5 6 fam se  (Thermo Fisher)


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    Name:
    5 6 FAM SE 5 and 6 Carboxyfluorescein Succinimidyl Ester
    Description:
    Searching for superior alternatives to fluorescein Our Alexa Fluor Dye Series offers everything you re looking for and more
    Catalog Number:
    c1311
    Price:
    None
    Applications:
    Amine-Reactive Fluorophores|Cell Analysis|Labeling Amines|Labeling Proteins & Antibodies with Fluorophores|Protein Biology|Protein Labeling|Protein Labeling & Crosslinking|Protein and Antibody Labeling|Protein, Peptide & Antibody Labeling|Labeling Chemistry
    Category:
    Labeling Detection Products
    Buy from Supplier


    Structured Review

    Thermo Fisher 5 6 fam se
    Searching for superior alternatives to fluorescein Our Alexa Fluor Dye Series offers everything you re looking for and more
    https://www.bioz.com/result/5 6 fam se/product/Thermo Fisher
    Average 90 stars, based on 83 article reviews
    Price from $9.99 to $1999.99
    5 6 fam se - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Antibodies to the Iron Uptake ABC Transporter Lipoproteins PiaA and PiuA Promote Opsonophagocytosis of Streptococcus pneumoniae
    Article Snippet: S. pneumoniae strains were fluorescently labeled by incubation with 5,6-carboxyfluorescein-succinimidyl ester (FAM-SE; Molecular Probes, Eugene, Oreg.) solution (10 mg/ml in dimethyl sulfoxide; Sigma) in 0.1 M sodium bicarbonate buffer for 1 h at 37°C and then washed six times with Hanks balanced salt solution (HBSS) in 0.2% bovine serum albumin and stored in aliquots at −70°C in 10% glycerol (approximately 109 CFU/ml). .. Differentiated HL60 cells were harvested by centrifugation (160 × g , 8 min, 4°C) and washed twice with HBSS and once with HBSS in the presence of Ca2+ and Mg2+ .

    Article Title: Eradication of Cryptosporidium parvum Infection by Mice with Ovalbumin-Specific T Cells
    Article Snippet: CFSE (catalog no. C-1311; Molecular Probes, Eugene, Oreg.), stored as a 10 mM stock solution in dimethyl sulfoxide, was diluted to 4 μM in 10 ml of saline at 37°C, and 4 × 107 lymphocytes were added in 10 ml of saline. .. After 10 min at 37°C, the cells were pelleted by centrifugation, resuspended in sterile HBSS, and injected i.p.

    Amplification:

    Article Title: L-arginine-NO-cGMP signalling pathway in pancreatitis
    Article Snippet: The HRP label was amplified with FITC-conjugated tyramine. .. FITC-tyramine conjugate was synthesised by us from tyramine-HCl (Sigma) and FITC-succinimidyl ester (FITC-NHS, #C1311 from Molecular Probes) in DMF.

    Synthesized:

    Article Title: Inhibition of osteoblast mineralization by phosphorylated phage-derived apatite-specific peptide
    Article Snippet: Peptides were synthesized using Fmoc solid-phase chemistry according to standard peptide synthesis procedures and characterized as having > 90 % purity by high-performance liquid chromatography (University of Michigan Proteomics & Peptide Synthesis Core). .. For experiments involving fluorescence, peptides were labelled on resin before cleavage using 5-(and-6)-carboxyfluorescein succinimidyl ester (Molecular Probes C-1311).

    Cytometry:

    Article Title: Technical Advance: Soluble OX40 molecule mimics regulatory T cell modulatory activity on Fc?RI-dependent mast cell degranulation
    Article Snippet: DMSO-resuspended 5-(and-6)-CFSE/FAM (150 μg; C-1311; Invitrogen, Carlsbad, CA, USA) was added to 1 mg sOX40 for 90 min at 4°C. .. WT or Tnfsf4 −/− BMMCs were incubated with sOX40-CFSE (20 μg/ml) for 30 min at room temperature, washed, and analyzed by flow cytometry.

    Article Title: Antibodies to the Iron Uptake ABC Transporter Lipoproteins PiaA and PiuA Promote Opsonophagocytosis of Streptococcus pneumoniae
    Article Snippet: To assess the effect of anti-PiuA and anti-PiaA antibodies on the interaction of S. pneumoniae with phagocytes, we measured the proportion of a neutrophil cell line associated with fluorescent bacteria by using a previously described flow cytometry opsonophagocytosis assay ( , , ). .. S. pneumoniae strains were fluorescently labeled by incubation with 5,6-carboxyfluorescein-succinimidyl ester (FAM-SE; Molecular Probes, Eugene, Oreg.) solution (10 mg/ml in dimethyl sulfoxide; Sigma) in 0.1 M sodium bicarbonate buffer for 1 h at 37°C and then washed six times with Hanks balanced salt solution (HBSS) in 0.2% bovine serum albumin and stored in aliquots at −70°C in 10% glycerol (approximately 109 CFU/ml).

    Article Title: Serum Amyloid P Aids Complement-Mediated Immunity to Streptococcus pneumoniae
    Article Snippet: Phagocytosis of S. pneumoniae in serum from Apcs−/− , Apcs−/− .C1qa−/− , and wild-type mice was investigated using a previously described flow cytometry assay and the human tissue culture cell line HL-60 (promyelocytic leukemia cells; CCL240; American Type Culture Collection, http://www.atcc.org/ ) differentiated into granulocytes [ , ]. .. S. pneumoniae were fluorescently labelled with 5,6-carboxyfluorescein succinimidyl ester (FAM-SE; Molecular Probes, http://probes.invitrogen.com/ ) as described and stored at −70 °C in 10% glycerol as single-use aliquots (7 × 108 cfu/ml).

    Microarray:

    Article Title: Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1 2
    Article Snippet: .. To derive a gene expression signature of C1311 sensitivity, we began by selecting candidate sensitivity biomarkers by rank-based correlation ( r s = 0.4) of C1311 IC50 values across the NCI-60 panel, finding that 219/22283 Affymetrix HG-U133A microarray probes meet this criterion (false discovery rate = 0.1 by random permutation testing, for probe information and correlation coefficients, see ). .. Evaluation of the potential functional associations of these 219 probe sets using the Ingenuity Pathway Analysis program identified the glycerophospholipid metabolism pathway as the most significantly enriched, supporting in cancer cells our observations associating C1311 with lipid biogenesis in budding yeast.

    Incubation:

    Article Title: Technical Advance: Soluble OX40 molecule mimics regulatory T cell modulatory activity on Fc?RI-dependent mast cell degranulation
    Article Snippet: DMSO-resuspended 5-(and-6)-CFSE/FAM (150 μg; C-1311; Invitrogen, Carlsbad, CA, USA) was added to 1 mg sOX40 for 90 min at 4°C. .. WT or Tnfsf4 −/− BMMCs were incubated with sOX40-CFSE (20 μg/ml) for 30 min at room temperature, washed, and analyzed by flow cytometry.

    Article Title: Tunable Thermal-Sensitive Polymer-Graphene Oxide Composite for Efficient Capture and Release of Viable Circulating Tumor Cells
    Article Snippet: .. To show the surface availability of the amine groups from the GO-PEG in polymer-GO composite films, the drop-cast films were incubated with 0.25mM aqueous solution of an amine reactive dye, FSE (5-(and-6)-carboxyfluorescein, succinimidyl ester; Life Technologies (C1311)) for 30 minutes at 40°C and then washed with copious amount of DI water. .. The dye treated films were then imaged with fluorescence microscope (Olympus BX51 coupled with Olympus DP71 camera and EXFO X-cite Series 120 light source).

    Article Title: Antibodies to the Iron Uptake ABC Transporter Lipoproteins PiaA and PiuA Promote Opsonophagocytosis of Streptococcus pneumoniae
    Article Snippet: .. S. pneumoniae strains were fluorescently labeled by incubation with 5,6-carboxyfluorescein-succinimidyl ester (FAM-SE; Molecular Probes, Eugene, Oreg.) solution (10 mg/ml in dimethyl sulfoxide; Sigma) in 0.1 M sodium bicarbonate buffer for 1 h at 37°C and then washed six times with Hanks balanced salt solution (HBSS) in 0.2% bovine serum albumin and stored in aliquots at −70°C in 10% glycerol (approximately 109 CFU/ml). .. The human cell line HL-60 (promyelocytic leukemia cells; CCL240; American Type Culture Collection, Manassas, Va.) was used to provide the effector cells after differentiation into granulocytes by using previously described protocols ( ).

    Article Title: Identification of ANXA2 (annexin A2) as a specific bleomycin target to induce pulmonary fibrosis by impeding TFEB-mediated autophagic flux
    Article Snippet: .. Briefly, 2.1 mM 5-(and-6)-carboxyfluorescein, succinimidyl ester (5(6)-FAM, SE (Thermo Fisher Scientific, C1311) was incubated with 0.6 mM bleomycin A5 in 0.2 M NaHCO3 , pH 8.3, at 4ºC overnight. ..

    Article Title: Serum Amyloid P Aids Complement-Mediated Immunity to Streptococcus pneumoniae
    Article Snippet: S. pneumoniae were fluorescently labelled with 5,6-carboxyfluorescein succinimidyl ester (FAM-SE; Molecular Probes, http://probes.invitrogen.com/ ) as described and stored at −70 °C in 10% glycerol as single-use aliquots (7 × 108 cfu/ml). .. HL-60 cells (105 ) were added to each well and incubated for 30 min at 37 °C, fixed with 3% PFA, and analysed using a FACScalibur flow cytometer (minimum of 6,000 cells per sample) to identify the proportion of cells associated with fluorescent bacteria as a marker of phagocytosis [ ].

    Article Title: L-arginine-NO-cGMP signalling pathway in pancreatitis
    Article Snippet: FITC-tyramine conjugate was synthesised by us from tyramine-HCl (Sigma) and FITC-succinimidyl ester (FITC-NHS, #C1311 from Molecular Probes) in DMF. .. Incubation with FITC-tyramine conjugate was carried out at a dilution of 1:500 in PBS in the presence of 0.02% H2 02 for 3–5 min.

    Expressing:

    Article Title: Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1 2
    Article Snippet: .. To derive a gene expression signature of C1311 sensitivity, we began by selecting candidate sensitivity biomarkers by rank-based correlation ( r s = 0.4) of C1311 IC50 values across the NCI-60 panel, finding that 219/22283 Affymetrix HG-U133A microarray probes meet this criterion (false discovery rate = 0.1 by random permutation testing, for probe information and correlation coefficients, see ). .. Evaluation of the potential functional associations of these 219 probe sets using the Ingenuity Pathway Analysis program identified the glycerophospholipid metabolism pathway as the most significantly enriched, supporting in cancer cells our observations associating C1311 with lipid biogenesis in budding yeast.

    Modification:

    Article Title: Tunable Thermal-Sensitive Polymer-Graphene Oxide Composite for Efficient Capture and Release of Viable Circulating Tumor Cells
    Article Snippet: In the first step, the polymer-GO composite film was deposited on a patterned and surface modified glass substrate followed by assembly with a PDMS chamber to form a microfluidic device. .. To show the surface availability of the amine groups from the GO-PEG in polymer-GO composite films, the drop-cast films were incubated with 0.25mM aqueous solution of an amine reactive dye, FSE (5-(and-6)-carboxyfluorescein, succinimidyl ester; Life Technologies (C1311)) for 30 minutes at 40°C and then washed with copious amount of DI water.

    Competitive Binding Assay:

    Article Title: Technical Advance: Soluble OX40 molecule mimics regulatory T cell modulatory activity on Fc?RI-dependent mast cell degranulation
    Article Snippet: Paragraph title: sOX40 fluorescein labeling, binding, and competition assay ... DMSO-resuspended 5-(and-6)-CFSE/FAM (150 μg; C-1311; Invitrogen, Carlsbad, CA, USA) was added to 1 mg sOX40 for 90 min at 4°C.

    High Performance Liquid Chromatography:

    Article Title: Inhibition of osteoblast mineralization by phosphorylated phage-derived apatite-specific peptide
    Article Snippet: Peptides were synthesized using Fmoc solid-phase chemistry according to standard peptide synthesis procedures and characterized as having > 90 % purity by high-performance liquid chromatography (University of Michigan Proteomics & Peptide Synthesis Core). .. For experiments involving fluorescence, peptides were labelled on resin before cleavage using 5-(and-6)-carboxyfluorescein succinimidyl ester (Molecular Probes C-1311).

    Article Title: Phosphorylation-dependent mineral type specificity for apatite-binding peptide sequences
    Article Snippet: All peptides were fabricated on a Rainin Symphony Synthesizer by Fmoc solid-phase chemistry according to standard peptide synthesis procedures and characterized as having > 86% purity by high-performance liquid chromatography (University of Michigan Protein Core). .. For experiments involving fluorescence, peptides were labelled on resin before cleavage using 5-(and-6)-carboxyfluorescein succinimidyl ester (Molecular Probes C-1311) (University of Michigan Protein Core).

    Conjugation Assay:

    Article Title: Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain
    Article Snippet: .. Tyramide synthesis To synthesize tyramide reagents [ ], the following succinimidyl esters were used for conjugation with tyramine (Sigma-Aldrich: St. Louis, MO, USA T2879): 5-(and-6)-carboxyfluorescein succinimidyl ester (FAM-SE; Molecular Probes C-1311), 5-(and-6)-carboxytetramethylrhodamine succinimidyl ester (TAMRA-SE; Molecular Probes C-1171) and DyLight 633 N-hydroxysuccinimide ester (DyLight633-SE; Pierce: Rockford, IL, USA 46414). .. Each succinimidyl ester was dissolved at a concentration of 10 mg/ml in dimethylformamide (Sigma-Aldrich D4551).

    Flow Cytometry:

    Article Title: Technical Advance: Soluble OX40 molecule mimics regulatory T cell modulatory activity on Fc?RI-dependent mast cell degranulation
    Article Snippet: DMSO-resuspended 5-(and-6)-CFSE/FAM (150 μg; C-1311; Invitrogen, Carlsbad, CA, USA) was added to 1 mg sOX40 for 90 min at 4°C. .. WT or Tnfsf4 −/− BMMCs were incubated with sOX40-CFSE (20 μg/ml) for 30 min at room temperature, washed, and analyzed by flow cytometry.

    Article Title: Antibodies to the Iron Uptake ABC Transporter Lipoproteins PiaA and PiuA Promote Opsonophagocytosis of Streptococcus pneumoniae
    Article Snippet: To assess the effect of anti-PiuA and anti-PiaA antibodies on the interaction of S. pneumoniae with phagocytes, we measured the proportion of a neutrophil cell line associated with fluorescent bacteria by using a previously described flow cytometry opsonophagocytosis assay ( , , ). .. S. pneumoniae strains were fluorescently labeled by incubation with 5,6-carboxyfluorescein-succinimidyl ester (FAM-SE; Molecular Probes, Eugene, Oreg.) solution (10 mg/ml in dimethyl sulfoxide; Sigma) in 0.1 M sodium bicarbonate buffer for 1 h at 37°C and then washed six times with Hanks balanced salt solution (HBSS) in 0.2% bovine serum albumin and stored in aliquots at −70°C in 10% glycerol (approximately 109 CFU/ml).

    Article Title: Serum Amyloid P Aids Complement-Mediated Immunity to Streptococcus pneumoniae
    Article Snippet: Phagocytosis of S. pneumoniae in serum from Apcs−/− , Apcs−/− .C1qa−/− , and wild-type mice was investigated using a previously described flow cytometry assay and the human tissue culture cell line HL-60 (promyelocytic leukemia cells; CCL240; American Type Culture Collection, http://www.atcc.org/ ) differentiated into granulocytes [ , ]. .. S. pneumoniae were fluorescently labelled with 5,6-carboxyfluorescein succinimidyl ester (FAM-SE; Molecular Probes, http://probes.invitrogen.com/ ) as described and stored at −70 °C in 10% glycerol as single-use aliquots (7 × 108 cfu/ml).

    Biomarker Assay:

    Article Title: Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1 2
    Article Snippet: Paragraph title: Development of a Predictive Biomarker Model of C1311 Sensitivity ... To derive a gene expression signature of C1311 sensitivity, we began by selecting candidate sensitivity biomarkers by rank-based correlation ( r s = 0.4) of C1311 IC50 values across the NCI-60 panel, finding that 219/22283 Affymetrix HG-U133A microarray probes meet this criterion (false discovery rate = 0.1 by random permutation testing, for probe information and correlation coefficients, see ).

    Adoptive Transfer Assay:

    Article Title: Eradication of Cryptosporidium parvum Infection by Mice with Ovalbumin-Specific T Cells
    Article Snippet: CFSE (catalog no. C-1311; Molecular Probes, Eugene, Oreg.), stored as a 10 mM stock solution in dimethyl sulfoxide, was diluted to 4 μM in 10 ml of saline at 37°C, and 4 × 107 lymphocytes were added in 10 ml of saline. .. Mice were injected with 1 mg of BUdR i.p. 36 h after adoptive transfer of T cells and euthanized 12 h later.

    Sequencing:

    Article Title: Phosphorylation-dependent mineral type specificity for apatite-binding peptide sequences
    Article Snippet: Phosphorylations at specific serine residues in the peptide sequence were achieved using preformed, protected phosphoserine amino acids. .. For experiments involving fluorescence, peptides were labelled on resin before cleavage using 5-(and-6)-carboxyfluorescein succinimidyl ester (Molecular Probes C-1311) (University of Michigan Protein Core).

    Injection:

    Article Title: Marrow-Derived CD40-Positive Cells Are Required for Mice To Clear Cryptosporidium parvum Infection
    Article Snippet: These cells were fluorescence labeled by CFSE (catalog no. C-1311; Molecular Probes, Portland, Oreg.) as previously described ( ) and resuspended at 108 /ml in HBSS. .. Ten million cells were injected intraperitoneally into wild-type and knockout recipients as indicated below.

    Article Title: Eradication of Cryptosporidium parvum Infection by Mice with Ovalbumin-Specific T Cells
    Article Snippet: CFSE (catalog no. C-1311; Molecular Probes, Eugene, Oreg.), stored as a 10 mM stock solution in dimethyl sulfoxide, was diluted to 4 μM in 10 ml of saline at 37°C, and 4 × 107 lymphocytes were added in 10 ml of saline. .. After 10 min at 37°C, the cells were pelleted by centrifugation, resuspended in sterile HBSS, and injected i.p.

    Binding Assay:

    Article Title: Technical Advance: Soluble OX40 molecule mimics regulatory T cell modulatory activity on Fc?RI-dependent mast cell degranulation
    Article Snippet: Paragraph title: sOX40 fluorescein labeling, binding, and competition assay ... DMSO-resuspended 5-(and-6)-CFSE/FAM (150 μg; C-1311; Invitrogen, Carlsbad, CA, USA) was added to 1 mg sOX40 for 90 min at 4°C.

    Molecular Weight:

    Article Title: Early Events in Phagosome Establishment Are Required for Intracellular Survival of Legionella pneumophila
    Article Snippet: .. 5,6-Carboxyfluorescein succinimidyl ester (FSE) (catalog no. C-1311) and tetramethylrhodamine dextran (Rh-dextran) (70,000 molecular weight [MW], lysine fixable) (catalog no. D-1818) were purchased from Molecular Probes (Seattle, Wash.). .. Also purchased were RPMI 1640 medium lacking l -glutamine (RPMI) (JRH Biosciences, Lenexa, Kans.), l -glutamine (Gln) (Mediatech, Washington, D.C.), and agarose (IBI, New Haven, Conn.).

    In Vivo:

    Article Title: Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1 2
    Article Snippet: Taken together, the promising in vitro and in vivo results for C1311 suggest the possibility of testing it in a future clinical trial, a setting where significant efficiencies may achieved by biomarker-guarded selection of patients most likely to respond to therapy [ ]. .. To derive a gene expression signature of C1311 sensitivity, we began by selecting candidate sensitivity biomarkers by rank-based correlation ( r s = 0.4) of C1311 IC50 values across the NCI-60 panel, finding that 219/22283 Affymetrix HG-U133A microarray probes meet this criterion (false discovery rate = 0.1 by random permutation testing, for probe information and correlation coefficients, see ).

    Fluorescence:

    Article Title: Inhibition of osteoblast mineralization by phosphorylated phage-derived apatite-specific peptide
    Article Snippet: .. For experiments involving fluorescence, peptides were labelled on resin before cleavage using 5-(and-6)-carboxyfluorescein succinimidyl ester (Molecular Probes C-1311). ..

    Article Title: Tunable Thermal-Sensitive Polymer-Graphene Oxide Composite for Efficient Capture and Release of Viable Circulating Tumor Cells
    Article Snippet: To show the surface availability of the amine groups from the GO-PEG in polymer-GO composite films, the drop-cast films were incubated with 0.25mM aqueous solution of an amine reactive dye, FSE (5-(and-6)-carboxyfluorescein, succinimidyl ester; Life Technologies (C1311)) for 30 minutes at 40°C and then washed with copious amount of DI water. .. To show the surface availability of the amine groups from the GO-PEG in polymer-GO composite films, the drop-cast films were incubated with 0.25mM aqueous solution of an amine reactive dye, FSE (5-(and-6)-carboxyfluorescein, succinimidyl ester; Life Technologies (C1311)) for 30 minutes at 40°C and then washed with copious amount of DI water.

    Article Title: Phosphorylation-dependent mineral type specificity for apatite-binding peptide sequences
    Article Snippet: .. For experiments involving fluorescence, peptides were labelled on resin before cleavage using 5-(and-6)-carboxyfluorescein succinimidyl ester (Molecular Probes C-1311) (University of Michigan Protein Core). ..

    Article Title: Marrow-Derived CD40-Positive Cells Are Required for Mice To Clear Cryptosporidium parvum Infection
    Article Snippet: .. These cells were fluorescence labeled by CFSE (catalog no. C-1311; Molecular Probes, Portland, Oreg.) as previously described ( ) and resuspended at 108 /ml in HBSS. .. Ten million cells were injected intraperitoneally into wild-type and knockout recipients as indicated below.

    Avidin-Biotin Assay:

    Article Title: Tunable Thermal-Sensitive Polymer-Graphene Oxide Composite for Efficient Capture and Release of Viable Circulating Tumor Cells
    Article Snippet: In the second step, the device was functionalized by immobilizing anti-EpCAM on the surface available GO through a cross-linker (N-γ-maleimidobutyryl-oxysuccinimide ester, sulfo-GMBS) and avidin-biotin mediated bio-conjugation, providing cell capture/release functionality ( ). .. To show the surface availability of the amine groups from the GO-PEG in polymer-GO composite films, the drop-cast films were incubated with 0.25mM aqueous solution of an amine reactive dye, FSE (5-(and-6)-carboxyfluorescein, succinimidyl ester; Life Technologies (C1311)) for 30 minutes at 40°C and then washed with copious amount of DI water.

    Labeling:

    Article Title: Technical Advance: Soluble OX40 molecule mimics regulatory T cell modulatory activity on Fc?RI-dependent mast cell degranulation
    Article Snippet: Paragraph title: sOX40 fluorescein labeling, binding, and competition assay ... DMSO-resuspended 5-(and-6)-CFSE/FAM (150 μg; C-1311; Invitrogen, Carlsbad, CA, USA) was added to 1 mg sOX40 for 90 min at 4°C.

    Article Title: Antibodies to the Iron Uptake ABC Transporter Lipoproteins PiaA and PiuA Promote Opsonophagocytosis of Streptococcus pneumoniae
    Article Snippet: .. S. pneumoniae strains were fluorescently labeled by incubation with 5,6-carboxyfluorescein-succinimidyl ester (FAM-SE; Molecular Probes, Eugene, Oreg.) solution (10 mg/ml in dimethyl sulfoxide; Sigma) in 0.1 M sodium bicarbonate buffer for 1 h at 37°C and then washed six times with Hanks balanced salt solution (HBSS) in 0.2% bovine serum albumin and stored in aliquots at −70°C in 10% glycerol (approximately 109 CFU/ml). .. The human cell line HL-60 (promyelocytic leukemia cells; CCL240; American Type Culture Collection, Manassas, Va.) was used to provide the effector cells after differentiation into granulocytes by using previously described protocols ( ).

    Mouse Assay:

    Article Title: Serum Amyloid P Aids Complement-Mediated Immunity to Streptococcus pneumoniae
    Article Snippet: Phagocytosis of S. pneumoniae in serum from Apcs−/− , Apcs−/− .C1qa−/− , and wild-type mice was investigated using a previously described flow cytometry assay and the human tissue culture cell line HL-60 (promyelocytic leukemia cells; CCL240; American Type Culture Collection, http://www.atcc.org/ ) differentiated into granulocytes [ , ]. .. S. pneumoniae were fluorescently labelled with 5,6-carboxyfluorescein succinimidyl ester (FAM-SE; Molecular Probes, http://probes.invitrogen.com/ ) as described and stored at −70 °C in 10% glycerol as single-use aliquots (7 × 108 cfu/ml).

    Article Title: Eradication of Cryptosporidium parvum Infection by Mice with Ovalbumin-Specific T Cells
    Article Snippet: CFSE (catalog no. C-1311; Molecular Probes, Eugene, Oreg.), stored as a 10 mM stock solution in dimethyl sulfoxide, was diluted to 4 μM in 10 ml of saline at 37°C, and 4 × 107 lymphocytes were added in 10 ml of saline. .. Mice were injected with 1 mg of BUdR i.p. 36 h after adoptive transfer of T cells and euthanized 12 h later.

    Microscopy:

    Article Title: Tunable Thermal-Sensitive Polymer-Graphene Oxide Composite for Efficient Capture and Release of Viable Circulating Tumor Cells
    Article Snippet: To show the surface availability of the amine groups from the GO-PEG in polymer-GO composite films, the drop-cast films were incubated with 0.25mM aqueous solution of an amine reactive dye, FSE (5-(and-6)-carboxyfluorescein, succinimidyl ester; Life Technologies (C1311)) for 30 minutes at 40°C and then washed with copious amount of DI water. .. To show the surface availability of the amine groups from the GO-PEG in polymer-GO composite films, the drop-cast films were incubated with 0.25mM aqueous solution of an amine reactive dye, FSE (5-(and-6)-carboxyfluorescein, succinimidyl ester; Life Technologies (C1311)) for 30 minutes at 40°C and then washed with copious amount of DI water.

    Article Title: Marrow-Derived CD40-Positive Cells Are Required for Mice To Clear Cryptosporidium parvum Infection
    Article Snippet: These cells were fluorescence labeled by CFSE (catalog no. C-1311; Molecular Probes, Portland, Oreg.) as previously described ( ) and resuspended at 108 /ml in HBSS. .. The division of these cells was assessed by fluorescence-activated cell sorter analysis, and their appearance in the gut was detected by fluorescence microscopy of frozen sections.

    Article Title: L-arginine-NO-cGMP signalling pathway in pancreatitis
    Article Snippet: Paragraph title: Immunofluorescent microscopy ... FITC-tyramine conjugate was synthesised by us from tyramine-HCl (Sigma) and FITC-succinimidyl ester (FITC-NHS, #C1311 from Molecular Probes) in DMF.

    Functional Assay:

    Article Title: Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1 2
    Article Snippet: To derive a gene expression signature of C1311 sensitivity, we began by selecting candidate sensitivity biomarkers by rank-based correlation ( r s = 0.4) of C1311 IC50 values across the NCI-60 panel, finding that 219/22283 Affymetrix HG-U133A microarray probes meet this criterion (false discovery rate = 0.1 by random permutation testing, for probe information and correlation coefficients, see ). .. Evaluation of the potential functional associations of these 219 probe sets using the Ingenuity Pathway Analysis program identified the glycerophospholipid metabolism pathway as the most significantly enriched, supporting in cancer cells our observations associating C1311 with lipid biogenesis in budding yeast.

    Selection:

    Article Title: Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1 2
    Article Snippet: Taken together, the promising in vitro and in vivo results for C1311 suggest the possibility of testing it in a future clinical trial, a setting where significant efficiencies may achieved by biomarker-guarded selection of patients most likely to respond to therapy [ ]. .. To derive a gene expression signature of C1311 sensitivity, we began by selecting candidate sensitivity biomarkers by rank-based correlation ( r s = 0.4) of C1311 IC50 values across the NCI-60 panel, finding that 219/22283 Affymetrix HG-U133A microarray probes meet this criterion (false discovery rate = 0.1 by random permutation testing, for probe information and correlation coefficients, see ).

    Agarose Gel Electrophoresis:

    Article Title: Identification of ANXA2 (annexin A2) as a specific bleomycin target to induce pulmonary fibrosis by impeding TFEB-mediated autophagic flux
    Article Snippet: Briefly, 2.1 mM 5-(and-6)-carboxyfluorescein, succinimidyl ester (5(6)-FAM, SE (Thermo Fisher Scientific, C1311) was incubated with 0.6 mM bleomycin A5 in 0.2 M NaHCO3 , pH 8.3, at 4ºC overnight. .. The products were then separated on a 1% agarose gel with 40 mM MES buffer, pH 6.5.

    In Vitro:

    Article Title: Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1Use of Yeast Chemigenomics and COXEN Informatics in Preclinical Evaluation of Anticancer Agents 1 2
    Article Snippet: Taken together, the promising in vitro and in vivo results for C1311 suggest the possibility of testing it in a future clinical trial, a setting where significant efficiencies may achieved by biomarker-guarded selection of patients most likely to respond to therapy [ ]. .. To derive a gene expression signature of C1311 sensitivity, we began by selecting candidate sensitivity biomarkers by rank-based correlation ( r s = 0.4) of C1311 IC50 values across the NCI-60 panel, finding that 219/22283 Affymetrix HG-U133A microarray probes meet this criterion (false discovery rate = 0.1 by random permutation testing, for probe information and correlation coefficients, see ).

    Opsonophagocytosis Assay:

    Article Title: Antibodies to the Iron Uptake ABC Transporter Lipoproteins PiaA and PiuA Promote Opsonophagocytosis of Streptococcus pneumoniae
    Article Snippet: To assess the effect of anti-PiuA and anti-PiaA antibodies on the interaction of S. pneumoniae with phagocytes, we measured the proportion of a neutrophil cell line associated with fluorescent bacteria by using a previously described flow cytometry opsonophagocytosis assay ( , , ). .. S. pneumoniae strains were fluorescently labeled by incubation with 5,6-carboxyfluorescein-succinimidyl ester (FAM-SE; Molecular Probes, Eugene, Oreg.) solution (10 mg/ml in dimethyl sulfoxide; Sigma) in 0.1 M sodium bicarbonate buffer for 1 h at 37°C and then washed six times with Hanks balanced salt solution (HBSS) in 0.2% bovine serum albumin and stored in aliquots at −70°C in 10% glycerol (approximately 109 CFU/ml).

    Knock-Out:

    Article Title: Marrow-Derived CD40-Positive Cells Are Required for Mice To Clear Cryptosporidium parvum Infection
    Article Snippet: These cells were fluorescence labeled by CFSE (catalog no. C-1311; Molecular Probes, Portland, Oreg.) as previously described ( ) and resuspended at 108 /ml in HBSS. .. Ten million cells were injected intraperitoneally into wild-type and knockout recipients as indicated below.

    Concentration Assay:

    Article Title: Mechanism of polarization of Listeria monocytogenes surface protein ActA
    Article Snippet: The surface of bacteria was labelled with either FAM-SE (Molecular Probes C1311; Eugene, OR) or Marina Blue-SE (Molecular Probes M10165; Eugene OR). .. Aliquots of dye were resuspended in DMSO to 10 mg ml−1 and dye was immediately added to resuspended bacteria to a concentration of 200 µg ml−1 .

    Article Title: Multicolor fluorescent in situ hybridization to define abutting and overlapping gene expression in the embryonic zebrafish brain
    Article Snippet: Tyramide synthesis To synthesize tyramide reagents [ ], the following succinimidyl esters were used for conjugation with tyramine (Sigma-Aldrich: St. Louis, MO, USA T2879): 5-(and-6)-carboxyfluorescein succinimidyl ester (FAM-SE; Molecular Probes C-1311), 5-(and-6)-carboxytetramethylrhodamine succinimidyl ester (TAMRA-SE; Molecular Probes C-1171) and DyLight 633 N-hydroxysuccinimide ester (DyLight633-SE; Pierce: Rockford, IL, USA 46414). .. Each succinimidyl ester was dissolved at a concentration of 10 mg/ml in dimethylformamide (Sigma-Aldrich D4551).

    Article Title: L-arginine-NO-cGMP signalling pathway in pancreatitis
    Article Snippet: FITC-tyramine conjugate was synthesised by us from tyramine-HCl (Sigma) and FITC-succinimidyl ester (FITC-NHS, #C1311 from Molecular Probes) in DMF. .. To visualize NOS simultaneously with nitrotyrosine (marker of oxidative damage to proteins), sections were immunoreacted with anti-NOS Abs in the presence of mouse monoclonal anti-nitrotyrosine Abs (Zytomed GmbH, Berlin, Germany, applied at a final concentration of 5 μg/ml).

    Marker:

    Article Title: Serum Amyloid P Aids Complement-Mediated Immunity to Streptococcus pneumoniae
    Article Snippet: S. pneumoniae were fluorescently labelled with 5,6-carboxyfluorescein succinimidyl ester (FAM-SE; Molecular Probes, http://probes.invitrogen.com/ ) as described and stored at −70 °C in 10% glycerol as single-use aliquots (7 × 108 cfu/ml). .. HL-60 cells (105 ) were added to each well and incubated for 30 min at 37 °C, fixed with 3% PFA, and analysed using a FACScalibur flow cytometer (minimum of 6,000 cells per sample) to identify the proportion of cells associated with fluorescent bacteria as a marker of phagocytosis [ ].

    Article Title: L-arginine-NO-cGMP signalling pathway in pancreatitis
    Article Snippet: FITC-tyramine conjugate was synthesised by us from tyramine-HCl (Sigma) and FITC-succinimidyl ester (FITC-NHS, #C1311 from Molecular Probes) in DMF. .. Bound goat anti-8OHG Ab (marker of oxidative damage to proteins) was visualized with FITC-conjugated donkey-anti-goat AB (Dianova, Hamburg, Germany).

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