Journal: Turkish Archives of Otorhinolaryngology

Article Title: Antimicrobial Activity of Garlic Derivatives on Common Causative Microorganisms of the External Ear Canal and Chronic Middle Ear Infections

doi: 10.5152/tao.2019.4413

Figure Lengend Snippet: Antifungal activity (MIC and MFC) of the allicin, S-allylcysteine, S-allylmercaptocysteine, and diallyl disulfide

Article Snippet: The MICs of SAMC were > 640 μg/mL for the tested bacteria and yeasts. table ft1 table-wrap mode="anchored" t5 caption a7 S-Allylcysteine Allicin S-Allylmercaptocysteine Diallyl disulfide Control antibiotic (Ciprofloxacin) MIC MBC MIC MBC MIC MBC MIC MBC MIC E. coli ATCC 25922 0.125 0.250 0.125 0.250 >640 >640 80 160 0.125 K. pneumoniae ATCC 700603 20 40 20 40 >640 >640 40 80 0.250 P. aeruginosa ATCC 27853 80 160 80 160 >640 >640 160 320 1 A. baumannii ATCC 19606 20 40 20 40 >640 >640 40 80 0.03 S. aureus ATCC 29213 10 20 10 20 >640 >640 80 160 0.06 E. faecium ATCC BAA2127 5 20 5 20 >640 >640 40 160 0.06 Open in a separate window MIC: minimum inhibitory concentrations (μg/mL); MBC: minimum bactericidal concentration (μg/mL) Antibacterial activity (MIC and MBC) of the allicin, S-allylcysteine, S-allylmercaptocysteine, and diallyl disulfide table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 S-Allylcysteine Allicin S-Allylmercaptocysteine Diallyl disulfide Control antifungal (fluconazole) MIC MFC MIC MFC MIC MFC MIC MFC MIC C. albicans ATCC 102316 80 160 80 160 >640 >640 20 40 0.5 C. tropicalis ATCC 13803 40 80 40 80 >640 >640 40 80 0.5 Open in a separate window MIC: minimum inhibitory concentrations (μg/mL); MFC: minimum fungicidal concentration (μg/mL) Antifungal activity (MIC and MFC) of the allicin, S-allylcysteine, S-allylmercaptocysteine, and diallyl disulfide No antimicrobial activities were detected at the tested concentrations of methanol, HCL and DMSO.

Techniques: Activity Assay

Journal: eLife

Article Title: PAX3-FOXO1 transgenic zebrafish models identify HES3 as a mediator of rhabdomyosarcoma tumorigenesis

doi: 10.7554/eLife.33800

Figure Lengend Snippet: Shown are the survival curves for the following promoters and the total number of zebrafish embryos analyzed for each condition: beta actin ( n = 120), mitfa ( n = 45), fli1 ( n = 81), ubi ( n = 160), unc503 ( n = 108), cmv ( n = 20), SA-splice acceptor ( n = 153).

Article Snippet: The ubi promoter was a kind gift from Len Zon (Addgene #27320) , the unc503 promoter from Peter Currie (Addgene #64020) , the fli1 promoter and 3’ entry 2A-mCherry from Nathan Lawson (Addgene #31160 and #26031) ( ; ), and the mitfa promoter from James Lister (Addgene #81234).

Techniques:

Journal: eLife

Article Title: PAX3-FOXO1 transgenic zebrafish models identify HES3 as a mediator of rhabdomyosarcoma tumorigenesis

doi: 10.7554/eLife.33800

Figure Lengend Snippet:

Article Snippet: The ubi promoter was a kind gift from Len Zon (Addgene #27320) , the unc503 promoter from Peter Currie (Addgene #64020) , the fli1 promoter and 3’ entry 2A-mCherry from Nathan Lawson (Addgene #31160 and #26031) ( ; ), and the mitfa promoter from James Lister (Addgene #81234).

Techniques: Mutagenesis, Injection, Transfection, Construct, Western Blot, Staining, Recombinant, Plasmid Preparation, Generated, Clone Assay, Sequencing, In Situ, Stripping Membranes, Software

Journal: eLife

Article Title: PAX3-FOXO1 transgenic zebrafish models identify HES3 as a mediator of rhabdomyosarcoma tumorigenesis

doi: 10.7554/eLife.33800

Figure Lengend Snippet: Shown are the survival curves for the following promoters and the total number of zebrafish embryos analyzed for each condition: beta actin ( n = 120), mitfa ( n = 45), fli1 ( n = 81), ubi ( n = 160), unc503 ( n = 108), cmv ( n = 20), SA-splice acceptor ( n = 153).

Article Snippet: Recombinant DNA reagent , p5E unc503 , , , PMID:23444339; available from Addgene , , Addgene:64020 , , .

Techniques:

Journal: eLife

Article Title: PAX3-FOXO1 transgenic zebrafish models identify HES3 as a mediator of rhabdomyosarcoma tumorigenesis

doi: 10.7554/eLife.33800

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , p5E unc503 , , , PMID:23444339; available from Addgene , , Addgene:64020 , , .

Techniques: Mutagenesis, Injection, Transfection, Construct, Western Blot, Staining, Recombinant, Plasmid Preparation, Generated, Clone Assay, Sequencing, In Situ, Stripping Membranes, Software