st atcc 6303  (ATCC)


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    ATCC st atcc 6303
    St Atcc 6303, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    st atcc 6303 - by Bioz Stars, 2023-06
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    st atcc 6303  (ATCC)


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    ATCC st atcc 6303
    St Atcc 6303, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/st atcc 6303/product/ATCC
    Average 98 stars, based on 1 article reviews
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    st atcc 6303 - by Bioz Stars, 2023-06
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    s pneumoniae  (ATCC)


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    ATCC s pneumoniae
    (A) Experimental layout of primary influenza (day 0 instillation with 103 PFU influenza A/PR/8/34 H1N1 (PR8) followed by day 14 instillation with PBS), primary S. <t>pneumoniae</t> (day 0 instillation with PBS followed by day 14 instillation with 103 CFU of ATCC 6303), and secondary S. pneumoniae (day 0 instillation with PR8 103 PFU followed by day 14 instillation with 103 CFU of ATCC 6303) infection in young (2 months) and aged (19 months) adult male and female BALB/c mice. (B) Clinical scores (0hr: P=0.0005, 24hr: P=0.0013, and 48hr: P=0.0073), (C) weight changed (P<0.0001), and (D) survival (P=0.0004) in young and aged mice in response to secondary S. pneumoniae infection. (E) Bacterial titer in lung was assessed in lung homogenates collected at 24 hours post infection (primary S. pneumoniae (PBS + S. pne): P=0.0028, secondary S. pneumoniae (INF + S. pne): P=0.0001, young primary vs. secondary S. pneumoniae: P=0.0461, and aged primary vs. secondary S. pneumoniae: P=0.0006). Similar results were obtained from at least two or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.
    S Pneumoniae, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 98 stars, based on 1 article reviews
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    s pneumoniae - by Bioz Stars, 2023-06
    98/100 stars

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    1) Product Images from "Decreased NLRP3 Inflammasome Expression in Aged Lung may contribute to Increased Susceptibility to Secondary Streptococcus pneumoniae Infection"

    Article Title: Decreased NLRP3 Inflammasome Expression in Aged Lung may contribute to Increased Susceptibility to Secondary Streptococcus pneumoniae Infection

    Journal: Experimental gerontology

    doi: 10.1016/j.exger.2017.11.010

    (A) Experimental layout of primary influenza (day 0 instillation with 103 PFU influenza A/PR/8/34 H1N1 (PR8) followed by day 14 instillation with PBS), primary S. pneumoniae (day 0 instillation with PBS followed by day 14 instillation with 103 CFU of ATCC 6303), and secondary S. pneumoniae (day 0 instillation with PR8 103 PFU followed by day 14 instillation with 103 CFU of ATCC 6303) infection in young (2 months) and aged (19 months) adult male and female BALB/c mice. (B) Clinical scores (0hr: P=0.0005, 24hr: P=0.0013, and 48hr: P=0.0073), (C) weight changed (P<0.0001), and (D) survival (P=0.0004) in young and aged mice in response to secondary S. pneumoniae infection. (E) Bacterial titer in lung was assessed in lung homogenates collected at 24 hours post infection (primary S. pneumoniae (PBS + S. pne): P=0.0028, secondary S. pneumoniae (INF + S. pne): P=0.0001, young primary vs. secondary S. pneumoniae: P=0.0461, and aged primary vs. secondary S. pneumoniae: P=0.0006). Similar results were obtained from at least two or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.
    Figure Legend Snippet: (A) Experimental layout of primary influenza (day 0 instillation with 103 PFU influenza A/PR/8/34 H1N1 (PR8) followed by day 14 instillation with PBS), primary S. pneumoniae (day 0 instillation with PBS followed by day 14 instillation with 103 CFU of ATCC 6303), and secondary S. pneumoniae (day 0 instillation with PR8 103 PFU followed by day 14 instillation with 103 CFU of ATCC 6303) infection in young (2 months) and aged (19 months) adult male and female BALB/c mice. (B) Clinical scores (0hr: P=0.0005, 24hr: P=0.0013, and 48hr: P=0.0073), (C) weight changed (P<0.0001), and (D) survival (P=0.0004) in young and aged mice in response to secondary S. pneumoniae infection. (E) Bacterial titer in lung was assessed in lung homogenates collected at 24 hours post infection (primary S. pneumoniae (PBS + S. pne): P=0.0028, secondary S. pneumoniae (INF + S. pne): P=0.0001, young primary vs. secondary S. pneumoniae: P=0.0461, and aged primary vs. secondary S. pneumoniae: P=0.0006). Similar results were obtained from at least two or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.

    Techniques Used: Infection

    (A–C) Young (2 months) and aged (19 months) male and female BALB/c mice received influenza (day 0: PR8, 103 PFU-INF) prior to secondary instillation with S. pneumoniae (day 14, 103 CFU, ATCC 6303- S. pne). Lung tissue was collected at 24 hours post-secondary S. pneumoniae infection. Young and aged lung from influenza-infected mice (day 14 post infection) at 24 hours post PBS instillation were used as calibrator samples. mRNA expression values in young and aged lung in response to secondary S. pneumoniae infection are relative to age-matched calibrator samples. (A) Changes in lung mRNA expression of TLR1 (P=0.0011), TLR2, TLR4, TLR6 (P=0.0223), and TLR9 (P=0.0041) in young and aged lung was assessed by real time PCR. (B) Changes in mRNA expression of NOD1 (P=0.0201), NOD2 (P<0.0001), RIP2 (P=0.0455), and NF-κB at 24 hours post infection were assessed by real time PCR. (C) Inflammatory cytokine expression was investigated in young and aged lung and changes in pro-IL1β (P=0.0169), pro-IL18, IL6, and TNF (P=0.0089) were assessed by real time PCR. (D) TNFα production in lung homogenates was assessed at 24 hours secondary instillation with PBS (day 0 instillation with influenza followed by day 14 instillation with PBS: INF + PBS) or S. pneumoniae (day 0 instillation with influenza followed by day 14 instillation with S. pneumoniae: INF + S. pne) (P=0.0284). Similar results were obtained from at least three or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.
    Figure Legend Snippet: (A–C) Young (2 months) and aged (19 months) male and female BALB/c mice received influenza (day 0: PR8, 103 PFU-INF) prior to secondary instillation with S. pneumoniae (day 14, 103 CFU, ATCC 6303- S. pne). Lung tissue was collected at 24 hours post-secondary S. pneumoniae infection. Young and aged lung from influenza-infected mice (day 14 post infection) at 24 hours post PBS instillation were used as calibrator samples. mRNA expression values in young and aged lung in response to secondary S. pneumoniae infection are relative to age-matched calibrator samples. (A) Changes in lung mRNA expression of TLR1 (P=0.0011), TLR2, TLR4, TLR6 (P=0.0223), and TLR9 (P=0.0041) in young and aged lung was assessed by real time PCR. (B) Changes in mRNA expression of NOD1 (P=0.0201), NOD2 (P<0.0001), RIP2 (P=0.0455), and NF-κB at 24 hours post infection were assessed by real time PCR. (C) Inflammatory cytokine expression was investigated in young and aged lung and changes in pro-IL1β (P=0.0169), pro-IL18, IL6, and TNF (P=0.0089) were assessed by real time PCR. (D) TNFα production in lung homogenates was assessed at 24 hours secondary instillation with PBS (day 0 instillation with influenza followed by day 14 instillation with PBS: INF + PBS) or S. pneumoniae (day 0 instillation with influenza followed by day 14 instillation with S. pneumoniae: INF + S. pne) (P=0.0284). Similar results were obtained from at least three or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.

    Techniques Used: Infection, Expressing, Real-time Polymerase Chain Reaction

    (A–C) A–C) Young (2 months) and aged (19 months) male and female BALB/c mice received influenza (day 0: PR8, 103 PFU-INF) prior to secondary instillation with S. pneumoniae (day 14, 103 CFU, ATCC 6303- S. pne). Serum and lung tissue was collected at 24 hours post-secondary S. pneumoniae infection. Young and aged lung from influenza-infected mice (day 14 post infection) at 24 hours post PBS instillation were used as calibrator samples. mRNA expression values in young and aged lung in response to secondary S. pneumoniae infection are relative to age-matched calibrator samples. (A) Changes in lung mRNA expression of pro-caspase 1, NLRP3 (P=0.001), and ASC in young and aged lung was assessed by real time PCR. IL1β production in (B) serum and (C) lung homogenates (P=0.0147) was assessed by ELISA. Similar results were obtained from at least three or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.
    Figure Legend Snippet: (A–C) A–C) Young (2 months) and aged (19 months) male and female BALB/c mice received influenza (day 0: PR8, 103 PFU-INF) prior to secondary instillation with S. pneumoniae (day 14, 103 CFU, ATCC 6303- S. pne). Serum and lung tissue was collected at 24 hours post-secondary S. pneumoniae infection. Young and aged lung from influenza-infected mice (day 14 post infection) at 24 hours post PBS instillation were used as calibrator samples. mRNA expression values in young and aged lung in response to secondary S. pneumoniae infection are relative to age-matched calibrator samples. (A) Changes in lung mRNA expression of pro-caspase 1, NLRP3 (P=0.001), and ASC in young and aged lung was assessed by real time PCR. IL1β production in (B) serum and (C) lung homogenates (P=0.0147) was assessed by ELISA. Similar results were obtained from at least three or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.

    Techniques Used: Infection, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    s pneumoniae  (ATCC)


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    ATCC s pneumoniae
    (A) Experimental layout of primary influenza (day 0 instillation with 103 PFU influenza A/PR/8/34 H1N1 (PR8) followed by day 14 instillation with PBS), primary S. <t>pneumoniae</t> (day 0 instillation with PBS followed by day 14 instillation with 103 CFU of ATCC 6303), and secondary S. pneumoniae (day 0 instillation with PR8 103 PFU followed by day 14 instillation with 103 CFU of ATCC 6303) infection in young (2 months) and aged (19 months) adult male and female BALB/c mice. (B) Clinical scores (0hr: P=0.0005, 24hr: P=0.0013, and 48hr: P=0.0073), (C) weight changed (P<0.0001), and (D) survival (P=0.0004) in young and aged mice in response to secondary S. pneumoniae infection. (E) Bacterial titer in lung was assessed in lung homogenates collected at 24 hours post infection (primary S. pneumoniae (PBS + S. pne): P=0.0028, secondary S. pneumoniae (INF + S. pne): P=0.0001, young primary vs. secondary S. pneumoniae: P=0.0461, and aged primary vs. secondary S. pneumoniae: P=0.0006). Similar results were obtained from at least two or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.
    S Pneumoniae, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s pneumoniae/product/ATCC
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s pneumoniae - by Bioz Stars, 2023-06
    98/100 stars

    Images

    1) Product Images from "Decreased NLRP3 Inflammasome Expression in Aged Lung may contribute to Increased Susceptibility to Secondary Streptococcus pneumoniae Infection"

    Article Title: Decreased NLRP3 Inflammasome Expression in Aged Lung may contribute to Increased Susceptibility to Secondary Streptococcus pneumoniae Infection

    Journal: Experimental gerontology

    doi: 10.1016/j.exger.2017.11.010

    (A) Experimental layout of primary influenza (day 0 instillation with 103 PFU influenza A/PR/8/34 H1N1 (PR8) followed by day 14 instillation with PBS), primary S. pneumoniae (day 0 instillation with PBS followed by day 14 instillation with 103 CFU of ATCC 6303), and secondary S. pneumoniae (day 0 instillation with PR8 103 PFU followed by day 14 instillation with 103 CFU of ATCC 6303) infection in young (2 months) and aged (19 months) adult male and female BALB/c mice. (B) Clinical scores (0hr: P=0.0005, 24hr: P=0.0013, and 48hr: P=0.0073), (C) weight changed (P<0.0001), and (D) survival (P=0.0004) in young and aged mice in response to secondary S. pneumoniae infection. (E) Bacterial titer in lung was assessed in lung homogenates collected at 24 hours post infection (primary S. pneumoniae (PBS + S. pne): P=0.0028, secondary S. pneumoniae (INF + S. pne): P=0.0001, young primary vs. secondary S. pneumoniae: P=0.0461, and aged primary vs. secondary S. pneumoniae: P=0.0006). Similar results were obtained from at least two or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.
    Figure Legend Snippet: (A) Experimental layout of primary influenza (day 0 instillation with 103 PFU influenza A/PR/8/34 H1N1 (PR8) followed by day 14 instillation with PBS), primary S. pneumoniae (day 0 instillation with PBS followed by day 14 instillation with 103 CFU of ATCC 6303), and secondary S. pneumoniae (day 0 instillation with PR8 103 PFU followed by day 14 instillation with 103 CFU of ATCC 6303) infection in young (2 months) and aged (19 months) adult male and female BALB/c mice. (B) Clinical scores (0hr: P=0.0005, 24hr: P=0.0013, and 48hr: P=0.0073), (C) weight changed (P<0.0001), and (D) survival (P=0.0004) in young and aged mice in response to secondary S. pneumoniae infection. (E) Bacterial titer in lung was assessed in lung homogenates collected at 24 hours post infection (primary S. pneumoniae (PBS + S. pne): P=0.0028, secondary S. pneumoniae (INF + S. pne): P=0.0001, young primary vs. secondary S. pneumoniae: P=0.0461, and aged primary vs. secondary S. pneumoniae: P=0.0006). Similar results were obtained from at least two or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.

    Techniques Used: Infection

    (A–C) Young (2 months) and aged (19 months) male and female BALB/c mice received influenza (day 0: PR8, 103 PFU-INF) prior to secondary instillation with S. pneumoniae (day 14, 103 CFU, ATCC 6303- S. pne). Lung tissue was collected at 24 hours post-secondary S. pneumoniae infection. Young and aged lung from influenza-infected mice (day 14 post infection) at 24 hours post PBS instillation were used as calibrator samples. mRNA expression values in young and aged lung in response to secondary S. pneumoniae infection are relative to age-matched calibrator samples. (A) Changes in lung mRNA expression of TLR1 (P=0.0011), TLR2, TLR4, TLR6 (P=0.0223), and TLR9 (P=0.0041) in young and aged lung was assessed by real time PCR. (B) Changes in mRNA expression of NOD1 (P=0.0201), NOD2 (P<0.0001), RIP2 (P=0.0455), and NF-κB at 24 hours post infection were assessed by real time PCR. (C) Inflammatory cytokine expression was investigated in young and aged lung and changes in pro-IL1β (P=0.0169), pro-IL18, IL6, and TNF (P=0.0089) were assessed by real time PCR. (D) TNFα production in lung homogenates was assessed at 24 hours secondary instillation with PBS (day 0 instillation with influenza followed by day 14 instillation with PBS: INF + PBS) or S. pneumoniae (day 0 instillation with influenza followed by day 14 instillation with S. pneumoniae: INF + S. pne) (P=0.0284). Similar results were obtained from at least three or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.
    Figure Legend Snippet: (A–C) Young (2 months) and aged (19 months) male and female BALB/c mice received influenza (day 0: PR8, 103 PFU-INF) prior to secondary instillation with S. pneumoniae (day 14, 103 CFU, ATCC 6303- S. pne). Lung tissue was collected at 24 hours post-secondary S. pneumoniae infection. Young and aged lung from influenza-infected mice (day 14 post infection) at 24 hours post PBS instillation were used as calibrator samples. mRNA expression values in young and aged lung in response to secondary S. pneumoniae infection are relative to age-matched calibrator samples. (A) Changes in lung mRNA expression of TLR1 (P=0.0011), TLR2, TLR4, TLR6 (P=0.0223), and TLR9 (P=0.0041) in young and aged lung was assessed by real time PCR. (B) Changes in mRNA expression of NOD1 (P=0.0201), NOD2 (P<0.0001), RIP2 (P=0.0455), and NF-κB at 24 hours post infection were assessed by real time PCR. (C) Inflammatory cytokine expression was investigated in young and aged lung and changes in pro-IL1β (P=0.0169), pro-IL18, IL6, and TNF (P=0.0089) were assessed by real time PCR. (D) TNFα production in lung homogenates was assessed at 24 hours secondary instillation with PBS (day 0 instillation with influenza followed by day 14 instillation with PBS: INF + PBS) or S. pneumoniae (day 0 instillation with influenza followed by day 14 instillation with S. pneumoniae: INF + S. pne) (P=0.0284). Similar results were obtained from at least three or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.

    Techniques Used: Infection, Expressing, Real-time Polymerase Chain Reaction

    (A–C) A–C) Young (2 months) and aged (19 months) male and female BALB/c mice received influenza (day 0: PR8, 103 PFU-INF) prior to secondary instillation with S. pneumoniae (day 14, 103 CFU, ATCC 6303- S. pne). Serum and lung tissue was collected at 24 hours post-secondary S. pneumoniae infection. Young and aged lung from influenza-infected mice (day 14 post infection) at 24 hours post PBS instillation were used as calibrator samples. mRNA expression values in young and aged lung in response to secondary S. pneumoniae infection are relative to age-matched calibrator samples. (A) Changes in lung mRNA expression of pro-caspase 1, NLRP3 (P=0.001), and ASC in young and aged lung was assessed by real time PCR. IL1β production in (B) serum and (C) lung homogenates (P=0.0147) was assessed by ELISA. Similar results were obtained from at least three or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.
    Figure Legend Snippet: (A–C) A–C) Young (2 months) and aged (19 months) male and female BALB/c mice received influenza (day 0: PR8, 103 PFU-INF) prior to secondary instillation with S. pneumoniae (day 14, 103 CFU, ATCC 6303- S. pne). Serum and lung tissue was collected at 24 hours post-secondary S. pneumoniae infection. Young and aged lung from influenza-infected mice (day 14 post infection) at 24 hours post PBS instillation were used as calibrator samples. mRNA expression values in young and aged lung in response to secondary S. pneumoniae infection are relative to age-matched calibrator samples. (A) Changes in lung mRNA expression of pro-caspase 1, NLRP3 (P=0.001), and ASC in young and aged lung was assessed by real time PCR. IL1β production in (B) serum and (C) lung homogenates (P=0.0147) was assessed by ELISA. Similar results were obtained from at least three or more independent experiments with an N=5 per experiment. Data are expressed as the mean ± SEM.

    Techniques Used: Infection, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    escherichia coli atcc 9637  (ATCC)


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    ATCC escherichia coli atcc 9637
    Escherichia Coli Atcc 9637, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli atcc 9637/product/ATCC
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    escherichia coli atcc 9637 - by Bioz Stars, 2023-06
    96/100 stars

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