Structured Review

Eppendorf AG 6 well plates
Cellular localizations of FSGS-associated ACTN4 are regulated by amino terminus phosphomimetics mutations. (A–C) Cells grown in <t>6-well</t> plate were transfected with indicated plasmids and then stained with rhodamine phalloidin for actin filaments and DAPI for nuclei. Green is GFP-tagged ACTN4. (A) is NR6WT fibroblasts and ( B,C ) are melanoma cell line WM1158. Scale bar , 20 μm. (D,E) Immunoblotting of GFP, actin and GAPDH in total cellular lysate, fraction of cytoplasm and cytoskeleton. All WT and indicated mutants are tagged with GFP at their C-terminus. Shown is the representative of three independent experiments.
6 Well Plates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/6 well plates/product/Eppendorf AG
Average 99 stars, based on 7 article reviews
Price from $9.99 to $1999.99
6 well plates - by Bioz Stars, 2020-09
99/100 stars

Images

1) Product Images from "Focal segmental glomerulosclerosis ACTN4 mutants binding to actin: regulation by phosphomimetic mutations"

Article Title: Focal segmental glomerulosclerosis ACTN4 mutants binding to actin: regulation by phosphomimetic mutations

Journal: Scientific Reports

doi: 10.1038/s41598-019-51825-2

Cellular localizations of FSGS-associated ACTN4 are regulated by amino terminus phosphomimetics mutations. (A–C) Cells grown in 6-well plate were transfected with indicated plasmids and then stained with rhodamine phalloidin for actin filaments and DAPI for nuclei. Green is GFP-tagged ACTN4. (A) is NR6WT fibroblasts and ( B,C ) are melanoma cell line WM1158. Scale bar , 20 μm. (D,E) Immunoblotting of GFP, actin and GAPDH in total cellular lysate, fraction of cytoplasm and cytoskeleton. All WT and indicated mutants are tagged with GFP at their C-terminus. Shown is the representative of three independent experiments.
Figure Legend Snippet: Cellular localizations of FSGS-associated ACTN4 are regulated by amino terminus phosphomimetics mutations. (A–C) Cells grown in 6-well plate were transfected with indicated plasmids and then stained with rhodamine phalloidin for actin filaments and DAPI for nuclei. Green is GFP-tagged ACTN4. (A) is NR6WT fibroblasts and ( B,C ) are melanoma cell line WM1158. Scale bar , 20 μm. (D,E) Immunoblotting of GFP, actin and GAPDH in total cellular lysate, fraction of cytoplasm and cytoskeleton. All WT and indicated mutants are tagged with GFP at their C-terminus. Shown is the representative of three independent experiments.

Techniques Used: Transfection, Staining

2) Product Images from "Single molecule poly(A) tail-seq shows LARP4 opposes deadenylation throughout mRNA lifespan with most impact on short tails"

Article Title: Single molecule poly(A) tail-seq shows LARP4 opposes deadenylation throughout mRNA lifespan with most impact on short tails

Journal: eLife

doi: 10.7554/eLife.59186

LARP4 promotes accumulation of interferon-induced innate immune mRNAs. ( A ) Northern blot analysis of two ISG mRNAs, IFIT1 and ISG15 after transfection-mediated induction by IFN-stimulating nucleic acids, and + / - co transfection with LARP4, as indicated above the lanes. Bottom panels are stained gels prior to transfer. ( B ) Quantitation of biological triplicate northern blot data, normalized by GAPDH, with the poly(I:C) +, LARP4 - sample (annotated with # above the bar) set to 100%; error bars represent 95% confidence interval. P values were calculated using a 2-tailed Welch's T-test with unequal variance. ( C ) Northern blot of IFIT1 and LARP4 from total RNA 48 hr after transfection of 0, 2, 4 or 6 µg of 1 kb AT-rich DNA in 6-well format (the total DNA amount for this component of the transfection was maintained at 6 µg with carrier pUC19), plus 2.5 µg pFLAG-LARP4 (+) or empty pFLAG vector ( - ) as indicated. ( D ) Top: schematic of LARP4 showing its two PABP-interaction motifs, PAM2w and PBM, the La-module comprised of LaM (La motif) and RRM-L4, and the RACK-1 interaction region (RIR). The N-terminal region (NTR) which is responsible for poly(A)-binding ( Cruz-Gallardo et al., 2019 ), is also indicated. Middle: Northern blot after co-transfection of 1 kb AT-rich DNA or pUC19 and various LARP4 constructs indicated above the lanes. Bottom: Western blot analysis of protein from the same cells.
Figure Legend Snippet: LARP4 promotes accumulation of interferon-induced innate immune mRNAs. ( A ) Northern blot analysis of two ISG mRNAs, IFIT1 and ISG15 after transfection-mediated induction by IFN-stimulating nucleic acids, and + / - co transfection with LARP4, as indicated above the lanes. Bottom panels are stained gels prior to transfer. ( B ) Quantitation of biological triplicate northern blot data, normalized by GAPDH, with the poly(I:C) +, LARP4 - sample (annotated with # above the bar) set to 100%; error bars represent 95% confidence interval. P values were calculated using a 2-tailed Welch's T-test with unequal variance. ( C ) Northern blot of IFIT1 and LARP4 from total RNA 48 hr after transfection of 0, 2, 4 or 6 µg of 1 kb AT-rich DNA in 6-well format (the total DNA amount for this component of the transfection was maintained at 6 µg with carrier pUC19), plus 2.5 µg pFLAG-LARP4 (+) or empty pFLAG vector ( - ) as indicated. ( D ) Top: schematic of LARP4 showing its two PABP-interaction motifs, PAM2w and PBM, the La-module comprised of LaM (La motif) and RRM-L4, and the RACK-1 interaction region (RIR). The N-terminal region (NTR) which is responsible for poly(A)-binding ( Cruz-Gallardo et al., 2019 ), is also indicated. Middle: Northern blot after co-transfection of 1 kb AT-rich DNA or pUC19 and various LARP4 constructs indicated above the lanes. Bottom: Western blot analysis of protein from the same cells.

Techniques Used: Northern Blot, Transfection, Cotransfection, Staining, Quantitation Assay, Plasmid Preparation, Laser Capture Microdissection, Binding Assay, Construct, Western Blot

Related Articles

Transfection:

Article Title: ASH2L drives proliferation and sensitivity to bleomycin and other genotoxins in Hodgkin’s lymphoma and testicular cancer cells
Article Snippet: .. siRNA transfection U2OS cells were plated in 1 ml of DMEM at 4·104 per well in 6-well plates. .. The first round of siRNA transfection was performed right after the plating when the cells were still floating.

other:

Article Title: Single molecule poly(A) tail-seq shows LARP4 opposes deadenylation throughout mRNA lifespan with most impact on short tails
Article Snippet: IFNα and actinomycin D time course Two LARP4 WT and two KO MEF cell lines immortalized by the 3T3 method , were seeded at a density of 2.5 × 105 cells per well in 2 ml media with 10% FBS in 6-well plates; at least four wells per time point were seeded.

Article Title: Metabolic analysis reveals evidence for branched chain amino acid catabolism crosstalk and the potential for improved treatment of organic acidurias
Article Snippet: On day 1, cells were treated with trypsin, counted, and seeded into three 6-well plates at approximately 150,000 cells/well in complete DMEM media with 5% FBS.

Article Title: A systematic genetic interaction map of human solute carriers assigns a role to SLC25A51/MCART1 in mitochondrial NAD uptake
Article Snippet: Targeted metabolomics Cells were plated at 0.8-0.9×106 cells/well in 6-well plates in full media.

Incubation:

Article Title: Reversal of cisplatin resistance in human gastric cancer cells by a wogonin-conjugated Pt(IV) prodrug via attenuating Casein Kinase 2-mediated Nuclear Factor-κB pathways.
Article Snippet: .. Pt(IV) prodrugs, with two additional coordination sites in contrast to Pt(II) drugs, have been actively studied nowadays, for they can perform well in enhancing the accumulation and retention of the corresponding Pt(II) drugs in cancer cells. ..

Labeling:

Article Title: Exo-MFA – A 13C metabolic flux analysis to dissect tumor microenvironment-secreted exosome contributions towards cancer cell metabolism
Article Snippet: .. Intracellular metabolites extraction: Cancer cells were seeded in 6-well plates overnight, and replaced with medium containing 13C labeled CDEs. ..

Expressing:

Article Title: Recombinant Lactococcus lactis expressing bioactive exendin-4 to promote insulin secretion and beta-cell proliferation in vitro.
Article Snippet: .. In recent years, therapeutic peptides have garnered great interest in the pharmaceutical industry for the treatment of diabetes. ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Eppendorf AG p aeruginosa
    P. <t>aeruginosa</t> ExoS ADPr inhibits EGF-activated EGFR trafficking to the lysosome
    P Aeruginosa, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p aeruginosa/product/Eppendorf AG
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    p aeruginosa - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    93
    Eppendorf AG 6 well plates
    Cellular localizations of FSGS-associated ACTN4 are regulated by amino terminus phosphomimetics mutations. (A–C) Cells grown in <t>6-well</t> plate were transfected with indicated plasmids and then stained with rhodamine phalloidin for actin filaments and DAPI for nuclei. Green is GFP-tagged ACTN4. (A) is NR6WT fibroblasts and ( B,C ) are melanoma cell line WM1158. Scale bar , 20 μm. (D,E) Immunoblotting of GFP, actin and GAPDH in total cellular lysate, fraction of cytoplasm and cytoskeleton. All WT and indicated mutants are tagged with GFP at their C-terminus. Shown is the representative of three independent experiments.
    6 Well Plates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6 well plates/product/Eppendorf AG
    Average 93 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    6 well plates - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    99
    Eppendorf AG 12 well cell culture plates
    Schematic illustration of experimental design. (a) IPEC-J2 monocultures were grown as a monolayer on the top surface of Transwell cell culture inserts. Bacteria were added to the IPEC-J2 compartment. (b) MoDC monocultures were cultivated in <t>12-well</t> cell culture plates. Bacteria were added to the MoDC compartment. (c)–(d) Cocultures of IPEC-J2 cells grown on Transwell inserts and adherent MoDC located in the bottom compartment. In separate approaches, bacteria were added either (c) to the IPEC-J2 compartment or (d) to the MoDC compartment. The lightning flash indicates the localization of the bacterial challenge with either E. faecium or ETEC.
    12 Well Cell Culture Plates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/12 well cell culture plates/product/Eppendorf AG
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    12 well cell culture plates - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    90
    Eppendorf AG 6 well tissue culture dishes
    Cells demonstrate a decreased level of Nectin-1 receptor expression after primary infection. Monolayers of HCE cells plated on a <t>6-well</t> tissue culture dish were treated with a viral dose at an MOI of 0.01 and incubated for 48 h at 37 °C. Cells were then treated with MTT to distinguish live from dead cells. FACS was then performed to test levels of Nectin-1 on living infected cells tagged with red fluorescent protein. HCE cells untreated with a viral dose were used as a control and are shown as the green shade.
    6 Well Tissue Culture Dishes, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6 well tissue culture dishes/product/Eppendorf AG
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    6 well tissue culture dishes - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    Image Search Results


    P. aeruginosa ExoS ADPr inhibits EGF-activated EGFR trafficking to the lysosome

    Journal: Traffic (Copenhagen, Denmark)

    Article Title: Modulation of host cell endocytosis by the type-III cytotoxin, Pseudomonas ExoS

    doi: 10.1111/j.1600-0854.2008.00808.x

    Figure Lengend Snippet: P. aeruginosa ExoS ADPr inhibits EGF-activated EGFR trafficking to the lysosome

    Article Snippet: HeLa cells, in 6 well plates, were co-cultured with P. aeruginosa expressing ExoS derived constructs at 37°C for 3 h. Cells were then washed and cultured in medium containing 300 μg/ml Gentamycin for 1 h. To ensure that invasion assays were not affected by loss of rounded, non-adherent cells, the supernatant of each well was collected in an Eppendorf tube.

    Techniques:

    P. aeruginosa ExoS ADPr inhibits HRP uptake

    Journal: Traffic (Copenhagen, Denmark)

    Article Title: Modulation of host cell endocytosis by the type-III cytotoxin, Pseudomonas ExoS

    doi: 10.1111/j.1600-0854.2008.00808.x

    Figure Lengend Snippet: P. aeruginosa ExoS ADPr inhibits HRP uptake

    Article Snippet: HeLa cells, in 6 well plates, were co-cultured with P. aeruginosa expressing ExoS derived constructs at 37°C for 3 h. Cells were then washed and cultured in medium containing 300 μg/ml Gentamycin for 1 h. To ensure that invasion assays were not affected by loss of rounded, non-adherent cells, the supernatant of each well was collected in an Eppendorf tube.

    Techniques:

    P. aeruginosa ExoS ADPr inhibits EGF-activated EGFR degradation

    Journal: Traffic (Copenhagen, Denmark)

    Article Title: Modulation of host cell endocytosis by the type-III cytotoxin, Pseudomonas ExoS

    doi: 10.1111/j.1600-0854.2008.00808.x

    Figure Lengend Snippet: P. aeruginosa ExoS ADPr inhibits EGF-activated EGFR degradation

    Article Snippet: HeLa cells, in 6 well plates, were co-cultured with P. aeruginosa expressing ExoS derived constructs at 37°C for 3 h. Cells were then washed and cultured in medium containing 300 μg/ml Gentamycin for 1 h. To ensure that invasion assays were not affected by loss of rounded, non-adherent cells, the supernatant of each well was collected in an Eppendorf tube.

    Techniques:

    P. aeruginosa ExoS ADPr does not inhibit EGF-activated EGFR internalization

    Journal: Traffic (Copenhagen, Denmark)

    Article Title: Modulation of host cell endocytosis by the type-III cytotoxin, Pseudomonas ExoS

    doi: 10.1111/j.1600-0854.2008.00808.x

    Figure Lengend Snippet: P. aeruginosa ExoS ADPr does not inhibit EGF-activated EGFR internalization

    Article Snippet: HeLa cells, in 6 well plates, were co-cultured with P. aeruginosa expressing ExoS derived constructs at 37°C for 3 h. Cells were then washed and cultured in medium containing 300 μg/ml Gentamycin for 1 h. To ensure that invasion assays were not affected by loss of rounded, non-adherent cells, the supernatant of each well was collected in an Eppendorf tube.

    Techniques:

    DA-Rab5 rescues P. aeruginosa ExoS ADPr inhibition of EGF-activated EGFR trafficking in the early endosome

    Journal: Traffic (Copenhagen, Denmark)

    Article Title: Modulation of host cell endocytosis by the type-III cytotoxin, Pseudomonas ExoS

    doi: 10.1111/j.1600-0854.2008.00808.x

    Figure Lengend Snippet: DA-Rab5 rescues P. aeruginosa ExoS ADPr inhibition of EGF-activated EGFR trafficking in the early endosome

    Article Snippet: HeLa cells, in 6 well plates, were co-cultured with P. aeruginosa expressing ExoS derived constructs at 37°C for 3 h. Cells were then washed and cultured in medium containing 300 μg/ml Gentamycin for 1 h. To ensure that invasion assays were not affected by loss of rounded, non-adherent cells, the supernatant of each well was collected in an Eppendorf tube.

    Techniques: Inhibition

    P. aeruginosa ExoS inhibition of bacterial internalization by HeLa cells is RhoGAP dependent

    Journal: Traffic (Copenhagen, Denmark)

    Article Title: Modulation of host cell endocytosis by the type-III cytotoxin, Pseudomonas ExoS

    doi: 10.1111/j.1600-0854.2008.00808.x

    Figure Lengend Snippet: P. aeruginosa ExoS inhibition of bacterial internalization by HeLa cells is RhoGAP dependent

    Article Snippet: HeLa cells, in 6 well plates, were co-cultured with P. aeruginosa expressing ExoS derived constructs at 37°C for 3 h. Cells were then washed and cultured in medium containing 300 μg/ml Gentamycin for 1 h. To ensure that invasion assays were not affected by loss of rounded, non-adherent cells, the supernatant of each well was collected in an Eppendorf tube.

    Techniques: Inhibition

    Cellular localizations of FSGS-associated ACTN4 are regulated by amino terminus phosphomimetics mutations. (A–C) Cells grown in 6-well plate were transfected with indicated plasmids and then stained with rhodamine phalloidin for actin filaments and DAPI for nuclei. Green is GFP-tagged ACTN4. (A) is NR6WT fibroblasts and ( B,C ) are melanoma cell line WM1158. Scale bar , 20 μm. (D,E) Immunoblotting of GFP, actin and GAPDH in total cellular lysate, fraction of cytoplasm and cytoskeleton. All WT and indicated mutants are tagged with GFP at their C-terminus. Shown is the representative of three independent experiments.

    Journal: Scientific Reports

    Article Title: Focal segmental glomerulosclerosis ACTN4 mutants binding to actin: regulation by phosphomimetic mutations

    doi: 10.1038/s41598-019-51825-2

    Figure Lengend Snippet: Cellular localizations of FSGS-associated ACTN4 are regulated by amino terminus phosphomimetics mutations. (A–C) Cells grown in 6-well plate were transfected with indicated plasmids and then stained with rhodamine phalloidin for actin filaments and DAPI for nuclei. Green is GFP-tagged ACTN4. (A) is NR6WT fibroblasts and ( B,C ) are melanoma cell line WM1158. Scale bar , 20 μm. (D,E) Immunoblotting of GFP, actin and GAPDH in total cellular lysate, fraction of cytoplasm and cytoskeleton. All WT and indicated mutants are tagged with GFP at their C-terminus. Shown is the representative of three independent experiments.

    Article Snippet: In brief, cells grown and transfected in 6-well plates were treated with trypsin and harvested in an Eppendorf tube.

    Techniques: Transfection, Staining

    Schematic illustration of experimental design. (a) IPEC-J2 monocultures were grown as a monolayer on the top surface of Transwell cell culture inserts. Bacteria were added to the IPEC-J2 compartment. (b) MoDC monocultures were cultivated in 12-well cell culture plates. Bacteria were added to the MoDC compartment. (c)–(d) Cocultures of IPEC-J2 cells grown on Transwell inserts and adherent MoDC located in the bottom compartment. In separate approaches, bacteria were added either (c) to the IPEC-J2 compartment or (d) to the MoDC compartment. The lightning flash indicates the localization of the bacterial challenge with either E. faecium or ETEC.

    Journal: Mediators of Inflammation

    Article Title: The Inflammatory Response to Enterotoxigenic E. coli and Probiotic E. faecium in a Coculture Model of Porcine Intestinal Epithelial and Dendritic Cells

    doi: 10.1155/2018/9368295

    Figure Lengend Snippet: Schematic illustration of experimental design. (a) IPEC-J2 monocultures were grown as a monolayer on the top surface of Transwell cell culture inserts. Bacteria were added to the IPEC-J2 compartment. (b) MoDC monocultures were cultivated in 12-well cell culture plates. Bacteria were added to the MoDC compartment. (c)–(d) Cocultures of IPEC-J2 cells grown on Transwell inserts and adherent MoDC located in the bottom compartment. In separate approaches, bacteria were added either (c) to the IPEC-J2 compartment or (d) to the MoDC compartment. The lightning flash indicates the localization of the bacterial challenge with either E. faecium or ETEC.

    Article Snippet: Cells were seeded at a density of 1.44 × 106 cells/ml and 1 ml per well in 12-well cell culture plates (TPP, Faust Lab, Klettgau, Germany or Eppendorf GmbH, Hamburg, Germany).

    Techniques: Cell Culture

    Cells demonstrate a decreased level of Nectin-1 receptor expression after primary infection. Monolayers of HCE cells plated on a 6-well tissue culture dish were treated with a viral dose at an MOI of 0.01 and incubated for 48 h at 37 °C. Cells were then treated with MTT to distinguish live from dead cells. FACS was then performed to test levels of Nectin-1 on living infected cells tagged with red fluorescent protein. HCE cells untreated with a viral dose were used as a control and are shown as the green shade.

    Journal: Molecular Vision

    Article Title: HSV-1 infection of human corneal epithelial cells: Receptor-mediated entry and trends of re-infection

    doi:

    Figure Lengend Snippet: Cells demonstrate a decreased level of Nectin-1 receptor expression after primary infection. Monolayers of HCE cells plated on a 6-well tissue culture dish were treated with a viral dose at an MOI of 0.01 and incubated for 48 h at 37 °C. Cells were then treated with MTT to distinguish live from dead cells. FACS was then performed to test levels of Nectin-1 on living infected cells tagged with red fluorescent protein. HCE cells untreated with a viral dose were used as a control and are shown as the green shade.

    Article Snippet: Monolayers of HCE cells plated on 6-well tissue culture dishes were transferred to 1.5 ml Eppendorf tubes using Hank's based enzyme-free cell dissociation buffer (Invitrogen) and were then washed three times for 10 min with a cold solution of 1× PBS with 10% glucose and 10% calf serum.

    Techniques: Expressing, Infection, Incubation, MTT Assay, FACS