paecilomyces variotii cbs 62866  (ATCC)

Journal: PLOS ONE

Article Title: Beneficial modulation of the gut microbiome by leachates of Penicillium purpurogenum in the presence of clays: A model for the preparation and efficacy of historical Lemnian Earth

doi: 10.1371/journal.pone.0313090

Figure Lengend Snippet: Penicillium purpurogenum was co-cultured with smectite or kaolin clays (2); co-cultures were filtered (3) and their leachates were tested for antibacterial activity in vitro (4) to establish reduction in Gram-positive and Gram-negative bacterial numbers (4a); this step was followed by targeted analysis of fungal metabolites present in each sample (5). The leachates of both co-cultures were then fed to mice, as a supplement to normal chow (6) during in vivo experiments ; mouse stool was removed at day 0 and day 14. The effect of the leachates on the mouse microbiome was investigated via bacterial DNA sequencing (6a) and metabolomic analysis (6b).

Article Snippet: The reference strain DSM 62866 of Talaromyces purpurogenum (formerly referred to as Penicillium purpurogenum – Pp , from the Leibniz Institute DSMZ· German Collection of Microorganisms and Cell Cultures) was used for microbiological analysis and the extraction of metabolites.

Techniques: Cell Culture, Activity Assay, In Vitro, In Vivo, DNA Sequencing