Journal: Scientific Reports

Article Title: Dynamic and functional changes in vascular endothelial cell monolayers under combined hypoxic and inflammatory stimuli

doi: 10.1038/s41598-025-31172-1

Figure Lengend Snippet: Expression of ICAM-1 and VCAM-1 in HUVECs after 6 h of exposure to hypoxia and LPS with/without preconditioning (PreC) by LPS. ( a ) Representative images of maximum intensity projections of confocal microscopy images of HUVECs to the xy -plane. ICAM-1 and VCAM-1 were stained with red and green, respectively. The fluorescence intensity of ICAM-1 integrated over the whole image and normalized by that under the control condition (normoxia and no LPS stimulus), I * ICAM-1 , under ( b ) acute or ( c ) long inflammatory stimulus with LPS and different oxygen conditions. The fluorescence intensity of VCAM-1 integrated over the whole image and normalized by that under the control condition, I * VCAM-1 , under ( d ) acute or ( e ) long inflammatory stimulus with LPS and different oxygen conditions. Error bars show the standard deviation. Significant differences in expression of ICAM-1 and VCAM-1 between the oxygen and LPS conditions were assessed by two-way ANOVA followed by post hoc Tukey’s test for multiple comparisons. † P < 0.1; * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: ICAM-1, VCAM-1, VE-cadherin, HIF-1α, and NF-κB were labeled for 1 h with each antibody diluted in PBS at appropriate concentrations: ICAM-1 antibody (62133, Cell Signaling, USA) at 1:200; VCAM-1 antibody (39036, Cell Signaling) at 1:200; VE-cadherin antibody (sc-9989, Santa Cruz Biotechnology, USA) at 1:200; HIF-1α antibody (ab51608, Abcam, USA) at 1:100; and NF-κB antibody (8242, Cell Signaling) at 1:200.

Techniques: Expressing, Confocal Microscopy, Staining, Fluorescence, Control, Standard Deviation