Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: Information of primary antibodies used in the present study.

Article Snippet: Anti CD147 mouse monoclonal antibody , 66443-1-AP , ProteinTech Group, Inc. , 1:5000 /1:200.

Techniques:

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: Primer sequence and promoter primer sequence.

Article Snippet: Anti CD147 mouse monoclonal antibody , 66443-1-AP , ProteinTech Group, Inc. , 1:5000 /1:200.

Techniques: Sequencing

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: CD147 overexpression correlates with poor prognosis and RAP1 co-expression in colorectal cancer. ( A , B ) CD147 mRNA expression levels in tumor vs. normal tissues across multiple cancers (UCSC Xena database) and specifically in colorectal cancer (CRC) (GEPIA database). ** P < 0.01, unpaired t-test. ( C ) Kaplan-Meier survival analysis of CRC patients stratified by CD147 expression (log-rank test, P < 0.05). ( D ) Representative immunohistochemical images from The Human Protein Atlas showing CD147 expression in normal colorectal tissue (negative) and CRC tissue (cytoplasmic/membrane staining). ( E , F ) Western blot analysis of CD147 protein levels in paired CRC and adjacent normal tissues ( n = 12). β-actin served as loading control. *** P < 0.001, paired t-test. Origin blots are presented in Supplementary Fig. 1. ( G , H ) Correlation analyses between CD147 and RAP1A/B mRNA expression using GEPIA (Pearson’s correlation, P < 0.05) and TIMER2.0 (no significant correlation). ( I ) qRT-PCR validation of CD147 and Rap1 expression correlation in 12 CRC tissues (Pearson’s correlation, P < 0.01).

Article Snippet: Anti CD147 mouse monoclonal antibody , 66443-1-AP , ProteinTech Group, Inc. , 1:5000 /1:200.

Techniques: Over Expression, Expressing, Immunohistochemical staining, Membrane, Staining, Western Blot, Control, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: CD147 expression validation and knockdown efficiency in CRC cell lines. ( A ) Immunofluorescence staining of CD147 in SW620 cells showing strong cytoplasmic expression (red) with partial membrane localization. Nuclei were counterstained with Hoechst 33,342 (blue). Scale bar: 30 μm. ( B ) Western blot analysis of CD147 protein levels in HCT116, SW620, and normal colon epithelial HCoEpiC cells. β-actin served as loading control ( n = 3 independent experiments). Original blots are presented in Supplementary Fig. 1. ( C ) qRT-PCR quantification of CD147 mRNA levels normalized to β-actin ( n = 3 independent experiments). ( D - F ) Lentiviral shRNA-mediated CD147 knockdown in HCT116 cells. ( D ) Representative Western blot of CD147 protein levels after transfection with three shRNAs (shCD147-1/2/3) or non-targeting control (shNC). Original blots are presented in Supplementary Fig. 1. ( E ) Densitometric quantification of CD147 protein expression from three independent experiments. ( F ) qRT-PCR analysis of CD147 mRNA levels post-knockdown (normalized to β-actin). Data are mean ± SD; *** P < 0.001 (Student’s t-test).

Article Snippet: Anti CD147 mouse monoclonal antibody , 66443-1-AP , ProteinTech Group, Inc. , 1:5000 /1:200.

Techniques: Expressing, Knockdown, Immunofluorescence, Staining, Membrane, Western Blot, Control, Quantitative RT-PCR, shRNA, Transfection

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: CD147 knockdown inhibits proliferation and induces apoptosis in CRC cells. ( A ) CCK-8 proliferation assay showing reduced viability of HCT116 and SW620 cells transfected with shCD147-1 (shCD147) compared to non-targeting control (shNC) at 48 h and 72 h ( n = 3, mean ± SD; *** P < 0.001, one-way ANOVA). ( B , C ) Colony formation assay in shCD147 and shNC groups. ( B ) Representative images of colonies (crystal violet staining). ( C ) Quantification of colony numbers ( n = 3; *** P < 0.001 vs. shNC, Student’s t-test). ( D - F ) Western blot analysis of proliferation/apoptosis-related proteins. ( D ) Representative blots of c-Myc, Bcl-2, and Bax in shCD147 and shNC groups. β-actin served as loading control. Original blots are presented in Supplementary Fig. 2. ( E , F ) Densitometric quantification of protein levels ( n = 3; ** P < 0.01, *** P < 0.001 vs. shNC, Student’s t-test). ( G , H ) Flow cytometry analysis of apoptosis. ( G ) Representative Annexin V-FITC/PI staining profiles. ( H ) Quantification of apoptotic cell percentages ( n = 3; *** P < 0.001 vs. shNC, Student’s t-test).

Article Snippet: Anti CD147 mouse monoclonal antibody , 66443-1-AP , ProteinTech Group, Inc. , 1:5000 /1:200.

Techniques: Knockdown, CCK-8 Assay, Proliferation Assay, Transfection, Control, Colony Assay, Staining, Western Blot, Flow Cytometry

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: CD147 knockdown suppresses migration, invasion, and epithelial-mesenchymal transition (EMT) in colorectal cancer cells. ( A , B ) Scratch wound healing assay in HCT116 and SW620 cells. ( A ) Representative images of wound closure at 24 h and 48 h post-scratching. ( B ) Quantification of wound closure rate ( n = 3; mean ± SD; ** P < 0.01, *** P < 0.001, vs. shNC, one-way ANOVA). ( C , D ) Transwell migration and invasion assays. ( C ) Representative images of migrated/invaded cells (crystal violet staining). ( D ) Quantification of migrated/invaded cells ( n = 3; *** P < 0.01 vs. shNC, Student’s t-test). ( E , F ) Western blot analysis of EMT-related proteins. ( E ) Representative blots of E-cadherin and N-cadherin in shCD147 and shNC groups. β-actin served as loading control. Original blots are presented in Supplementary Fig. 2. ( F ) Densitometric quantification of protein levels ( n = 3; ** P < 0.01, *** P < 0.001 vs. shNC, Student’s t-test).

Article Snippet: Anti CD147 mouse monoclonal antibody , 66443-1-AP , ProteinTech Group, Inc. , 1:5000 /1:200.

Techniques: Knockdown, Migration, Wound Healing Assay, Staining, Western Blot, Control

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: Rap1 overexpression reverses the expression of key proteins in CD147 knockdown mediated cell proliferation, apoptosis and EMT. ( A , B ) Western blot analysis of Rap1 and Rap1GAP protein levels in HCT116 and SW620 cells after CD147 knockdown (shCD147). β-actin served as a loading control. Data represent mean ± SD ( n = 3; ** P < 0.01, *** P < 0.001 vs. shNC, Student’s t-test). Original blots are presented in Supplementary Fig. 3. ( C ) qRT-PCR validation of Rap1 mRNA overexpression in CD147-knockdown cells. Expression normalized to β-actin ( n = 3; *** P < 0.001 vs. HCT116 or SW620, Student’s t-test). ( D , E ) Western blot analysis of c-Myc, Bcl-2, Bax, N-cadherin, and E-cadherin protein levels in CD147-Knockdown cells with Rap1 overexpression ( n = 3; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. shNC, # P < 0.05, ## P < 0.01, ### P < 0.001 vs. shCD147, one-way ANOVA). Original blots are presented in Supplementary Fig. 4.

Article Snippet: Anti CD147 mouse monoclonal antibody , 66443-1-AP , ProteinTech Group, Inc. , 1:5000 /1:200.

Techniques: Over Expression, Expressing, Knockdown, Western Blot, Control, Quantitative RT-PCR

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: Rap1 overexpression rescues the regulatory effects of CD147 knockdown on proliferation and apoptosis. ( A ) CCK-8 assay showing proliferation of HCT116 ( n = 3; *** P < 0.001 vs. shNC, ### P < 0.001 vs. shCD147, one-way ANOVA). ( B , C ) Colony formation assays in HCT116 cells. Colonies were counted and normalized to shCtrl ( n = 3; ** P < 0.01, *** P < 0.001 vs. shNC, ### P < 0.001 vs. shCD147, one-way ANOVA). ( D , E ) Flow cytometry analysis of apoptosis in HCT116 cells. Percentages of apoptotic cells are shown ( n = 3; * P < 0.05 vs. shNC, # P < 0.05 vs. shCD147, one-way ANOVA). Similar results were observed in SW620 cells.

Article Snippet: Anti CD147 mouse monoclonal antibody , 66443-1-AP , ProteinTech Group, Inc. , 1:5000 /1:200.

Techniques: Over Expression, Knockdown, CCK-8 Assay, Flow Cytometry

Journal: Scientific Reports

Article Title: CD147 regulates the Rap1 signaling pathway to promote proliferation, migration, and invasion, and inhibit apoptosis in colorectal cancer cells

doi: 10.1038/s41598-025-98266-8

Figure Lengend Snippet: Rap1 restores CD147-mediated migration and invasion in colorectal cancer cells. ( A , C ) Scratch wound healing assay in HCT116 cells. Healing rates were quantified at 24 h and 48 h ( n = 3; *** P < 0.001 vs. shNC, ## P < 0.01, ### P < 0.001 vs. shCD147, one-way ANOVA). ( B , D ) Transwell migration and Matrigel invasion assays. Migrated/invaded cells were counted and normalized to shCtrl ( n = 3; *** P < 0.001 vs. shNC, ### P < 0.001 vs. shCD147, one-way ANOVA). SW620 cells showed consistent trends. ( E ) Mechanistic diagram of CD147 promoting tumor progression through Rap1/Rap1GAP signaling in colorectal cancer cells.

Article Snippet: Anti CD147 mouse monoclonal antibody , 66443-1-AP , ProteinTech Group, Inc. , 1:5000 /1:200.

Techniques: Migration, Wound Healing Assay