0.008). FOXC1 protein levels were significantly reduced in the presence of LPS in the miR204-5p-transfected cells compared to those in control transfection ( p = 0.020). Each dot represents an individual donor.

Article Snippet: FOXC1 , Polyclonal , 1:500 , 55365-1-AP , Proteintech, Planegg-Martinsried, Germany.

Techniques: Transfection, Control

The effects of miR-204-5p mimics on genes involved in limbal epithelial cell (LEC) differentiation, migration, and inflammatory responses under both normal and inflammatory conditions are summarized. The connections and interactions between these genes were explored using the STRING database. CHST6: carbohydrate sulfotransferase 6; FABP5: Fatty-Acid-Binding Protein 5; FOXC1: Forkhead Box C1; FOSL2: Fos-like antigen 2; IL-6: interleukin-6; KRT12: keratin 12; KRT3: Keratin 3; MMP2: Matrix Metallopeptidase 2; PPARD: peroxisome proliferator-activated receptor delta; RXRA: Retinoid X Receptor Alpha; RBP2: Retinol-Binding Protein 2; RDH10: Retinol Dehydrogenase 10. Each color represents a protein and arrows represent the changes that we observed upon overexpression of miR-204-5p (upwards: upregulation and downwards: down regulation).

Journal: International Journal of Molecular Sciences

Article Title: Effect of MiRNA 204-5P Mimics and Lipopolysaccharide-Induced Inflammation on Transcription Factor Levels, Cell Maintenance, and Retinoic Acid Signaling in Primary Limbal Epithelial Cells

doi: 10.3390/ijms26083809

Figure Lengend Snippet: The effects of miR-204-5p mimics on genes involved in limbal epithelial cell (LEC) differentiation, migration, and inflammatory responses under both normal and inflammatory conditions are summarized. The connections and interactions between these genes were explored using the STRING database. CHST6: carbohydrate sulfotransferase 6; FABP5: Fatty-Acid-Binding Protein 5; FOXC1: Forkhead Box C1; FOSL2: Fos-like antigen 2; IL-6: interleukin-6; KRT12: keratin 12; KRT3: Keratin 3; MMP2: Matrix Metallopeptidase 2; PPARD: peroxisome proliferator-activated receptor delta; RXRA: Retinoid X Receptor Alpha; RBP2: Retinol-Binding Protein 2; RDH10: Retinol Dehydrogenase 10. Each color represents a protein and arrows represent the changes that we observed upon overexpression of miR-204-5p (upwards: upregulation and downwards: down regulation).

Article Snippet: FOXC1 , Polyclonal , 1:500 , 55365-1-AP , Proteintech, Planegg-Martinsried, Germany.

Techniques: Migration, Binding Assay, Over Expression

Primers used for qPCR analysis.

Journal: International Journal of Molecular Sciences

Article Title: Effect of MiRNA 204-5P Mimics and Lipopolysaccharide-Induced Inflammation on Transcription Factor Levels, Cell Maintenance, and Retinoic Acid Signaling in Primary Limbal Epithelial Cells

doi: 10.3390/ijms26083809

Figure Lengend Snippet: Primers used for qPCR analysis.

Article Snippet: FOXC1 , Polyclonal , 1:500 , 55365-1-AP , Proteintech, Planegg-Martinsried, Germany.

Techniques:

Primary antibodies used for Western blot analysis.

Journal: International Journal of Molecular Sciences

Article Title: Effect of MiRNA 204-5P Mimics and Lipopolysaccharide-Induced Inflammation on Transcription Factor Levels, Cell Maintenance, and Retinoic Acid Signaling in Primary Limbal Epithelial Cells

doi: 10.3390/ijms26083809

Figure Lengend Snippet: Primary antibodies used for Western blot analysis.

Article Snippet: FOXC1 , Polyclonal , 1:500 , 55365-1-AP , Proteintech, Planegg-Martinsried, Germany.

Techniques: Western Blot