Graphene Based Chitosan Films Against Gram Positive, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Chitosan-Functionalized Graphene Nanocomposite Films: Interfacial Interplay and Biological Activity"
Article Title: Chitosan-Functionalized Graphene Nanocomposite Films: Interfacial Interplay and Biological Activity
Figure Legend Snippet: Chemical pathway illustrating the preparation of four fillers (graphene oxide ( GO ), reduced GO ( rGO ), phosphorylated GO ( PGO ), and trimethylsilylated GO ( SiMe 3 GO ) from graphite.
Figure Legend Snippet: Hemolysis ( a ) and hemoglobin adsorption ( b ) of chitosan-reinforced graphene films.
Techniques Used: Adsorption
Figure Legend Snippet: Catalase activity ( a ) and hemoglobin oxidation ( b ) of chitosan-reinforced graphene films.
Techniques Used: Activity Assay
Figure Legend Snippet: Preparation of chitosan-reinforced-functionalized graphene films. From top to bottom: raw precursors, their solutions, and their subsequent evaporation to provide transparent films. Digital photos of the four films as prepared. Right. Illustration of the molecular interplay occurring at the nanocomposite interface.
Techniques Used: Evaporation
2) Product Images from "Plectranthus amboinicus essential oil and carvacrol bioactive against planktonic and biofilm of oxacillin- and vancomycin-resistant Staphylococcus aureus"
Article Title: Plectranthus amboinicus essential oil and carvacrol bioactive against planktonic and biofilm of oxacillin- and vancomycin-resistant Staphylococcus aureus
Journal: BMC Complementary and Alternative Medicine
Figure Legend Snippet: Optical density of bacterial biofilm in S. aureus ATCC 6538 and S. aureus isolated from food, treated with OEPA and carvacrol in varying concentrations (0.062, 0.125, 0.25, 0.5, 1, 2 and 4 mg mL −1 ). ANOVA, followed by Student-Newman-Keulstest *** p
Techniques Used: Isolation
3) Product Images from "Biofilm Growth on Simulated Fracture Fixation Plates Using a Customized CDC Biofilm Reactor for a Sheep Model of Biofilm-Related Infection"
Article Title: Biofilm Growth on Simulated Fracture Fixation Plates Using a Customized CDC Biofilm Reactor for a Sheep Model of Biofilm-Related Infection
Figure Legend Snippet: Representative SEM images of polymicrobial biofilms of S . aureus ATCC 6538 and P . aeruginosa ATCC 27853. ( A ) Stitched image of multiple high-resolution SEM images of polymicrobial biofilms. The biofilm plumes were noticeably more distinct and larger than those of the monomicrobial biofilms. ( B ) Biofilm communities had three-dimensional connected cotton ball-like morphologies that did not resemble their monomicrobial biofilm morphologies (compare Figure 3 D–F). ( C , D ) Higher resolution images of the polymicrobial biofilms indicated that both cell types were present and appeared to integrate seamlessly with EPS material present.
Figure Legend Snippet: Representative SEM images of monomicrobial biofilm growth. ( A ) Stitched image collated from multiple high resolution micro graphs showing the growth pattern of MRSA biofilms on a simulated fracture fixation plate. Biofilm growth was more prominent along the border and screw hole regions. The distinct line that runs along the top of the plate indicates the region where the plate was in the slot of the reactor holding arm. ( B ) Higher resolution image of MRSA biofilms indicate that the biofilm colonies had a flat, plateau-like top. ( C ) Representative image of MRSA biofilms showing the presence of EPS materials. ( D ) Biofilm morphology of S. aureus ATCC 6538. Biofilm communities had significant three-dimensional structures that tapered to a point, in contrast to MRSA biofilms which had a plateau-like appearance. ( E ) Stitched image of P aeruginosa ATCC 27853 biofilms on the surface of a simulated fracture fixation plate. Image was collated from multiple high-resolution images. Similar to what was observed for each isolate, biofilms showed relatively uniform coverage across the surface of the simulated fracture fixation plate. ( F ) Higher resolution image of P aeruginosa ATCC 27853 biofilms. This isolate produced sheet-like structures of biofilms as opposed to the distinct plumes of S . aureus .
Techniques Used: Produced
Figure Legend Snippet: Customized CDC biofilm reactor. ( A ) Model of a simulated fracture fixation plate. ( B ) Model of the customized lid with oval openings through which reactor holding arms can be inserted and hold the fixation plates. ( C ) Model of a holding arm into which fixation plates can be placed. ( D ) Model of the reactor lid and holding arms, each with two fixation plates (total of n = 8 plates/reactor). ( E ) Assembled reactor with relevant tubing consistent for reactor use. ( F ) Reactor with “cozies” surrounding the bottom portion; cozies reduced temperature fluctuations due to temperature swings in the lab.
Figure Legend Snippet: Quantification results for monomicrobial and polymicrobial biofilms. MRSA, S. aureus ATCC 6538, and P. aeruginosa ATCC 27853 had similar bioburden levels as monomicrobial biofilms, each having close to 10 9 CFU/plate. S. aureus ATCC 6538 had roughly 10 2 more CFU/plate than P. aeruginosa ATCC 27853 in the polymicrobial biofilms. S. aureus ATCC 6538 in the polymicrobial biofilms had ~0.8 log 10 more CFU than its monomicrobial counterpart, whereas P. aeruginosa ATCC 27853 had ~0.8 log 10 less CFU than its monomicrobial counterpart.
Techniques Used: Bioburden Testing
4) Product Images from "The Antimicrobial and Antibiofilm In Vitro Activity of Liquid and Vapour Phases of Selected Essential Oils against Staphylococcus aureus"
Article Title: The Antimicrobial and Antibiofilm In Vitro Activity of Liquid and Vapour Phases of Selected Essential Oils against Staphylococcus aureus
Figure Legend Snippet: Microphotography of the S. aureus ATCC 6538 reference strain biofilm stained with LIVE/DEAD dye. ( A , B )—biofilm exposed to liquid fractions of thyme oil emulsions in concentration 1.6% ( v/v ) ( A.1 – A.3 ) and 0.8% ( v/v ) ( B.1 – B.3 ); ( C.1 – C.3 )—biofilm treated with 0.1% octenidine and 2% phenoxyethanol solution; ( D.1 – D.3 )—untreated cells. The red/orange colour shows staphylococcal cells altered/damaged in result of exposure to liquid T-EO emulsion, while green-coloured cells are non-altered, viable cells. Fluorescence microscope Etaluma 600 (magnification 20×).
Techniques Used: Staining, Concentration Assay, Fluorescence, Microscopy
Figure Legend Snippet: Impact of vapour phase of R-EO on S. aureus ATCC 6538 biofilm. ( A , B )—volumetric data showing untreated and treated biofilm, respectively. ( 1 )—non-altered fragment of biofilms; ( 2 )—loss of biofilm volume. ( C , D )—staphylococcal biofilm cells treated and untreated with R-EO, respectively. The red/orange colour shows staphylococcal cells altered/damaged in result of exposure to vapour R-EO, while green-coloured cells are non-altered, viable cells. Moreover, the more dark (less green) picture is, the less live cells are captured in this particular field of vision.
Figure Legend Snippet: Viability (%) of S. aureus ATCC 6538 biofilm treated with liquid fractions of T-EO (thyme oil) emulsions assessed with LIVE ( green colour ) and TTC ( red colour ) dyes.
Figure Legend Snippet: Ability of analysed S. aureus strains to form biofilm and assessed with crystal violet (CV) and tetrazolium chloride (TTC) staining.
Techniques Used: Staining