spiramycin  (ATCC)


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    ATCC spiramycin
    Distribution of macrolide resistance genes and MICs of erythromycin (A), azithromycin (B), tilmicosin (C), tylosin (D), and <t>spiramycin</t> (E) in 62 genome-sequenced MDR E. coli isolates from pigs in Denmark. The dashed line indicates the tentative epidemiological cutoff determined by ECOFFinder. WT, wild-type population.
    Spiramycin, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spiramycin/product/ATCC
    Average 86 stars, based on 1 article reviews
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    spiramycin - by Bioz Stars, 2023-06
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    1) Product Images from "Macrolide Resistance and In Vitro Potentiation by Peptidomimetics in Porcine Clinical Escherichia coli"

    Article Title: Macrolide Resistance and In Vitro Potentiation by Peptidomimetics in Porcine Clinical Escherichia coli

    Journal: mSphere

    doi: 10.1128/msphere.00402-22

    Distribution of macrolide resistance genes and MICs of erythromycin (A), azithromycin (B), tilmicosin (C), tylosin (D), and spiramycin (E) in 62 genome-sequenced MDR E. coli isolates from pigs in Denmark. The dashed line indicates the tentative epidemiological cutoff determined by ECOFFinder. WT, wild-type population.
    Figure Legend Snippet: Distribution of macrolide resistance genes and MICs of erythromycin (A), azithromycin (B), tilmicosin (C), tylosin (D), and spiramycin (E) in 62 genome-sequenced MDR E. coli isolates from pigs in Denmark. The dashed line indicates the tentative epidemiological cutoff determined by ECOFFinder. WT, wild-type population.

    Techniques Used:

    spiramycin  (ATCC)


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    ATCC spiramycin
    Growth curves and antibiotic production in S. ambofaciens ATCC 23877 (w.t.), and derivative ΩpirA, and ΩpirA rpoB(R) mutants. ( a – d ) Strains were cultivated in either YS ( a , b ) or SFM ( c , d ) broths, and biomass ( a , c ), <t>spiramycin</t> ( b ), and antimycin ( d ) values were determined at different time points. The values were calculated as the mean of three or more experiments. The errors bar indicates the standard deviation.
    Spiramycin, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spiramycin/product/ATCC
    Average 86 stars, based on 1 article reviews
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    spiramycin - by Bioz Stars, 2023-06
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    1) Product Images from "Interplay between Non-Coding RNA Transcription, Stringent/Relaxed Phenotype and Antibiotic Production in Streptomyces ambofaciens"

    Article Title: Interplay between Non-Coding RNA Transcription, Stringent/Relaxed Phenotype and Antibiotic Production in Streptomyces ambofaciens

    Journal: Antibiotics

    doi: 10.3390/antibiotics10080947

    Growth curves and antibiotic production in S. ambofaciens ATCC 23877 (w.t.), and derivative ΩpirA, and ΩpirA rpoB(R) mutants. ( a – d ) Strains were cultivated in either YS ( a , b ) or SFM ( c , d ) broths, and biomass ( a , c ), spiramycin ( b ), and antimycin ( d ) values were determined at different time points. The values were calculated as the mean of three or more experiments. The errors bar indicates the standard deviation.
    Figure Legend Snippet: Growth curves and antibiotic production in S. ambofaciens ATCC 23877 (w.t.), and derivative ΩpirA, and ΩpirA rpoB(R) mutants. ( a – d ) Strains were cultivated in either YS ( a , b ) or SFM ( c , d ) broths, and biomass ( a , c ), spiramycin ( b ), and antimycin ( d ) values were determined at different time points. The values were calculated as the mean of three or more experiments. The errors bar indicates the standard deviation.

    Techniques Used: Standard Deviation

    spiramycin  (ATCC)


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    ATCC spiramycin
    Spiramycin, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spiramycin/product/ATCC
    Average 86 stars, based on 1 article reviews
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    spiramycin  (ATCC)


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    ATCC spiramycin
    The terminal inverted repeats (TIRs, 200 kb) and the origin of replication ( oriC ) are indicated in black. The distribution of gene presenting features of interest are represented in blue or red when enriched in the central region or within the extremities (defined by the first and last rDNA operons), respectively (see Supplementary Table 3 for a statistical analysis of the data). The dot and asterisk indicate the position of pSAM2 and of a complete prophage, respectively. The position of the SMBGC encoding the biosynthesis of all known antibacterial compounds of S. ambofaciens ATCC 23877 is also indicated. The level of synteny along the chromosome of S. ambofaciens ATCC 23877 is represented as a heat map of GOC (Gene Order Conservation) scores using a sliding window (8 CDSs with 1 CDS steps). Each line corresponds to the GOC profile of the reference against another species. The species are organized from the phylogenetically closest to the furthest compared to S. ambofaciens . The vertical black dotted line represents the location of the dnaA gene delineating the two replichores. “NA” (in yellow) indicates the absence of orthologs in the corresponding window. S. ambofaciens ATCC 23877 also harbors a circular pSAM1 plasmid (≈ 89 kbp). Abbreviations: ‘Actino. Sign. CDSs’ (coding sequences of the actinobacterial signature); BGC (biosynthetic gene cluster); ‘C.’ (congocidine BGC); ‘Chr.’ (whole chromosome); GIs (genes belonging to genomic islands); ‘K.’ (kinamycin BGC); ‘Persist.>0.95 CDSs’ (coding sequences of S. ambofaciens ATCC 23877 presenting a gene persistence superior to 95 % in 124 other Streptomyces genomes); ‘Transl. CDSs’ (genes encoding functions involved in translation process and/or RNA stability); NAPSFs (nucleoid associated proteins and structural factors); SMBGCs (specialized metabolite BGCs); ‘Sp.’ <t>(spiramycin</t> BGC); ‘St.’ (stambomycin BGC); ‘rRNA’ (ribosomal RNA).
    Spiramycin, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    1) Product Images from "Dynamics of the compartmentalized Streptomyces chromosome during metabolic differentiation"

    Article Title: Dynamics of the compartmentalized Streptomyces chromosome during metabolic differentiation

    Journal: bioRxiv

    doi: 10.1101/2020.12.09.415976

    The terminal inverted repeats (TIRs, 200 kb) and the origin of replication ( oriC ) are indicated in black. The distribution of gene presenting features of interest are represented in blue or red when enriched in the central region or within the extremities (defined by the first and last rDNA operons), respectively (see Supplementary Table 3 for a statistical analysis of the data). The dot and asterisk indicate the position of pSAM2 and of a complete prophage, respectively. The position of the SMBGC encoding the biosynthesis of all known antibacterial compounds of S. ambofaciens ATCC 23877 is also indicated. The level of synteny along the chromosome of S. ambofaciens ATCC 23877 is represented as a heat map of GOC (Gene Order Conservation) scores using a sliding window (8 CDSs with 1 CDS steps). Each line corresponds to the GOC profile of the reference against another species. The species are organized from the phylogenetically closest to the furthest compared to S. ambofaciens . The vertical black dotted line represents the location of the dnaA gene delineating the two replichores. “NA” (in yellow) indicates the absence of orthologs in the corresponding window. S. ambofaciens ATCC 23877 also harbors a circular pSAM1 plasmid (≈ 89 kbp). Abbreviations: ‘Actino. Sign. CDSs’ (coding sequences of the actinobacterial signature); BGC (biosynthetic gene cluster); ‘C.’ (congocidine BGC); ‘Chr.’ (whole chromosome); GIs (genes belonging to genomic islands); ‘K.’ (kinamycin BGC); ‘Persist.>0.95 CDSs’ (coding sequences of S. ambofaciens ATCC 23877 presenting a gene persistence superior to 95 % in 124 other Streptomyces genomes); ‘Transl. CDSs’ (genes encoding functions involved in translation process and/or RNA stability); NAPSFs (nucleoid associated proteins and structural factors); SMBGCs (specialized metabolite BGCs); ‘Sp.’ (spiramycin BGC); ‘St.’ (stambomycin BGC); ‘rRNA’ (ribosomal RNA).
    Figure Legend Snippet: The terminal inverted repeats (TIRs, 200 kb) and the origin of replication ( oriC ) are indicated in black. The distribution of gene presenting features of interest are represented in blue or red when enriched in the central region or within the extremities (defined by the first and last rDNA operons), respectively (see Supplementary Table 3 for a statistical analysis of the data). The dot and asterisk indicate the position of pSAM2 and of a complete prophage, respectively. The position of the SMBGC encoding the biosynthesis of all known antibacterial compounds of S. ambofaciens ATCC 23877 is also indicated. The level of synteny along the chromosome of S. ambofaciens ATCC 23877 is represented as a heat map of GOC (Gene Order Conservation) scores using a sliding window (8 CDSs with 1 CDS steps). Each line corresponds to the GOC profile of the reference against another species. The species are organized from the phylogenetically closest to the furthest compared to S. ambofaciens . The vertical black dotted line represents the location of the dnaA gene delineating the two replichores. “NA” (in yellow) indicates the absence of orthologs in the corresponding window. S. ambofaciens ATCC 23877 also harbors a circular pSAM1 plasmid (≈ 89 kbp). Abbreviations: ‘Actino. Sign. CDSs’ (coding sequences of the actinobacterial signature); BGC (biosynthetic gene cluster); ‘C.’ (congocidine BGC); ‘Chr.’ (whole chromosome); GIs (genes belonging to genomic islands); ‘K.’ (kinamycin BGC); ‘Persist.>0.95 CDSs’ (coding sequences of S. ambofaciens ATCC 23877 presenting a gene persistence superior to 95 % in 124 other Streptomyces genomes); ‘Transl. CDSs’ (genes encoding functions involved in translation process and/or RNA stability); NAPSFs (nucleoid associated proteins and structural factors); SMBGCs (specialized metabolite BGCs); ‘Sp.’ (spiramycin BGC); ‘St.’ (stambomycin BGC); ‘rRNA’ (ribosomal RNA).

    Techniques Used: Plasmid Preparation

    3C-seq and RNA-seq were performed on S. ambofaciens grown in exponential phase in YEME medium (24 h, C6 condition). The normalized contact map was obtained from asynchronous populations. The x and y axes represent the genomic coordinates of the reference genome. To simplify the analyses, TIRs were removed from the reference genome. In panel A , the colour scale reflects the frequency of the contacts between genome loci, from white (rare contacts) to dark purple (frequent contacts). In panel B , the frontier index analysis is able to detect, for a given genome 10 kb bin, the change in the contact bias with their neighbouring bins. Thus, a boundary is defined as any bin in which there is a change in the right bias of contacts towards the left bias (± 2 bin, green and orange peaks, respectively). Red and black circles indicate the position of rDNAs and highly expressed genes (HEGs), respectively. In panel C , the normalized counts (DESeq2 analysis) measured in cells grown in the same condition were mapped on S. ambofaciens chromosome and binned in 10 kb. The panel D highlights genomic and transcriptional features of interest. The right and left compartment were defined owing the outer boundaries of the central compartment, which correspond to the first and last rDNA operon position. The ‘synteny-break’ right arm corresponds to the beginning of the spiramycin BGC. The replicate of this experiment is presented in the Supplementary Fig. 3.A .
    Figure Legend Snippet: 3C-seq and RNA-seq were performed on S. ambofaciens grown in exponential phase in YEME medium (24 h, C6 condition). The normalized contact map was obtained from asynchronous populations. The x and y axes represent the genomic coordinates of the reference genome. To simplify the analyses, TIRs were removed from the reference genome. In panel A , the colour scale reflects the frequency of the contacts between genome loci, from white (rare contacts) to dark purple (frequent contacts). In panel B , the frontier index analysis is able to detect, for a given genome 10 kb bin, the change in the contact bias with their neighbouring bins. Thus, a boundary is defined as any bin in which there is a change in the right bias of contacts towards the left bias (± 2 bin, green and orange peaks, respectively). Red and black circles indicate the position of rDNAs and highly expressed genes (HEGs), respectively. In panel C , the normalized counts (DESeq2 analysis) measured in cells grown in the same condition were mapped on S. ambofaciens chromosome and binned in 10 kb. The panel D highlights genomic and transcriptional features of interest. The right and left compartment were defined owing the outer boundaries of the central compartment, which correspond to the first and last rDNA operon position. The ‘synteny-break’ right arm corresponds to the beginning of the spiramycin BGC. The replicate of this experiment is presented in the Supplementary Fig. 3.A .

    Techniques Used: 3C-Seq, RNA Sequencing Assay

    spiramycin  (ATCC)


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    ATCC spiramycin
    Spiramycin, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spiramycin/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    spiramycin - by Bioz Stars, 2023-06
    86/100 stars

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    spiramycin  (ATCC)


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    ATCC spiramycin
    Spiramycin, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spiramycin/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    spiramycin - by Bioz Stars, 2023-06
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