Journal: Cell Death & Disease

Article Title: Comparative analysis of S100A10 and S100A11 in MASLD and hepatic cancer development revealed a tumor suppressive role for S100A10

doi: 10.1038/s41419-025-07940-2

Figure Lengend Snippet: A Relative protein level of S100A10 measured by western-blot in the liver of 5 months-old LPTENKO injected at 4 months with AAV8-shCTL ( n = 8), AAV8-shS100A10 ( n = 7), or AAV8-shS100A11 ( n = 7). Tubulin was used as housekeeping protein. Results are expressed as fold change versus the shCTL group. B Relative mRNA level of S100A10 measured by qPCR in the liver of 5 months-old LPTENKO injected at 4 months with AAV8-shCTL ( n = 8), AAV8-shS100A10 ( n = 7), or AAV8-shS100A11 ( n = 7). Cyclophilin A was used as housekeeping gene. Results are expressed as fold-change versus the shCTL group. C Scoring of S100A10 immunohistochemistry performed on liver TMA from HCC patients comparing HCC ( n = 92, two per patient) and adjacent non-tumoral tissue ( n = 46, one per patient). Results are expressed as the percentage of low, medium, or high intensity of the staining. D Scoring of S100A10 immunohistochemistry performed on liver TMA from HCC patients comparing the staining intensity in the tumoral compartment versus the non-tumoral part for every patient. Results are expressed as the percentage of decreased (red), unchanged (white), or increased (green) staining intensity in the tumor in comparison to the non-tumoral liver. E Representative images of S100A10 staining (brown) in adjacent non-tumoral liver (left) and in HCC (right). Nuclei are counterstained with hematoxylin. Scale bar: 50 µm. F Scoring of S100A11 immunohistochemistry performed on liver TMA from HCC patients comparing HCC ( n = 92, two per patient) and adjacent non-tumoral tissue ( n = 46, one per patient). Results are expressed as the percentage of low, medium, or high intensity of the staining. G Scoring of S100A11 immunohistochemistry performed on liver TMA from HCC patients comparing the staining intensity in the tumoral compartment versus the non-tumoral part for every patient. Results are expressed as the percentage of decreased (red), unchanged (white), or increased (green) staining intensity in the tumor in comparison to the non-tumoral liver. H Representative images of S100A11 staining (brown) in adjacent non-tumoral liver (left) and in HCC (right). Nuclei are counterstained with hemalum. Scale bar: 50 µm. I Western blot analysis of S100A10 and S100A11 following FLAG immunoprecipitation of the cell lysates from Huh7 cells transfected with S100A10-FLAG (upper panel) or S100A11-FLAG (lower panel). Results are presented as means +/− S.E.M. “ n ” represents the number of animals ( A , B , and J ), the number of human liver biopsies ( C , D , F , and G ). * = p < 0.05; ** = p < 0.01; *** = p < 0.001 determined by One-Way ANOVA followed by Dunnett’s post-hoc analysis ( A , B ).

Article Snippet: S100A10 (1/2000, Abcam, ab76472), S100A11 (1/1000, Proteintech, ref 10237-1-AP), and tubulin (1/5000, Cell Signaling, ref 2128) antibodies were used prior HRP-conjugated secondary antibodies and chemiluminescence measurement (Fusion FX, Vilber).

Techniques: Western Blot, Injection, Immunohistochemistry, Staining, Comparison, Immunoprecipitation, Transfection