treponema denticola atcc 35 405  (ATCC)


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    ATCC treponema denticola atcc 35 405
    Boxplots demonstrating cell counts per endodontic-like biofilm on pellicle-coated hydroxyapatite discs after analysis by qPCR. To form endodontic-like multispecies biofilms, a total of six bacterial species were added separately to a “basic” nine-species subgingival biofilm. x -axis of panal ( A ) shows the strains of the “basic biofilm” (in the first column total counts (beige) as a control group are shown), while x -axis of panal ( B ) shows in the first column total counts (beige) again, as well as the strains of the endodontic species ( E. faecalis (blue), S. aureus (dark green), P. nigrescens (red), S. sputigena (orange), P. micra (light green) and T. denticola (pink)). Statistically significant differences between the biofilm with additional strains and the control group (“basic biofilm” or endodontic-like biofilm) is marked with 1–4 asterisks (* p < 0.05; ** p < 0.01; *** p < 0.001). The internal line represents the median; the whiskers indicate minimum and maximum. The p values ( p ≤ 0.05) of the significantly different data are provided. Data derive from three independent experiments, each represented in triplicate biofilm cultures ( n = 9).
    Treponema Denticola Atcc 35 405, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Endodontic-Like Oral Biofilms as Models for Multispecies Interactions in Endodontic Diseases"

    Article Title: Endodontic-Like Oral Biofilms as Models for Multispecies Interactions in Endodontic Diseases

    Journal: Microorganisms

    doi: 10.3390/microorganisms8050674

    Boxplots demonstrating cell counts per endodontic-like biofilm on pellicle-coated hydroxyapatite discs after analysis by qPCR. To form endodontic-like multispecies biofilms, a total of six bacterial species were added separately to a “basic” nine-species subgingival biofilm. x -axis of panal ( A ) shows the strains of the “basic biofilm” (in the first column total counts (beige) as a control group are shown), while x -axis of panal ( B ) shows in the first column total counts (beige) again, as well as the strains of the endodontic species ( E. faecalis (blue), S. aureus (dark green), P. nigrescens (red), S. sputigena (orange), P. micra (light green) and T. denticola (pink)). Statistically significant differences between the biofilm with additional strains and the control group (“basic biofilm” or endodontic-like biofilm) is marked with 1–4 asterisks (* p < 0.05; ** p < 0.01; *** p < 0.001). The internal line represents the median; the whiskers indicate minimum and maximum. The p values ( p ≤ 0.05) of the significantly different data are provided. Data derive from three independent experiments, each represented in triplicate biofilm cultures ( n = 9).
    Figure Legend Snippet: Boxplots demonstrating cell counts per endodontic-like biofilm on pellicle-coated hydroxyapatite discs after analysis by qPCR. To form endodontic-like multispecies biofilms, a total of six bacterial species were added separately to a “basic” nine-species subgingival biofilm. x -axis of panal ( A ) shows the strains of the “basic biofilm” (in the first column total counts (beige) as a control group are shown), while x -axis of panal ( B ) shows in the first column total counts (beige) again, as well as the strains of the endodontic species ( E. faecalis (blue), S. aureus (dark green), P. nigrescens (red), S. sputigena (orange), P. micra (light green) and T. denticola (pink)). Statistically significant differences between the biofilm with additional strains and the control group (“basic biofilm” or endodontic-like biofilm) is marked with 1–4 asterisks (* p < 0.05; ** p < 0.01; *** p < 0.001). The internal line represents the median; the whiskers indicate minimum and maximum. The p values ( p ≤ 0.05) of the significantly different data are provided. Data derive from three independent experiments, each represented in triplicate biofilm cultures ( n = 9).

    Techniques Used:

    Boxplots demonstrating cell counts per endodontic-like biofilm on dentin discs after analysis by qPCR. To form endodontic-like multispecies biofilms, a total of six bacterial species were added separately to a “basic” nine-species subgingival biofilm. The x -axis of panal ( A ) shows the strains of the “basic biofilm” (in the first column total counts (beige) as a control group are shown), while the x-axis of panal ( B ) shows in the first column total counts (beige) again, as well as the strains of the endodontic species ( E. faecalis (blue), S. aureus (dark green), P. nigrescens (red), S. sputigena (orange), P. micra (light green) and T. denticola (pink)). Statistically significant differences between the biofilm with additional strains and the control group (basic biofilm or all species biofilm) is marked with 1–4 asterisks (* p < 0.05; ** p < 0.01; *** p < 0.001). The internal line represents the median; whiskers indicate minimum and maximum. The p values ( p ≤ 0.05) of the significantly different data are provided. The data were derived from three independent experiments, each represented in triplicate biofilm cultures ( n = 9).
    Figure Legend Snippet: Boxplots demonstrating cell counts per endodontic-like biofilm on dentin discs after analysis by qPCR. To form endodontic-like multispecies biofilms, a total of six bacterial species were added separately to a “basic” nine-species subgingival biofilm. The x -axis of panal ( A ) shows the strains of the “basic biofilm” (in the first column total counts (beige) as a control group are shown), while the x-axis of panal ( B ) shows in the first column total counts (beige) again, as well as the strains of the endodontic species ( E. faecalis (blue), S. aureus (dark green), P. nigrescens (red), S. sputigena (orange), P. micra (light green) and T. denticola (pink)). Statistically significant differences between the biofilm with additional strains and the control group (basic biofilm or all species biofilm) is marked with 1–4 asterisks (* p < 0.05; ** p < 0.01; *** p < 0.001). The internal line represents the median; whiskers indicate minimum and maximum. The p values ( p ≤ 0.05) of the significantly different data are provided. The data were derived from three independent experiments, each represented in triplicate biofilm cultures ( n = 9).

    Techniques Used: Derivative Assay

    Confocal laser scanning microscopy (CLSM) 3D reconstructions and image stacks (insets) of endodontic-like biofilms grown on HA discs following fluorescence in situ hybridization (FISH) using FITC- and Cy3-labelled probes (see  ). To form endodontic-like multispecies biofilms, a total of six bacterial species were added separately to a “basic” nine-species subgingival biofilm. The resulting biofilms 1–6 contained additionally E. faecalis ( A ), or S. aureus ( B ), or P. nigrescens ( C ), or P. micra ( D ), or S. sputigena ( E ), or T. denticola ( F ). Prevotella intermedia appears green (FITC-labeled) and the newly added bacteria appear red (Cy3-labeled). Non-hybridized bacteria appear blue due to DNA staining (YoPro 59). Scale bar = 10 µm.
    Figure Legend Snippet: Confocal laser scanning microscopy (CLSM) 3D reconstructions and image stacks (insets) of endodontic-like biofilms grown on HA discs following fluorescence in situ hybridization (FISH) using FITC- and Cy3-labelled probes (see ). To form endodontic-like multispecies biofilms, a total of six bacterial species were added separately to a “basic” nine-species subgingival biofilm. The resulting biofilms 1–6 contained additionally E. faecalis ( A ), or S. aureus ( B ), or P. nigrescens ( C ), or P. micra ( D ), or S. sputigena ( E ), or T. denticola ( F ). Prevotella intermedia appears green (FITC-labeled) and the newly added bacteria appear red (Cy3-labeled). Non-hybridized bacteria appear blue due to DNA staining (YoPro 59). Scale bar = 10 µm.

    Techniques Used: Confocal Laser Scanning Microscopy, Fluorescence, In Situ Hybridization, Labeling, Staining

    Confocal laser scanning microscopy (CLSM) 3D reconstructions of the endodontic-like 15-species biofilms grown on HA discs following fluorescence in situ hybridization (FISH). To form endodontic-like multispecies biofilms, a total of six bacterial species were added to a “basic” nine-species subgingival biofilm. The resulting endodontic-like 15-species biofilms contained F. nucleatum (light blue; FITC-labeled) and P. intermedia (red; Cy3-labeled) ( A , B ); E. faecalis (green; FITC-labeled), P. micra (red; Cy3-labeled), S. aureus (yellow; ROX-labeled) ( C , D ); P. nigrescens (green; FITC-labeled), S. sputigena (red; Cy3-labeled), T. denticola (white; ROX-labeled) ( E , F ); P. gingivalis (green; FITC-labeled), T. forsythia (red; Cy3-labeled), P. intermedia (purple; ROX-labeled), F. nucleatum and C. rectus (blue; Cy5-labeled) ( G , H ). In images ( A – F ), non-hybridized bacteria appear blue due to DNA staining (YoPro 59). Scale bar = 10 µm ( A – E , G ) or 20 µm ( F , H ).
    Figure Legend Snippet: Confocal laser scanning microscopy (CLSM) 3D reconstructions of the endodontic-like 15-species biofilms grown on HA discs following fluorescence in situ hybridization (FISH). To form endodontic-like multispecies biofilms, a total of six bacterial species were added to a “basic” nine-species subgingival biofilm. The resulting endodontic-like 15-species biofilms contained F. nucleatum (light blue; FITC-labeled) and P. intermedia (red; Cy3-labeled) ( A , B ); E. faecalis (green; FITC-labeled), P. micra (red; Cy3-labeled), S. aureus (yellow; ROX-labeled) ( C , D ); P. nigrescens (green; FITC-labeled), S. sputigena (red; Cy3-labeled), T. denticola (white; ROX-labeled) ( E , F ); P. gingivalis (green; FITC-labeled), T. forsythia (red; Cy3-labeled), P. intermedia (purple; ROX-labeled), F. nucleatum and C. rectus (blue; Cy5-labeled) ( G , H ). In images ( A – F ), non-hybridized bacteria appear blue due to DNA staining (YoPro 59). Scale bar = 10 µm ( A – E , G ) or 20 µm ( F , H ).

    Techniques Used: Confocal Laser Scanning Microscopy, Fluorescence, In Situ Hybridization, Labeling, Staining