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Serum anti-SARS-CoV-2 S-RBD IgM ( A ), IgG ( B ), anti-SARS-CoV-2 N protein IgM ( C ), and IgG ( D ) levels of patients with COVID-19 over weeks 1–6 after symptom onset. These patients were subgrouped based on pneumonia diagnosis. The anti-SARS-CoV-2 S-RBD IgM ( E ), IgG ( F ), anti-SARS-CoV-2 N protein IgM ( G ), and IgG ( H ) levels of the subgrouped patients were determined. The patients were also subgrouped based on the duration of hospitalization (≥ 21 d vs. < 21 d). The anti-SARS-CoV-2 S-RBD IgM ( I ), IgG ( J ), anti-SARS-CoV-2 N protein IgM ( K ), and IgG ( L ) levels of the subgrouped patients are shown. * p < 0.05

Journal: Infection

Article Title: SARS-CoV-2 nucleocapsid protein, rather than spike protein, triggers a cytokine storm originating from lung epithelial cells in patients with COVID-19

doi: 10.1007/s15010-023-02142-4

Figure Lengend Snippet: Serum anti-SARS-CoV-2 S-RBD IgM ( A ), IgG ( B ), anti-SARS-CoV-2 N protein IgM ( C ), and IgG ( D ) levels of patients with COVID-19 over weeks 1–6 after symptom onset. These patients were subgrouped based on pneumonia diagnosis. The anti-SARS-CoV-2 S-RBD IgM ( E ), IgG ( F ), anti-SARS-CoV-2 N protein IgM ( G ), and IgG ( H ) levels of the subgrouped patients were determined. The patients were also subgrouped based on the duration of hospitalization (≥ 21 d vs. < 21 d). The anti-SARS-CoV-2 S-RBD IgM ( I ), IgG ( J ), anti-SARS-CoV-2 N protein IgM ( K ), and IgG ( L ) levels of the subgrouped patients are shown. * p < 0.05

Article Snippet: SARS-CoV-2 IgG and IgM titers were determined using Anti-SARS-CoV-2 S receptor-binding domain (RBD) protein (wild-type) Human IgG ELISA Kit (KE30003; Proteintech, Rosemont, IL, USA), Anti-SARS-CoV-2 S-RBD protein (wild-type) Human IgM ELISA Kit (KE30004; Proteintech), Anti-SARS-CoV-2 N protein (wild-type) Human IgG (Proteintech, Catalog No. KE30001), and Anti-SARS-CoV-2 N protein (wild-type) Human IgM (Proteintech, Catalog No. KE30002), respectively, according to the manufacturer's instructions.

Techniques: