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Image Search Results
Journal: Cell Reports Methods
Article Title: STRAIGHT-IN enables high-throughput targeting of large DNA payloads in human pluripotent stem cells
doi: 10.1016/j.crmeth.2022.100300
Figure Lengend Snippet:
Article Snippet: To target the Bxb1-LP and φC31-LP cassettes to the adeno-associated virus integration site ( AAVS1 ) within intron 1 of PPP1R12C , the cassettes were PCR-amplified with primers containing overlap sequences to clone via NEBuilder® HiFi DNA Assembly into AAVS1_SA_2A_ Neo
Techniques: Virus, Recombinant, Selection, Transfection, Knock-Out, Cloning, Plasmid Preparation, PCR Cloning, cDNA Synthesis, DNA Sequencing, Software, Digital PCR, Imaging, Flow Cytometry