Journal: Cell reports

Article Title: Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity

doi: 10.1016/j.celrep.2022.111775

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-AAT , Proteintech , 16382-1-AP; RRID: AB_10641185.

Techniques: Recombinant, Electron Microscopy, Lysis, Lactate Assay, Pyruvate Assay, Enzyme-linked Immunosorbent Assay, RNA Sequencing Assay, Sequencing, Mutagenesis, Software, Modification

Journal: Disease Markers

Article Title: Identification of Differentially Expressed Serum Proteins in Infectious Purpura Fulminans

doi: 10.1155/2014/698383

Figure Lengend Snippet: List of 27 potentially purpura fulminans-associated proteins (SpC PF/N ≥ 2 or ≤0.5) identified by HPLC-Chip/MS across the two biological groups.

Article Snippet: Rabbit anti-alpha-1-antitrypsin (SERPINA1, Proteintech Group Inc., USA) and anti-alpha-2-antiplasmin (SERPINF2, Proteintech Group Inc., USA) were primary antibodies for the immunodetection.

Techniques: Coagulation, Binding Assay, Transduction, Derivative Assay

Journal: Disease Markers

Article Title: Identification of Differentially Expressed Serum Proteins in Infectious Purpura Fulminans

doi: 10.1155/2014/698383

Figure Lengend Snippet: Validation by Western blot of two differentially expressed proteins SERPINA1 and SERPINF2. Serum samples from three healthy individuals (N), one PF patient (PF), and three burn sepsis patients (B) were used to perform WB. (a) and (c) Validation by same proteins WB and histograms visualize normalized mean relative-integrated-density ± standard deviation values. The significance of expression changes was performed by one-way ANOVA test: * P ≤ 0.05. (b) and (d) Validation by same volumes WB and histograms visualize normalized mean relative-integrated-density ± standard deviation values. The significance of expression changes was performed by one-way ANOVA test: * P ≤ 0.05.

Article Snippet: Rabbit anti-alpha-1-antitrypsin (SERPINA1, Proteintech Group Inc., USA) and anti-alpha-2-antiplasmin (SERPINF2, Proteintech Group Inc., USA) were primary antibodies for the immunodetection.

Techniques: Western Blot, Standard Deviation, Expressing

Journal: Nutrients

Article Title: New Mechanisms of Bromelain in Alleviating Non-Alcoholic Fatty Liver Disease-Induced Deregulation of Blood Coagulation

doi: 10.3390/nu14112329

Figure Lengend Snippet: Effect of bromelain on the expression of proteins involved in the intrinsic, extrinsic, and common pathways in the liver of HFD−fed mice.

Article Snippet: Rabbit antibodies against serpin family G member 1 (SERPING1, 12259-1-AP) and α1-antitrypsin (16382-1-AP) were purchased from Proteintech (Rosemont, IL, USA).

Techniques: Expressing, Coagulation, Activity Assay

Journal: Nutrients

Article Title: New Mechanisms of Bromelain in Alleviating Non-Alcoholic Fatty Liver Disease-Induced Deregulation of Blood Coagulation

doi: 10.3390/nu14112329

Figure Lengend Snippet: Effect of BL on the expression of coagulation factors in the liver of HFD-fed mice. Eight-week-old C57BL/6 mice were fed with HFD and administered phosphate-buffered saline (PBS) or BL (20 mg/kg/day) for 12 weeks. The livers were collected and subjected to Western blot analysis. ( A , B ) The protein levels of antithrombin III (ATIII), serpin family G member 1 (SERPING1), α1-antitrypsin, FIB α, FIB β, FIB γ, plasminogen, and β-actin. Data represent the mean ± SEM from 10 mice. * p < 0.05 vs. HFD alone group. Abbreviations: ATIII, antithrombin III; BL, bromelain; HFD, high-fed diet; SERPING1, serpin family G member 1.

Article Snippet: Rabbit antibodies against serpin family G member 1 (SERPING1, 12259-1-AP) and α1-antitrypsin (16382-1-AP) were purchased from Proteintech (Rosemont, IL, USA).

Techniques: Expressing, Coagulation, Western Blot

Journal: Nutrients

Article Title: New Mechanisms of Bromelain in Alleviating Non-Alcoholic Fatty Liver Disease-Induced Deregulation of Blood Coagulation

doi: 10.3390/nu14112329

Figure Lengend Snippet: Effect of BL on the expression of coagulation factors in the plasma of HFD-fed mice. Eight-week-old C57BL/6 mice were fed with HFD and administered PBS or BL (20 mg/kg/day) for 12 weeks. The plasma was collected and subjected to Western blot analysis or enzyme-linked immunoassay (ELISA) assay. ( A – F ) The protein levels of ATIII, SERPING1, α1-antitrypsin, and plasminogen. ( G ) The levels of FIB were assessed by an ELISA kit. Data represent the mean ± SEM from 10 mice. * p < 0.05 vs. HFD alone group. Abbreviations: ATIII, antithrombin III; BL, bromelain; HFD, high-fed diet; SERPING1, serpin family G member 1.

Article Snippet: Rabbit antibodies against serpin family G member 1 (SERPING1, 12259-1-AP) and α1-antitrypsin (16382-1-AP) were purchased from Proteintech (Rosemont, IL, USA).

Techniques: Expressing, Coagulation, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Nutrients

Article Title: New Mechanisms of Bromelain in Alleviating Non-Alcoholic Fatty Liver Disease-Induced Deregulation of Blood Coagulation

doi: 10.3390/nu14112329

Figure Lengend Snippet: Schematic illustration of the molecular mechanism through which bromelain induces anticoagulant effects in HFD−fed mice. As shown, daily administration of BL for 12 weeks increased the expression of ATIII, SERPING1, α1-antitrypsin, plasminogen, and decreased the expression of FIB and factor XIII in the liver and plasma of HFD-fed mice, leading to inhibition of the intrinsic, extrinsic, and common pathways and activation of fibrinolytic pathway. These events may work in concert to inhibit NAFLD-induced thrombus formation. Abbreviations: SERPING1, serpin family G member 1. Red and purple arrows mean upregulation and downregulation.

Article Snippet: Rabbit antibodies against serpin family G member 1 (SERPING1, 12259-1-AP) and α1-antitrypsin (16382-1-AP) were purchased from Proteintech (Rosemont, IL, USA).

Techniques: Expressing, Inhibition, Activation Assay

Journal: Annals of Translational Medicine

Article Title: Functional hit 1 (FH1)-based rapid and efficient generation of functional hepatocytes from human mesenchymal stem cells: a novel strategy for hepatic differentiation

doi: 10.21037/atm-21-2829

Figure Lengend Snippet: Antibodies used for immunofluorescence and western blotting

Article Snippet: Detailed information of all antibodies is given in . table ft1 table-wrap mode="anchored" t5 caption a7 Marker (species) Application (dilution) Distributor (catalog number) Primary antibodies FoxA2 (rabbit) IF (1:200) Proteintech (22474-1-AP) Sox17 (mouse) IF (1:200) R&D (MAB1924-SP) AFP (rabbit) IF (1:100) Proteintech (14550-1-AP) HNF4α (mouse) IF (1:500) Santa Cruz (sc-374229) ALB (rabbit) IF (1:200) Proteintech (16475-1-AP) A1AT (mouse) IF (1:100) Proteintech (66135-1-Ig) ALB (rabbit) WB (1:5,000) Proteintech (16475-1-AP) AFP (rabbit) WB (1:1,000) Santa Cruz (sc-8399) A1AT (mouse) WB (1:5,000) Proteintech (66135-1-Ig) HNF4α (rabbit) WB (1:1,000) ACTIVE MOTIF (61190) NTCP (mouse) WB (1:1,000) Santa Cruz (Sc-518115) FXR (mouse) WB (1:1,000) Santa Cruz (SC-25309) GAPDH (rabbit) WB (1:5,000) Proteintech (10494-1-AP) Secondary antibodies Goat anti-rabbit IgG-HRP antibody WB (1:10,000) Boster (BA1051) Goat anti- mouse IgG-HRP antibody WB (1:10,000) Boster (BA1054) Goat anti-rabbit IgG secondary antibody IF (1:100) Boster (BA1032) Goat anti-mouse IgG secondary antibody IF (1:100) Boster (BA1101) Open in a separate window caption a8 Antibodies used for immunofluorescence and western blotting Western blot Cells were dissolved in a RIPA lysis buffer, supplemented with protease inhibitor cocktail (Abmole), and after centrifugation at 12,000 g for 10 min at 4 °C, the supernatant was collected.

Techniques: Immunofluorescence, Western Blot

Journal: Annals of Translational Medicine

Article Title: Functional hit 1 (FH1)-based rapid and efficient generation of functional hepatocytes from human mesenchymal stem cells: a novel strategy for hepatic differentiation

doi: 10.21037/atm-21-2829

Figure Lengend Snippet: Primers used for real-time quantitative PCR

Article Snippet: Detailed information of all antibodies is given in . table ft1 table-wrap mode="anchored" t5 caption a7 Marker (species) Application (dilution) Distributor (catalog number) Primary antibodies FoxA2 (rabbit) IF (1:200) Proteintech (22474-1-AP) Sox17 (mouse) IF (1:200) R&D (MAB1924-SP) AFP (rabbit) IF (1:100) Proteintech (14550-1-AP) HNF4α (mouse) IF (1:500) Santa Cruz (sc-374229) ALB (rabbit) IF (1:200) Proteintech (16475-1-AP) A1AT (mouse) IF (1:100) Proteintech (66135-1-Ig) ALB (rabbit) WB (1:5,000) Proteintech (16475-1-AP) AFP (rabbit) WB (1:1,000) Santa Cruz (sc-8399) A1AT (mouse) WB (1:5,000) Proteintech (66135-1-Ig) HNF4α (rabbit) WB (1:1,000) ACTIVE MOTIF (61190) NTCP (mouse) WB (1:1,000) Santa Cruz (Sc-518115) FXR (mouse) WB (1:1,000) Santa Cruz (SC-25309) GAPDH (rabbit) WB (1:5,000) Proteintech (10494-1-AP) Secondary antibodies Goat anti-rabbit IgG-HRP antibody WB (1:10,000) Boster (BA1051) Goat anti- mouse IgG-HRP antibody WB (1:10,000) Boster (BA1054) Goat anti-rabbit IgG secondary antibody IF (1:100) Boster (BA1032) Goat anti-mouse IgG secondary antibody IF (1:100) Boster (BA1101) Open in a separate window caption a8 Antibodies used for immunofluorescence and western blotting

Techniques: Sequencing

Journal: Annals of Translational Medicine

Article Title: Functional hit 1 (FH1)-based rapid and efficient generation of functional hepatocytes from human mesenchymal stem cells: a novel strategy for hepatic differentiation

doi: 10.21037/atm-21-2829

Figure Lengend Snippet: Antibodies used for immunofluorescence and western blotting

Article Snippet: Detailed information of all antibodies is given in . table ft1 table-wrap mode="anchored" t5 caption a7 Marker (species) Application (dilution) Distributor (catalog number) Primary antibodies FoxA2 (rabbit) IF (1:200) Proteintech (22474-1-AP) Sox17 (mouse) IF (1:200) R&D (MAB1924-SP) AFP (rabbit) IF (1:100) Proteintech (14550-1-AP) HNF4α (mouse) IF (1:500) Santa Cruz (sc-374229) ALB (rabbit) IF (1:200) Proteintech (16475-1-AP) A1AT (mouse) IF (1:100) Proteintech (66135-1-Ig) ALB (rabbit) WB (1:5,000) Proteintech (16475-1-AP) AFP (rabbit) WB (1:1,000) Santa Cruz (sc-8399) A1AT (mouse) WB (1:5,000) Proteintech (66135-1-Ig) HNF4α (rabbit) WB (1:1,000) ACTIVE MOTIF (61190) NTCP (mouse) WB (1:1,000) Santa Cruz (Sc-518115) FXR (mouse) WB (1:1,000) Santa Cruz (SC-25309) GAPDH (rabbit) WB (1:5,000) Proteintech (10494-1-AP) Secondary antibodies Goat anti-rabbit IgG-HRP antibody WB (1:10,000) Boster (BA1051) Goat anti- mouse IgG-HRP antibody WB (1:10,000) Boster (BA1054) Goat anti-rabbit IgG secondary antibody IF (1:100) Boster (BA1032) Goat anti-mouse IgG secondary antibody IF (1:100) Boster (BA1101) Open in a separate window caption a8 Antibodies used for immunofluorescence and western blotting

Techniques: Immunofluorescence, Western Blot

Journal: Annals of Translational Medicine

Article Title: Functional hit 1 (FH1)-based rapid and efficient generation of functional hepatocytes from human mesenchymal stem cells: a novel strategy for hepatic differentiation

doi: 10.21037/atm-21-2829

Figure Lengend Snippet: Small molecules efficiently induce DE to differentiate to hepatic progenitors. (A) Morphological changes during hepatic progenitor differentiation. Scale bar =100 µM. (B) qRT-PCR analysis of the mRNA level of hepatic progenitor specific markers expressed in small molecule or growth-factor groups. (C) Immunofluorescent of AFP and HNF4α protein level expression at day 5 (small molecule-induced hepatic progenitor) and day 10 (growth-factor hepatic progenitor). Scale bar =100 µM. (D) Percentage of AFP+ HNF4α+ in immunofluorescence of hepatic progenitors. Undifferentiated human MSCs used as control. All data presented as the mean of at least three independent experiments. *P value <0.05. qRT-PCR, quantitative real-time PCR; SM, small molecule; GF, growth factor; MSC, mesenchymal stem cell; DE, definitive endoderm.

Article Snippet: Detailed information of all antibodies is given in . table ft1 table-wrap mode="anchored" t5 caption a7 Marker (species) Application (dilution) Distributor (catalog number) Primary antibodies FoxA2 (rabbit) IF (1:200) Proteintech (22474-1-AP) Sox17 (mouse) IF (1:200) R&D (MAB1924-SP) AFP (rabbit) IF (1:100) Proteintech (14550-1-AP) HNF4α (mouse) IF (1:500) Santa Cruz (sc-374229) ALB (rabbit) IF (1:200) Proteintech (16475-1-AP) A1AT (mouse) IF (1:100) Proteintech (66135-1-Ig) ALB (rabbit) WB (1:5,000) Proteintech (16475-1-AP) AFP (rabbit) WB (1:1,000) Santa Cruz (sc-8399) A1AT (mouse) WB (1:5,000) Proteintech (66135-1-Ig) HNF4α (rabbit) WB (1:1,000) ACTIVE MOTIF (61190) NTCP (mouse) WB (1:1,000) Santa Cruz (Sc-518115) FXR (mouse) WB (1:1,000) Santa Cruz (SC-25309) GAPDH (rabbit) WB (1:5,000) Proteintech (10494-1-AP) Secondary antibodies Goat anti-rabbit IgG-HRP antibody WB (1:10,000) Boster (BA1051) Goat anti- mouse IgG-HRP antibody WB (1:10,000) Boster (BA1054) Goat anti-rabbit IgG secondary antibody IF (1:100) Boster (BA1032) Goat anti-mouse IgG secondary antibody IF (1:100) Boster (BA1101) Open in a separate window caption a8 Antibodies used for immunofluorescence and western blotting

Techniques: Quantitative RT-PCR, Expressing, Immunofluorescence, Control, Real-time Polymerase Chain Reaction

Journal: Annals of Translational Medicine

Article Title: Functional hit 1 (FH1)-based rapid and efficient generation of functional hepatocytes from human mesenchymal stem cells: a novel strategy for hepatic differentiation

doi: 10.21037/atm-21-2829

Figure Lengend Snippet: Characterization of small molecule- and growth factor-iHeps. (A) Glycogen storage analysis via PAS staining in small molecule- and growth factor-iHeps. Undifferentiated MSCs used as the control. (B) ICG intake, (C) LDL uptake, scale bar =100 µM. (D) Urea production, (E) Western blots for ALB, A1AT, HNF4α, AFP, NTXP, and FXR expression. GAPDH used as a control. (F) mRNA levels of CYP genes were determined by qRT-PCR in human hUC-MSC-iHeps. All data are presented as the mean of at least three independent experiments. *P value <0.05; **P value <0.01. qRT-PCR, Quantitative real-time PCR; MSC, mesenchymal stem cell; GF, growth factor; SM, small molecule; HGF, hepatocyte growth factor; iHeps, induced hepatocytes; FH1, functional hit 1.

Article Snippet: Detailed information of all antibodies is given in . table ft1 table-wrap mode="anchored" t5 caption a7 Marker (species) Application (dilution) Distributor (catalog number) Primary antibodies FoxA2 (rabbit) IF (1:200) Proteintech (22474-1-AP) Sox17 (mouse) IF (1:200) R&D (MAB1924-SP) AFP (rabbit) IF (1:100) Proteintech (14550-1-AP) HNF4α (mouse) IF (1:500) Santa Cruz (sc-374229) ALB (rabbit) IF (1:200) Proteintech (16475-1-AP) A1AT (mouse) IF (1:100) Proteintech (66135-1-Ig) ALB (rabbit) WB (1:5,000) Proteintech (16475-1-AP) AFP (rabbit) WB (1:1,000) Santa Cruz (sc-8399) A1AT (mouse) WB (1:5,000) Proteintech (66135-1-Ig) HNF4α (rabbit) WB (1:1,000) ACTIVE MOTIF (61190) NTCP (mouse) WB (1:1,000) Santa Cruz (Sc-518115) FXR (mouse) WB (1:1,000) Santa Cruz (SC-25309) GAPDH (rabbit) WB (1:5,000) Proteintech (10494-1-AP) Secondary antibodies Goat anti-rabbit IgG-HRP antibody WB (1:10,000) Boster (BA1051) Goat anti- mouse IgG-HRP antibody WB (1:10,000) Boster (BA1054) Goat anti-rabbit IgG secondary antibody IF (1:100) Boster (BA1032) Goat anti-mouse IgG secondary antibody IF (1:100) Boster (BA1101) Open in a separate window caption a8 Antibodies used for immunofluorescence and western blotting

Techniques: Staining, Control, Western Blot, Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction, Functional Assay

Journal: Annals of Translational Medicine

Article Title: Functional hit 1 (FH1)-based rapid and efficient generation of functional hepatocytes from human mesenchymal stem cells: a novel strategy for hepatic differentiation

doi: 10.21037/atm-21-2829

Figure Lengend Snippet: Use of FH1-based differentiation protocol with human iPSC-derived MSCs. (A) Representative cell morphology of hepatocyte-like cells induced from human iMSCs. Scale bar =100 µM. (B) Immunofluorescence of ALB and A1AT protein expression levels at day 10 in human iMSCs-derived hepatocytes. Scale bar =100 µM. (C) Percentage of ALB + A1AT + in immunofluorescence of hepatocyte cells from iMSC (D) qRT-PCR of hepatocyte-specific markers expressed in iMSCs-derived hepatocytes. Undifferentiated human MSCs used as control. (E) ICG intake. (F) LDL uptake. (G) Glycogen storage analysis via PAS staining in iMSC-derived hepatocytes. Scale bar =100 µM. (H) Western blots for ALB, A1AT, HNF4α, NTXP, and FXR expression. GAPDH used as a control. All data are presented as the mean of at least three independent experiments. qRT-PCR, Quantitative real-time PCR; MSC, mesenchymal stem cell; iMSC, induced pluripotent MSCs; iHeps, induced hepatocytes; FH1, functional hit 1.

Article Snippet: Detailed information of all antibodies is given in . table ft1 table-wrap mode="anchored" t5 caption a7 Marker (species) Application (dilution) Distributor (catalog number) Primary antibodies FoxA2 (rabbit) IF (1:200) Proteintech (22474-1-AP) Sox17 (mouse) IF (1:200) R&D (MAB1924-SP) AFP (rabbit) IF (1:100) Proteintech (14550-1-AP) HNF4α (mouse) IF (1:500) Santa Cruz (sc-374229) ALB (rabbit) IF (1:200) Proteintech (16475-1-AP) A1AT (mouse) IF (1:100) Proteintech (66135-1-Ig) ALB (rabbit) WB (1:5,000) Proteintech (16475-1-AP) AFP (rabbit) WB (1:1,000) Santa Cruz (sc-8399) A1AT (mouse) WB (1:5,000) Proteintech (66135-1-Ig) HNF4α (rabbit) WB (1:1,000) ACTIVE MOTIF (61190) NTCP (mouse) WB (1:1,000) Santa Cruz (Sc-518115) FXR (mouse) WB (1:1,000) Santa Cruz (SC-25309) GAPDH (rabbit) WB (1:5,000) Proteintech (10494-1-AP) Secondary antibodies Goat anti-rabbit IgG-HRP antibody WB (1:10,000) Boster (BA1051) Goat anti- mouse IgG-HRP antibody WB (1:10,000) Boster (BA1054) Goat anti-rabbit IgG secondary antibody IF (1:100) Boster (BA1032) Goat anti-mouse IgG secondary antibody IF (1:100) Boster (BA1101) Open in a separate window caption a8 Antibodies used for immunofluorescence and western blotting

Techniques: Derivative Assay, Immunofluorescence, Expressing, Quantitative RT-PCR, Control, Staining, Western Blot, Real-time Polymerase Chain Reaction, Functional Assay