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TaKaRa 6 well plates
6 Well Plates, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/6 well plates/product/TaKaRa
Average 93 stars, based on 9 article reviews
Price from $9.99 to $1999.99
6 well plates - by Bioz Stars, 2020-09
93/100 stars

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Transduction:

Article Title: WHIM syndrome myelokathexis reproduced in the NOD/SCID mouse xenotransplant model engrafted with healthy human stem cells transduced with C-terminus-truncated CXCR4
Article Snippet: .. PBSCs from healthy human were transduced with RD114-MFGS vectors (multiplicity of infection [MOI], 5-15) overnight 3 times (days 1, 2, and 3) in culture medium containing 6 μg/mL protamine in 6-well plates precoated with RetroNectin (TaKaRa Bio, Otsu, Japan). .. PBSCs transduced with GFP only served as positive control for GFP expression but as a negative control for expression of wt CXCR4 or mutated CXCR4 transgenes.

Transfection:

Article Title: Nuclear Transport Factor 2 (NTF2) suppresses metastatic melanoma by modifying cell migration, metastasis, and gene expression
Article Snippet: .. NTF2-inducible melanoma cells WM983B cells were seeded into 6-well plates and transfected at 70% confluency using Xfect Transfection Reagent (#631317, Clontech) with 5 µg of pTetOne NTF2 (pDL66) and 100 ng of linear hygromycin marker (#631625, Clontech). .. 48 hours post transfection, confluent cells were split into 4 x 10 cm dishes.

Article Title: Nuclear Transport Factor 2 (NTF2) suppresses metastatic melanoma by modifying cell migration, metastasis, and gene expression
Article Snippet: .. NTF2-overexpressing prostate cancer cells LNCaP cells were seeded into 6-well plates and transfected at 70% confluency using Xfect Transfection Reagent (#631317, Clontech) with 5 µg of pEmCherry-C2 NTF2 (pDL23). .. 48 hours post transfection, confluent cells were split into 4 x 10 cm dishes.

Article Title: PTOV1 Enables the Nuclear Translocation and Mitogenic Activity of Flotillin-1, a Major Protein of Lipid Rafts
Article Snippet: .. For transfection of duplexed siRNAs, PC-3 cells plated at 2 × 105 cells/well in 6-well plates or 7 × 104 cells in coverslips were cotransfected with siRNA duplexes and pEGFP vector (0.1 μg/well) (Clontech) to monitor transfected cells using Lipofectamine Plus reagent (Invitrogen). .. Forty-eight hours after transfection, cells were lysed for Western blotting analysis or fixed with 4% paraformaldehyde for immunocytochemistry.

Article Title: Organic anion transporters also mediate the drug–drug interaction between imipenem and cilastatin
Article Snippet: .. 5 × 105 hOAT1- and hOAT3-HEK293 transfected cells and mock cells were seeded in each well of 6-well plates, and then the indicated drugs were added for 48 h. Total RNA was extracted by the RNA isoPlusⓇ Reagent Kit (Takara Biotechnology, Japan), and 1 µg total RNA was applied to reverse transcription for cDNA. cDNA was amplified and detected using the SYBRⓇ Premix Ex Taq™ Kit (Takara Biotechnology, Japan) by ABI PRISMⓇ 7500 Real-Time PCR System (Applied Biosystems, USA). .. Relative quantification of gene expression of DPEP1 was calculated by the 2−△△Ct method.

Amplification:

Article Title: Organic anion transporters also mediate the drug–drug interaction between imipenem and cilastatin
Article Snippet: .. 5 × 105 hOAT1- and hOAT3-HEK293 transfected cells and mock cells were seeded in each well of 6-well plates, and then the indicated drugs were added for 48 h. Total RNA was extracted by the RNA isoPlusⓇ Reagent Kit (Takara Biotechnology, Japan), and 1 µg total RNA was applied to reverse transcription for cDNA. cDNA was amplified and detected using the SYBRⓇ Premix Ex Taq™ Kit (Takara Biotechnology, Japan) by ABI PRISMⓇ 7500 Real-Time PCR System (Applied Biosystems, USA). .. Relative quantification of gene expression of DPEP1 was calculated by the 2−△△Ct method.

Isolation:

Article Title: Grape Seed Proanthocyanidins Induce Autophagy and Modulate Survivin in HepG2 Cells and Inhibit Xenograft Tumor Growth in Vivo
Article Snippet: .. RNA Isolation and Quantitative Real-Time Polymerase Chain Reaction (qPCR) Analysis The HepG2 cells were grown in 6-well plates, and the total RNA was extracted using Trizol (Takara, Dalian, China). cDNA was prepared according to the manufacturer’s instructions using a HiFiScript cDNA Synthesis Kit (Cwbio, Beijing, China). .. The amplification of GAPDH was used as the internal reference gene to normalize the expression of the selected genes.

Infection:

Article Title: WHIM syndrome myelokathexis reproduced in the NOD/SCID mouse xenotransplant model engrafted with healthy human stem cells transduced with C-terminus-truncated CXCR4
Article Snippet: .. PBSCs from healthy human were transduced with RD114-MFGS vectors (multiplicity of infection [MOI], 5-15) overnight 3 times (days 1, 2, and 3) in culture medium containing 6 μg/mL protamine in 6-well plates precoated with RetroNectin (TaKaRa Bio, Otsu, Japan). .. PBSCs transduced with GFP only served as positive control for GFP expression but as a negative control for expression of wt CXCR4 or mutated CXCR4 transgenes.

Real-time Polymerase Chain Reaction:

Article Title: Grape Seed Proanthocyanidins Induce Autophagy and Modulate Survivin in HepG2 Cells and Inhibit Xenograft Tumor Growth in Vivo
Article Snippet: .. RNA Isolation and Quantitative Real-Time Polymerase Chain Reaction (qPCR) Analysis The HepG2 cells were grown in 6-well plates, and the total RNA was extracted using Trizol (Takara, Dalian, China). cDNA was prepared according to the manufacturer’s instructions using a HiFiScript cDNA Synthesis Kit (Cwbio, Beijing, China). .. The amplification of GAPDH was used as the internal reference gene to normalize the expression of the selected genes.

Article Title: Organic anion transporters also mediate the drug–drug interaction between imipenem and cilastatin
Article Snippet: .. 5 × 105 hOAT1- and hOAT3-HEK293 transfected cells and mock cells were seeded in each well of 6-well plates, and then the indicated drugs were added for 48 h. Total RNA was extracted by the RNA isoPlusⓇ Reagent Kit (Takara Biotechnology, Japan), and 1 µg total RNA was applied to reverse transcription for cDNA. cDNA was amplified and detected using the SYBRⓇ Premix Ex Taq™ Kit (Takara Biotechnology, Japan) by ABI PRISMⓇ 7500 Real-Time PCR System (Applied Biosystems, USA). .. Relative quantification of gene expression of DPEP1 was calculated by the 2−△△Ct method.

Incubation:

Article Title: High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange
Article Snippet: .. Supernatants were harvested and filtered with 0.2 mM filters after 24 h incubation and transferred to Jurkat/MA cells in 6-well plates pre-treated with 1 mL well/Retronectin (20 mg/mL in PBS, Takara Bio. ..

Marker:

Article Title: Nuclear Transport Factor 2 (NTF2) suppresses metastatic melanoma by modifying cell migration, metastasis, and gene expression
Article Snippet: .. NTF2-inducible melanoma cells WM983B cells were seeded into 6-well plates and transfected at 70% confluency using Xfect Transfection Reagent (#631317, Clontech) with 5 µg of pTetOne NTF2 (pDL66) and 100 ng of linear hygromycin marker (#631625, Clontech). .. 48 hours post transfection, confluent cells were split into 4 x 10 cm dishes.

Plasmid Preparation:

Article Title: PTOV1 Enables the Nuclear Translocation and Mitogenic Activity of Flotillin-1, a Major Protein of Lipid Rafts
Article Snippet: .. For transfection of duplexed siRNAs, PC-3 cells plated at 2 × 105 cells/well in 6-well plates or 7 × 104 cells in coverslips were cotransfected with siRNA duplexes and pEGFP vector (0.1 μg/well) (Clontech) to monitor transfected cells using Lipofectamine Plus reagent (Invitrogen). .. Forty-eight hours after transfection, cells were lysed for Western blotting analysis or fixed with 4% paraformaldehyde for immunocytochemistry.

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  • 92
    TaKaRa xav939
    H446 cells were treated with different concentrations of <t>XAV939</t> for 24 h. (A) Western blotting analysis of β-catenin and cyclin D1 expression in H446 cells compared with β-actin. The expression of (B) β-catenin and (C) cyclin D1 is inhibited by XAV939 compared with the blank control. Each bar represents the mean ± standard deviation from three independent experiments. *P
    Xav939, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/xav939/product/TaKaRa
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    xav939 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    94
    TaKaRa pnf κb luc
    DEPDC7 depletion reduces NF-κB activation. A) HEK-293 cells were infected with retroviral vectors encoding for four different shRNAs targeting human DEPDC7 or a control shRNA (scramble). After selection, DEPDC7 mRNA levels normalized to GAPDH were quantified by real-time PCR. Right panel : protein lysates extracted from cells retrovirally expressing the indicated shRNA targeting DEPDC7 were analyzed for DEPDC7 expression by immunoblot assay B) Real-time PCR analysis of NF-κB target genes mRNAs in cells infected with retroviral vectors encoding shRNAs targeting human DEPDC7 or a control shRNA. Data shown indicates normalized expression level over scramble infected cells. C) HEK-293 cells depleted of DEPDC7 were transfected with <t>pNF-κB-luciferase</t> reporter plasmid and the indicated expression constructs. Data shown represents the relative luciferase activity normalized against β-galactosidase activity and is representative of at least 10 independent experiments performed in triplicate. Statistical analysis was performed by Student's t test; a p value of
    Pnf κb Luc, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pnf κb luc/product/TaKaRa
    Average 94 stars, based on 88 article reviews
    Price from $9.99 to $1999.99
    pnf κb luc - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    86
    TaKaRa plasmids expressing vzv orf47
    Phosphorylated H2AX and ATM levels in cells transfected with plasmids expressing <t>VZV</t> <t>ORF47,</t> ORF66, or combined ORF47 and ORF66. MeWo cells were transfected with 5ug of plasmid DNAs containing GFP, carboxyl epitope tagged VZV ORF47, ORF66, ORF47 and ORF66,
    Plasmids Expressing Vzv Orf47, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmids expressing vzv orf47/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmids expressing vzv orf47 - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    H446 cells were treated with different concentrations of XAV939 for 24 h. (A) Western blotting analysis of β-catenin and cyclin D1 expression in H446 cells compared with β-actin. The expression of (B) β-catenin and (C) cyclin D1 is inhibited by XAV939 compared with the blank control. Each bar represents the mean ± standard deviation from three independent experiments. *P

    Journal: Oncology Letters

    Article Title: Inhibitory effects of XAV939 on the proliferation of small-cell lung cancer H446 cells and Wnt/β-catenin signaling pathway in vitro

    doi: 10.3892/ol.2018.8790

    Figure Lengend Snippet: H446 cells were treated with different concentrations of XAV939 for 24 h. (A) Western blotting analysis of β-catenin and cyclin D1 expression in H446 cells compared with β-actin. The expression of (B) β-catenin and (C) cyclin D1 is inhibited by XAV939 compared with the blank control. Each bar represents the mean ± standard deviation from three independent experiments. *P

    Article Snippet: The N-H446 cells were plated in 6-well plates and treated with XAV939 (10, 20 and 40 µM) for 24 h. Total RNA extraction was performed according to the TRIZOL manufacturer's protocol (Takara Bio, Inc., Otsu, Japan).

    Techniques: Western Blot, Expressing, Standard Deviation

    Reverse transcription-quantitative polymerase chain reaction assay of (A) β-catenin and (B) cyclin D1 expression. H446 cells were treated with different concentrations of XAV939 for 24 h. The expression of β-catenin and cyclin D1 compared with the blank control. The expression of β-catenin and cyclin D1 is negatively regulated by XAV939. *P

    Journal: Oncology Letters

    Article Title: Inhibitory effects of XAV939 on the proliferation of small-cell lung cancer H446 cells and Wnt/β-catenin signaling pathway in vitro

    doi: 10.3892/ol.2018.8790

    Figure Lengend Snippet: Reverse transcription-quantitative polymerase chain reaction assay of (A) β-catenin and (B) cyclin D1 expression. H446 cells were treated with different concentrations of XAV939 for 24 h. The expression of β-catenin and cyclin D1 compared with the blank control. The expression of β-catenin and cyclin D1 is negatively regulated by XAV939. *P

    Article Snippet: The N-H446 cells were plated in 6-well plates and treated with XAV939 (10, 20 and 40 µM) for 24 h. Total RNA extraction was performed according to the TRIZOL manufacturer's protocol (Takara Bio, Inc., Otsu, Japan).

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    DEPDC7 depletion reduces NF-κB activation. A) HEK-293 cells were infected with retroviral vectors encoding for four different shRNAs targeting human DEPDC7 or a control shRNA (scramble). After selection, DEPDC7 mRNA levels normalized to GAPDH were quantified by real-time PCR. Right panel : protein lysates extracted from cells retrovirally expressing the indicated shRNA targeting DEPDC7 were analyzed for DEPDC7 expression by immunoblot assay B) Real-time PCR analysis of NF-κB target genes mRNAs in cells infected with retroviral vectors encoding shRNAs targeting human DEPDC7 or a control shRNA. Data shown indicates normalized expression level over scramble infected cells. C) HEK-293 cells depleted of DEPDC7 were transfected with pNF-κB-luciferase reporter plasmid and the indicated expression constructs. Data shown represents the relative luciferase activity normalized against β-galactosidase activity and is representative of at least 10 independent experiments performed in triplicate. Statistical analysis was performed by Student's t test; a p value of

    Journal: PLoS ONE

    Article Title: The Dishevelled, EGL-10 and Pleckstrin (DEP) Domain-Containing Protein DEPDC7 Binds to CARMA2 and CARMA3 Proteins, and Regulates NF-κB Activation

    doi: 10.1371/journal.pone.0116062

    Figure Lengend Snippet: DEPDC7 depletion reduces NF-κB activation. A) HEK-293 cells were infected with retroviral vectors encoding for four different shRNAs targeting human DEPDC7 or a control shRNA (scramble). After selection, DEPDC7 mRNA levels normalized to GAPDH were quantified by real-time PCR. Right panel : protein lysates extracted from cells retrovirally expressing the indicated shRNA targeting DEPDC7 were analyzed for DEPDC7 expression by immunoblot assay B) Real-time PCR analysis of NF-κB target genes mRNAs in cells infected with retroviral vectors encoding shRNAs targeting human DEPDC7 or a control shRNA. Data shown indicates normalized expression level over scramble infected cells. C) HEK-293 cells depleted of DEPDC7 were transfected with pNF-κB-luciferase reporter plasmid and the indicated expression constructs. Data shown represents the relative luciferase activity normalized against β-galactosidase activity and is representative of at least 10 independent experiments performed in triplicate. Statistical analysis was performed by Student's t test; a p value of

    Article Snippet: Luciferase Assay To assess for NF-κB activation, HEK-293 cells were plated in standard 6-well plates with with 0.2 µg of pNF-κB-luc (Clontech), 0.1 µg of pRSV-βGal (Addgene) plus each expression plasmid.

    Techniques: Activation Assay, Infection, shRNA, Selection, Real-time Polymerase Chain Reaction, Expressing, Transfection, Luciferase, Plasmid Preparation, Construct, Activity Assay

    DEPDC7 activates NF-κB. A) HEK-293 cells were co-transfected with pNF-κB-luciferase plasmid, a β-galactosidase reporter vector, and the indicated amount of an expression vector encoding for the DEPDC7. 24 hrs after transfection, cell lysates were prepared, and luciferase activity was measured. Data shown represent relative luciferase activity normalized against β-galactosidase activity and are representative of at least 10 independent experiments performed in triplicate. B) Real-time PCR analysis of IL-6 and CCL20 mRNAs in cells transfected with DEPDC7. Data shown indicates normalized fold induction over mock transfectant. Statistical analysis was performed by Student's t test; a p value of

    Journal: PLoS ONE

    Article Title: The Dishevelled, EGL-10 and Pleckstrin (DEP) Domain-Containing Protein DEPDC7 Binds to CARMA2 and CARMA3 Proteins, and Regulates NF-κB Activation

    doi: 10.1371/journal.pone.0116062

    Figure Lengend Snippet: DEPDC7 activates NF-κB. A) HEK-293 cells were co-transfected with pNF-κB-luciferase plasmid, a β-galactosidase reporter vector, and the indicated amount of an expression vector encoding for the DEPDC7. 24 hrs after transfection, cell lysates were prepared, and luciferase activity was measured. Data shown represent relative luciferase activity normalized against β-galactosidase activity and are representative of at least 10 independent experiments performed in triplicate. B) Real-time PCR analysis of IL-6 and CCL20 mRNAs in cells transfected with DEPDC7. Data shown indicates normalized fold induction over mock transfectant. Statistical analysis was performed by Student's t test; a p value of

    Article Snippet: Luciferase Assay To assess for NF-κB activation, HEK-293 cells were plated in standard 6-well plates with with 0.2 µg of pNF-κB-luc (Clontech), 0.1 µg of pRSV-βGal (Addgene) plus each expression plasmid.

    Techniques: Transfection, Luciferase, Plasmid Preparation, Expressing, Activity Assay, Real-time Polymerase Chain Reaction

    zBcl10 activates NF-κB in zebrafish cells. A) PAC2 cells were transiently cotransfected with expression vectors encoding for the indicated polypeptides, together with pNF-κB-luc and pRSV-βgal reporter vectors. The total amount of transfected plasmidic DNA was maintained constant by adding empty vector. 24 hrs after transfection, cell lysates were prepared and luciferase activity was measured. Data shown (mean + SEM, n = 9) represent relative luciferase activity normalized on β-galactosidase activity and is representative of six independent experiments done in triplicate. Statistical analysis was performed by Student's t test; a p value of

    Journal: PLoS ONE

    Article Title: Functional Characterization of Zebrafish (Danio rerio) Bcl10

    doi: 10.1371/journal.pone.0122365

    Figure Lengend Snippet: zBcl10 activates NF-κB in zebrafish cells. A) PAC2 cells were transiently cotransfected with expression vectors encoding for the indicated polypeptides, together with pNF-κB-luc and pRSV-βgal reporter vectors. The total amount of transfected plasmidic DNA was maintained constant by adding empty vector. 24 hrs after transfection, cell lysates were prepared and luciferase activity was measured. Data shown (mean + SEM, n = 9) represent relative luciferase activity normalized on β-galactosidase activity and is representative of six independent experiments done in triplicate. Statistical analysis was performed by Student's t test; a p value of

    Article Snippet: Luciferase and β-galactosidase assays To assess for NF-κB activation, HEK293 were co-transfected in 6-well plates with 0.2 μg of pNF-κB-luc (Clontech), 0.1 μg of pRSV-βGal (Addgene) plus each expression plasmid.

    Techniques: Expressing, Transfection, Plasmid Preparation, Luciferase, Activity Assay

    zBcl10 activates NF-κB. A) HEK293 cells were transiently cotransfected with an expression vector empy ( vector ) or encoding for the indicated polypeptides, together with pNF-κB-luc and pRSV-βgal reporter vectors. The total amount of transfected plasmidic DNA was maintained constant by adding empty vector. 16 hrs after transfection, cell lysates were prepared and luciferase activity was measured. In the bottom panels, a fraction of the cell lysates were analyzed by immunoblot to monitor protein expression. Data shown (mean + SEM, n = 9) represent relative luciferase activity normalized on β-galactosidase activity and is representative of six independent experiments done in triplicate. Statistical analysis was performed by Student's t test; a p value of

    Journal: PLoS ONE

    Article Title: Functional Characterization of Zebrafish (Danio rerio) Bcl10

    doi: 10.1371/journal.pone.0122365

    Figure Lengend Snippet: zBcl10 activates NF-κB. A) HEK293 cells were transiently cotransfected with an expression vector empy ( vector ) or encoding for the indicated polypeptides, together with pNF-κB-luc and pRSV-βgal reporter vectors. The total amount of transfected plasmidic DNA was maintained constant by adding empty vector. 16 hrs after transfection, cell lysates were prepared and luciferase activity was measured. In the bottom panels, a fraction of the cell lysates were analyzed by immunoblot to monitor protein expression. Data shown (mean + SEM, n = 9) represent relative luciferase activity normalized on β-galactosidase activity and is representative of six independent experiments done in triplicate. Statistical analysis was performed by Student's t test; a p value of

    Article Snippet: Luciferase and β-galactosidase assays To assess for NF-κB activation, HEK293 were co-transfected in 6-well plates with 0.2 μg of pNF-κB-luc (Clontech), 0.1 μg of pRSV-βGal (Addgene) plus each expression plasmid.

    Techniques: Expressing, Plasmid Preparation, Transfection, Luciferase, Activity Assay

    Phosphorylated H2AX and ATM levels in cells transfected with plasmids expressing VZV ORF47, ORF66, or combined ORF47 and ORF66. MeWo cells were transfected with 5ug of plasmid DNAs containing GFP, carboxyl epitope tagged VZV ORF47, ORF66, ORF47 and ORF66,

    Journal: Virology

    Article Title: Activation of H2AX and ATM in varicella-zoster virus (VZV)-infected cells is associated with expression of specific VZV genes

    doi: 10.1016/j.virol.2013.12.039

    Figure Lengend Snippet: Phosphorylated H2AX and ATM levels in cells transfected with plasmids expressing VZV ORF47, ORF66, or combined ORF47 and ORF66. MeWo cells were transfected with 5ug of plasmid DNAs containing GFP, carboxyl epitope tagged VZV ORF47, ORF66, ORF47 and ORF66,

    Article Snippet: HEK293T cells in 6 well plates were transfected with 2 µg of plasmids expressing VZV ORF47, ORF66, or ORF13 ( ) and 2 days later, the cells were lysed in RIPA buffer (0.01 M Tris HCl [pH 7.4], 0.15 M NaCl, 1% Triton X-100, 1% deoxycholate, 0.1% SDS) with complete protease inhibitors (Clontech) and 1 mM sodium vanadate to inhibit phosphatases.

    Techniques: Transfection, Expressing, Plasmid Preparation