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Astrocyte enriched genes across injury microenvironments 30 days post injury. A Scatter plots show the top 500 genes enriched in astrocytes as defined by (Zhang et al. ) in the 3 mm of spinal cord at the SCI Epicenter, Above, or Below relative to that in the uninjured spinal cord. Table lists the 15 astrocyte enriched genes that were most highly up or downregulated after SCI in terms of fold change (F.C.) (Table provides full list of enriched genes by region). B Heat map shows differential expression of select genes associated with all astrocytes or those with a pro-inflammatory (A1) or pre-repair (A2) phenotype at 30 days post SCI with minimum and maximum expression levels in each case scaled across the 3 injury zones and an equivalent region of uninjured spinal cord (n = 4 female mice for uninjured controls (UI) and for SCI). Asterisks on the heatmaps represents DEGs (|Log2.FC|> 1.0 and FDR < 0.05). C Representative photomicrographs (uninjured and SCI Epicenter only) and associated histograms show the abundance of immunofluorescence staining for a pan marker of astrocyte activation (GFAP), or for makers of the pro-inflammatory (C3D, Serping1) or pro-repair (Emp1, <t>S100A10)</t> phenotypes across the gray matter (GM) and white matter (WM) of the ventrolateral spinal cord within the uninjured spinal cord or in a 3 mm segment spanning the SCI Epicenter (E), the Above (A), or the Below (B) regions 32 dpi (GFAP, One-Way ANOVA, SNK, p = 0.03; C3D, One-Way ANOVA, Dunn’s, p = 0.03, S100A10, One-Way ANOVA, Dunn’s, p = 0.048, n = 3 uninjured, n = 5–6 SCI). See Fig. for co-localization of each antigen. Scale bar = 100 μM
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Astrocyte enriched genes across injury microenvironments 30 days post injury. A Scatter plots show the top 500 genes enriched in astrocytes as defined by (Zhang et al. ) in the 3 mm of spinal cord at the SCI Epicenter, Above, or Below relative to that in the uninjured spinal cord. Table lists the 15 astrocyte enriched genes that were most highly up or downregulated after SCI in terms of fold change (F.C.) (Table provides full list of enriched genes by region). B Heat map shows differential expression of select genes associated with all astrocytes or those with a pro-inflammatory (A1) or pre-repair (A2) phenotype at 30 days post SCI with minimum and maximum expression levels in each case scaled across the 3 injury zones and an equivalent region of uninjured spinal cord (n = 4 female mice for uninjured controls (UI) and for SCI). Asterisks on the heatmaps represents DEGs (|Log2.FC|> 1.0 and FDR < 0.05). C Representative photomicrographs (uninjured and SCI Epicenter only) and associated histograms show the abundance of immunofluorescence staining for a pan marker of astrocyte activation (GFAP), or for makers of the pro-inflammatory (C3D, Serping1) or pro-repair (Emp1, <t>S100A10)</t> phenotypes across the gray matter (GM) and white matter (WM) of the ventrolateral spinal cord within the uninjured spinal cord or in a 3 mm segment spanning the SCI Epicenter (E), the Above (A), or the Below (B) regions 32 dpi (GFAP, One-Way ANOVA, SNK, p = 0.03; C3D, One-Way ANOVA, Dunn’s, p = 0.03, S100A10, One-Way ANOVA, Dunn’s, p = 0.048, n = 3 uninjured, n = 5–6 SCI). See Fig. for co-localization of each antigen. Scale bar = 100 μM
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Astrocyte enriched genes across injury microenvironments 30 days post injury. A Scatter plots show the top 500 genes enriched in astrocytes as defined by (Zhang et al. ) in the 3 mm of spinal cord at the SCI Epicenter, Above, or Below relative to that in the uninjured spinal cord. Table lists the 15 astrocyte enriched genes that were most highly up or downregulated after SCI in terms of fold change (F.C.) (Table provides full list of enriched genes by region). B Heat map shows differential expression of select genes associated with all astrocytes or those with a pro-inflammatory (A1) or pre-repair (A2) phenotype at 30 days post SCI with minimum and maximum expression levels in each case scaled across the 3 injury zones and an equivalent region of uninjured spinal cord (n = 4 female mice for uninjured controls (UI) and for SCI). Asterisks on the heatmaps represents DEGs (|Log2.FC|> 1.0 and FDR < 0.05). C Representative photomicrographs (uninjured and SCI Epicenter only) and associated histograms show the abundance of immunofluorescence staining for a pan marker of astrocyte activation (GFAP), or for makers of the pro-inflammatory (C3D, Serping1) or pro-repair (Emp1, <t>S100A10)</t> phenotypes across the gray matter (GM) and white matter (WM) of the ventrolateral spinal cord within the uninjured spinal cord or in a 3 mm segment spanning the SCI Epicenter (E), the Above (A), or the Below (B) regions 32 dpi (GFAP, One-Way ANOVA, SNK, p = 0.03; C3D, One-Way ANOVA, Dunn’s, p = 0.03, S100A10, One-Way ANOVA, Dunn’s, p = 0.048, n = 3 uninjured, n = 5–6 SCI). See Fig. for co-localization of each antigen. Scale bar = 100 μM
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Astrocyte enriched genes across injury microenvironments 30 days post injury. A Scatter plots show the top 500 genes enriched in astrocytes as defined by (Zhang et al. ) in the 3 mm of spinal cord at the SCI Epicenter, Above, or Below relative to that in the uninjured spinal cord. Table lists the 15 astrocyte enriched genes that were most highly up or downregulated after SCI in terms of fold change (F.C.) (Table provides full list of enriched genes by region). B Heat map shows differential expression of select genes associated with all astrocytes or those with a pro-inflammatory (A1) or pre-repair (A2) phenotype at 30 days post SCI with minimum and maximum expression levels in each case scaled across the 3 injury zones and an equivalent region of uninjured spinal cord (n = 4 female mice for uninjured controls (UI) and for SCI). Asterisks on the heatmaps represents DEGs (|Log2.FC|> 1.0 and FDR < 0.05). C Representative photomicrographs (uninjured and SCI Epicenter only) and associated histograms show the abundance of immunofluorescence staining for a pan marker of astrocyte activation (GFAP), or for makers of the pro-inflammatory (C3D, Serping1) or pro-repair (Emp1, <t>S100A10)</t> phenotypes across the gray matter (GM) and white matter (WM) of the ventrolateral spinal cord within the uninjured spinal cord or in a 3 mm segment spanning the SCI Epicenter (E), the Above (A), or the Below (B) regions 32 dpi (GFAP, One-Way ANOVA, SNK, p = 0.03; C3D, One-Way ANOVA, Dunn’s, p = 0.03, S100A10, One-Way ANOVA, Dunn’s, p = 0.048, n = 3 uninjured, n = 5–6 SCI). See Fig. for co-localization of each antigen. Scale bar = 100 μM
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Image Search Results


Astrocyte enriched genes across injury microenvironments 30 days post injury. A Scatter plots show the top 500 genes enriched in astrocytes as defined by (Zhang et al. ) in the 3 mm of spinal cord at the SCI Epicenter, Above, or Below relative to that in the uninjured spinal cord. Table lists the 15 astrocyte enriched genes that were most highly up or downregulated after SCI in terms of fold change (F.C.) (Table provides full list of enriched genes by region). B Heat map shows differential expression of select genes associated with all astrocytes or those with a pro-inflammatory (A1) or pre-repair (A2) phenotype at 30 days post SCI with minimum and maximum expression levels in each case scaled across the 3 injury zones and an equivalent region of uninjured spinal cord (n = 4 female mice for uninjured controls (UI) and for SCI). Asterisks on the heatmaps represents DEGs (|Log2.FC|> 1.0 and FDR < 0.05). C Representative photomicrographs (uninjured and SCI Epicenter only) and associated histograms show the abundance of immunofluorescence staining for a pan marker of astrocyte activation (GFAP), or for makers of the pro-inflammatory (C3D, Serping1) or pro-repair (Emp1, S100A10) phenotypes across the gray matter (GM) and white matter (WM) of the ventrolateral spinal cord within the uninjured spinal cord or in a 3 mm segment spanning the SCI Epicenter (E), the Above (A), or the Below (B) regions 32 dpi (GFAP, One-Way ANOVA, SNK, p = 0.03; C3D, One-Way ANOVA, Dunn’s, p = 0.03, S100A10, One-Way ANOVA, Dunn’s, p = 0.048, n = 3 uninjured, n = 5–6 SCI). See Fig. for co-localization of each antigen. Scale bar = 100 μM

Journal: Cellular and Molecular Neurobiology

Article Title: Regional Molecular Diversity in Chronic Spinal Cord Injury

doi: 10.1007/s10571-026-01694-x

Figure Lengend Snippet: Astrocyte enriched genes across injury microenvironments 30 days post injury. A Scatter plots show the top 500 genes enriched in astrocytes as defined by (Zhang et al. ) in the 3 mm of spinal cord at the SCI Epicenter, Above, or Below relative to that in the uninjured spinal cord. Table lists the 15 astrocyte enriched genes that were most highly up or downregulated after SCI in terms of fold change (F.C.) (Table provides full list of enriched genes by region). B Heat map shows differential expression of select genes associated with all astrocytes or those with a pro-inflammatory (A1) or pre-repair (A2) phenotype at 30 days post SCI with minimum and maximum expression levels in each case scaled across the 3 injury zones and an equivalent region of uninjured spinal cord (n = 4 female mice for uninjured controls (UI) and for SCI). Asterisks on the heatmaps represents DEGs (|Log2.FC|> 1.0 and FDR < 0.05). C Representative photomicrographs (uninjured and SCI Epicenter only) and associated histograms show the abundance of immunofluorescence staining for a pan marker of astrocyte activation (GFAP), or for makers of the pro-inflammatory (C3D, Serping1) or pro-repair (Emp1, S100A10) phenotypes across the gray matter (GM) and white matter (WM) of the ventrolateral spinal cord within the uninjured spinal cord or in a 3 mm segment spanning the SCI Epicenter (E), the Above (A), or the Below (B) regions 32 dpi (GFAP, One-Way ANOVA, SNK, p = 0.03; C3D, One-Way ANOVA, Dunn’s, p = 0.03, S100A10, One-Way ANOVA, Dunn’s, p = 0.048, n = 3 uninjured, n = 5–6 SCI). See Fig. for co-localization of each antigen. Scale bar = 100 μM

Article Snippet: S100A10 , Rabbit , Protein Tech (11250-1-AP) , AB_2269906 , 1:7500.

Techniques: Quantitative Proteomics, Expressing, Immunofluorescence, Staining, Marker, Activation Assay