Journal: Cells

Article Title: E3 Ubiquitin Ligase CHIP Inhibits Haemocyte Proliferation and Differentiation via the Ubiquitination of Runx in the Pacific Oyster

doi: 10.3390/cells13181535

Figure Lengend Snippet: Cg CHIP inhibits agranulocyte differentiation. ( A ) Schematic of the induced differentiation in cultured agranulocytes. ( B ) Representative flow cytometry dot-plots show the gated semi-granulocyte and granulocyte populations differentiated from agranulocytes using the agranulocyte differentiation protocol. ( n = 3). ( C ) The bar graph shows the percentage of differentiated agranulocytes ( n = 3). * p < 0.05, ** p < 0.01, determined by a two-tailed Student’s t -test. ( D , E ) Protein expression levels of the proliferative marker Cg PCNA, immature agranulocyte marker Cg Integrin α4, and mature granulocyte marker Cg AATase, in agranulocytes. β-Tubulin was used as an internal control. Error bars show mean ± standard deviation ( n = 3). * p < 0.05, ** p < 0.01, determined by a two-tailed Student’s t -test.

Article Snippet: The other antibodies used for Western blotting were monoclonal antibodies, including rabbit monoclonal antibodies against PCNA (1:1000, Cell Signaling Technology, Boston, MA, USA), rabbit monoclonal antibodies against Cyclin B1 (1:1000, ABclonal, Wuhan, China), rabbit monoclonal antibodies against CDK2 (1:1000, Cell Signaling Technology, Boston, MA, USA), mouse monoclonal antibodies against Histone H3 (1:5000, Proteintech, Chicago, IL, USA), and HRP-conjugated rabbit monoclonal against beta-Tubulin (1:10,000, Proteintech, Chicago, IL, USA).

Techniques: Cell Culture, Flow Cytometry, Two Tailed Test, Expressing, Marker, Control, Standard Deviation

Journal: Journal of Cancer

Article Title: Exploring the HIF-1α signalling pathway and the mechanism of YiQiHuoXue decoction against Precancerous Lesions of Gastric Cancer based on Network Pharmacology and Molecular Docking

doi: 10.7150/jca.95938

Figure Lengend Snippet: Experiment reagents

Article Snippet: PCNA rabbit monoclonal antibody , CST USA, Inc. , Lot: 0004.

Techniques:

Journal: Journal of Cancer

Article Title: Exploring the HIF-1α signalling pathway and the mechanism of YiQiHuoXue decoction against Precancerous Lesions of Gastric Cancer based on Network Pharmacology and Molecular Docking

doi: 10.7150/jca.95938

Figure Lengend Snippet: The experimental results of YQHXD in the treatment of PLGC rats. (A) HE stained pathological section of gastric tissue (HE *100). (B) Expression of PCNA in gastric mucosa of YQHXD (IHC *400). (C) mRNA expression of key genes of HIF-1α signaling pathway in gastric mucosa of rats. (D) Protein expression of key genes of HIF-1α signaling pathway in gastric mucosa of rats. ( ±s, n=8; Compared with normal group * P <0.05, ** P <0.01, compared with model group Δ P <0.05, ΔΔ P <0.01).

Article Snippet: PCNA rabbit monoclonal antibody , CST USA, Inc. , Lot: 0004.

Techniques: Staining, Expressing