Journal:

Article Title: Analysis of the Sulfate-Reducing Bacterial and Methanogenic Archaeal Populations in Contrasting Antarctic Sediments

doi: 10.1128/AEM.69.6.3181-3191.2003

Figure Lengend Snippet: 16S rRNA-targeted oligonucleotide probes and pure-culture controls used in probing experiments with RNAs extracted from sediments from two contrasting sites on Signy Island, Antarctica a

Article Snippet: 16S rRNA controls for Desulfotalea and the methanogens studied in this work (Table ) were transcribed in vitro from cloned 16S rRNA genes ( 46 ). table ft1 table-wrap mode="anchored" t5 TABLE 2. caption a7 Probe b Target group Pure-culture controls Reference or source p338 (S-D-Bact-0338-a-A-18) Most bacteria SRBs, Bacillus subtilis NCMB 3610 3 p385 (S-∗-SRB-0385-a-A-18) δ- Proteobacteria SRBs 3 p687 (S-G-Dsv-0687-a-A-16) Desulfovibrionaceae Desulfovibrio vulgaris DSM 644 15 p804 (S-∗-Dsb-0804-a-A-18) Desulfobacteriaceae Desulfobacter latus , Desulfobacterium autotrophicum 15 p129 (S-G-Dsb-0129-a-A-18) Desulfobacter Desulfobacter latus ATCC 43918 15 p221 (S-G-Dsbm-0221-a-A-20) Desulfobacterium or Desulfobacula Desulfobacterium autotrophicum DSM 3382 15 Sval 428 (S-G-Dstl-0428-a-A-19) Desulfotalea / Desulforhopalus cluster Desulfotalea psychrophila DSM 12343 (in vitro RNA) 56 p660 (S-G-Dsbb-0660-a-A-20) Desulfobulbus Desulfobulbus propionicus DSM 2056 15 DFM228 (S-G-Dtm-0228-a-A-15) Desulfotomaculum Desulfotomaculum sp. 12 p915 (S-D-Arch-0915-a-A-18) All Archaea All methanogens (in vitro RNA) 2 MG1200 (S-O-Mmic-1200-a-A-21) Methanomicrobiales Methanogenium (in vitro RNA) 52 Mgn74 c (S-G-Mgn-0074-a-A-21) Methanogenium Methanogenium (in vitro RNA) This study MSMX860 (S-O-Msar-0860-a-A-21) Methanosarcinales Methanosaeta (in vitro RNA) 52 Mst825 (S-G-Mst-0825-a-A-21) Methanosaeta Methanosaeta (in vitro RNA) 52 Mcc948 d (S-G-Mcc-0948-a-A-20) Methanococcoides Methanococcoides (in vitro RNA) This study Open in a separate window a SRB cultures were grown as described by Widdel and Bak ( 68 ).

Techniques: In Vitro