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    CLS Cell Lines Service GmbH vero cells
    Vero Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CLS Cell Lines Service GmbH vero cells
    Vero Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    CLS Cell Lines Service GmbH vero cells
    Vero Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    vero cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH vero cells

    Vero Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The pre-exposure SARS-CoV-2-specific T cell repertoire determines the quality of the immune response to vaccination"

    Article Title: The pre-exposure SARS-CoV-2-specific T cell repertoire determines the quality of the immune response to vaccination

    Journal: Immunity

    doi: 10.1016/j.immuni.2022.08.003


    Figure Legend Snippet:

    Techniques Used: Functional Assay, Recombinant, Staining, Enzyme-linked Immunosorbent Assay, Software

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    CLS Cell Lines Service GmbH vero cells

    Vero Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The pre-exposure SARS-CoV-2-specific T cell repertoire determines the quality of the immune response to vaccination"

    Article Title: The pre-exposure SARS-CoV-2-specific T cell repertoire determines the quality of the immune response to vaccination

    Journal: Immunity

    doi: 10.1016/j.immuni.2022.08.003


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    Techniques Used: Functional Assay, Recombinant, Staining, Enzyme-linked Immunosorbent Assay, Software

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    CLS Cell Lines Service GmbH vero cells

    Vero Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "The pre-exposure SARS-CoV-2-specific T cell repertoire determines the quality of the immune response to vaccination"

    Article Title: The pre-exposure SARS-CoV-2-specific T cell repertoire determines the quality of the immune response to vaccination

    Journal: Immunity

    doi: 10.1016/j.immuni.2022.08.003


    Figure Legend Snippet:

    Techniques Used: Functional Assay, Recombinant, Staining, Enzyme-linked Immunosorbent Assay, Software

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    CLS Cell Lines Service GmbH vero cells
    a. Schematic overview. HEK NM-HA agg donor cells were transfected with combinations of for amphotropic MLV env 10A1 vector, gag/pol plasmid, retroviral transfer vector and/or empty vector. Donors were subsequently cocultured with recipient <t>Vero</t> NM-GFP sol cells. b. Western blot analysis of cell lysates from recipient cells expressing Pit-2. c. Percentage of recipient <t>Vero</t> <t>cells</t> with induced NM-GFP agg cocultured with donors transfected with single plasmids. d. Percentage of recipient cells with induced NM-GFP agg cocultured with donors transfected with combinations of plasmids coding for env , gag/pol or non-viral empty plasmid. e. Percentage of recipient cells with induced NM- GFP agg cocultured with donors that were additionally transfected with/without transfer vector (TV) for virus production. f. Donor cells transfected with/ without mCherry-coding retroviral transfer vector and plasmids coding for gag/pol and env produce virus that is infectious to wildtype cells. g. Representative image of HEK Tau-GFP AD donor cell population. h. HEK Tau-GFP AD donor cells were transfected with combinations of plasmids coding for amphotropic MLV env 10A1, gag/pol , retroviral transfer vector and/or non-viral empty vector. Donors were subsequently cocultured with recipient Vero Tau- FR sol cells. Alternatively, donor EVs were added to recipient cells. i. Confocal image of HEK Tau-GFP AD donor cells. i. Percentage of recipient cells with induced Tau-FR agg upon coculture with donors transfected with individual plasmids. j. Percentage of recipient cells with induced Tau-FR agg upon coculture with donors transfected with plasmid combinations. k. Percentage of recipient cells with induced Tau-FR agg upon coculture with donors transfected with plasmid combinations and transfer vector. l. Transfection of plasmids coding for gag/pol and env simultaneously increases particle secretion. m. Percentage of recipient cells with Tau-FR agg exposed to conditioned medium of donors adjusted for comparable particle numbers. All data are shown as the means ± SD from two (m), three (l) or six (c-e, i-k) replicate cell cultures. Three (c-e, i-m) independent experiments were carried out with similar results. P-values calculated by two-tailed unpaired Student’s t-test (e, k-m) or one-way ANOVA. (c, d, i, j, l). Source data are provided as a Source Data file.
    Vero Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Endogenous retroviruses promote prion-like spreading of proteopathic seeds"

    Article Title: Endogenous retroviruses promote prion-like spreading of proteopathic seeds

    Journal: bioRxiv

    doi: 10.1101/2022.05.06.490866

    a. Schematic overview. HEK NM-HA agg donor cells were transfected with combinations of for amphotropic MLV env 10A1 vector, gag/pol plasmid, retroviral transfer vector and/or empty vector. Donors were subsequently cocultured with recipient Vero NM-GFP sol cells. b. Western blot analysis of cell lysates from recipient cells expressing Pit-2. c. Percentage of recipient Vero cells with induced NM-GFP agg cocultured with donors transfected with single plasmids. d. Percentage of recipient cells with induced NM-GFP agg cocultured with donors transfected with combinations of plasmids coding for env , gag/pol or non-viral empty plasmid. e. Percentage of recipient cells with induced NM- GFP agg cocultured with donors that were additionally transfected with/without transfer vector (TV) for virus production. f. Donor cells transfected with/ without mCherry-coding retroviral transfer vector and plasmids coding for gag/pol and env produce virus that is infectious to wildtype cells. g. Representative image of HEK Tau-GFP AD donor cell population. h. HEK Tau-GFP AD donor cells were transfected with combinations of plasmids coding for amphotropic MLV env 10A1, gag/pol , retroviral transfer vector and/or non-viral empty vector. Donors were subsequently cocultured with recipient Vero Tau- FR sol cells. Alternatively, donor EVs were added to recipient cells. i. Confocal image of HEK Tau-GFP AD donor cells. i. Percentage of recipient cells with induced Tau-FR agg upon coculture with donors transfected with individual plasmids. j. Percentage of recipient cells with induced Tau-FR agg upon coculture with donors transfected with plasmid combinations. k. Percentage of recipient cells with induced Tau-FR agg upon coculture with donors transfected with plasmid combinations and transfer vector. l. Transfection of plasmids coding for gag/pol and env simultaneously increases particle secretion. m. Percentage of recipient cells with Tau-FR agg exposed to conditioned medium of donors adjusted for comparable particle numbers. All data are shown as the means ± SD from two (m), three (l) or six (c-e, i-k) replicate cell cultures. Three (c-e, i-m) independent experiments were carried out with similar results. P-values calculated by two-tailed unpaired Student’s t-test (e, k-m) or one-way ANOVA. (c, d, i, j, l). Source data are provided as a Source Data file.
    Figure Legend Snippet: a. Schematic overview. HEK NM-HA agg donor cells were transfected with combinations of for amphotropic MLV env 10A1 vector, gag/pol plasmid, retroviral transfer vector and/or empty vector. Donors were subsequently cocultured with recipient Vero NM-GFP sol cells. b. Western blot analysis of cell lysates from recipient cells expressing Pit-2. c. Percentage of recipient Vero cells with induced NM-GFP agg cocultured with donors transfected with single plasmids. d. Percentage of recipient cells with induced NM-GFP agg cocultured with donors transfected with combinations of plasmids coding for env , gag/pol or non-viral empty plasmid. e. Percentage of recipient cells with induced NM- GFP agg cocultured with donors that were additionally transfected with/without transfer vector (TV) for virus production. f. Donor cells transfected with/ without mCherry-coding retroviral transfer vector and plasmids coding for gag/pol and env produce virus that is infectious to wildtype cells. g. Representative image of HEK Tau-GFP AD donor cell population. h. HEK Tau-GFP AD donor cells were transfected with combinations of plasmids coding for amphotropic MLV env 10A1, gag/pol , retroviral transfer vector and/or non-viral empty vector. Donors were subsequently cocultured with recipient Vero Tau- FR sol cells. Alternatively, donor EVs were added to recipient cells. i. Confocal image of HEK Tau-GFP AD donor cells. i. Percentage of recipient cells with induced Tau-FR agg upon coculture with donors transfected with individual plasmids. j. Percentage of recipient cells with induced Tau-FR agg upon coculture with donors transfected with plasmid combinations. k. Percentage of recipient cells with induced Tau-FR agg upon coculture with donors transfected with plasmid combinations and transfer vector. l. Transfection of plasmids coding for gag/pol and env simultaneously increases particle secretion. m. Percentage of recipient cells with Tau-FR agg exposed to conditioned medium of donors adjusted for comparable particle numbers. All data are shown as the means ± SD from two (m), three (l) or six (c-e, i-k) replicate cell cultures. Three (c-e, i-m) independent experiments were carried out with similar results. P-values calculated by two-tailed unpaired Student’s t-test (e, k-m) or one-way ANOVA. (c, d, i, j, l). Source data are provided as a Source Data file.

    Techniques Used: Transfection, Plasmid Preparation, Western Blot, Expressing, Two Tailed Test

    a. Experimental workflow. Donor HEK cells stably propagating aggregated Tau-GFP AD were transfected with plasmid coding for V5 epitope-tagged Syncytin-1 (Syn- V5) and were subsequently cocultured with recipient HEK or Vero cells expressing Tau- FR sol . b. Western blot analysis of donor clone transfected with plasmids coding for Syn- V5. c. Coculture of donor and HEK recipient cells. Note that we have not stained the donors in this experiment. d. Quantitative analysis of the percentage of recipient cells with induced aggregates upon coculture. e. Coculture of donor and Vero recipient cells. f. Quantitative analysis of the percentage of recipient cells with induced aggregates. All data are shown as the means ± SD from 6 (d, f) replicate cell cultures. Three (d, f) independent experiments were carried out with similar results. P-values calculated by two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.
    Figure Legend Snippet: a. Experimental workflow. Donor HEK cells stably propagating aggregated Tau-GFP AD were transfected with plasmid coding for V5 epitope-tagged Syncytin-1 (Syn- V5) and were subsequently cocultured with recipient HEK or Vero cells expressing Tau- FR sol . b. Western blot analysis of donor clone transfected with plasmids coding for Syn- V5. c. Coculture of donor and HEK recipient cells. Note that we have not stained the donors in this experiment. d. Quantitative analysis of the percentage of recipient cells with induced aggregates upon coculture. e. Coculture of donor and Vero recipient cells. f. Quantitative analysis of the percentage of recipient cells with induced aggregates. All data are shown as the means ± SD from 6 (d, f) replicate cell cultures. Three (d, f) independent experiments were carried out with similar results. P-values calculated by two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.

    Techniques Used: Stable Transfection, Transfection, Plasmid Preparation, Expressing, Western Blot, Staining, Two Tailed Test

    vero cells  (CLS Cell Lines Service GmbH)


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    Development of SARS-CoV-2 neutralising antibodies (VNA) after first (empty circles) and second (filled circles) immunisation with the vector vaccine AZD1222 or the messenger ribonucleic acid (mRNA)-based vaccines BNT162b2 or mRNA-1273. A surrogate neutralisation assay ( A ) and a <t>Vero-cell-based</t> virus-neutralisation test (cVNT) using the SARS-CoV-2 variant of concern B.1.1.7 (alpha) strain ( B ) were applied to measure the VNAs. The assay cut-offs are indicated by dashed lines. The median and the 95% confidence interval were calculated for each group in A and B . Ns non-significant; * p < 0.05; *** p < 0.001; **** p < 0.0001 (Kruskal-Wallis test)
    Vero Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Humoral immune response after different SARS-CoV-2 vaccination regimens"

    Article Title: Humoral immune response after different SARS-CoV-2 vaccination regimens

    Journal: BMC Medicine

    doi: 10.1186/s12916-021-02231-x

    Development of SARS-CoV-2 neutralising antibodies (VNA) after first (empty circles) and second (filled circles) immunisation with the vector vaccine AZD1222 or the messenger ribonucleic acid (mRNA)-based vaccines BNT162b2 or mRNA-1273. A surrogate neutralisation assay ( A ) and a Vero-cell-based virus-neutralisation test (cVNT) using the SARS-CoV-2 variant of concern B.1.1.7 (alpha) strain ( B ) were applied to measure the VNAs. The assay cut-offs are indicated by dashed lines. The median and the 95% confidence interval were calculated for each group in A and B . Ns non-significant; * p < 0.05; *** p < 0.001; **** p < 0.0001 (Kruskal-Wallis test)
    Figure Legend Snippet: Development of SARS-CoV-2 neutralising antibodies (VNA) after first (empty circles) and second (filled circles) immunisation with the vector vaccine AZD1222 or the messenger ribonucleic acid (mRNA)-based vaccines BNT162b2 or mRNA-1273. A surrogate neutralisation assay ( A ) and a Vero-cell-based virus-neutralisation test (cVNT) using the SARS-CoV-2 variant of concern B.1.1.7 (alpha) strain ( B ) were applied to measure the VNAs. The assay cut-offs are indicated by dashed lines. The median and the 95% confidence interval were calculated for each group in A and B . Ns non-significant; * p < 0.05; *** p < 0.001; **** p < 0.0001 (Kruskal-Wallis test)

    Techniques Used: Plasmid Preparation, Variant Assay

    Anti-SARS-CoV-2 immunoglobulin G (IgG) response in Binding Antibody Units (BAU) per millilitre (ml) after first (open circles) and second (filled circles) immunisation with the vector vaccine AZD1222 (green), the messenger ribonucleic acid (mRNA)-based vaccine BNT162b2 (blue) and after a heterologous vaccination scheme, starting with AZD1222, followed by an mRNA-based vaccine boost (BNT162b2 or mRNA-1273; red) with regard to the detection of virus-neutralising antibodies (VNA). The latter were measured in a Vero-cell-based neutralisation test (cVNT) using the SARS-CoV-2 variant of concern B.1.1.7 (alpha). Cut-off values for positivity of the anti-trimeric spike (S) IgG assay ( A ), anti-S IgG assay ( B ) and anti-receptor binding domain (RBD) IgG assay ( C ), respectively, and the cVNT cut-off value for the presence of VNA are indicated by black dashed lines. The Spearman correlation coefficients of log(reciprocal titre) were calculated with 0.86, 0.86 and 0.88, respectively. The probability of detecting VNA at a given BAU/ml in the anti-SARS-CoV-2 IgG assays was calculated by logistic regression ( D – F ): VNA were present in 95% of samples when IgG concentrations of 886 BAU/ml (anti-trimeric S IgG), 323 BAU/ml (anti-S IgG) and 448 BAU/ml (anti-RBD IgG), respectively, were measured (green dashed lines; 95% confidence intervals (CI) 59.4 to 99.6%). Vertical black dashed lines represent the threshold values set by the manufacturers of the antibody assay; red dashed lines represent the BAU/ml concentrations (anti-trimeric S IgG: 350 BAU/ml; anti-S IgG: 119 BAU/ml; anti-RBD IgG: 174 BAU/ml) with a 50% probability of VNA detection. The distribution of the cVNT titres, the medians, and the 95% CIs between the three plotted thresholds (dashed black, red and green lines in A – F ) are shown ( G – I )
    Figure Legend Snippet: Anti-SARS-CoV-2 immunoglobulin G (IgG) response in Binding Antibody Units (BAU) per millilitre (ml) after first (open circles) and second (filled circles) immunisation with the vector vaccine AZD1222 (green), the messenger ribonucleic acid (mRNA)-based vaccine BNT162b2 (blue) and after a heterologous vaccination scheme, starting with AZD1222, followed by an mRNA-based vaccine boost (BNT162b2 or mRNA-1273; red) with regard to the detection of virus-neutralising antibodies (VNA). The latter were measured in a Vero-cell-based neutralisation test (cVNT) using the SARS-CoV-2 variant of concern B.1.1.7 (alpha). Cut-off values for positivity of the anti-trimeric spike (S) IgG assay ( A ), anti-S IgG assay ( B ) and anti-receptor binding domain (RBD) IgG assay ( C ), respectively, and the cVNT cut-off value for the presence of VNA are indicated by black dashed lines. The Spearman correlation coefficients of log(reciprocal titre) were calculated with 0.86, 0.86 and 0.88, respectively. The probability of detecting VNA at a given BAU/ml in the anti-SARS-CoV-2 IgG assays was calculated by logistic regression ( D – F ): VNA were present in 95% of samples when IgG concentrations of 886 BAU/ml (anti-trimeric S IgG), 323 BAU/ml (anti-S IgG) and 448 BAU/ml (anti-RBD IgG), respectively, were measured (green dashed lines; 95% confidence intervals (CI) 59.4 to 99.6%). Vertical black dashed lines represent the threshold values set by the manufacturers of the antibody assay; red dashed lines represent the BAU/ml concentrations (anti-trimeric S IgG: 350 BAU/ml; anti-S IgG: 119 BAU/ml; anti-RBD IgG: 174 BAU/ml) with a 50% probability of VNA detection. The distribution of the cVNT titres, the medians, and the 95% CIs between the three plotted thresholds (dashed black, red and green lines in A – F ) are shown ( G – I )

    Techniques Used: Binding Assay, Plasmid Preparation, Variant Assay

    Correlation of the surrogate neutralisation test (sVNT) results with results obtained by the laboratory-developed Vero-cell-based virus-neutralisation test (cVNT) using a B.1.1.7 strain as antigen ( A ). The Spearman correlation coefficient of log(reciprocal titre) was calculated with 0.88; empty circles : first vaccination; filled circles: second vaccination; red: heterologous vaccination with AZD1222/mRNA; green: homologous vaccination with AZD1222; blue: homologous vaccination with BNT162b2. Probability of detecting virus-neutralising antibodies (VNA) with the cVNT at a given percentage inhibition of sVNT calculated by logistic regression (B) ; e.g. at 20% inhibition (black dashed line), 63% inhibition (red dashed line), and at 87% inhibition of sVNT (green dashed line), the probabilities of detecting VNA with cVNT are 4% (95% confidence interval (CI) 1–16%), 50 % (95% CI 34–66 %) and 85% (95% CI 73–92%), respectively. The distribution of the cVNT titres, their medians, and their 95% CIs between the three plotted thresholds (dashed black, red and green lines in A , B ) are shown ( C )
    Figure Legend Snippet: Correlation of the surrogate neutralisation test (sVNT) results with results obtained by the laboratory-developed Vero-cell-based virus-neutralisation test (cVNT) using a B.1.1.7 strain as antigen ( A ). The Spearman correlation coefficient of log(reciprocal titre) was calculated with 0.88; empty circles : first vaccination; filled circles: second vaccination; red: heterologous vaccination with AZD1222/mRNA; green: homologous vaccination with AZD1222; blue: homologous vaccination with BNT162b2. Probability of detecting virus-neutralising antibodies (VNA) with the cVNT at a given percentage inhibition of sVNT calculated by logistic regression (B) ; e.g. at 20% inhibition (black dashed line), 63% inhibition (red dashed line), and at 87% inhibition of sVNT (green dashed line), the probabilities of detecting VNA with cVNT are 4% (95% confidence interval (CI) 1–16%), 50 % (95% CI 34–66 %) and 85% (95% CI 73–92%), respectively. The distribution of the cVNT titres, their medians, and their 95% CIs between the three plotted thresholds (dashed black, red and green lines in A , B ) are shown ( C )

    Techniques Used: Inhibition

    Presence of virus-neutralising antibodies (VNA) against the SARS-CoV-2 variants of concern B.1.1.7 (alpha, filled circles) and B.1.617.2 (delta, empty circles) after the second immunisation. Sera from 26 age- and gender-matched individuals who received a heterologous (AZD1222/BNT162b2, n = 9) or a homologous vaccination scheme (AZD1222/AZD1222, n = 9; BNT162b2/BNT162b2, n = 8) were tested (see Table ). An individual VNA titre > 1:10 was defined as neutralising in our Vero-cell-based virus-neutralisation test (cVNT). †The significance of the median VNA titre differences against B.1.1.7 and B.1.617.2 was calculated using the Wilcoxon test (** p < 0.01). ‡Comparison of the median VNA titre differences achieved with different immunisation schemes against B.1.617.2 (Kruskal-Wallis test; ns not significant; ** p < 0.01; **** p < 0.0001)
    Figure Legend Snippet: Presence of virus-neutralising antibodies (VNA) against the SARS-CoV-2 variants of concern B.1.1.7 (alpha, filled circles) and B.1.617.2 (delta, empty circles) after the second immunisation. Sera from 26 age- and gender-matched individuals who received a heterologous (AZD1222/BNT162b2, n = 9) or a homologous vaccination scheme (AZD1222/AZD1222, n = 9; BNT162b2/BNT162b2, n = 8) were tested (see Table ). An individual VNA titre > 1:10 was defined as neutralising in our Vero-cell-based virus-neutralisation test (cVNT). †The significance of the median VNA titre differences against B.1.1.7 and B.1.617.2 was calculated using the Wilcoxon test (** p < 0.01). ‡Comparison of the median VNA titre differences achieved with different immunisation schemes against B.1.617.2 (Kruskal-Wallis test; ns not significant; ** p < 0.01; **** p < 0.0001)

    Techniques Used:

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    CLS Cell Lines Service GmbH vero cells
    a . HEK NM-HA agg or Tau-GFP agg cells, transfected with plasmid coding for CoV-2 spike S or Mock transfected, were cocultured with respective recipient HEK cells overexpressing/not overexpressing human ACE2. Alternatively, donors were cocultured with <t>Vero</t> <t>cells</t> stably expressing NM-GFP sol or Tau-FR sol . b Expression of CoV-2 spike S in donor HEK cells. c Cocultures of transfected HEK NM-HA agg donors and recipient Vero NM-GFP sol cells. d Cocultures of transfected donor HEK Tau-GFP AD cells with recipient Vero Tau-FR sol cells. e Western blot of recipient HEK cells expressing ACE2-Flag. The blot was probed with anti-ACE2 antibodies. f – h Percentage of recipients with induced aggregates. i Presence of spike S on EV. j EV from donors transfected with empty or spike S plasmid were added to Vero cells. The percentage of recipients with induced aggregates was assessed 2 days later. k Particle numbers. l , m Percentage of EV exposed recipients with induced aggregates All data are shown as the means ± SD from three ( l , m ) or six ( f – h , k ) replicate cell cultures. Two ( f – h , k – m ) independent experiments were carried out with similar results. P values calculated by two-tailed unpaired Student´s t test ( k – m ) or one-way ANOVA ( f – h ). ns nonsignificant. Source data are provided as a Source Data file.
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    1) Product Images from "Highly efficient intercellular spreading of protein misfolding mediated by viral ligand-receptor interactions"

    Article Title: Highly efficient intercellular spreading of protein misfolding mediated by viral ligand-receptor interactions

    Journal: Nature Communications

    doi: 10.1038/s41467-021-25855-2

    a . HEK NM-HA agg or Tau-GFP agg cells, transfected with plasmid coding for CoV-2 spike S or Mock transfected, were cocultured with respective recipient HEK cells overexpressing/not overexpressing human ACE2. Alternatively, donors were cocultured with Vero cells stably expressing NM-GFP sol or Tau-FR sol . b Expression of CoV-2 spike S in donor HEK cells. c Cocultures of transfected HEK NM-HA agg donors and recipient Vero NM-GFP sol cells. d Cocultures of transfected donor HEK Tau-GFP AD cells with recipient Vero Tau-FR sol cells. e Western blot of recipient HEK cells expressing ACE2-Flag. The blot was probed with anti-ACE2 antibodies. f – h Percentage of recipients with induced aggregates. i Presence of spike S on EV. j EV from donors transfected with empty or spike S plasmid were added to Vero cells. The percentage of recipients with induced aggregates was assessed 2 days later. k Particle numbers. l , m Percentage of EV exposed recipients with induced aggregates All data are shown as the means ± SD from three ( l , m ) or six ( f – h , k ) replicate cell cultures. Two ( f – h , k – m ) independent experiments were carried out with similar results. P values calculated by two-tailed unpaired Student´s t test ( k – m ) or one-way ANOVA ( f – h ). ns nonsignificant. Source data are provided as a Source Data file.
    Figure Legend Snippet: a . HEK NM-HA agg or Tau-GFP agg cells, transfected with plasmid coding for CoV-2 spike S or Mock transfected, were cocultured with respective recipient HEK cells overexpressing/not overexpressing human ACE2. Alternatively, donors were cocultured with Vero cells stably expressing NM-GFP sol or Tau-FR sol . b Expression of CoV-2 spike S in donor HEK cells. c Cocultures of transfected HEK NM-HA agg donors and recipient Vero NM-GFP sol cells. d Cocultures of transfected donor HEK Tau-GFP AD cells with recipient Vero Tau-FR sol cells. e Western blot of recipient HEK cells expressing ACE2-Flag. The blot was probed with anti-ACE2 antibodies. f – h Percentage of recipients with induced aggregates. i Presence of spike S on EV. j EV from donors transfected with empty or spike S plasmid were added to Vero cells. The percentage of recipients with induced aggregates was assessed 2 days later. k Particle numbers. l , m Percentage of EV exposed recipients with induced aggregates All data are shown as the means ± SD from three ( l , m ) or six ( f – h , k ) replicate cell cultures. Two ( f – h , k – m ) independent experiments were carried out with similar results. P values calculated by two-tailed unpaired Student´s t test ( k – m ) or one-way ANOVA ( f – h ). ns nonsignificant. Source data are provided as a Source Data file.

    Techniques Used: Transfection, Plasmid Preparation, Stable Transfection, Expressing, Western Blot, Two Tailed Test

    vero cells  (CLS Cell Lines Service GmbH)


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    CLS Cell Lines Service GmbH vero cells
    Development of virus neutralizing antibodies against a ‘wildtype’ SARS-CoV-2 strain (wt) and against a B.1.1.7 virus after infection with variants of concern (VOCs: n = 10, B.1.1.7, filled green dot; n = 1, B.1.351, empty green dot) or after first (1) and second (2) vaccination with with messenger ribonucleic (BNT162b2; mRNA-1273) and vector (AZD1222) vaccines ( n = 11 age and gender matched vaccinees per vaccine group). Results were obtained in a laboratory-developed <t>Vero-cell</t> based neutralization assay (cVNT) under BSL-3 conditions. A serum dilution of >1:10 is considered as protective. The reciprocal geometric mean titer and the 95% confidence intervals are given. Stars indicate statistical significance (***: p < 0.001) calculated in a paired, non-parametric test (Wilcoxon test).
    Vero Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Development of SARS-CoV-2 Specific IgG and Virus-Neutralizing Antibodies after Infection with Variants of Concern or Vaccination"

    Article Title: Development of SARS-CoV-2 Specific IgG and Virus-Neutralizing Antibodies after Infection with Variants of Concern or Vaccination

    Journal: Vaccines

    doi: 10.3390/vaccines9070700

    Development of virus neutralizing antibodies against a ‘wildtype’ SARS-CoV-2 strain (wt) and against a B.1.1.7 virus after infection with variants of concern (VOCs: n = 10, B.1.1.7, filled green dot; n = 1, B.1.351, empty green dot) or after first (1) and second (2) vaccination with with messenger ribonucleic (BNT162b2; mRNA-1273) and vector (AZD1222) vaccines ( n = 11 age and gender matched vaccinees per vaccine group). Results were obtained in a laboratory-developed Vero-cell based neutralization assay (cVNT) under BSL-3 conditions. A serum dilution of >1:10 is considered as protective. The reciprocal geometric mean titer and the 95% confidence intervals are given. Stars indicate statistical significance (***: p < 0.001) calculated in a paired, non-parametric test (Wilcoxon test).
    Figure Legend Snippet: Development of virus neutralizing antibodies against a ‘wildtype’ SARS-CoV-2 strain (wt) and against a B.1.1.7 virus after infection with variants of concern (VOCs: n = 10, B.1.1.7, filled green dot; n = 1, B.1.351, empty green dot) or after first (1) and second (2) vaccination with with messenger ribonucleic (BNT162b2; mRNA-1273) and vector (AZD1222) vaccines ( n = 11 age and gender matched vaccinees per vaccine group). Results were obtained in a laboratory-developed Vero-cell based neutralization assay (cVNT) under BSL-3 conditions. A serum dilution of >1:10 is considered as protective. The reciprocal geometric mean titer and the 95% confidence intervals are given. Stars indicate statistical significance (***: p < 0.001) calculated in a paired, non-parametric test (Wilcoxon test).

    Techniques Used: Infection, Plasmid Preparation, Neutralization

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    CLS Cell Lines Service GmbH vero cells
    Isolation of SARS-CoV-2 from upper respiratory tract (URT) samples in <t>Vero</t> <t>cells.</t> A successful isolation in cell culture was assumed if the supernatant of the corresponding well had a threshold cycle (Ct) < 20 in the N-gene triplex RT-PCR. The isolation rate was estimated as a function of viral load by mean of the Ct (N-gene triplex RT-PCR) of the original URT sample. The results after the first round on Vero cells and after the third round are shown. Four positive and negative controls (pc, nc) were included. The table below the figure lists the result per URT sample and the corresponding Ct. The cytopathic effect observed after the third round in Vero cells is documented in .
    Vero Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Performance of a Point-of-Care Test for the Rapid Detection of SARS-CoV-2 Antigen"

    Article Title: Performance of a Point-of-Care Test for the Rapid Detection of SARS-CoV-2 Antigen

    Journal: Microorganisms

    doi: 10.3390/microorganisms9010058

    Isolation of SARS-CoV-2 from upper respiratory tract (URT) samples in Vero cells. A successful isolation in cell culture was assumed if the supernatant of the corresponding well had a threshold cycle (Ct) < 20 in the N-gene triplex RT-PCR. The isolation rate was estimated as a function of viral load by mean of the Ct (N-gene triplex RT-PCR) of the original URT sample. The results after the first round on Vero cells and after the third round are shown. Four positive and negative controls (pc, nc) were included. The table below the figure lists the result per URT sample and the corresponding Ct. The cytopathic effect observed after the third round in Vero cells is documented in .
    Figure Legend Snippet: Isolation of SARS-CoV-2 from upper respiratory tract (URT) samples in Vero cells. A successful isolation in cell culture was assumed if the supernatant of the corresponding well had a threshold cycle (Ct) < 20 in the N-gene triplex RT-PCR. The isolation rate was estimated as a function of viral load by mean of the Ct (N-gene triplex RT-PCR) of the original URT sample. The results after the first round on Vero cells and after the third round are shown. Four positive and negative controls (pc, nc) were included. The table below the figure lists the result per URT sample and the corresponding Ct. The cytopathic effect observed after the third round in Vero cells is documented in .

    Techniques Used: Isolation, Cell Culture, Reverse Transcription Polymerase Chain Reaction

    Cytopathic effect after two passages (third round) of 64 upper respiratory tract samples (no. 9–72) in Vero cells with respect to the threshold cycle (Ct) values of the original sample (N-gene triplex RT-PCR). For comparison, positive controls (vc, no. 73–76; own SARS-CoV-2 isolates), negative controls (nc, no. 5–8; SARS-CoV-2 free patient samples) and cell controls (cc, no. 1–4, and ni; cells in medium) are included. Note that the destroyed cell layer in no. 31 represents an artifact and does not correlate to the result of N-gene triplex RT-PCR from the supernatant (compare ).
    Figure Legend Snippet: Cytopathic effect after two passages (third round) of 64 upper respiratory tract samples (no. 9–72) in Vero cells with respect to the threshold cycle (Ct) values of the original sample (N-gene triplex RT-PCR). For comparison, positive controls (vc, no. 73–76; own SARS-CoV-2 isolates), negative controls (nc, no. 5–8; SARS-CoV-2 free patient samples) and cell controls (cc, no. 1–4, and ni; cells in medium) are included. Note that the destroyed cell layer in no. 31 represents an artifact and does not correlate to the result of N-gene triplex RT-PCR from the supernatant (compare ).

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

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