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Apoa4, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apoa4 antibody  (Proteintech)
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DEPs coincident with the tissue expression. (A) Numbers of DEPs coincident with the tissue expression. (B) DEPs which were mainly expressed by enterocytes in single-cell RNA data of gastric tissue samples. (C) DEPs with high expression specificity in enterocyte in Human Protein Atlas data. (D) <t>APOA4</t> levels in the gastric tissue samples. The barplot is the statistical result of the optical density analysis of IHC images. These tissue samples include NAG ( n = 10), IM ( n = 16), and GC ( n = 16). * p < 0.05; ** p < 0.01. (E) DEPs which were mainly expressed by epithelial cells in single-cell RNA data. (F) Fold changes of DEPs across cell types in pre-GC vs gastritis/normal comparison group. (G) DEPs coincident with the RNA datasets, GSE55696 and GSE130823. (H–J) ROC curves of the random forest models using the signature, APOA4, SERPINA4, and GCA.
Apoa4 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DEPs coincident with the tissue expression. (A) Numbers of DEPs coincident with the tissue expression. (B) DEPs which were mainly expressed by enterocytes in single-cell RNA data of gastric tissue samples. (C) DEPs with high expression specificity in enterocyte in Human Protein Atlas data. (D) <t>APOA4</t> levels in the gastric tissue samples. The barplot is the statistical result of the optical density analysis of IHC images. These tissue samples include NAG ( n = 10), IM ( n = 16), and GC ( n = 16). * p < 0.05; ** p < 0.01. (E) DEPs which were mainly expressed by epithelial cells in single-cell RNA data. (F) Fold changes of DEPs across cell types in pre-GC vs gastritis/normal comparison group. (G) DEPs coincident with the RNA datasets, GSE55696 and GSE130823. (H–J) ROC curves of the random forest models using the signature, APOA4, SERPINA4, and GCA.
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DEPs coincident with the tissue expression. (A) Numbers of DEPs coincident with the tissue expression. (B) DEPs which were mainly expressed by enterocytes in single-cell RNA data of gastric tissue samples. (C) DEPs with high expression specificity in enterocyte in Human Protein Atlas data. (D) <t>APOA4</t> levels in the gastric tissue samples. The barplot is the statistical result of the optical density analysis of IHC images. These tissue samples include NAG ( n = 10), IM ( n = 16), and GC ( n = 16). * p < 0.05; ** p < 0.01. (E) DEPs which were mainly expressed by epithelial cells in single-cell RNA data. (F) Fold changes of DEPs across cell types in pre-GC vs gastritis/normal comparison group. (G) DEPs coincident with the RNA datasets, GSE55696 and GSE130823. (H–J) ROC curves of the random forest models using the signature, APOA4, SERPINA4, and GCA.
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gr3379814 4 apoa4 proteintech 17996 1 ap  (Proteintech)
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DEPs coincident with the tissue expression. (A) Numbers of DEPs coincident with the tissue expression. (B) DEPs which were mainly expressed by enterocytes in single-cell RNA data of gastric tissue samples. (C) DEPs with high expression specificity in enterocyte in Human Protein Atlas data. (D) <t>APOA4</t> levels in the gastric tissue samples. The barplot is the statistical result of the optical density analysis of IHC images. These tissue samples include NAG ( n = 10), IM ( n = 16), and GC ( n = 16). * p < 0.05; ** p < 0.01. (E) DEPs which were mainly expressed by epithelial cells in single-cell RNA data. (F) Fold changes of DEPs across cell types in pre-GC vs gastritis/normal comparison group. (G) DEPs coincident with the RNA datasets, GSE55696 and GSE130823. (H–J) ROC curves of the random forest models using the signature, APOA4, SERPINA4, and GCA.
Gr3379814 4 Apoa4 Proteintech 17996 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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17996 1 ap  (Proteintech)
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DEPs coincident with the tissue expression. (A) Numbers of DEPs coincident with the tissue expression. (B) DEPs which were mainly expressed by enterocytes in single-cell RNA data of gastric tissue samples. (C) DEPs with high expression specificity in enterocyte in Human Protein Atlas data. (D) <t>APOA4</t> levels in the gastric tissue samples. The barplot is the statistical result of the optical density analysis of IHC images. These tissue samples include NAG ( n = 10), IM ( n = 16), and GC ( n = 16). * p < 0.05; ** p < 0.01. (E) DEPs which were mainly expressed by epithelial cells in single-cell RNA data. (F) Fold changes of DEPs across cell types in pre-GC vs gastritis/normal comparison group. (G) DEPs coincident with the RNA datasets, GSE55696 and GSE130823. (H–J) ROC curves of the random forest models using the signature, APOA4, SERPINA4, and GCA.
17996 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti apoa4  (Proteintech)
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Expression patterns of transporter genes and validation by immunofluorescence or immunohistochemistry. (A) Expression patterns of eight selected transporter genes in different intestine segments. Each dot represents a gene, of which the color saturation indicates the average expression level (scaled by Z-score) in an intestine segment and the size indicates the percentage of cells expressing the gene. (B, C, and E–G) Immunofluorescence was performed to confirm the expression of APOB (B) and FABP6 (D) in the ileum and SLC38A1 (E), SLC26A2 (F), and SLC44A1 (G) in the colon and rectum. (C, H, and I) Immunohistochemistry was performed to confirm the expression of <t>APOA4</t> in the ileum (C) and SCNN1B (H) and SLC35A1 (I) in the colon and rectum. Scale bars, 100 μm.
Rabbit Anti Apoa4, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DEPs coincident with the tissue expression. (A) Numbers of DEPs coincident with the tissue expression. (B) DEPs which were mainly expressed by enterocytes in single-cell RNA data of gastric tissue samples. (C) DEPs with high expression specificity in enterocyte in Human Protein Atlas data. (D) APOA4 levels in the gastric tissue samples. The barplot is the statistical result of the optical density analysis of IHC images. These tissue samples include NAG ( n = 10), IM ( n = 16), and GC ( n = 16). * p < 0.05; ** p < 0.01. (E) DEPs which were mainly expressed by epithelial cells in single-cell RNA data. (F) Fold changes of DEPs across cell types in pre-GC vs gastritis/normal comparison group. (G) DEPs coincident with the RNA datasets, GSE55696 and GSE130823. (H–J) ROC curves of the random forest models using the signature, APOA4, SERPINA4, and GCA.

Journal: Frontiers in Molecular Biosciences

Article Title: Serum proteomic profiling of precancerous gastric lesions and early gastric cancer reveals signatures associated with systemic inflammatory response and metaplastic differentiation

doi: 10.3389/fmolb.2024.1252058

Figure Lengend Snippet: DEPs coincident with the tissue expression. (A) Numbers of DEPs coincident with the tissue expression. (B) DEPs which were mainly expressed by enterocytes in single-cell RNA data of gastric tissue samples. (C) DEPs with high expression specificity in enterocyte in Human Protein Atlas data. (D) APOA4 levels in the gastric tissue samples. The barplot is the statistical result of the optical density analysis of IHC images. These tissue samples include NAG ( n = 10), IM ( n = 16), and GC ( n = 16). * p < 0.05; ** p < 0.01. (E) DEPs which were mainly expressed by epithelial cells in single-cell RNA data. (F) Fold changes of DEPs across cell types in pre-GC vs gastritis/normal comparison group. (G) DEPs coincident with the RNA datasets, GSE55696 and GSE130823. (H–J) ROC curves of the random forest models using the signature, APOA4, SERPINA4, and GCA.

Article Snippet: APOA4 antibody (17996-1-AP, Proteintech, USA) and COMP antibody (28369-1-AP, Proteintech, USA) were diluted at ratio 1:200.

Techniques: Expressing, Comparison

Expression patterns of transporter genes and validation by immunofluorescence or immunohistochemistry. (A) Expression patterns of eight selected transporter genes in different intestine segments. Each dot represents a gene, of which the color saturation indicates the average expression level (scaled by Z-score) in an intestine segment and the size indicates the percentage of cells expressing the gene. (B, C, and E–G) Immunofluorescence was performed to confirm the expression of APOB (B) and FABP6 (D) in the ileum and SLC38A1 (E), SLC26A2 (F), and SLC44A1 (G) in the colon and rectum. (C, H, and I) Immunohistochemistry was performed to confirm the expression of APOA4 in the ileum (C) and SCNN1B (H) and SLC35A1 (I) in the colon and rectum. Scale bars, 100 μm.

Journal: The Journal of Experimental Medicine

Article Title: Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine

doi: 10.1084/jem.20191130

Figure Lengend Snippet: Expression patterns of transporter genes and validation by immunofluorescence or immunohistochemistry. (A) Expression patterns of eight selected transporter genes in different intestine segments. Each dot represents a gene, of which the color saturation indicates the average expression level (scaled by Z-score) in an intestine segment and the size indicates the percentage of cells expressing the gene. (B, C, and E–G) Immunofluorescence was performed to confirm the expression of APOB (B) and FABP6 (D) in the ileum and SLC38A1 (E), SLC26A2 (F), and SLC44A1 (G) in the colon and rectum. (C, H, and I) Immunohistochemistry was performed to confirm the expression of APOA4 in the ileum (C) and SCNN1B (H) and SLC35A1 (I) in the colon and rectum. Scale bars, 100 μm.

Article Snippet: Rabbit anti-LYZ (1:200, ab108508; Abcam), mouse anti-MUC2 (1:200, ab11197; Abcam), rabbit anti-NUSAP1 (1:100, 12024–1-AP; Proteintech), mouse-anti Ki67 (1:300, 9449s; CST), mouse anti-E-Cad (1:1,000, 610182; BD Biosciences), rabbit anti-ITLN1 (1:50, 11770–1-AP; Proteintech), rabbit anti-TFF1 (1:50, 13734–1-AP; Proteintech), rabbit anti-APOB (1:50, 20578–1-AP; Proteintech), rabbit anti-APOA4 (1:100, 17996–1-AP; Proteintech), rabbit anti-SLC26A2 (1:200, 27759–1-AP; Proteintech), rabbit anti-SCNN1B (1:200, 14134–1-AP; Proteintech), rabbit anti-SLC35A1 (1:400, 16342–1-AP; Proteintech), rabbit anti-FABP6 (1:500, 13781–1-AP; Proteintech), rabbit anti-SLC38A1 (1:100, 12039–1-AP; Proteintech), and rabbit anti-SLC44A1 (1:100, 14687–1-AP; Proteintech).

Techniques: Expressing, Immunofluorescence, Immunohistochemistry