hmgb1  (Chondrex Inc)


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    Chondrex Inc hmgb1
    Hmgb1, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hmgb1/product/Chondrex Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hmgb1 - by Bioz Stars, 2022-08
    94/100 stars

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    Chondrex Inc hmgb1 detection kit
    In vitro immune cell death (ICD) induced by ROS generated from the Fenton-like reaction under the NIR II irradiation. ( a ) Schematic illustration of the DAMPs (CRT, ATP and <t>HMGB1)</t> released from the dying tumor cells to promote the DC mature, triggered by large amount of ROS generated from the degradation of H 2 O 2 by CS-I/J@CM NPs under irradiation. ( b ) CLSM of CRT (red fluorescence) exposed on the cytomembrane (green fluorescence) of GL261 cells, after different treatment with or without CS-I/J@CM NPs (12.5 μg/mL) (scale bar: 20 μm). ( c ) Colocalization of CRT and cytomembrane of GL261 cells. ( d ) Flow cytometry analysis of CRT expose on GL261 cells after co-cultured with or without CS-I/J@CM NPs (12.5 μg/mL). ( e ) Detection of extracellular ATP secreted. ( f ) Detection of extracellular HMGB1 released. ( g ) Flow cytometry analysis of the maturation of DC (CD11c + CD80 + CD86 + ). ( h ) Normalization of the maturation of DC in Fig. 3 g. (*p
    Hmgb1 Detection Kit, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hmgb1 detection kit/product/Chondrex Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hmgb1 detection kit - by Bioz Stars, 2022-08
    94/100 stars
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    In vitro immune cell death (ICD) induced by ROS generated from the Fenton-like reaction under the NIR II irradiation. ( a ) Schematic illustration of the DAMPs (CRT, ATP and HMGB1) released from the dying tumor cells to promote the DC mature, triggered by large amount of ROS generated from the degradation of H 2 O 2 by CS-I/J@CM NPs under irradiation. ( b ) CLSM of CRT (red fluorescence) exposed on the cytomembrane (green fluorescence) of GL261 cells, after different treatment with or without CS-I/J@CM NPs (12.5 μg/mL) (scale bar: 20 μm). ( c ) Colocalization of CRT and cytomembrane of GL261 cells. ( d ) Flow cytometry analysis of CRT expose on GL261 cells after co-cultured with or without CS-I/J@CM NPs (12.5 μg/mL). ( e ) Detection of extracellular ATP secreted. ( f ) Detection of extracellular HMGB1 released. ( g ) Flow cytometry analysis of the maturation of DC (CD11c + CD80 + CD86 + ). ( h ) Normalization of the maturation of DC in Fig. 3 g. (*p

    Journal: Bioactive Materials

    Article Title: Biomimetic nanoparticles directly remodel immunosuppressive microenvironment for boosting glioblastoma immunotherapy

    doi: 10.1016/j.bioactmat.2021.12.029

    Figure Lengend Snippet: In vitro immune cell death (ICD) induced by ROS generated from the Fenton-like reaction under the NIR II irradiation. ( a ) Schematic illustration of the DAMPs (CRT, ATP and HMGB1) released from the dying tumor cells to promote the DC mature, triggered by large amount of ROS generated from the degradation of H 2 O 2 by CS-I/J@CM NPs under irradiation. ( b ) CLSM of CRT (red fluorescence) exposed on the cytomembrane (green fluorescence) of GL261 cells, after different treatment with or without CS-I/J@CM NPs (12.5 μg/mL) (scale bar: 20 μm). ( c ) Colocalization of CRT and cytomembrane of GL261 cells. ( d ) Flow cytometry analysis of CRT expose on GL261 cells after co-cultured with or without CS-I/J@CM NPs (12.5 μg/mL). ( e ) Detection of extracellular ATP secreted. ( f ) Detection of extracellular HMGB1 released. ( g ) Flow cytometry analysis of the maturation of DC (CD11c + CD80 + CD86 + ). ( h ) Normalization of the maturation of DC in Fig. 3 g. (*p

    Article Snippet: The extracellularly released adenine nucleoside triphosphate (ATP) and high-mobility group box 1 (HMGB1) were detected by ATP detection Kit (A22066) (Molecular Probes) and HMGB1 detection Kit (Chondrex), respectively.

    Techniques: In Vitro, Generated, Irradiation, Confocal Laser Scanning Microscopy, Fluorescence, Flow Cytometry, Cell Culture

    Sirt1 inhibits high mobility group box-1 translocation and release. A: Acetylated HMGB1 levels determined by immunoprecipitation. EX527, a Sirt1 inhibitor inhibited sirt1 level and promoted HMGB1 acetylation; B: High mobility group box-1 (HMGB1) expression in the nucleus or cytoplasm determined by Western blot; C: The mRNA and protein levels of Sirt1. BNL CL 2 cells were transfected with control lentiviral vector or Sirt1 shRNA lentiviral vector for 48 h. The mRNA and protein levels of Sirt1 were detected by real-time PCR and Western blot. Multiplicity of infection, refers to the number of lent virus adsorption on the cells and the ratio of the number of cells in culture; D: HMGB1 translocation; E: HMGB1 content in medium determined by ELISA. All data are presented as the mean ± SD. Statistical analysis was done by the Student’s t -test. b P

    Journal: World Journal of Gastroenterology

    Article Title: High mobility group box-1 release from H2O2-injured hepatocytes due to sirt1 functional inhibition

    doi: 10.3748/wjg.v25.i36.5434

    Figure Lengend Snippet: Sirt1 inhibits high mobility group box-1 translocation and release. A: Acetylated HMGB1 levels determined by immunoprecipitation. EX527, a Sirt1 inhibitor inhibited sirt1 level and promoted HMGB1 acetylation; B: High mobility group box-1 (HMGB1) expression in the nucleus or cytoplasm determined by Western blot; C: The mRNA and protein levels of Sirt1. BNL CL 2 cells were transfected with control lentiviral vector or Sirt1 shRNA lentiviral vector for 48 h. The mRNA and protein levels of Sirt1 were detected by real-time PCR and Western blot. Multiplicity of infection, refers to the number of lent virus adsorption on the cells and the ratio of the number of cells in culture; D: HMGB1 translocation; E: HMGB1 content in medium determined by ELISA. All data are presented as the mean ± SD. Statistical analysis was done by the Student’s t -test. b P

    Article Snippet: The levels of HMGB1 in the culture medium were measured by ELISA kit (Chondrex Co. Ltd, Redmond, United States) according to the manufacturer’s instructions.

    Techniques: Translocation Assay, Immunoprecipitation, Expressing, Western Blot, Transfection, Plasmid Preparation, shRNA, Real-time Polymerase Chain Reaction, Infection, Adsorption, Enzyme-linked Immunosorbent Assay

    Cytotoxicity increase and high mobility group box-1 release induced by H 2 O 2 in BNL CL cells. A and B: BNL CL2 cells cultured with H 2 O 2 for 0, 0.5, 2, 8, or 24 h or at different concentrations (0, 100, 500, and 1000 μmol/L); C: 8-hydroxy-2-deoxyguanosine levels in cells treated with 500 μmol/L H 2 O 2 or N-acetylcysteine (NAC); D: High mobility group box-1 (HMGB1) content in medium detected by ELISA; E: Cells were treated with 500 μM H 2 O 2 for different time intervals HMGB1 content in medium detected by ELISA. Cells were pre-treated with NAC for 24 h, and then incubated with or without H 2 O 2 (500 μmol/L) for 8 h. All data are presented as the mean ± SD. Statistical analysis was done by the Student’s t -test. a P

    Journal: World Journal of Gastroenterology

    Article Title: High mobility group box-1 release from H2O2-injured hepatocytes due to sirt1 functional inhibition

    doi: 10.3748/wjg.v25.i36.5434

    Figure Lengend Snippet: Cytotoxicity increase and high mobility group box-1 release induced by H 2 O 2 in BNL CL cells. A and B: BNL CL2 cells cultured with H 2 O 2 for 0, 0.5, 2, 8, or 24 h or at different concentrations (0, 100, 500, and 1000 μmol/L); C: 8-hydroxy-2-deoxyguanosine levels in cells treated with 500 μmol/L H 2 O 2 or N-acetylcysteine (NAC); D: High mobility group box-1 (HMGB1) content in medium detected by ELISA; E: Cells were treated with 500 μM H 2 O 2 for different time intervals HMGB1 content in medium detected by ELISA. Cells were pre-treated with NAC for 24 h, and then incubated with or without H 2 O 2 (500 μmol/L) for 8 h. All data are presented as the mean ± SD. Statistical analysis was done by the Student’s t -test. a P

    Article Snippet: The levels of HMGB1 in the culture medium were measured by ELISA kit (Chondrex Co. Ltd, Redmond, United States) according to the manufacturer’s instructions.

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Incubation

    H 2 O 2 treatment inhibits Sirt1 expression and activity. A and B: BNL CL 2 cells were treated with H 2 O 2 for 0-24 h at 500 μmol/L. Sirt1 levels determined by Western blot; C and D: BNL CL 2 cells were treated with H 2 O 2 at 0-1000 μmol/L for 8 h. Sirt1 levels determined by Western blot; E: Sirt1 deacetylase activity detected by fluorescence spectrophotometry; F: BNL CL 2 cells were treated with H 2 O 2 (500 μmol/L) for 4 h, and then SRT1720, a Sirt1 activator, was added into the medium. The supernatants were collected to determinate high mobility group box-1 content by ELISA. All data are presented as the mean ± SD. Statistical analysis was done by the Student’s t -test. a P

    Journal: World Journal of Gastroenterology

    Article Title: High mobility group box-1 release from H2O2-injured hepatocytes due to sirt1 functional inhibition

    doi: 10.3748/wjg.v25.i36.5434

    Figure Lengend Snippet: H 2 O 2 treatment inhibits Sirt1 expression and activity. A and B: BNL CL 2 cells were treated with H 2 O 2 for 0-24 h at 500 μmol/L. Sirt1 levels determined by Western blot; C and D: BNL CL 2 cells were treated with H 2 O 2 at 0-1000 μmol/L for 8 h. Sirt1 levels determined by Western blot; E: Sirt1 deacetylase activity detected by fluorescence spectrophotometry; F: BNL CL 2 cells were treated with H 2 O 2 (500 μmol/L) for 4 h, and then SRT1720, a Sirt1 activator, was added into the medium. The supernatants were collected to determinate high mobility group box-1 content by ELISA. All data are presented as the mean ± SD. Statistical analysis was done by the Student’s t -test. a P

    Article Snippet: The levels of HMGB1 in the culture medium were measured by ELISA kit (Chondrex Co. Ltd, Redmond, United States) according to the manufacturer’s instructions.

    Techniques: Expressing, Activity Assay, Western Blot, Histone Deacetylase Assay, Fluorescence, Spectrophotometry, Enzyme-linked Immunosorbent Assay

    Sirt1 activity is inhibited by H 2 O 2 through Parp1-NAD + . A and B: NAD + contents in cells treated with H 2 O 2 for different durations or at different concentrations; C: NAD + contents in cells treated with DIQ, a Parp1 inhibitor; D: Sirt1 activity in cells treated with DIQ and NAM, an NAD + inhibitor; E and F: High mobility group box-1 (HMGB1) acetylation in cells treated with DIQ; G: BNL CL2 cells were treated with DIQ or H 2 O 2 . And then HMGB1 contents in medium determined by ELISA; H: HMGB1 contents in medium determined by ELISA in cells treated with CP or Parp1 + transfection; I: Sirt1 activity of cells with Parp1 + transfection. All data are presented as the mean ± SD. Statistical analysis was done by the Student’s t- test. b P

    Journal: World Journal of Gastroenterology

    Article Title: High mobility group box-1 release from H2O2-injured hepatocytes due to sirt1 functional inhibition

    doi: 10.3748/wjg.v25.i36.5434

    Figure Lengend Snippet: Sirt1 activity is inhibited by H 2 O 2 through Parp1-NAD + . A and B: NAD + contents in cells treated with H 2 O 2 for different durations or at different concentrations; C: NAD + contents in cells treated with DIQ, a Parp1 inhibitor; D: Sirt1 activity in cells treated with DIQ and NAM, an NAD + inhibitor; E and F: High mobility group box-1 (HMGB1) acetylation in cells treated with DIQ; G: BNL CL2 cells were treated with DIQ or H 2 O 2 . And then HMGB1 contents in medium determined by ELISA; H: HMGB1 contents in medium determined by ELISA in cells treated with CP or Parp1 + transfection; I: Sirt1 activity of cells with Parp1 + transfection. All data are presented as the mean ± SD. Statistical analysis was done by the Student’s t- test. b P

    Article Snippet: The levels of HMGB1 in the culture medium were measured by ELISA kit (Chondrex Co. Ltd, Redmond, United States) according to the manufacturer’s instructions.

    Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Transfection