b mycoides type atcc 6462 strain  (ATCC)


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    Structured Review

    ATCC b mycoides type atcc 6462 strain
    Ribotyping in Bacillus strains. A blot of EcoRI digested total DNA was hybridized by a probe specific for an evolutionarily conserved region of 16S rRNA. Various members of the B. cereus group show different patterns: lane 1, B. <t>mycoides</t> <t>ATCC</t> 6462 T ; lane 2, B. mycoides DX; lane 3, B. pseudomycoides NRRL B-617 T ; lane 4, B. mycoides NRRL NRS-273; lane 5, B. thuringiensis BGSC 4D; lane 6, B. mycoides SIN. Mutant strains all derived from SIN96, with identical patterns: lane 7, B. mycoides SINett; lane 8, CAD; lane 9, CIC; lane 10–13 cotton-like mutants.
    B Mycoides Type Atcc 6462 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Colony shape as a genetic trait in the pattern-forming Bacillus mycoides"

    Article Title: Colony shape as a genetic trait in the pattern-forming Bacillus mycoides

    Journal: BMC Microbiology

    doi: 10.1186/1471-2180-2-33

    Ribotyping in Bacillus strains. A blot of EcoRI digested total DNA was hybridized by a probe specific for an evolutionarily conserved region of 16S rRNA. Various members of the B. cereus group show different patterns: lane 1, B. mycoides ATCC 6462 T ; lane 2, B. mycoides DX; lane 3, B. pseudomycoides NRRL B-617 T ; lane 4, B. mycoides NRRL NRS-273; lane 5, B. thuringiensis BGSC 4D; lane 6, B. mycoides SIN. Mutant strains all derived from SIN96, with identical patterns: lane 7, B. mycoides SINett; lane 8, CAD; lane 9, CIC; lane 10–13 cotton-like mutants.
    Figure Legend Snippet: Ribotyping in Bacillus strains. A blot of EcoRI digested total DNA was hybridized by a probe specific for an evolutionarily conserved region of 16S rRNA. Various members of the B. cereus group show different patterns: lane 1, B. mycoides ATCC 6462 T ; lane 2, B. mycoides DX; lane 3, B. pseudomycoides NRRL B-617 T ; lane 4, B. mycoides NRRL NRS-273; lane 5, B. thuringiensis BGSC 4D; lane 6, B. mycoides SIN. Mutant strains all derived from SIN96, with identical patterns: lane 7, B. mycoides SINett; lane 8, CAD; lane 9, CIC; lane 10–13 cotton-like mutants.

    Techniques Used: Mutagenesis, Derivative Assay

    2) Product Images from "Development of Safirinium dyes for new applications: fluorescent staining of bacteria, human kidney cells, and the horny layer of the epidermis"

    Article Title: Development of Safirinium dyes for new applications: fluorescent staining of bacteria, human kidney cells, and the horny layer of the epidermis

    Journal: Scientific Reports

    doi: 10.1038/s41598-022-19262-w

    Phase contrast and fluorescence microscopy visualization of non-permebilized: ( A ) free floating E. coli and ( B ) aggregates of B. mycoides . Control— E. coli BL21DE3 and B. mycoides incubated without dyes, GFP— E. coli BL21DE3 producing GFP protein, 5l and 6q — E. coli BL21DE3 and B. mycoides stained with the appropriate dye, PC—phase-contrast microscopy and UV—fluorescence microscopy. Scale bars correspond to 20 µm.
    Figure Legend Snippet: Phase contrast and fluorescence microscopy visualization of non-permebilized: ( A ) free floating E. coli and ( B ) aggregates of B. mycoides . Control— E. coli BL21DE3 and B. mycoides incubated without dyes, GFP— E. coli BL21DE3 producing GFP protein, 5l and 6q — E. coli BL21DE3 and B. mycoides stained with the appropriate dye, PC—phase-contrast microscopy and UV—fluorescence microscopy. Scale bars correspond to 20 µm.

    Techniques Used: Fluorescence, Microscopy, Incubation, Staining

    3) Product Images from "Discovering, Characterizing, and Applying Acyl Homoserine Lactone-Quenching Enzymes to Mitigate Microbe-Associated Problems Under Saline Conditions"

    Article Title: Discovering, Characterizing, and Applying Acyl Homoserine Lactone-Quenching Enzymes to Mitigate Microbe-Associated Problems Under Saline Conditions

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2019.00823

    AHL quenching activity comparison of eight potential protein-coding genes expressed in recombinant Escherichia coli cells. The relative AHL quenching efficiency of each sample was calculated based on Eq. (1), as stated in the “Materials and Methods” section. The E. coli TOP10 carrying vector pTricHisA and E. coli BL21 (DE3) carrying pET-20b(+) were used as negative controls. AiiA S1-5 (acyl homoserine lactonase), SDR S1-5 (oxidoreductase), Est S1-5 (esterase), and Hyd S1-5 (hydrolase) are possible AHL quenching ORFs from Altererythrobacter sp. S1-5; Hyd L11 (hydrolase), PvdQ L11 (penicillin acylase family protein), SDR L11 (oxidoreductase), and H PL11 (hypothetical protein) are possible AHL quenching ORFs from Pseudoalteromonas sp. L11. PC denotes a positive control, which is obtained by inserting an AiiA gene from Bacillus mycoides ATCC 6462 into pET-20b(+) and expressing it in E. coli BL21 (DE3). Two independent biological replicates with three technical replicates in each biological replicate were performed for this experiment.
    Figure Legend Snippet: AHL quenching activity comparison of eight potential protein-coding genes expressed in recombinant Escherichia coli cells. The relative AHL quenching efficiency of each sample was calculated based on Eq. (1), as stated in the “Materials and Methods” section. The E. coli TOP10 carrying vector pTricHisA and E. coli BL21 (DE3) carrying pET-20b(+) were used as negative controls. AiiA S1-5 (acyl homoserine lactonase), SDR S1-5 (oxidoreductase), Est S1-5 (esterase), and Hyd S1-5 (hydrolase) are possible AHL quenching ORFs from Altererythrobacter sp. S1-5; Hyd L11 (hydrolase), PvdQ L11 (penicillin acylase family protein), SDR L11 (oxidoreductase), and H PL11 (hypothetical protein) are possible AHL quenching ORFs from Pseudoalteromonas sp. L11. PC denotes a positive control, which is obtained by inserting an AiiA gene from Bacillus mycoides ATCC 6462 into pET-20b(+) and expressing it in E. coli BL21 (DE3). Two independent biological replicates with three technical replicates in each biological replicate were performed for this experiment.

    Techniques Used: Activity Assay, Recombinant, Plasmid Preparation, Positron Emission Tomography, Positive Control, Expressing

    4) Product Images from "Discovering, Characterizing, and Applying Acyl Homoserine Lactone-Quenching Enzymes to Mitigate Microbe-Associated Problems Under Saline Conditions"

    Article Title: Discovering, Characterizing, and Applying Acyl Homoserine Lactone-Quenching Enzymes to Mitigate Microbe-Associated Problems Under Saline Conditions

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2019.00823

    AHL quenching activity comparison of eight potential protein-coding genes expressed in recombinant Escherichia coli cells. The relative AHL quenching efficiency of each sample was calculated based on Eq. (1), as stated in the “Materials and Methods” section. The E. coli TOP10 carrying vector pTricHisA and E. coli BL21 (DE3) carrying pET-20b(+) were used as negative controls. AiiA S1-5 (acyl homoserine lactonase), SDR S1-5 (oxidoreductase), Est S1-5 (esterase), and Hyd S1-5 (hydrolase) are possible AHL quenching ORFs from Altererythrobacter sp. S1-5; Hyd L11 (hydrolase), PvdQ L11 (penicillin acylase family protein), SDR L11 (oxidoreductase), and H PL11 (hypothetical protein) are possible AHL quenching ORFs from Pseudoalteromonas sp. L11. PC denotes a positive control, which is obtained by inserting an AiiA gene from Bacillus mycoides ATCC 6462 into pET-20b(+) and expressing it in E. coli BL21 (DE3). Two independent biological replicates with three technical replicates in each biological replicate were performed for this experiment.
    Figure Legend Snippet: AHL quenching activity comparison of eight potential protein-coding genes expressed in recombinant Escherichia coli cells. The relative AHL quenching efficiency of each sample was calculated based on Eq. (1), as stated in the “Materials and Methods” section. The E. coli TOP10 carrying vector pTricHisA and E. coli BL21 (DE3) carrying pET-20b(+) were used as negative controls. AiiA S1-5 (acyl homoserine lactonase), SDR S1-5 (oxidoreductase), Est S1-5 (esterase), and Hyd S1-5 (hydrolase) are possible AHL quenching ORFs from Altererythrobacter sp. S1-5; Hyd L11 (hydrolase), PvdQ L11 (penicillin acylase family protein), SDR L11 (oxidoreductase), and H PL11 (hypothetical protein) are possible AHL quenching ORFs from Pseudoalteromonas sp. L11. PC denotes a positive control, which is obtained by inserting an AiiA gene from Bacillus mycoides ATCC 6462 into pET-20b(+) and expressing it in E. coli BL21 (DE3). Two independent biological replicates with three technical replicates in each biological replicate were performed for this experiment.

    Techniques Used: Activity Assay, Recombinant, Plasmid Preparation, Positron Emission Tomography, Positive Control, Expressing

    5) Product Images from "Antimicrobial Activity of Soil Clostridium Enriched Conditioned Media Against Bacillus mycoides, Bacillus cereus, and Pseudomonas aeruginosa"

    Article Title: Antimicrobial Activity of Soil Clostridium Enriched Conditioned Media Against Bacillus mycoides, Bacillus cereus, and Pseudomonas aeruginosa

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2020.608998

    Influence of exposing Farm 4 soil conditioned medium (F4SCM) to different pH conditions on its antimicrobial activity against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM exposed to pH2 (orange), F4SCM exposed to pH4 (purple), F4SCM exposed to pH6 (green), F4SCM exposed to pH8 (brown), F4SCM exposed to pH10 (pink), F4SCM exposed to pH12 (black). Each curve represents the mean growth rate ± SD ( n = 3). Significant loss of activity was observed at high acidic (pH 2 and 4) and basic pH values (pH 10 and 12) against all three indicator microorganisms ( p
    Figure Legend Snippet: Influence of exposing Farm 4 soil conditioned medium (F4SCM) to different pH conditions on its antimicrobial activity against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM exposed to pH2 (orange), F4SCM exposed to pH4 (purple), F4SCM exposed to pH6 (green), F4SCM exposed to pH8 (brown), F4SCM exposed to pH10 (pink), F4SCM exposed to pH12 (black). Each curve represents the mean growth rate ± SD ( n = 3). Significant loss of activity was observed at high acidic (pH 2 and 4) and basic pH values (pH 10 and 12) against all three indicator microorganisms ( p

    Techniques Used: Activity Assay

    Influence of heat on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM treated at 50°C for 10 min (pink), F4SCM treated at 70°C for 10 min (black), F4SCM treated at 80°C for 10 min (orange), F4SCM treated at 90°C for 10 min (purple), and F4SCM treated at 90°C for 20 min (green) in the growth media (CMGS). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. mycoides and B. cereus was significantly decreased following heat treatment at 70°C or higher and there was no significant loss of activity against P. aeruginosa following various heat treatments ( p
    Figure Legend Snippet: Influence of heat on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM treated at 50°C for 10 min (pink), F4SCM treated at 70°C for 10 min (black), F4SCM treated at 80°C for 10 min (orange), F4SCM treated at 90°C for 10 min (purple), and F4SCM treated at 90°C for 20 min (green) in the growth media (CMGS). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. mycoides and B. cereus was significantly decreased following heat treatment at 70°C or higher and there was no significant loss of activity against P. aeruginosa following various heat treatments ( p

    Techniques Used: Activity Assay

    Involvement of Clostridium spp. isolated from Farm 4 soil in F4SCM’s antimicrobial activity. CMs were prepared from sterile Farm 4 soil (F4SCM Sterile ) and Farm 4 soil spiked with Clostridium spp. (F4SCM Spiked ) and evaluated their antimicrobial activities against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM Sterile (green), and F4SCM Spiked (blue). Each curve represents the mean growth rate ± S.D ( n = 3). F4SCM Spiked significantly inhibited the growth of all three tested microorganisms while F4SCM Sterile showed no antimicrobial activity ( p
    Figure Legend Snippet: Involvement of Clostridium spp. isolated from Farm 4 soil in F4SCM’s antimicrobial activity. CMs were prepared from sterile Farm 4 soil (F4SCM Sterile ) and Farm 4 soil spiked with Clostridium spp. (F4SCM Spiked ) and evaluated their antimicrobial activities against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM Sterile (green), and F4SCM Spiked (blue). Each curve represents the mean growth rate ± S.D ( n = 3). F4SCM Spiked significantly inhibited the growth of all three tested microorganisms while F4SCM Sterile showed no antimicrobial activity ( p

    Techniques Used: Isolation, Activity Assay

    Effect of protease enzyme on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B), and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), and F4SCM treated with 1 mg/mL protease enzyme (green). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. cereus significantly changed after protease enzyme treatment (1 mg/mL) and there was no significant effect against B. mycoides and P. aeruginosa after protease enzyme treatment ( p
    Figure Legend Snippet: Effect of protease enzyme on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B), and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), and F4SCM treated with 1 mg/mL protease enzyme (green). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. cereus significantly changed after protease enzyme treatment (1 mg/mL) and there was no significant effect against B. mycoides and P. aeruginosa after protease enzyme treatment ( p

    Techniques Used: Activity Assay

    Effect of five soil conditioned media on the growth of B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F1SCM (Farm 1 soil conditioned media; blue), F2SCM (Farm 2 soil conditioned media; pink), F3SCM (Farm 3 soil conditioned media; black), F4SCM (Farm 4 soil conditioned media; orange), F5SCM (Farm 5 soil conditioned media; purple), and nisin (green) in the growth media (CMGS). Nisin (45 μM) and butterfield’s diluent served as positive and untreated control, respectively. Each curve represents the mean growth ± SD ( n = 3). All treatments were significantly different from untreated control ( p
    Figure Legend Snippet: Effect of five soil conditioned media on the growth of B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F1SCM (Farm 1 soil conditioned media; blue), F2SCM (Farm 2 soil conditioned media; pink), F3SCM (Farm 3 soil conditioned media; black), F4SCM (Farm 4 soil conditioned media; orange), F5SCM (Farm 5 soil conditioned media; purple), and nisin (green) in the growth media (CMGS). Nisin (45 μM) and butterfield’s diluent served as positive and untreated control, respectively. Each curve represents the mean growth ± SD ( n = 3). All treatments were significantly different from untreated control ( p

    Techniques Used:

    6) Product Images from "Antimicrobial Activity of Soil Clostridium Enriched Conditioned Media Against Bacillus mycoides, Bacillus cereus, and Pseudomonas aeruginosa"

    Article Title: Antimicrobial Activity of Soil Clostridium Enriched Conditioned Media Against Bacillus mycoides, Bacillus cereus, and Pseudomonas aeruginosa

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2020.608998

    Influence of exposing Farm 4 soil conditioned medium (F4SCM) to different pH conditions on its antimicrobial activity against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM exposed to pH2 (orange), F4SCM exposed to pH4 (purple), F4SCM exposed to pH6 (green), F4SCM exposed to pH8 (brown), F4SCM exposed to pH10 (pink), F4SCM exposed to pH12 (black). Each curve represents the mean growth rate ± SD ( n = 3). Significant loss of activity was observed at high acidic (pH 2 and 4) and basic pH values (pH 10 and 12) against all three indicator microorganisms ( p
    Figure Legend Snippet: Influence of exposing Farm 4 soil conditioned medium (F4SCM) to different pH conditions on its antimicrobial activity against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM exposed to pH2 (orange), F4SCM exposed to pH4 (purple), F4SCM exposed to pH6 (green), F4SCM exposed to pH8 (brown), F4SCM exposed to pH10 (pink), F4SCM exposed to pH12 (black). Each curve represents the mean growth rate ± SD ( n = 3). Significant loss of activity was observed at high acidic (pH 2 and 4) and basic pH values (pH 10 and 12) against all three indicator microorganisms ( p

    Techniques Used: Activity Assay

    Influence of heat on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM treated at 50°C for 10 min (pink), F4SCM treated at 70°C for 10 min (black), F4SCM treated at 80°C for 10 min (orange), F4SCM treated at 90°C for 10 min (purple), and F4SCM treated at 90°C for 20 min (green) in the growth media (CMGS). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. mycoides and B. cereus was significantly decreased following heat treatment at 70°C or higher and there was no significant loss of activity against P. aeruginosa following various heat treatments ( p
    Figure Legend Snippet: Influence of heat on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM treated at 50°C for 10 min (pink), F4SCM treated at 70°C for 10 min (black), F4SCM treated at 80°C for 10 min (orange), F4SCM treated at 90°C for 10 min (purple), and F4SCM treated at 90°C for 20 min (green) in the growth media (CMGS). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. mycoides and B. cereus was significantly decreased following heat treatment at 70°C or higher and there was no significant loss of activity against P. aeruginosa following various heat treatments ( p

    Techniques Used: Activity Assay

    Involvement of Clostridium spp. isolated from Farm 4 soil in F4SCM’s antimicrobial activity. CMs were prepared from sterile Farm 4 soil (F4SCM Sterile ) and Farm 4 soil spiked with Clostridium spp. (F4SCM Spiked ) and evaluated their antimicrobial activities against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM Sterile (green), and F4SCM Spiked (blue). Each curve represents the mean growth rate ± S.D ( n = 3). F4SCM Spiked significantly inhibited the growth of all three tested microorganisms while F4SCM Sterile showed no antimicrobial activity ( p
    Figure Legend Snippet: Involvement of Clostridium spp. isolated from Farm 4 soil in F4SCM’s antimicrobial activity. CMs were prepared from sterile Farm 4 soil (F4SCM Sterile ) and Farm 4 soil spiked with Clostridium spp. (F4SCM Spiked ) and evaluated their antimicrobial activities against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM Sterile (green), and F4SCM Spiked (blue). Each curve represents the mean growth rate ± S.D ( n = 3). F4SCM Spiked significantly inhibited the growth of all three tested microorganisms while F4SCM Sterile showed no antimicrobial activity ( p

    Techniques Used: Isolation, Activity Assay

    Effect of protease enzyme on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B), and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), and F4SCM treated with 1 mg/mL protease enzyme (green). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. cereus significantly changed after protease enzyme treatment (1 mg/mL) and there was no significant effect against B. mycoides and P. aeruginosa after protease enzyme treatment ( p
    Figure Legend Snippet: Effect of protease enzyme on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B), and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), and F4SCM treated with 1 mg/mL protease enzyme (green). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. cereus significantly changed after protease enzyme treatment (1 mg/mL) and there was no significant effect against B. mycoides and P. aeruginosa after protease enzyme treatment ( p

    Techniques Used: Activity Assay

    Effect of five soil conditioned media on the growth of B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F1SCM (Farm 1 soil conditioned media; blue), F2SCM (Farm 2 soil conditioned media; pink), F3SCM (Farm 3 soil conditioned media; black), F4SCM (Farm 4 soil conditioned media; orange), F5SCM (Farm 5 soil conditioned media; purple), and nisin (green) in the growth media (CMGS). Nisin (45 μM) and butterfield’s diluent served as positive and untreated control, respectively. Each curve represents the mean growth ± SD ( n = 3). All treatments were significantly different from untreated control ( p
    Figure Legend Snippet: Effect of five soil conditioned media on the growth of B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F1SCM (Farm 1 soil conditioned media; blue), F2SCM (Farm 2 soil conditioned media; pink), F3SCM (Farm 3 soil conditioned media; black), F4SCM (Farm 4 soil conditioned media; orange), F5SCM (Farm 5 soil conditioned media; purple), and nisin (green) in the growth media (CMGS). Nisin (45 μM) and butterfield’s diluent served as positive and untreated control, respectively. Each curve represents the mean growth ± SD ( n = 3). All treatments were significantly different from untreated control ( p

    Techniques Used:

    7) Product Images from "Genomic Characterization and Probiotic Potency of Bacillus sp. DU-106, a Highly Effective Producer of L-Lactic Acid Isolated From Fermented Yogurt"

    Article Title: Genomic Characterization and Probiotic Potency of Bacillus sp. DU-106, a Highly Effective Producer of L-Lactic Acid Isolated From Fermented Yogurt

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2018.02216

    Comparative genome analyses of Bacillus sp. DU-106 and other Bacillus strains. Venn diagram of the genome comparison of Bacillus sp. DU-106 with other B. cereus strains (A) Venn diagram displays the orthologous genes between B. cereus ATCC 14579, B. thuringiensis ATCC 10792, B. toyonensis BCT-7112, and B. mycoides ATCC 6462. The maximum likelihood phylogenetic tree was constructed with PhyML based on SNP differences across the whole genome (B) Bootstrap support values were calculated from 100 replicates.
    Figure Legend Snippet: Comparative genome analyses of Bacillus sp. DU-106 and other Bacillus strains. Venn diagram of the genome comparison of Bacillus sp. DU-106 with other B. cereus strains (A) Venn diagram displays the orthologous genes between B. cereus ATCC 14579, B. thuringiensis ATCC 10792, B. toyonensis BCT-7112, and B. mycoides ATCC 6462. The maximum likelihood phylogenetic tree was constructed with PhyML based on SNP differences across the whole genome (B) Bootstrap support values were calculated from 100 replicates.

    Techniques Used: Construct

    8) Product Images from "Antimicrobial Activity of Soil Clostridium Enriched Conditioned Media Against Bacillus mycoides, Bacillus cereus, and Pseudomonas aeruginosa"

    Article Title: Antimicrobial Activity of Soil Clostridium Enriched Conditioned Media Against Bacillus mycoides, Bacillus cereus, and Pseudomonas aeruginosa

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2020.608998

    Influence of exposing Farm 4 soil conditioned medium (F4SCM) to different pH conditions on its antimicrobial activity against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM exposed to pH2 (orange), F4SCM exposed to pH4 (purple), F4SCM exposed to pH6 (green), F4SCM exposed to pH8 (brown), F4SCM exposed to pH10 (pink), F4SCM exposed to pH12 (black). Each curve represents the mean growth rate ± SD ( n = 3). Significant loss of activity was observed at high acidic (pH 2 and 4) and basic pH values (pH 10 and 12) against all three indicator microorganisms ( p
    Figure Legend Snippet: Influence of exposing Farm 4 soil conditioned medium (F4SCM) to different pH conditions on its antimicrobial activity against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM exposed to pH2 (orange), F4SCM exposed to pH4 (purple), F4SCM exposed to pH6 (green), F4SCM exposed to pH8 (brown), F4SCM exposed to pH10 (pink), F4SCM exposed to pH12 (black). Each curve represents the mean growth rate ± SD ( n = 3). Significant loss of activity was observed at high acidic (pH 2 and 4) and basic pH values (pH 10 and 12) against all three indicator microorganisms ( p

    Techniques Used: Activity Assay

    Influence of heat on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM treated at 50°C for 10 min (pink), F4SCM treated at 70°C for 10 min (black), F4SCM treated at 80°C for 10 min (orange), F4SCM treated at 90°C for 10 min (purple), and F4SCM treated at 90°C for 20 min (green) in the growth media (CMGS). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. mycoides and B. cereus was significantly decreased following heat treatment at 70°C or higher and there was no significant loss of activity against P. aeruginosa following various heat treatments ( p
    Figure Legend Snippet: Influence of heat on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM treated at 50°C for 10 min (pink), F4SCM treated at 70°C for 10 min (black), F4SCM treated at 80°C for 10 min (orange), F4SCM treated at 90°C for 10 min (purple), and F4SCM treated at 90°C for 20 min (green) in the growth media (CMGS). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. mycoides and B. cereus was significantly decreased following heat treatment at 70°C or higher and there was no significant loss of activity against P. aeruginosa following various heat treatments ( p

    Techniques Used: Activity Assay

    Involvement of Clostridium spp. isolated from Farm 4 soil in F4SCM’s antimicrobial activity. CMs were prepared from sterile Farm 4 soil (F4SCM Sterile ) and Farm 4 soil spiked with Clostridium spp. (F4SCM Spiked ) and evaluated their antimicrobial activities against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM Sterile (green), and F4SCM Spiked (blue). Each curve represents the mean growth rate ± S.D ( n = 3). F4SCM Spiked significantly inhibited the growth of all three tested microorganisms while F4SCM Sterile showed no antimicrobial activity ( p
    Figure Legend Snippet: Involvement of Clostridium spp. isolated from Farm 4 soil in F4SCM’s antimicrobial activity. CMs were prepared from sterile Farm 4 soil (F4SCM Sterile ) and Farm 4 soil spiked with Clostridium spp. (F4SCM Spiked ) and evaluated their antimicrobial activities against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM Sterile (green), and F4SCM Spiked (blue). Each curve represents the mean growth rate ± S.D ( n = 3). F4SCM Spiked significantly inhibited the growth of all three tested microorganisms while F4SCM Sterile showed no antimicrobial activity ( p

    Techniques Used: Isolation, Activity Assay

    Effect of protease enzyme on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B), and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), and F4SCM treated with 1 mg/mL protease enzyme (green). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. cereus significantly changed after protease enzyme treatment (1 mg/mL) and there was no significant effect against B. mycoides and P. aeruginosa after protease enzyme treatment ( p
    Figure Legend Snippet: Effect of protease enzyme on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B), and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), and F4SCM treated with 1 mg/mL protease enzyme (green). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. cereus significantly changed after protease enzyme treatment (1 mg/mL) and there was no significant effect against B. mycoides and P. aeruginosa after protease enzyme treatment ( p

    Techniques Used: Activity Assay

    Effect of five soil conditioned media on the growth of B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F1SCM (Farm 1 soil conditioned media; blue), F2SCM (Farm 2 soil conditioned media; pink), F3SCM (Farm 3 soil conditioned media; black), F4SCM (Farm 4 soil conditioned media; orange), F5SCM (Farm 5 soil conditioned media; purple), and nisin (green) in the growth media (CMGS). Nisin (45 μM) and butterfield’s diluent served as positive and untreated control, respectively. Each curve represents the mean growth ± SD ( n = 3). All treatments were significantly different from untreated control ( p
    Figure Legend Snippet: Effect of five soil conditioned media on the growth of B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F1SCM (Farm 1 soil conditioned media; blue), F2SCM (Farm 2 soil conditioned media; pink), F3SCM (Farm 3 soil conditioned media; black), F4SCM (Farm 4 soil conditioned media; orange), F5SCM (Farm 5 soil conditioned media; purple), and nisin (green) in the growth media (CMGS). Nisin (45 μM) and butterfield’s diluent served as positive and untreated control, respectively. Each curve represents the mean growth ± SD ( n = 3). All treatments were significantly different from untreated control ( p

    Techniques Used:

    9) Product Images from "Raman Spectroscopy-Compatible Inactivation Method for Pathogenic Endospores ▿"

    Article Title: Raman Spectroscopy-Compatible Inactivation Method for Pathogenic Endospores ▿

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.02481-09

    Baseline-corrected mean Raman spectra, each calculated from 20 single-endospore spectra of B. mycoides DSM 299. Shown are spectra for viable, untreated spores (A) and spores inactivated via autoclaving (B), as well as a difference spectrum (A-B).
    Figure Legend Snippet: Baseline-corrected mean Raman spectra, each calculated from 20 single-endospore spectra of B. mycoides DSM 299. Shown are spectra for viable, untreated spores (A) and spores inactivated via autoclaving (B), as well as a difference spectrum (A-B).

    Techniques Used:

    10) Product Images from "Genomic Characterization and Probiotic Potency of Bacillus sp. DU-106, a Highly Effective Producer of L-Lactic Acid Isolated From Fermented Yogurt"

    Article Title: Genomic Characterization and Probiotic Potency of Bacillus sp. DU-106, a Highly Effective Producer of L-Lactic Acid Isolated From Fermented Yogurt

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2018.02216

    Comparative genome analyses of Bacillus sp. DU-106 and other Bacillus strains. Venn diagram of the genome comparison of Bacillus sp. DU-106 with other B. cereus strains (A) Venn diagram displays the orthologous genes between B. cereus ATCC 14579, B. thuringiensis ATCC 10792, B. toyonensis BCT-7112, and B. mycoides ATCC 6462. The maximum likelihood phylogenetic tree was constructed with PhyML based on SNP differences across the whole genome (B) Bootstrap support values were calculated from 100 replicates.
    Figure Legend Snippet: Comparative genome analyses of Bacillus sp. DU-106 and other Bacillus strains. Venn diagram of the genome comparison of Bacillus sp. DU-106 with other B. cereus strains (A) Venn diagram displays the orthologous genes between B. cereus ATCC 14579, B. thuringiensis ATCC 10792, B. toyonensis BCT-7112, and B. mycoides ATCC 6462. The maximum likelihood phylogenetic tree was constructed with PhyML based on SNP differences across the whole genome (B) Bootstrap support values were calculated from 100 replicates.

    Techniques Used: Construct

    11) Product Images from "Antimicrobial Activity of Soil Clostridium Enriched Conditioned Media Against Bacillus mycoides, Bacillus cereus, and Pseudomonas aeruginosa"

    Article Title: Antimicrobial Activity of Soil Clostridium Enriched Conditioned Media Against Bacillus mycoides, Bacillus cereus, and Pseudomonas aeruginosa

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2020.608998

    Influence of exposing Farm 4 soil conditioned medium (F4SCM) to different pH conditions on its antimicrobial activity against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM exposed to pH2 (orange), F4SCM exposed to pH4 (purple), F4SCM exposed to pH6 (green), F4SCM exposed to pH8 (brown), F4SCM exposed to pH10 (pink), F4SCM exposed to pH12 (black). Each curve represents the mean growth rate ± SD ( n = 3). Significant loss of activity was observed at high acidic (pH 2 and 4) and basic pH values (pH 10 and 12) against all three indicator microorganisms ( p
    Figure Legend Snippet: Influence of exposing Farm 4 soil conditioned medium (F4SCM) to different pH conditions on its antimicrobial activity against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM exposed to pH2 (orange), F4SCM exposed to pH4 (purple), F4SCM exposed to pH6 (green), F4SCM exposed to pH8 (brown), F4SCM exposed to pH10 (pink), F4SCM exposed to pH12 (black). Each curve represents the mean growth rate ± SD ( n = 3). Significant loss of activity was observed at high acidic (pH 2 and 4) and basic pH values (pH 10 and 12) against all three indicator microorganisms ( p

    Techniques Used: Activity Assay

    Influence of heat on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM treated at 50°C for 10 min (pink), F4SCM treated at 70°C for 10 min (black), F4SCM treated at 80°C for 10 min (orange), F4SCM treated at 90°C for 10 min (purple), and F4SCM treated at 90°C for 20 min (green) in the growth media (CMGS). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. mycoides and B. cereus was significantly decreased following heat treatment at 70°C or higher and there was no significant loss of activity against P. aeruginosa following various heat treatments ( p
    Figure Legend Snippet: Influence of heat on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), F4SCM treated at 50°C for 10 min (pink), F4SCM treated at 70°C for 10 min (black), F4SCM treated at 80°C for 10 min (orange), F4SCM treated at 90°C for 10 min (purple), and F4SCM treated at 90°C for 20 min (green) in the growth media (CMGS). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. mycoides and B. cereus was significantly decreased following heat treatment at 70°C or higher and there was no significant loss of activity against P. aeruginosa following various heat treatments ( p

    Techniques Used: Activity Assay

    Involvement of Clostridium spp. isolated from Farm 4 soil in F4SCM’s antimicrobial activity. CMs were prepared from sterile Farm 4 soil (F4SCM Sterile ) and Farm 4 soil spiked with Clostridium spp. (F4SCM Spiked ) and evaluated their antimicrobial activities against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM Sterile (green), and F4SCM Spiked (blue). Each curve represents the mean growth rate ± S.D ( n = 3). F4SCM Spiked significantly inhibited the growth of all three tested microorganisms while F4SCM Sterile showed no antimicrobial activity ( p
    Figure Legend Snippet: Involvement of Clostridium spp. isolated from Farm 4 soil in F4SCM’s antimicrobial activity. CMs were prepared from sterile Farm 4 soil (F4SCM Sterile ) and Farm 4 soil spiked with Clostridium spp. (F4SCM Spiked ) and evaluated their antimicrobial activities against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM Sterile (green), and F4SCM Spiked (blue). Each curve represents the mean growth rate ± S.D ( n = 3). F4SCM Spiked significantly inhibited the growth of all three tested microorganisms while F4SCM Sterile showed no antimicrobial activity ( p

    Techniques Used: Isolation, Activity Assay

    Effect of protease enzyme on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B), and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), and F4SCM treated with 1 mg/mL protease enzyme (green). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. cereus significantly changed after protease enzyme treatment (1 mg/mL) and there was no significant effect against B. mycoides and P. aeruginosa after protease enzyme treatment ( p
    Figure Legend Snippet: Effect of protease enzyme on the antimicrobial activity of Farm 4 soil conditioned medium (F4SCM) against B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B), and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F4SCM (blue), and F4SCM treated with 1 mg/mL protease enzyme (green). Each curve represents the mean growth rate ± S.D ( n = 3). Antimicrobial activity against B. cereus significantly changed after protease enzyme treatment (1 mg/mL) and there was no significant effect against B. mycoides and P. aeruginosa after protease enzyme treatment ( p

    Techniques Used: Activity Assay

    Effect of five soil conditioned media on the growth of B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F1SCM (Farm 1 soil conditioned media; blue), F2SCM (Farm 2 soil conditioned media; pink), F3SCM (Farm 3 soil conditioned media; black), F4SCM (Farm 4 soil conditioned media; orange), F5SCM (Farm 5 soil conditioned media; purple), and nisin (green) in the growth media (CMGS). Nisin (45 μM) and butterfield’s diluent served as positive and untreated control, respectively. Each curve represents the mean growth ± SD ( n = 3). All treatments were significantly different from untreated control ( p
    Figure Legend Snippet: Effect of five soil conditioned media on the growth of B. mycoides ATCC 6462 (A) , B. cereus NZRM 5 (B) , and P. aeruginosa ATCC 25668 (C) . Bacteria were grown in the presence of butterfield’s diluent (red), F1SCM (Farm 1 soil conditioned media; blue), F2SCM (Farm 2 soil conditioned media; pink), F3SCM (Farm 3 soil conditioned media; black), F4SCM (Farm 4 soil conditioned media; orange), F5SCM (Farm 5 soil conditioned media; purple), and nisin (green) in the growth media (CMGS). Nisin (45 μM) and butterfield’s diluent served as positive and untreated control, respectively. Each curve represents the mean growth ± SD ( n = 3). All treatments were significantly different from untreated control ( p

    Techniques Used:

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  • 93
    ATCC b mycoides type atcc 6462 strain
    Ribotyping in Bacillus strains. A blot of EcoRI digested total DNA was hybridized by a probe specific for an evolutionarily conserved region of 16S rRNA. Various members of the B. cereus group show different patterns: lane 1, B. <t>mycoides</t> <t>ATCC</t> 6462 T ; lane 2, B. mycoides DX; lane 3, B. pseudomycoides NRRL B-617 T ; lane 4, B. mycoides NRRL NRS-273; lane 5, B. thuringiensis BGSC 4D; lane 6, B. mycoides SIN. Mutant strains all derived from SIN96, with identical patterns: lane 7, B. mycoides SINett; lane 8, CAD; lane 9, CIC; lane 10–13 cotton-like mutants.
    B Mycoides Type Atcc 6462 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b mycoides type atcc 6462 strain/product/ATCC
    Average 93 stars, based on 3 article reviews
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    b mycoides type atcc 6462 strain - by Bioz Stars, 2022-09
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    93
    ATCC b mycoides atcc 6462
    Comparative genome analyses of Bacillus sp. DU-106 and other Bacillus strains. Venn diagram of the genome comparison of Bacillus sp. DU-106 with other B. cereus strains (A) Venn diagram displays the orthologous genes between B. cereus ATCC 14579, B. thuringiensis ATCC 10792, B. toyonensis BCT-7112, and B. <t>mycoides</t> ATCC 6462. The maximum likelihood phylogenetic tree was constructed with PhyML based on SNP differences across the whole genome (B) Bootstrap support values were calculated from 100 replicates.
    B Mycoides Atcc 6462, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b mycoides atcc 6462/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    b mycoides atcc 6462 - by Bioz Stars, 2022-09
    93/100 stars
      Buy from Supplier

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    Ribotyping in Bacillus strains. A blot of EcoRI digested total DNA was hybridized by a probe specific for an evolutionarily conserved region of 16S rRNA. Various members of the B. cereus group show different patterns: lane 1, B. mycoides ATCC 6462 T ; lane 2, B. mycoides DX; lane 3, B. pseudomycoides NRRL B-617 T ; lane 4, B. mycoides NRRL NRS-273; lane 5, B. thuringiensis BGSC 4D; lane 6, B. mycoides SIN. Mutant strains all derived from SIN96, with identical patterns: lane 7, B. mycoides SINett; lane 8, CAD; lane 9, CIC; lane 10–13 cotton-like mutants.

    Journal: BMC Microbiology

    Article Title: Colony shape as a genetic trait in the pattern-forming Bacillus mycoides

    doi: 10.1186/1471-2180-2-33

    Figure Lengend Snippet: Ribotyping in Bacillus strains. A blot of EcoRI digested total DNA was hybridized by a probe specific for an evolutionarily conserved region of 16S rRNA. Various members of the B. cereus group show different patterns: lane 1, B. mycoides ATCC 6462 T ; lane 2, B. mycoides DX; lane 3, B. pseudomycoides NRRL B-617 T ; lane 4, B. mycoides NRRL NRS-273; lane 5, B. thuringiensis BGSC 4D; lane 6, B. mycoides SIN. Mutant strains all derived from SIN96, with identical patterns: lane 7, B. mycoides SINett; lane 8, CAD; lane 9, CIC; lane 10–13 cotton-like mutants.

    Article Snippet: The same spacer region was analyzed in B. mycoides Type ATCC 6462 strain and in B. pseudomycoides Type B617 strain [ ].

    Techniques: Mutagenesis, Derivative Assay

    Phase contrast and fluorescence microscopy visualization of non-permebilized: ( A ) free floating E. coli and ( B ) aggregates of B. mycoides . Control— E. coli BL21DE3 and B. mycoides incubated without dyes, GFP— E. coli BL21DE3 producing GFP protein, 5l and 6q — E. coli BL21DE3 and B. mycoides stained with the appropriate dye, PC—phase-contrast microscopy and UV—fluorescence microscopy. Scale bars correspond to 20 µm.

    Journal: Scientific Reports

    Article Title: Development of Safirinium dyes for new applications: fluorescent staining of bacteria, human kidney cells, and the horny layer of the epidermis

    doi: 10.1038/s41598-022-19262-w

    Figure Lengend Snippet: Phase contrast and fluorescence microscopy visualization of non-permebilized: ( A ) free floating E. coli and ( B ) aggregates of B. mycoides . Control— E. coli BL21DE3 and B. mycoides incubated without dyes, GFP— E. coli BL21DE3 producing GFP protein, 5l and 6q — E. coli BL21DE3 and B. mycoides stained with the appropriate dye, PC—phase-contrast microscopy and UV—fluorescence microscopy. Scale bars correspond to 20 µm.

    Article Snippet: The following bacterial strains were used: E. coli BL21DE3 (Merck), E. coli BL21DE3 encoding pSFOXB20-daGFP plasmid (Oxford Genetics) for constitutive production of GFP (Green Fluorescent Protein) and Bacillus mycoides (ATCC 6462).

    Techniques: Fluorescence, Microscopy, Incubation, Staining

    AHL quenching activity comparison of eight potential protein-coding genes expressed in recombinant Escherichia coli cells. The relative AHL quenching efficiency of each sample was calculated based on Eq. (1), as stated in the “Materials and Methods” section. The E. coli TOP10 carrying vector pTricHisA and E. coli BL21 (DE3) carrying pET-20b(+) were used as negative controls. AiiA S1-5 (acyl homoserine lactonase), SDR S1-5 (oxidoreductase), Est S1-5 (esterase), and Hyd S1-5 (hydrolase) are possible AHL quenching ORFs from Altererythrobacter sp. S1-5; Hyd L11 (hydrolase), PvdQ L11 (penicillin acylase family protein), SDR L11 (oxidoreductase), and H PL11 (hypothetical protein) are possible AHL quenching ORFs from Pseudoalteromonas sp. L11. PC denotes a positive control, which is obtained by inserting an AiiA gene from Bacillus mycoides ATCC 6462 into pET-20b(+) and expressing it in E. coli BL21 (DE3). Two independent biological replicates with three technical replicates in each biological replicate were performed for this experiment.

    Journal: Frontiers in Microbiology

    Article Title: Discovering, Characterizing, and Applying Acyl Homoserine Lactone-Quenching Enzymes to Mitigate Microbe-Associated Problems Under Saline Conditions

    doi: 10.3389/fmicb.2019.00823

    Figure Lengend Snippet: AHL quenching activity comparison of eight potential protein-coding genes expressed in recombinant Escherichia coli cells. The relative AHL quenching efficiency of each sample was calculated based on Eq. (1), as stated in the “Materials and Methods” section. The E. coli TOP10 carrying vector pTricHisA and E. coli BL21 (DE3) carrying pET-20b(+) were used as negative controls. AiiA S1-5 (acyl homoserine lactonase), SDR S1-5 (oxidoreductase), Est S1-5 (esterase), and Hyd S1-5 (hydrolase) are possible AHL quenching ORFs from Altererythrobacter sp. S1-5; Hyd L11 (hydrolase), PvdQ L11 (penicillin acylase family protein), SDR L11 (oxidoreductase), and H PL11 (hypothetical protein) are possible AHL quenching ORFs from Pseudoalteromonas sp. L11. PC denotes a positive control, which is obtained by inserting an AiiA gene from Bacillus mycoides ATCC 6462 into pET-20b(+) and expressing it in E. coli BL21 (DE3). Two independent biological replicates with three technical replicates in each biological replicate were performed for this experiment.

    Article Snippet: The positive control made up of AiiA from B. mycoides ATCC 6462 showed a 37.9% relative AHL QE compared with the negative control.

    Techniques: Activity Assay, Recombinant, Plasmid Preparation, Positron Emission Tomography, Positive Control, Expressing

    Comparative genome analyses of Bacillus sp. DU-106 and other Bacillus strains. Venn diagram of the genome comparison of Bacillus sp. DU-106 with other B. cereus strains (A) Venn diagram displays the orthologous genes between B. cereus ATCC 14579, B. thuringiensis ATCC 10792, B. toyonensis BCT-7112, and B. mycoides ATCC 6462. The maximum likelihood phylogenetic tree was constructed with PhyML based on SNP differences across the whole genome (B) Bootstrap support values were calculated from 100 replicates.

    Journal: Frontiers in Microbiology

    Article Title: Genomic Characterization and Probiotic Potency of Bacillus sp. DU-106, a Highly Effective Producer of L-Lactic Acid Isolated From Fermented Yogurt

    doi: 10.3389/fmicb.2018.02216

    Figure Lengend Snippet: Comparative genome analyses of Bacillus sp. DU-106 and other Bacillus strains. Venn diagram of the genome comparison of Bacillus sp. DU-106 with other B. cereus strains (A) Venn diagram displays the orthologous genes between B. cereus ATCC 14579, B. thuringiensis ATCC 10792, B. toyonensis BCT-7112, and B. mycoides ATCC 6462. The maximum likelihood phylogenetic tree was constructed with PhyML based on SNP differences across the whole genome (B) Bootstrap support values were calculated from 100 replicates.

    Article Snippet: The strain DU-106 showed a high synteny of 92.39% with B. cereus ATCC 14579, 78.12% with B. thuringiensis ATCC 10792, 88.14% with B. toyonensis BCT-7112, 80.19% with B. mycoides ATCC 6462, and 64.59% with B. pseudomycoides DSM 12442, but displayed a low synteny of 4.82% with B. coagulans ATCC 7050 ( Supplementary Figure ).

    Techniques: Construct