Journal: Frontiers in Cell and Developmental Biology

Article Title: Comprehensive analysis and validation of autophagy-related gene in rheumatoid arthritis

doi: 10.3389/fcell.2025.1563911

Figure Lengend Snippet: The expression of IRF4 in RA synovial tissues and cells. (A) The expression of IRF4 in RA and OA synovial tissues observed by immunohistochemistry (n = 4 in each group); (B) The expression of IRF4 in RA and OA synovial tissues observed by qRT-PCR (n = 3 in each group); (C) The expression of IRF4 in RA and OA synovial cells observed by qRT-PCR (n = 3 in each group). ** indicate p < 0.01.

Article Snippet: The primary antibody used was an anti-IRF4 antibody (Proteintech).

Techniques: Expressing, Immunohistochemistry, Quantitative RT-PCR

Journal: Frontiers in Cell and Developmental Biology

Article Title: Comprehensive analysis and validation of autophagy-related gene in rheumatoid arthritis

doi: 10.3389/fcell.2025.1563911

Figure Lengend Snippet: Effects of IRF4 silencing on MH7A. (A) Proliferative ability of MH7A with the above transfection, as determined by RTCA; (B) Migration of MH7A with the above transfection, as determined by scratch healing experiments; (C) Death of MH7A with the above transfection, as determined by flow cytometry; (D) The level of Beclin with the above transfection, as determined by WB; (E) The mRNA level of Beclin with the above transfection, as determined by qRT-PCR. The data were presented as mean ± SD. **, ***, **** indicate p < 0.01, p < 0.001, p < 0.0001, respectively. All experiments were repeated 3 times.

Article Snippet: The primary antibody used was an anti-IRF4 antibody (Proteintech).

Techniques: Transfection, Migration, Flow Cytometry, Quantitative RT-PCR

Journal: Frontiers in Cell and Developmental Biology

Article Title: Comprehensive analysis and validation of autophagy-related gene in rheumatoid arthritis

doi: 10.3389/fcell.2025.1563911

Figure Lengend Snippet:

Article Snippet: The primary antibody used was an anti-IRF4 antibody (Proteintech).

Techniques: Sequencing

Journal: Mediators of Inflammation

Article Title: MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression

doi: 10.1155/2017/6249685

Figure Lengend Snippet: Expression of IRF4 in three groups. (a) Lung tissues were stained for immunohistochemistry (anti-IRF4) of the three groups. Histological analyses of lungs from the severe and conventional groups exhibited markedly enhanced cells with stained IRF4 compared with those of the saline group. (b) Western blot analyses detected IRF4 protein expression in the lungs of the three groups. The IRF4 protein expression from the severe and conventional groups were higher than that from the saline group. (c) Western blot analyses detected IRF4 protein expression in the splenocytes of the three groups. The IRF4 protein expression from the severe and conventional groups were higher than that from the saline group.

Article Snippet: Lung tissues were stained for immunohistochemistry (neutrophil-specific antibody (anti-Gr1, Biolegend), eosinophil antibody (anti-ECP, Biorbyt), IL-17A antibody (Proteintech), IL-4 antibody (ABBIOTEC), MBD2 antibody (Abcam), and IRF4 antibody (Proteintech)), and 2~4 H&E stained sections per group were chosen under single-blind conditions to detect and localize NEU, EOS, IL-17A, IL-4, MBD2, and IRF4 protein expression.

Techniques: Expressing, Staining, Immunohistochemistry, Western Blot

Journal: Mediators of Inflammation

Article Title: MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression

doi: 10.1155/2017/6249685

Figure Lengend Snippet: IL-17 and MBD2 expression under IRF4 gene silencing (I(−)) or overexpression (I(+)). Under I(−), IL-17 and IRF4 protein expression were significantly lower than that of the empty transfection group (I(0)) according to Western blot analyses; under I(+), IL-17 and IRF4 protein expression were markedly increased compared to that of I(0). Under I(−) or I(+), MBD2 protein expression showed no significant difference.

Article Snippet: Lung tissues were stained for immunohistochemistry (neutrophil-specific antibody (anti-Gr1, Biolegend), eosinophil antibody (anti-ECP, Biorbyt), IL-17A antibody (Proteintech), IL-4 antibody (ABBIOTEC), MBD2 antibody (Abcam), and IRF4 antibody (Proteintech)), and 2~4 H&E stained sections per group were chosen under single-blind conditions to detect and localize NEU, EOS, IL-17A, IL-4, MBD2, and IRF4 protein expression.

Techniques: Expressing, Over Expression, Transfection, Western Blot

Journal: Mediators of Inflammation

Article Title: MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression

doi: 10.1155/2017/6249685

Figure Lengend Snippet: IL-17 expression and Th17 cell differentiation under joint MBD2 and IRF4 gene silencing or overexpression. (a) Under joint M(−)/I(−), IL-17 protein expression was significantly the lowest according to Western blot analyses; under joint M(−)/I(0), IL-17 protein expression was higher than M(−)/I(−). While under joint M(+)/I(+), IL-17 protein expression was significantly the highest; under joint M(+)/I(0), IL-17 protein expression was lower than M(+)/I(+). IL-17 protein expression showed no significant difference between the control group, M(0)/I(0), M(−)/I(+), and M(+)/I(−). (b) Under joint M(−)/I(−), Th17 cell differentiation was significantly the lowest according to flow cytometry analyses; under joint M(−)/I(0), Th17 cell differentiation was higher than M(−)/I(−). Under joint M(+)/I(+), Th17 cell differentiation was significantly the highest; under jointM(+)/I(0), Th17 cell differentiation was lower than M(+)/I(+). No significant difference in Th17 cell differentiation was observed between the control group, M(0)/I(0), M(−)/I(+), and M(+)/I(−).

Article Snippet: Lung tissues were stained for immunohistochemistry (neutrophil-specific antibody (anti-Gr1, Biolegend), eosinophil antibody (anti-ECP, Biorbyt), IL-17A antibody (Proteintech), IL-4 antibody (ABBIOTEC), MBD2 antibody (Abcam), and IRF4 antibody (Proteintech)), and 2~4 H&E stained sections per group were chosen under single-blind conditions to detect and localize NEU, EOS, IL-17A, IL-4, MBD2, and IRF4 protein expression.

Techniques: Expressing, Cell Differentiation, Over Expression, Western Blot, Flow Cytometry

Journal: Bioengineering & Translational Medicine

Article Title: Macrophage‐targeted delivery of siRNA to silence Mecp2 gene expression attenuates pulmonary fibrosis

doi: 10.1002/btm2.10280

Figure Lengend Snippet: Primers for RT‐PCR

Article Snippet: Antibodies against collagen I, Irf4, Gapdh, and β‐actin were purchased from Proteintech, and antibodies against Ym1 were purchased from Thermo Fisher Scientific.

Techniques: Sequencing

Journal: Bioengineering & Translational Medicine

Article Title: Macrophage‐targeted delivery of siRNA to silence Mecp2 gene expression attenuates pulmonary fibrosis

doi: 10.1002/btm2.10280

Figure Lengend Snippet: Mecp2 regulates M2 polarization via Irf4. (a and b) Representative images of coimmunostaining for MECP2 and IRF4 in BALF (a) and lung sections (b) from IPF patients and control subjects. The nuclei were stained blue by DAPI, and the images were taken at ×400 magnification. A total of five patients with IPF and five control subjects were analyzed. (c) Western blotting analysis of Irf4 expression in BMDMs after IL‐4 stimulation. Left panel: Representative Western blotting images. Right panel: Bar graphs showing the data from three replicates. (d) RT‐PCR analysis of the expression of Mecp2 in BMDMs following IL‐4 treatment. * p < 0.05; ** p < 0.01. BALF, bronchoalveolar lavage fluid; BMDMs, bone marrow‐derived macrophages; IPF, idiopathic pulmonary fibrosis; IRF4, interferon regulatory factor 4; Mecp2, methyl‐CpG‐binding protein 2; RT‐PCR, reverse transcription‐polymerase chain reaction; Scr: scramble

Article Snippet: Antibodies against collagen I, Irf4, Gapdh, and β‐actin were purchased from Proteintech, and antibodies against Ym1 were purchased from Thermo Fisher Scientific.

Techniques: Staining, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Derivative Assay, Binding Assay