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Sarstedt 6 well plates
6 Well Plates, supplied by Sarstedt, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/6 well plates/product/Sarstedt
Average 93 stars, based on 7 article reviews
Price from $9.99 to $1999.99
6 well plates - by Bioz Stars, 2020-09
93/100 stars

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Related Articles

Transfection:

Article Title: Polycystic Kidney Disease Ryanodine Receptor Domain (PKDRR) Proteins in Oomycetes
Article Snippet: .. For transient transfections, cells were either subcultured on 6-well plates (Sarstedt Ireland, Co. Wexford) or 35 mm glass-bottom bottom dishes, with 10 mm in diameter, 0 thickness coverslips (MatTek Life Sciences, Ashland, MA, USA). .. Cells were transfected using Lipofectamine LTX according to the manufacturer’s instructions, with a DNA concentration of 1 μg/mL found to be optimal for each well of a 6-well plate.

Article Title: Unprecedentedly efficient CUG initiation of an overlapping reading frame in POLG mRNA yields novel protein POLGARF
Article Snippet: .. For luciferase assays, HEK293T cells were transfected in half-area 96-well or 6-well plates (Sarstedt). .. For , and Supplementary Figs. 1-3, the following were added to each well: 25 ng of each pSV40-firefly vector, 5 ng control pSV40-Renilla vector plus 0.2 μl Lipofectamine 2000 in 25 μl OptiMEM (both from Thermo Fisher).

Luciferase:

Article Title: Unprecedentedly efficient CUG initiation of an overlapping reading frame in POLG mRNA yields novel protein POLGARF
Article Snippet: .. For luciferase assays, HEK293T cells were transfected in half-area 96-well or 6-well plates (Sarstedt). .. For , and Supplementary Figs. 1-3, the following were added to each well: 25 ng of each pSV40-firefly vector, 5 ng control pSV40-Renilla vector plus 0.2 μl Lipofectamine 2000 in 25 μl OptiMEM (both from Thermo Fisher).

Cell Culture:

Article Title: Development of loop-mediated isothermal amplification assay for detection of human coronavirus-NL63.
Article Snippet: .. Cells were cultured in 6-well plates (Sarstedt, Nümbrecht, Germany) at 37 • C with 5% CO 2 and were infected with HCoV-NL63 at TCID 50 0166-0934/$ -see front matter © 2011 Elsevier B.V. All rights reserved. doi:10.1016/j.jviromet.2011.04.024 of 400. ..

Article Title: Development of loop-mediated isothermal amplification assay for detection of human coronavirus-NL63
Article Snippet: .. Cells were cultured in 6-well plates (Sarstedt, Nümbrecht, Germany) at 37 °C with 5% CO2 and were infected with HCoV-NL63 at TCID50 of 400. ..

Article Title: Reprogrammed Cells Display Distinct Proteomic Signatures Associated with Colony Morphology Variability
Article Snippet: .. Maintenance of the Reprogrammed Cells The reprogrammed cell lines were cultured in 6-well plates (cat# 83.3920, Sarstedt), coated with Matrigel (cat# 354230, Corning). .. The cells were maintained in mTeSR™1 media, and media were changed every day.

Infection:

Article Title: Development of loop-mediated isothermal amplification assay for detection of human coronavirus-NL63.
Article Snippet: .. Cells were cultured in 6-well plates (Sarstedt, Nümbrecht, Germany) at 37 • C with 5% CO 2 and were infected with HCoV-NL63 at TCID 50 0166-0934/$ -see front matter © 2011 Elsevier B.V. All rights reserved. doi:10.1016/j.jviromet.2011.04.024 of 400. ..

Article Title: Development of loop-mediated isothermal amplification assay for detection of human coronavirus-NL63
Article Snippet: .. Cells were cultured in 6-well plates (Sarstedt, Nümbrecht, Germany) at 37 °C with 5% CO2 and were infected with HCoV-NL63 at TCID50 of 400. ..

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  • 93
    Sarstedt sub culturing 3t3 l1 cells
    Influence of CysLT1RA on <t>3T3-L1</t> adipocyte differentiation. (A) Protein expression of PPARγ in 3T3-L1 adipocytes differentiated in the presence of 0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin plus CysLT1RA or rosiglitazone (2 μM) or DMSO (w/o). One representative western blot out of three is shown. Photographs (B) and densitometric analysis of the lipid accumulation in 3T3-L1 adipocytes visualized by Oil Red O staining of the differentiated cells in the presence of montelukast and zafirlukast. Rosiglitazone and DMSO (w/o) were used as positive and negative control, respectively. One representative experiment out of three is shown. (C) WST-1 viability assay of <t>3T3-L1</t> cells treated with the compounds plus differentiation cocktail (0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin) for 48 h. Ins, Insulin; IBMX, isobutylmethylxanthine; Monte, montelukast; Rosi, rosiglitazone; w/o, without PPARγ agonist; Zafir, zafirlukast. Significant changes versus the untreated control are indicated with an asterisk. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001.
    Sub Culturing 3t3 L1 Cells, supplied by Sarstedt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sub culturing 3t3 l1 cells/product/Sarstedt
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sub culturing 3t3 l1 cells - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    91
    Sarstedt 6 well tissue culture plate
    Influence of CysLT1RA on <t>3T3-L1</t> adipocyte differentiation. (A) Protein expression of PPARγ in 3T3-L1 adipocytes differentiated in the presence of 0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin plus CysLT1RA or rosiglitazone (2 μM) or DMSO (w/o). One representative western blot out of three is shown. Photographs (B) and densitometric analysis of the lipid accumulation in 3T3-L1 adipocytes visualized by Oil Red O staining of the differentiated cells in the presence of montelukast and zafirlukast. Rosiglitazone and DMSO (w/o) were used as positive and negative control, respectively. One representative experiment out of three is shown. (C) WST-1 viability assay of <t>3T3-L1</t> cells treated with the compounds plus differentiation cocktail (0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin) for 48 h. Ins, Insulin; IBMX, isobutylmethylxanthine; Monte, montelukast; Rosi, rosiglitazone; w/o, without PPARγ agonist; Zafir, zafirlukast. Significant changes versus the untreated control are indicated with an asterisk. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001.
    6 Well Tissue Culture Plate, supplied by Sarstedt, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6 well tissue culture plate/product/Sarstedt
    Average 91 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    6 well tissue culture plate - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    93
    Sarstedt 6 well plates
    Influence of CysLT1RA on <t>3T3-L1</t> adipocyte differentiation. (A) Protein expression of PPARγ in 3T3-L1 adipocytes differentiated in the presence of 0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin plus CysLT1RA or rosiglitazone (2 μM) or DMSO (w/o). One representative western blot out of three is shown. Photographs (B) and densitometric analysis of the lipid accumulation in 3T3-L1 adipocytes visualized by Oil Red O staining of the differentiated cells in the presence of montelukast and zafirlukast. Rosiglitazone and DMSO (w/o) were used as positive and negative control, respectively. One representative experiment out of three is shown. (C) WST-1 viability assay of <t>3T3-L1</t> cells treated with the compounds plus differentiation cocktail (0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin) for 48 h. Ins, Insulin; IBMX, isobutylmethylxanthine; Monte, montelukast; Rosi, rosiglitazone; w/o, without PPARγ agonist; Zafir, zafirlukast. Significant changes versus the untreated control are indicated with an asterisk. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001.
    6 Well Plates, supplied by Sarstedt, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6 well plates/product/Sarstedt
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    6 well plates - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Influence of CysLT1RA on 3T3-L1 adipocyte differentiation. (A) Protein expression of PPARγ in 3T3-L1 adipocytes differentiated in the presence of 0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin plus CysLT1RA or rosiglitazone (2 μM) or DMSO (w/o). One representative western blot out of three is shown. Photographs (B) and densitometric analysis of the lipid accumulation in 3T3-L1 adipocytes visualized by Oil Red O staining of the differentiated cells in the presence of montelukast and zafirlukast. Rosiglitazone and DMSO (w/o) were used as positive and negative control, respectively. One representative experiment out of three is shown. (C) WST-1 viability assay of 3T3-L1 cells treated with the compounds plus differentiation cocktail (0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin) for 48 h. Ins, Insulin; IBMX, isobutylmethylxanthine; Monte, montelukast; Rosi, rosiglitazone; w/o, without PPARγ agonist; Zafir, zafirlukast. Significant changes versus the untreated control are indicated with an asterisk. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: Zafirlukast Is a Dual Modulator of Human Soluble Epoxide Hydrolase and Peroxisome Proliferator-Activated Receptor γ

    doi: 10.3389/fphar.2019.00263

    Figure Lengend Snippet: Influence of CysLT1RA on 3T3-L1 adipocyte differentiation. (A) Protein expression of PPARγ in 3T3-L1 adipocytes differentiated in the presence of 0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin plus CysLT1RA or rosiglitazone (2 μM) or DMSO (w/o). One representative western blot out of three is shown. Photographs (B) and densitometric analysis of the lipid accumulation in 3T3-L1 adipocytes visualized by Oil Red O staining of the differentiated cells in the presence of montelukast and zafirlukast. Rosiglitazone and DMSO (w/o) were used as positive and negative control, respectively. One representative experiment out of three is shown. (C) WST-1 viability assay of 3T3-L1 cells treated with the compounds plus differentiation cocktail (0.25 μM dexamethasone, 0.5 mM IBMX, 1 μg/ml insulin) for 48 h. Ins, Insulin; IBMX, isobutylmethylxanthine; Monte, montelukast; Rosi, rosiglitazone; w/o, without PPARγ agonist; Zafir, zafirlukast. Significant changes versus the untreated control are indicated with an asterisk. ∗ P ≤ 0.05, ∗∗ P ≤ 0.01, ∗∗∗ P ≤ 0.001.

    Article Snippet: Cell culture flasks (T75, Cell+, vented cap) and 6-well plates for sub-culturing 3T3-L1 cells were obtained from SARSTEDT (Nümbrecht, Germany).

    Techniques: Expressing, Western Blot, Staining, Negative Control, Viability Assay