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Transcriptome analysis to determine the mechanism of EBP-bFGF/ECM-mediated repair in rats with cerebral ischemia. (A) Gene Ontology (GO) analysis of EBP-bFGF/ECM hydrogel at 5 d after implantation. (B) Differential gene expression of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. (C) Forty significantly different genes related to regeneration in the ischemic brain. n = 3. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. cGMP, guanosine 3′,5′-monophosphate; PKG, cGMP-dependent protein kinase; TGF-β, transforming growth factor–β; NOD, nonobese diabetic. (E) Western blot of <t>Nrg1/p-AKT</t> pathway in the right hemisphere at 5 d. n = 3.
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Transcriptome analysis to determine the mechanism of EBP-bFGF/ECM-mediated repair in rats with cerebral ischemia. (A) Gene Ontology (GO) analysis of EBP-bFGF/ECM hydrogel at 5 d after implantation. (B) Differential gene expression of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. (C) Forty significantly different genes related to regeneration in the ischemic brain. n = 3. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. cGMP, guanosine 3′,5′-monophosphate; PKG, cGMP-dependent protein kinase; TGF-β, transforming growth factor–β; NOD, nonobese diabetic. (E) Western blot of <t>Nrg1/p-AKT</t> pathway in the right hemisphere at 5 d. n = 3.
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Transcriptome analysis to determine the mechanism of EBP-bFGF/ECM-mediated repair in rats with cerebral ischemia. (A) Gene Ontology (GO) analysis of EBP-bFGF/ECM hydrogel at 5 d after implantation. (B) Differential gene expression of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. (C) Forty significantly different genes related to regeneration in the ischemic brain. n = 3. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. cGMP, guanosine 3′,5′-monophosphate; PKG, cGMP-dependent protein kinase; TGF-β, transforming growth factor–β; NOD, nonobese diabetic. (E) Western blot of <t>Nrg1/p-AKT</t> pathway in the right hemisphere at 5 d. n = 3.
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https://www.bioz.com/result/rabbit anti nrg1/product/Proteintech
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Transcriptome analysis to determine the mechanism of EBP-bFGF/ECM-mediated repair in rats with cerebral ischemia. (A) Gene Ontology (GO) analysis of EBP-bFGF/ECM hydrogel at 5 d after implantation. (B) Differential gene expression of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. (C) Forty significantly different genes related to regeneration in the ischemic brain. n = 3. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. cGMP, guanosine 3′,5′-monophosphate; PKG, cGMP-dependent protein kinase; TGF-β, transforming growth factor–β; NOD, nonobese diabetic. (E) Western blot of <t>Nrg1/p-AKT</t> pathway in the right hemisphere at 5 d. n = 3.
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Transcriptome analysis to determine the mechanism of EBP-bFGF/ECM-mediated repair in rats with cerebral ischemia. (A) Gene Ontology (GO) analysis of EBP-bFGF/ECM hydrogel at 5 d after implantation. (B) Differential gene expression of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. (C) Forty significantly different genes related to regeneration in the ischemic brain. n = 3. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. cGMP, guanosine 3′,5′-monophosphate; PKG, cGMP-dependent protein kinase; TGF-β, transforming growth factor–β; NOD, nonobese diabetic. (E) Western blot of <t>Nrg1/p-AKT</t> pathway in the right hemisphere at 5 d. n = 3.
Neuregulin 1 Nrg 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/neuregulin 1 nrg 1/product/Proteintech
Average 93 stars, based on 1 article reviews
neuregulin 1 nrg 1 - by Bioz Stars, 2025-06
93/100 stars
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Transcriptome analysis to determine the mechanism of EBP-bFGF/ECM-mediated repair in rats with cerebral ischemia. (A) Gene Ontology (GO) analysis of EBP-bFGF/ECM hydrogel at 5 d after implantation. (B) Differential gene expression of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. (C) Forty significantly different genes related to regeneration in the ischemic brain. n = 3. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. cGMP, guanosine 3′,5′-monophosphate; PKG, cGMP-dependent protein kinase; TGF-β, transforming growth factor–β; NOD, nonobese diabetic. (E) Western blot of Nrg1/p-AKT pathway in the right hemisphere at 5 d. n = 3.

Journal: Biomaterials Research

Article Title: Engineered Basic Fibroblast Growth Factor Specifically Bonded with Injectable Extracellular Matrix Hydrogel for the Functional Restoration of Cerebral Ischemia in Rats

doi: 10.34133/bmr.0020

Figure Lengend Snippet: Transcriptome analysis to determine the mechanism of EBP-bFGF/ECM-mediated repair in rats with cerebral ischemia. (A) Gene Ontology (GO) analysis of EBP-bFGF/ECM hydrogel at 5 d after implantation. (B) Differential gene expression of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. (C) Forty significantly different genes related to regeneration in the ischemic brain. n = 3. (D) Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of PBS, ECM, and EBP-bFGF/ECM hydrogels at 5 d after implantation. cGMP, guanosine 3′,5′-monophosphate; PKG, cGMP-dependent protein kinase; TGF-β, transforming growth factor–β; NOD, nonobese diabetic. (E) Western blot of Nrg1/p-AKT pathway in the right hemisphere at 5 d. n = 3.

Article Snippet: The membranes were closed by tris-buffered saline with Tween with 5% skimmed milk powder for 2 h at 25 °C and incubated with the following primary antibody for 4 °C overnight: anti-bFGF antibody (#sc74412, SCBT; 1:1,000), anti-neuregulin-1 (Nrg1) antibody (#27455-1-AP, Proteintech; 1:5,000), anti-AKT (#A17909, ABclonal; 1:1,000)/anti-p-AKT (#AP0140, ABclonal; 1:1,000), and anti-β-actin rabbit monoclonal antibody (#AC038, ABclonal; 1:5,000).

Techniques: Expressing, Western Blot