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ImP alters gene expression in the murine colon and inhibits the PPAR‐γ signaling pathway. (A) Principle component analysis (PCA) of transcriptional profiling. (B) Volcano plot for the differential gene between normal and ImP‐treated mice. (C) Significantly changed pathways analysised by Kyoto Encyclopedia of Genes and Genomes (KEGG). (D) Gene set enrichment analysis (GSEA) of PPAR‐γ signaling between the normal and ImP groups. (E) Different expressed genes in the PPAR signaling pathway between Ctrl group and ImP group. (F) Search tool for recurring instances of neighboring genes (STRING) network in the PPAR signaling pathway. (G) mRNA level of the differentially expressed genes in the PPAR signaling pathway. (H) Immunofluorescent analysis in colonic sections. PPAR‐γ (red). Nuclei (blue); scale bar = 100 μm. (I) Immunoblot analysis of PPAR‐γ and <t>APOA1</t> in colonic tissue. (J) NCM460 cells were treated with ImP for 12 h, and cell viability analysis was detected by a CCK‐8 assay. (J, K) NCM460 cells were incubated with ImP for 12 h, and the expression of tight junction genes and the PPARγ signaling pathway was examined by real‐time PCR assay. (L) Immunoblot analysis of PPAR‐γ in NCM460 cells. Data are denoted as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.
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ImP alters gene expression in the murine colon and inhibits the PPAR‐γ signaling pathway. (A) Principle component analysis (PCA) of transcriptional profiling. (B) Volcano plot for the differential gene between normal and ImP‐treated mice. (C) Significantly changed pathways analysised by Kyoto Encyclopedia of Genes and Genomes (KEGG). (D) Gene set enrichment analysis (GSEA) of PPAR‐γ signaling between the normal and ImP groups. (E) Different expressed genes in the PPAR signaling pathway between Ctrl group and ImP group. (F) Search tool for recurring instances of neighboring genes (STRING) network in the PPAR signaling pathway. (G) mRNA level of the differentially expressed genes in the PPAR signaling pathway. (H) Immunofluorescent analysis in colonic sections. PPAR‐γ (red). Nuclei (blue); scale bar = 100 μm. (I) Immunoblot analysis of PPAR‐γ and <t>APOA1</t> in colonic tissue. (J) NCM460 cells were treated with ImP for 12 h, and cell viability analysis was detected by a CCK‐8 assay. (J, K) NCM460 cells were incubated with ImP for 12 h, and the expression of tight junction genes and the PPARγ signaling pathway was examined by real‐time PCR assay. (L) Immunoblot analysis of PPAR‐γ in NCM460 cells. Data are denoted as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.
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ImP alters gene expression in the murine colon and inhibits the PPAR‐γ signaling pathway. (A) Principle component analysis (PCA) of transcriptional profiling. (B) Volcano plot for the differential gene between normal and ImP‐treated mice. (C) Significantly changed pathways analysised by Kyoto Encyclopedia of Genes and Genomes (KEGG). (D) Gene set enrichment analysis (GSEA) of PPAR‐γ signaling between the normal and ImP groups. (E) Different expressed genes in the PPAR signaling pathway between Ctrl group and ImP group. (F) Search tool for recurring instances of neighboring genes (STRING) network in the PPAR signaling pathway. (G) mRNA level of the differentially expressed genes in the PPAR signaling pathway. (H) Immunofluorescent analysis in colonic sections. PPAR‐γ (red). Nuclei (blue); scale bar = 100 μm. (I) Immunoblot analysis of PPAR‐γ and <t>APOA1</t> in colonic tissue. (J) NCM460 cells were treated with ImP for 12 h, and cell viability analysis was detected by a CCK‐8 assay. (J, K) NCM460 cells were incubated with ImP for 12 h, and the expression of tight junction genes and the PPARγ signaling pathway was examined by real‐time PCR assay. (L) Immunoblot analysis of PPAR‐γ in NCM460 cells. Data are denoted as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.
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ImP alters gene expression in the murine colon and inhibits the PPAR‐γ signaling pathway. (A) Principle component analysis (PCA) of transcriptional profiling. (B) Volcano plot for the differential gene between normal and ImP‐treated mice. (C) Significantly changed pathways analysised by Kyoto Encyclopedia of Genes and Genomes (KEGG). (D) Gene set enrichment analysis (GSEA) of PPAR‐γ signaling between the normal and ImP groups. (E) Different expressed genes in the PPAR signaling pathway between Ctrl group and ImP group. (F) Search tool for recurring instances of neighboring genes (STRING) network in the PPAR signaling pathway. (G) mRNA level of the differentially expressed genes in the PPAR signaling pathway. (H) Immunofluorescent analysis in colonic sections. PPAR‐γ (red). Nuclei (blue); scale bar = 100 μm. (I) Immunoblot analysis of PPAR‐γ and <t>APOA1</t> in colonic tissue. (J) NCM460 cells were treated with ImP for 12 h, and cell viability analysis was detected by a CCK‐8 assay. (J, K) NCM460 cells were incubated with ImP for 12 h, and the expression of tight junction genes and the PPARγ signaling pathway was examined by real‐time PCR assay. (L) Immunoblot analysis of PPAR‐γ in NCM460 cells. Data are denoted as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.
Tbst, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ImP alters gene expression in the murine colon and inhibits the PPAR‐γ signaling pathway. (A) Principle component analysis (PCA) of transcriptional profiling. (B) Volcano plot for the differential gene between normal and ImP‐treated mice. (C) Significantly changed pathways analysised by Kyoto Encyclopedia of Genes and Genomes (KEGG). (D) Gene set enrichment analysis (GSEA) of PPAR‐γ signaling between the normal and ImP groups. (E) Different expressed genes in the PPAR signaling pathway between Ctrl group and ImP group. (F) Search tool for recurring instances of neighboring genes (STRING) network in the PPAR signaling pathway. (G) mRNA level of the differentially expressed genes in the PPAR signaling pathway. (H) Immunofluorescent analysis in colonic sections. PPAR‐γ (red). Nuclei (blue); scale bar = 100 μm. (I) Immunoblot analysis of PPAR‐γ and <t>APOA1</t> in colonic tissue. (J) NCM460 cells were treated with ImP for 12 h, and cell viability analysis was detected by a CCK‐8 assay. (J, K) NCM460 cells were incubated with ImP for 12 h, and the expression of tight junction genes and the PPARγ signaling pathway was examined by real‐time PCR assay. (L) Immunoblot analysis of PPAR‐γ in NCM460 cells. Data are denoted as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.
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ImP alters gene expression in the murine colon and inhibits the PPAR‐γ signaling pathway. (A) Principle component analysis (PCA) of transcriptional profiling. (B) Volcano plot for the differential gene between normal and ImP‐treated mice. (C) Significantly changed pathways analysised by Kyoto Encyclopedia of Genes and Genomes (KEGG). (D) Gene set enrichment analysis (GSEA) of PPAR‐γ signaling between the normal and ImP groups. (E) Different expressed genes in the PPAR signaling pathway between Ctrl group and ImP group. (F) Search tool for recurring instances of neighboring genes (STRING) network in the PPAR signaling pathway. (G) mRNA level of the differentially expressed genes in the PPAR signaling pathway. (H) Immunofluorescent analysis in colonic sections. PPAR‐γ (red). Nuclei (blue); scale bar = 100 μm. (I) Immunoblot analysis of PPAR‐γ and <t>APOA1</t> in colonic tissue. (J) NCM460 cells were treated with ImP for 12 h, and cell viability analysis was detected by a CCK‐8 assay. (J, K) NCM460 cells were incubated with ImP for 12 h, and the expression of tight junction genes and the PPARγ signaling pathway was examined by real‐time PCR assay. (L) Immunoblot analysis of PPAR‐γ in NCM460 cells. Data are denoted as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.
Apoai, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ImP alters gene expression in the murine colon and inhibits the PPAR‐γ signaling pathway. (A) Principle component analysis (PCA) of transcriptional profiling. (B) Volcano plot for the differential gene between normal and ImP‐treated mice. (C) Significantly changed pathways analysised by Kyoto Encyclopedia of Genes and Genomes (KEGG). (D) Gene set enrichment analysis (GSEA) of PPAR‐γ signaling between the normal and ImP groups. (E) Different expressed genes in the PPAR signaling pathway between Ctrl group and ImP group. (F) Search tool for recurring instances of neighboring genes (STRING) network in the PPAR signaling pathway. (G) mRNA level of the differentially expressed genes in the PPAR signaling pathway. (H) Immunofluorescent analysis in colonic sections. PPAR‐γ (red). Nuclei (blue); scale bar = 100 μm. (I) Immunoblot analysis of PPAR‐γ and <t>APOA1</t> in colonic tissue. (J) NCM460 cells were treated with ImP for 12 h, and cell viability analysis was detected by a CCK‐8 assay. (J, K) NCM460 cells were incubated with ImP for 12 h, and the expression of tight junction genes and the PPARγ signaling pathway was examined by real‐time PCR assay. (L) Immunoblot analysis of PPAR‐γ in NCM460 cells. Data are denoted as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.
Apoa1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ImP alters gene expression in the murine colon and inhibits the PPAR‐γ signaling pathway. (A) Principle component analysis (PCA) of transcriptional profiling. (B) Volcano plot for the differential gene between normal and ImP‐treated mice. (C) Significantly changed pathways analysised by Kyoto Encyclopedia of Genes and Genomes (KEGG). (D) Gene set enrichment analysis (GSEA) of PPAR‐γ signaling between the normal and ImP groups. (E) Different expressed genes in the PPAR signaling pathway between Ctrl group and ImP group. (F) Search tool for recurring instances of neighboring genes (STRING) network in the PPAR signaling pathway. (G) mRNA level of the differentially expressed genes in the PPAR signaling pathway. (H) Immunofluorescent analysis in colonic sections. PPAR‐γ (red). Nuclei (blue); scale bar = 100 μm. (I) Immunoblot analysis of PPAR‐γ and APOA1 in colonic tissue. (J) NCM460 cells were treated with ImP for 12 h, and cell viability analysis was detected by a CCK‐8 assay. (J, K) NCM460 cells were incubated with ImP for 12 h, and the expression of tight junction genes and the PPARγ signaling pathway was examined by real‐time PCR assay. (L) Immunoblot analysis of PPAR‐γ in NCM460 cells. Data are denoted as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Journal: Phytotherapy Research

Article Title: Angelica dahurica Polysaccharides Ameliorate Colitis by Reducing the Restriction of Gut Microbiota‐Derived Imidazole Propionate on PPAR ‐γ Signaling Activation

doi: 10.1002/ptr.8466

Figure Lengend Snippet: ImP alters gene expression in the murine colon and inhibits the PPAR‐γ signaling pathway. (A) Principle component analysis (PCA) of transcriptional profiling. (B) Volcano plot for the differential gene between normal and ImP‐treated mice. (C) Significantly changed pathways analysised by Kyoto Encyclopedia of Genes and Genomes (KEGG). (D) Gene set enrichment analysis (GSEA) of PPAR‐γ signaling between the normal and ImP groups. (E) Different expressed genes in the PPAR signaling pathway between Ctrl group and ImP group. (F) Search tool for recurring instances of neighboring genes (STRING) network in the PPAR signaling pathway. (G) mRNA level of the differentially expressed genes in the PPAR signaling pathway. (H) Immunofluorescent analysis in colonic sections. PPAR‐γ (red). Nuclei (blue); scale bar = 100 μm. (I) Immunoblot analysis of PPAR‐γ and APOA1 in colonic tissue. (J) NCM460 cells were treated with ImP for 12 h, and cell viability analysis was detected by a CCK‐8 assay. (J, K) NCM460 cells were incubated with ImP for 12 h, and the expression of tight junction genes and the PPARγ signaling pathway was examined by real‐time PCR assay. (L) Immunoblot analysis of PPAR‐γ in NCM460 cells. Data are denoted as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: FITC‐dextran (4 kDa; average MW 3000–5000, Cat# 46944, CAS: 60842–46‐8) was bought from Sigma‐Aldrich (St. Louis, MO, USA). β‐actin (Cat# 66009–1‐Ig), HRP‐conjugated Goat‐Anti‐Mouse IgG (Cat# SA00001–1), HRP‐conjugated Goat Anti‐Rabbit IgG (Cat# SA00001–2), and Apolipoprotein AI Polyclonal antibody (ApoA1, Cat# 14427‐1‐AP) were bought from Proteintech Group (Rosemont, USA).

Techniques: Gene Expression, Western Blot, CCK-8 Assay, Incubation, Expressing, Real-time Polymerase Chain Reaction