graphene based chitosan films against gram positive  (ATCC)


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    Structured Review

    ATCC graphene based chitosan films against gram positive
    Chemical pathway illustrating the preparation of four fillers <t>(graphene</t> oxide ( GO ), reduced GO ( rGO ), phosphorylated GO ( PGO ), and trimethylsilylated GO ( SiMe 3 GO ) from <t>graphite.</t>
    Graphene Based Chitosan Films Against Gram Positive, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/graphene based chitosan films against gram positive/product/ATCC
    Average 98 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    graphene based chitosan films against gram positive - by Bioz Stars, 2022-09
    98/100 stars

    Images

    1) Product Images from "Chitosan-Functionalized Graphene Nanocomposite Films: Interfacial Interplay and Biological Activity"

    Article Title: Chitosan-Functionalized Graphene Nanocomposite Films: Interfacial Interplay and Biological Activity

    Journal: Materials

    doi: 10.3390/ma13040998

    Chemical pathway illustrating the preparation of four fillers (graphene oxide ( GO ), reduced GO ( rGO ), phosphorylated GO ( PGO ), and trimethylsilylated GO ( SiMe 3 GO ) from graphite.
    Figure Legend Snippet: Chemical pathway illustrating the preparation of four fillers (graphene oxide ( GO ), reduced GO ( rGO ), phosphorylated GO ( PGO ), and trimethylsilylated GO ( SiMe 3 GO ) from graphite.

    Techniques Used:

    Hemolysis ( a ) and hemoglobin adsorption ( b ) of chitosan-reinforced graphene films.
    Figure Legend Snippet: Hemolysis ( a ) and hemoglobin adsorption ( b ) of chitosan-reinforced graphene films.

    Techniques Used: Adsorption

    Catalase activity ( a ) and hemoglobin oxidation ( b ) of chitosan-reinforced graphene films.
    Figure Legend Snippet: Catalase activity ( a ) and hemoglobin oxidation ( b ) of chitosan-reinforced graphene films.

    Techniques Used: Activity Assay

    Preparation of chitosan-reinforced-functionalized graphene films. From top to bottom: raw precursors, their solutions, and their subsequent evaporation to provide transparent films. Digital photos of the four films as prepared. Right. Illustration of the molecular interplay occurring at the nanocomposite interface.
    Figure Legend Snippet: Preparation of chitosan-reinforced-functionalized graphene films. From top to bottom: raw precursors, their solutions, and their subsequent evaporation to provide transparent films. Digital photos of the four films as prepared. Right. Illustration of the molecular interplay occurring at the nanocomposite interface.

    Techniques Used: Evaporation

    2) Product Images from "Plectranthus amboinicus essential oil and carvacrol bioactive against planktonic and biofilm of oxacillin- and vancomycin-resistant Staphylococcus aureus"

    Article Title: Plectranthus amboinicus essential oil and carvacrol bioactive against planktonic and biofilm of oxacillin- and vancomycin-resistant Staphylococcus aureus

    Journal: BMC Complementary and Alternative Medicine

    doi: 10.1186/s12906-017-1968-9

    Optical density of bacterial biofilm in S. aureus ATCC 6538 and S. aureus isolated from food, treated with OEPA and carvacrol in varying concentrations (0.062, 0.125, 0.25, 0.5, 1, 2 and 4 mg mL −1 ). ANOVA, followed by Student-Newman-Keulstest *** p
    Figure Legend Snippet: Optical density of bacterial biofilm in S. aureus ATCC 6538 and S. aureus isolated from food, treated with OEPA and carvacrol in varying concentrations (0.062, 0.125, 0.25, 0.5, 1, 2 and 4 mg mL −1 ). ANOVA, followed by Student-Newman-Keulstest *** p

    Techniques Used: Isolation

    3) Product Images from "Biofilm Growth on Simulated Fracture Fixation Plates Using a Customized CDC Biofilm Reactor for a Sheep Model of Biofilm-Related Infection"

    Article Title: Biofilm Growth on Simulated Fracture Fixation Plates Using a Customized CDC Biofilm Reactor for a Sheep Model of Biofilm-Related Infection

    Journal: Microorganisms

    doi: 10.3390/microorganisms10040759

    Representative SEM images of polymicrobial biofilms of S . aureus ATCC 6538 and P . aeruginosa ATCC 27853. ( A ) Stitched image of multiple high-resolution SEM images of polymicrobial biofilms. The biofilm plumes were noticeably more distinct and larger than those of the monomicrobial biofilms. ( B ) Biofilm communities had three-dimensional connected cotton ball-like morphologies that did not resemble their monomicrobial biofilm morphologies (compare Figure 3 D–F). ( C , D ) Higher resolution images of the polymicrobial biofilms indicated that both cell types were present and appeared to integrate seamlessly with EPS material present.
    Figure Legend Snippet: Representative SEM images of polymicrobial biofilms of S . aureus ATCC 6538 and P . aeruginosa ATCC 27853. ( A ) Stitched image of multiple high-resolution SEM images of polymicrobial biofilms. The biofilm plumes were noticeably more distinct and larger than those of the monomicrobial biofilms. ( B ) Biofilm communities had three-dimensional connected cotton ball-like morphologies that did not resemble their monomicrobial biofilm morphologies (compare Figure 3 D–F). ( C , D ) Higher resolution images of the polymicrobial biofilms indicated that both cell types were present and appeared to integrate seamlessly with EPS material present.

    Techniques Used:

    Representative SEM images of monomicrobial biofilm growth. ( A ) Stitched image collated from multiple high resolution micro graphs showing the growth pattern of MRSA biofilms on a simulated fracture fixation plate. Biofilm growth was more prominent along the border and screw hole regions. The distinct line that runs along the top of the plate indicates the region where the plate was in the slot of the reactor holding arm. ( B ) Higher resolution image of MRSA biofilms indicate that the biofilm colonies had a flat, plateau-like top. ( C ) Representative image of MRSA biofilms showing the presence of EPS materials. ( D ) Biofilm morphology of S. aureus ATCC 6538. Biofilm communities had significant three-dimensional structures that tapered to a point, in contrast to MRSA biofilms which had a plateau-like appearance. ( E ) Stitched image of P aeruginosa ATCC 27853 biofilms on the surface of a simulated fracture fixation plate. Image was collated from multiple high-resolution images. Similar to what was observed for each isolate, biofilms showed relatively uniform coverage across the surface of the simulated fracture fixation plate. ( F ) Higher resolution image of P aeruginosa ATCC 27853 biofilms. This isolate produced sheet-like structures of biofilms as opposed to the distinct plumes of S . aureus .
    Figure Legend Snippet: Representative SEM images of monomicrobial biofilm growth. ( A ) Stitched image collated from multiple high resolution micro graphs showing the growth pattern of MRSA biofilms on a simulated fracture fixation plate. Biofilm growth was more prominent along the border and screw hole regions. The distinct line that runs along the top of the plate indicates the region where the plate was in the slot of the reactor holding arm. ( B ) Higher resolution image of MRSA biofilms indicate that the biofilm colonies had a flat, plateau-like top. ( C ) Representative image of MRSA biofilms showing the presence of EPS materials. ( D ) Biofilm morphology of S. aureus ATCC 6538. Biofilm communities had significant three-dimensional structures that tapered to a point, in contrast to MRSA biofilms which had a plateau-like appearance. ( E ) Stitched image of P aeruginosa ATCC 27853 biofilms on the surface of a simulated fracture fixation plate. Image was collated from multiple high-resolution images. Similar to what was observed for each isolate, biofilms showed relatively uniform coverage across the surface of the simulated fracture fixation plate. ( F ) Higher resolution image of P aeruginosa ATCC 27853 biofilms. This isolate produced sheet-like structures of biofilms as opposed to the distinct plumes of S . aureus .

    Techniques Used: Produced

    Customized CDC biofilm reactor. ( A ) Model of a simulated fracture fixation plate. ( B ) Model of the customized lid with oval openings through which reactor holding arms can be inserted and hold the fixation plates. ( C ) Model of a holding arm into which fixation plates can be placed. ( D ) Model of the reactor lid and holding arms, each with two fixation plates (total of n = 8 plates/reactor). ( E ) Assembled reactor with relevant tubing consistent for reactor use. ( F ) Reactor with “cozies” surrounding the bottom portion; cozies reduced temperature fluctuations due to temperature swings in the lab.
    Figure Legend Snippet: Customized CDC biofilm reactor. ( A ) Model of a simulated fracture fixation plate. ( B ) Model of the customized lid with oval openings through which reactor holding arms can be inserted and hold the fixation plates. ( C ) Model of a holding arm into which fixation plates can be placed. ( D ) Model of the reactor lid and holding arms, each with two fixation plates (total of n = 8 plates/reactor). ( E ) Assembled reactor with relevant tubing consistent for reactor use. ( F ) Reactor with “cozies” surrounding the bottom portion; cozies reduced temperature fluctuations due to temperature swings in the lab.

    Techniques Used:

    Quantification results for monomicrobial and polymicrobial biofilms. MRSA, S. aureus ATCC 6538, and P. aeruginosa ATCC 27853 had similar bioburden levels as monomicrobial biofilms, each having close to 10 9 CFU/plate. S. aureus ATCC 6538 had roughly 10 2 more CFU/plate than P. aeruginosa ATCC 27853 in the polymicrobial biofilms. S. aureus ATCC 6538 in the polymicrobial biofilms had ~0.8 log 10 more CFU than its monomicrobial counterpart, whereas P. aeruginosa ATCC 27853 had ~0.8 log 10 less CFU than its monomicrobial counterpart.
    Figure Legend Snippet: Quantification results for monomicrobial and polymicrobial biofilms. MRSA, S. aureus ATCC 6538, and P. aeruginosa ATCC 27853 had similar bioburden levels as monomicrobial biofilms, each having close to 10 9 CFU/plate. S. aureus ATCC 6538 had roughly 10 2 more CFU/plate than P. aeruginosa ATCC 27853 in the polymicrobial biofilms. S. aureus ATCC 6538 in the polymicrobial biofilms had ~0.8 log 10 more CFU than its monomicrobial counterpart, whereas P. aeruginosa ATCC 27853 had ~0.8 log 10 less CFU than its monomicrobial counterpart.

    Techniques Used: Bioburden Testing

    4) Product Images from "The Antimicrobial and Antibiofilm In Vitro Activity of Liquid and Vapour Phases of Selected Essential Oils against Staphylococcus aureus"

    Article Title: The Antimicrobial and Antibiofilm In Vitro Activity of Liquid and Vapour Phases of Selected Essential Oils against Staphylococcus aureus

    Journal: Pathogens

    doi: 10.3390/pathogens10091207

    Microphotography of the S. aureus ATCC 6538 reference strain biofilm stained with LIVE/DEAD dye. ( A , B )—biofilm exposed to liquid fractions of thyme oil emulsions in concentration 1.6% ( v/v ) ( A.1 – A.3 ) and 0.8% ( v/v ) ( B.1 – B.3 ); ( C.1 – C.3 )—biofilm treated with 0.1% octenidine and 2% phenoxyethanol solution; ( D.1 – D.3 )—untreated cells. The red/orange colour shows staphylococcal cells altered/damaged in result of exposure to liquid T-EO emulsion, while green-coloured cells are non-altered, viable cells. Fluorescence microscope Etaluma 600 (magnification 20×).
    Figure Legend Snippet: Microphotography of the S. aureus ATCC 6538 reference strain biofilm stained with LIVE/DEAD dye. ( A , B )—biofilm exposed to liquid fractions of thyme oil emulsions in concentration 1.6% ( v/v ) ( A.1 – A.3 ) and 0.8% ( v/v ) ( B.1 – B.3 ); ( C.1 – C.3 )—biofilm treated with 0.1% octenidine and 2% phenoxyethanol solution; ( D.1 – D.3 )—untreated cells. The red/orange colour shows staphylococcal cells altered/damaged in result of exposure to liquid T-EO emulsion, while green-coloured cells are non-altered, viable cells. Fluorescence microscope Etaluma 600 (magnification 20×).

    Techniques Used: Staining, Concentration Assay, Fluorescence, Microscopy

    Impact of vapour phase of R-EO on S. aureus ATCC 6538 biofilm. ( A , B )—volumetric data showing untreated and treated biofilm, respectively. ( 1 )—non-altered fragment of biofilms; ( 2 )—loss of biofilm volume. ( C , D )—staphylococcal biofilm cells treated and untreated with R-EO, respectively. The red/orange colour shows staphylococcal cells altered/damaged in result of exposure to vapour R-EO, while green-coloured cells are non-altered, viable cells. Moreover, the more dark (less green) picture is, the less live cells are captured in this particular field of vision.
    Figure Legend Snippet: Impact of vapour phase of R-EO on S. aureus ATCC 6538 biofilm. ( A , B )—volumetric data showing untreated and treated biofilm, respectively. ( 1 )—non-altered fragment of biofilms; ( 2 )—loss of biofilm volume. ( C , D )—staphylococcal biofilm cells treated and untreated with R-EO, respectively. The red/orange colour shows staphylococcal cells altered/damaged in result of exposure to vapour R-EO, while green-coloured cells are non-altered, viable cells. Moreover, the more dark (less green) picture is, the less live cells are captured in this particular field of vision.

    Techniques Used:

    Viability (%) of S. aureus ATCC 6538 biofilm treated with liquid fractions of T-EO (thyme oil) emulsions assessed with LIVE ( green colour ) and TTC ( red colour ) dyes.
    Figure Legend Snippet: Viability (%) of S. aureus ATCC 6538 biofilm treated with liquid fractions of T-EO (thyme oil) emulsions assessed with LIVE ( green colour ) and TTC ( red colour ) dyes.

    Techniques Used:

    Ability of analysed S. aureus strains to form biofilm and assessed with crystal violet (CV) and tetrazolium chloride (TTC) staining.
    Figure Legend Snippet: Ability of analysed S. aureus strains to form biofilm and assessed with crystal violet (CV) and tetrazolium chloride (TTC) staining.

    Techniques Used: Staining

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  • 98
    ATCC graphene based chitosan films against gram positive
    Chemical pathway illustrating the preparation of four fillers <t>(graphene</t> oxide ( GO ), reduced GO ( rGO ), phosphorylated GO ( PGO ), and trimethylsilylated GO ( SiMe 3 GO ) from <t>graphite.</t>
    Graphene Based Chitosan Films Against Gram Positive, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/graphene based chitosan films against gram positive/product/ATCC
    Average 98 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    graphene based chitosan films against gram positive - by Bioz Stars, 2022-09
    98/100 stars
      Buy from Supplier

    95
    ATCC s aureus atcc 6538 strain
    Microphotography of the S. aureus <t>ATCC</t> 6538 reference strain biofilm stained with LIVE/DEAD dye. ( A , B )—biofilm exposed to liquid fractions of thyme oil emulsions in concentration 1.6% ( v/v ) ( A.1 – A.3 ) and 0.8% ( v/v ) ( B.1 – B.3 ); ( C.1 – C.3 )—biofilm treated with 0.1% octenidine and 2% phenoxyethanol solution; ( D.1 – D.3 )—untreated cells. The red/orange colour shows staphylococcal cells altered/damaged in result of exposure to liquid T-EO emulsion, while green-coloured cells are non-altered, viable cells. Fluorescence microscope Etaluma 600 (magnification 20×).
    S Aureus Atcc 6538 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s aureus atcc 6538 strain/product/ATCC
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    s aureus atcc 6538 strain - by Bioz Stars, 2022-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Chemical pathway illustrating the preparation of four fillers (graphene oxide ( GO ), reduced GO ( rGO ), phosphorylated GO ( PGO ), and trimethylsilylated GO ( SiMe 3 GO ) from graphite.

    Journal: Materials

    Article Title: Chitosan-Functionalized Graphene Nanocomposite Films: Interfacial Interplay and Biological Activity

    doi: 10.3390/ma13040998

    Figure Lengend Snippet: Chemical pathway illustrating the preparation of four fillers (graphene oxide ( GO ), reduced GO ( rGO ), phosphorylated GO ( PGO ), and trimethylsilylated GO ( SiMe 3 GO ) from graphite.

    Article Snippet: In particular, we demonstrated the highest antibacterial activity of graphene-based chitosan films against Gram-positive (S. aureus ATCC 6538) and Gram-negative (E. coli ATCC 25922) strains compared to neat chitosan films.

    Techniques:

    Hemolysis ( a ) and hemoglobin adsorption ( b ) of chitosan-reinforced graphene films.

    Journal: Materials

    Article Title: Chitosan-Functionalized Graphene Nanocomposite Films: Interfacial Interplay and Biological Activity

    doi: 10.3390/ma13040998

    Figure Lengend Snippet: Hemolysis ( a ) and hemoglobin adsorption ( b ) of chitosan-reinforced graphene films.

    Article Snippet: In particular, we demonstrated the highest antibacterial activity of graphene-based chitosan films against Gram-positive (S. aureus ATCC 6538) and Gram-negative (E. coli ATCC 25922) strains compared to neat chitosan films.

    Techniques: Adsorption

    Catalase activity ( a ) and hemoglobin oxidation ( b ) of chitosan-reinforced graphene films.

    Journal: Materials

    Article Title: Chitosan-Functionalized Graphene Nanocomposite Films: Interfacial Interplay and Biological Activity

    doi: 10.3390/ma13040998

    Figure Lengend Snippet: Catalase activity ( a ) and hemoglobin oxidation ( b ) of chitosan-reinforced graphene films.

    Article Snippet: In particular, we demonstrated the highest antibacterial activity of graphene-based chitosan films against Gram-positive (S. aureus ATCC 6538) and Gram-negative (E. coli ATCC 25922) strains compared to neat chitosan films.

    Techniques: Activity Assay

    Preparation of chitosan-reinforced-functionalized graphene films. From top to bottom: raw precursors, their solutions, and their subsequent evaporation to provide transparent films. Digital photos of the four films as prepared. Right. Illustration of the molecular interplay occurring at the nanocomposite interface.

    Journal: Materials

    Article Title: Chitosan-Functionalized Graphene Nanocomposite Films: Interfacial Interplay and Biological Activity

    doi: 10.3390/ma13040998

    Figure Lengend Snippet: Preparation of chitosan-reinforced-functionalized graphene films. From top to bottom: raw precursors, their solutions, and their subsequent evaporation to provide transparent films. Digital photos of the four films as prepared. Right. Illustration of the molecular interplay occurring at the nanocomposite interface.

    Article Snippet: In particular, we demonstrated the highest antibacterial activity of graphene-based chitosan films against Gram-positive (S. aureus ATCC 6538) and Gram-negative (E. coli ATCC 25922) strains compared to neat chitosan films.

    Techniques: Evaporation

    Optical density of bacterial biofilm in S. aureus ATCC 6538 and S. aureus isolated from food, treated with OEPA and carvacrol in varying concentrations (0.062, 0.125, 0.25, 0.5, 1, 2 and 4 mg mL −1 ). ANOVA, followed by Student-Newman-Keulstest *** p

    Journal: BMC Complementary and Alternative Medicine

    Article Title: Plectranthus amboinicus essential oil and carvacrol bioactive against planktonic and biofilm of oxacillin- and vancomycin-resistant Staphylococcus aureus

    doi: 10.1186/s12906-017-1968-9

    Figure Lengend Snippet: Optical density of bacterial biofilm in S. aureus ATCC 6538 and S. aureus isolated from food, treated with OEPA and carvacrol in varying concentrations (0.062, 0.125, 0.25, 0.5, 1, 2 and 4 mg mL −1 ). ANOVA, followed by Student-Newman-Keulstest *** p

    Article Snippet: Tetracycline inhibits biofilm formation (S. aureus ATCC 6538 and OVRSA) in all concentrations tested (0.062, 0.125, 0.25, 0.5, 1, 2 and 4 mg mL−1 ).

    Techniques: Isolation

    Representative SEM images of polymicrobial biofilms of S . aureus ATCC 6538 and P . aeruginosa ATCC 27853. ( A ) Stitched image of multiple high-resolution SEM images of polymicrobial biofilms. The biofilm plumes were noticeably more distinct and larger than those of the monomicrobial biofilms. ( B ) Biofilm communities had three-dimensional connected cotton ball-like morphologies that did not resemble their monomicrobial biofilm morphologies (compare Figure 3 D–F). ( C , D ) Higher resolution images of the polymicrobial biofilms indicated that both cell types were present and appeared to integrate seamlessly with EPS material present.

    Journal: Microorganisms

    Article Title: Biofilm Growth on Simulated Fracture Fixation Plates Using a Customized CDC Biofilm Reactor for a Sheep Model of Biofilm-Related Infection

    doi: 10.3390/microorganisms10040759

    Figure Lengend Snippet: Representative SEM images of polymicrobial biofilms of S . aureus ATCC 6538 and P . aeruginosa ATCC 27853. ( A ) Stitched image of multiple high-resolution SEM images of polymicrobial biofilms. The biofilm plumes were noticeably more distinct and larger than those of the monomicrobial biofilms. ( B ) Biofilm communities had three-dimensional connected cotton ball-like morphologies that did not resemble their monomicrobial biofilm morphologies (compare Figure 3 D–F). ( C , D ) Higher resolution images of the polymicrobial biofilms indicated that both cell types were present and appeared to integrate seamlessly with EPS material present.

    Article Snippet: Quantification data indicated that there were more S. aureus ATCC 6538 cells in the polymicrobial biofilms than P. aeruginosa ATCC 27853.

    Techniques:

    Representative SEM images of monomicrobial biofilm growth. ( A ) Stitched image collated from multiple high resolution micro graphs showing the growth pattern of MRSA biofilms on a simulated fracture fixation plate. Biofilm growth was more prominent along the border and screw hole regions. The distinct line that runs along the top of the plate indicates the region where the plate was in the slot of the reactor holding arm. ( B ) Higher resolution image of MRSA biofilms indicate that the biofilm colonies had a flat, plateau-like top. ( C ) Representative image of MRSA biofilms showing the presence of EPS materials. ( D ) Biofilm morphology of S. aureus ATCC 6538. Biofilm communities had significant three-dimensional structures that tapered to a point, in contrast to MRSA biofilms which had a plateau-like appearance. ( E ) Stitched image of P aeruginosa ATCC 27853 biofilms on the surface of a simulated fracture fixation plate. Image was collated from multiple high-resolution images. Similar to what was observed for each isolate, biofilms showed relatively uniform coverage across the surface of the simulated fracture fixation plate. ( F ) Higher resolution image of P aeruginosa ATCC 27853 biofilms. This isolate produced sheet-like structures of biofilms as opposed to the distinct plumes of S . aureus .

    Journal: Microorganisms

    Article Title: Biofilm Growth on Simulated Fracture Fixation Plates Using a Customized CDC Biofilm Reactor for a Sheep Model of Biofilm-Related Infection

    doi: 10.3390/microorganisms10040759

    Figure Lengend Snippet: Representative SEM images of monomicrobial biofilm growth. ( A ) Stitched image collated from multiple high resolution micro graphs showing the growth pattern of MRSA biofilms on a simulated fracture fixation plate. Biofilm growth was more prominent along the border and screw hole regions. The distinct line that runs along the top of the plate indicates the region where the plate was in the slot of the reactor holding arm. ( B ) Higher resolution image of MRSA biofilms indicate that the biofilm colonies had a flat, plateau-like top. ( C ) Representative image of MRSA biofilms showing the presence of EPS materials. ( D ) Biofilm morphology of S. aureus ATCC 6538. Biofilm communities had significant three-dimensional structures that tapered to a point, in contrast to MRSA biofilms which had a plateau-like appearance. ( E ) Stitched image of P aeruginosa ATCC 27853 biofilms on the surface of a simulated fracture fixation plate. Image was collated from multiple high-resolution images. Similar to what was observed for each isolate, biofilms showed relatively uniform coverage across the surface of the simulated fracture fixation plate. ( F ) Higher resolution image of P aeruginosa ATCC 27853 biofilms. This isolate produced sheet-like structures of biofilms as opposed to the distinct plumes of S . aureus .

    Article Snippet: Quantification data indicated that there were more S. aureus ATCC 6538 cells in the polymicrobial biofilms than P. aeruginosa ATCC 27853.

    Techniques: Produced

    Customized CDC biofilm reactor. ( A ) Model of a simulated fracture fixation plate. ( B ) Model of the customized lid with oval openings through which reactor holding arms can be inserted and hold the fixation plates. ( C ) Model of a holding arm into which fixation plates can be placed. ( D ) Model of the reactor lid and holding arms, each with two fixation plates (total of n = 8 plates/reactor). ( E ) Assembled reactor with relevant tubing consistent for reactor use. ( F ) Reactor with “cozies” surrounding the bottom portion; cozies reduced temperature fluctuations due to temperature swings in the lab.

    Journal: Microorganisms

    Article Title: Biofilm Growth on Simulated Fracture Fixation Plates Using a Customized CDC Biofilm Reactor for a Sheep Model of Biofilm-Related Infection

    doi: 10.3390/microorganisms10040759

    Figure Lengend Snippet: Customized CDC biofilm reactor. ( A ) Model of a simulated fracture fixation plate. ( B ) Model of the customized lid with oval openings through which reactor holding arms can be inserted and hold the fixation plates. ( C ) Model of a holding arm into which fixation plates can be placed. ( D ) Model of the reactor lid and holding arms, each with two fixation plates (total of n = 8 plates/reactor). ( E ) Assembled reactor with relevant tubing consistent for reactor use. ( F ) Reactor with “cozies” surrounding the bottom portion; cozies reduced temperature fluctuations due to temperature swings in the lab.

    Article Snippet: Quantification data indicated that there were more S. aureus ATCC 6538 cells in the polymicrobial biofilms than P. aeruginosa ATCC 27853.

    Techniques:

    Quantification results for monomicrobial and polymicrobial biofilms. MRSA, S. aureus ATCC 6538, and P. aeruginosa ATCC 27853 had similar bioburden levels as monomicrobial biofilms, each having close to 10 9 CFU/plate. S. aureus ATCC 6538 had roughly 10 2 more CFU/plate than P. aeruginosa ATCC 27853 in the polymicrobial biofilms. S. aureus ATCC 6538 in the polymicrobial biofilms had ~0.8 log 10 more CFU than its monomicrobial counterpart, whereas P. aeruginosa ATCC 27853 had ~0.8 log 10 less CFU than its monomicrobial counterpart.

    Journal: Microorganisms

    Article Title: Biofilm Growth on Simulated Fracture Fixation Plates Using a Customized CDC Biofilm Reactor for a Sheep Model of Biofilm-Related Infection

    doi: 10.3390/microorganisms10040759

    Figure Lengend Snippet: Quantification results for monomicrobial and polymicrobial biofilms. MRSA, S. aureus ATCC 6538, and P. aeruginosa ATCC 27853 had similar bioburden levels as monomicrobial biofilms, each having close to 10 9 CFU/plate. S. aureus ATCC 6538 had roughly 10 2 more CFU/plate than P. aeruginosa ATCC 27853 in the polymicrobial biofilms. S. aureus ATCC 6538 in the polymicrobial biofilms had ~0.8 log 10 more CFU than its monomicrobial counterpart, whereas P. aeruginosa ATCC 27853 had ~0.8 log 10 less CFU than its monomicrobial counterpart.

    Article Snippet: Quantification data indicated that there were more S. aureus ATCC 6538 cells in the polymicrobial biofilms than P. aeruginosa ATCC 27853.

    Techniques: Bioburden Testing

    Microphotography of the S. aureus ATCC 6538 reference strain biofilm stained with LIVE/DEAD dye. ( A , B )—biofilm exposed to liquid fractions of thyme oil emulsions in concentration 1.6% ( v/v ) ( A.1 – A.3 ) and 0.8% ( v/v ) ( B.1 – B.3 ); ( C.1 – C.3 )—biofilm treated with 0.1% octenidine and 2% phenoxyethanol solution; ( D.1 – D.3 )—untreated cells. The red/orange colour shows staphylococcal cells altered/damaged in result of exposure to liquid T-EO emulsion, while green-coloured cells are non-altered, viable cells. Fluorescence microscope Etaluma 600 (magnification 20×).

    Journal: Pathogens

    Article Title: The Antimicrobial and Antibiofilm In Vitro Activity of Liquid and Vapour Phases of Selected Essential Oils against Staphylococcus aureus

    doi: 10.3390/pathogens10091207

    Figure Lengend Snippet: Microphotography of the S. aureus ATCC 6538 reference strain biofilm stained with LIVE/DEAD dye. ( A , B )—biofilm exposed to liquid fractions of thyme oil emulsions in concentration 1.6% ( v/v ) ( A.1 – A.3 ) and 0.8% ( v/v ) ( B.1 – B.3 ); ( C.1 – C.3 )—biofilm treated with 0.1% octenidine and 2% phenoxyethanol solution; ( D.1 – D.3 )—untreated cells. The red/orange colour shows staphylococcal cells altered/damaged in result of exposure to liquid T-EO emulsion, while green-coloured cells are non-altered, viable cells. Fluorescence microscope Etaluma 600 (magnification 20×).

    Article Snippet: Firstly, the influence of different Tween 20 concentrations on the growth of planktonic forms of S. aureus ATCC 6538 strain was evaluated.

    Techniques: Staining, Concentration Assay, Fluorescence, Microscopy

    Impact of vapour phase of R-EO on S. aureus ATCC 6538 biofilm. ( A , B )—volumetric data showing untreated and treated biofilm, respectively. ( 1 )—non-altered fragment of biofilms; ( 2 )—loss of biofilm volume. ( C , D )—staphylococcal biofilm cells treated and untreated with R-EO, respectively. The red/orange colour shows staphylococcal cells altered/damaged in result of exposure to vapour R-EO, while green-coloured cells are non-altered, viable cells. Moreover, the more dark (less green) picture is, the less live cells are captured in this particular field of vision.

    Journal: Pathogens

    Article Title: The Antimicrobial and Antibiofilm In Vitro Activity of Liquid and Vapour Phases of Selected Essential Oils against Staphylococcus aureus

    doi: 10.3390/pathogens10091207

    Figure Lengend Snippet: Impact of vapour phase of R-EO on S. aureus ATCC 6538 biofilm. ( A , B )—volumetric data showing untreated and treated biofilm, respectively. ( 1 )—non-altered fragment of biofilms; ( 2 )—loss of biofilm volume. ( C , D )—staphylococcal biofilm cells treated and untreated with R-EO, respectively. The red/orange colour shows staphylococcal cells altered/damaged in result of exposure to vapour R-EO, while green-coloured cells are non-altered, viable cells. Moreover, the more dark (less green) picture is, the less live cells are captured in this particular field of vision.

    Article Snippet: Firstly, the influence of different Tween 20 concentrations on the growth of planktonic forms of S. aureus ATCC 6538 strain was evaluated.

    Techniques:

    Viability (%) of S. aureus ATCC 6538 biofilm treated with liquid fractions of T-EO (thyme oil) emulsions assessed with LIVE ( green colour ) and TTC ( red colour ) dyes.

    Journal: Pathogens

    Article Title: The Antimicrobial and Antibiofilm In Vitro Activity of Liquid and Vapour Phases of Selected Essential Oils against Staphylococcus aureus

    doi: 10.3390/pathogens10091207

    Figure Lengend Snippet: Viability (%) of S. aureus ATCC 6538 biofilm treated with liquid fractions of T-EO (thyme oil) emulsions assessed with LIVE ( green colour ) and TTC ( red colour ) dyes.

    Article Snippet: Firstly, the influence of different Tween 20 concentrations on the growth of planktonic forms of S. aureus ATCC 6538 strain was evaluated.

    Techniques: