6 fam labeled dna oligos  (Integrated DNA Technologies)


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    Name:
    DNA oligo
    Description:
    Single stranded pooled or duplexed DNA synthesized to customer specifications Sspecialized platforms with industry leading synthesis capabilities deliver the purest primers for PCR dual labelled probes for qPCR indexed adapters and fusion primers for sequencing and a variety of advanced and custom products
    Catalog Number:
    do-577595
    Price:
    None
    Category:
    Nucleic acids
    Buy from Supplier


    Structured Review

    Integrated DNA Technologies 6 fam labeled dna oligos
    Single stranded pooled or duplexed DNA synthesized to customer specifications Sspecialized platforms with industry leading synthesis capabilities deliver the purest primers for PCR dual labelled probes for qPCR indexed adapters and fusion primers for sequencing and a variety of advanced and custom products
    https://www.bioz.com/result/6 fam labeled dna oligos/product/Integrated DNA Technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    6 fam labeled dna oligos - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Mechanism of HIV-1 RNA Dimerization in the Central Region of the Genome and Significance for Viral Evolution *
    Article Snippet: .. DNA oligonucleotides and the HPLC-purified RNA strand used for CD spectra analyses were purchased from Integrated DNA Technologies, Inc. (Coralville, IA). ..

    Multiplex Assay:

    Article Title: A Lysate Proteome Engineering Strategy for Enhancing Cell-Free Metabolite Production
    Article Snippet: .. 2.1 Generation and Validation of Genome Engineered Strains using MAGE All multiplex allele-specific PCR (MASC-PCR), Sanger Sequencing oligos, and recombineering oligos were created manually and ordered from IDT (Coralville, IA) with standard purification. ..

    IA:

    Article Title: Mechanism of HIV-1 RNA Dimerization in the Central Region of the Genome and Significance for Viral Evolution *
    Article Snippet: .. DNA oligonucleotides and the HPLC-purified RNA strand used for CD spectra analyses were purchased from Integrated DNA Technologies, Inc. (Coralville, IA). ..

    Article Title: Solid-phase enzyme catalysis of DNA end repair and 3′ A-tailing reduces GC-bias in next-generation sequencing of human genomic DNA
    Article Snippet: .. DNA oligos were synthesized by Integrated DNA Technologies (Coralville, IA) and dissolved in nuclease-free water prior to use (Supplementary Table ). ..

    Article Title: RNA Polymerase II Trigger Loop Mobility
    Article Snippet: .. RNA and DNA oligomers were purchased from Integrated DNA Technologies (Coralville, IA). .. RNA oligonucleotides (16.7 pmol) were labeled with [γ-32 P]ATP (16.7 pmol) (3000 Ci/mmol, PerkinElmer Life Sciences catalog no. BLU002A) using T4 polynucleotide kinase (Promega catalog no. M4101) in transcription buffer TB(40) (20 m m Tris-HCl (pH 7.9), 40 m m KCl, 5 m m MgCl2 , 2 m m 2-mercaptoethanol).

    Synthesized:

    Article Title: Solid-phase enzyme catalysis of DNA end repair and 3′ A-tailing reduces GC-bias in next-generation sequencing of human genomic DNA
    Article Snippet: .. DNA oligos were synthesized by Integrated DNA Technologies (Coralville, IA) and dissolved in nuclease-free water prior to use (Supplementary Table ). ..

    Purification:

    Article Title: A Lysate Proteome Engineering Strategy for Enhancing Cell-Free Metabolite Production
    Article Snippet: .. 2.1 Generation and Validation of Genome Engineered Strains using MAGE All multiplex allele-specific PCR (MASC-PCR), Sanger Sequencing oligos, and recombineering oligos were created manually and ordered from IDT (Coralville, IA) with standard purification. ..

    Polymerase Chain Reaction:

    Article Title: A Lysate Proteome Engineering Strategy for Enhancing Cell-Free Metabolite Production
    Article Snippet: .. 2.1 Generation and Validation of Genome Engineered Strains using MAGE All multiplex allele-specific PCR (MASC-PCR), Sanger Sequencing oligos, and recombineering oligos were created manually and ordered from IDT (Coralville, IA) with standard purification. ..

    other:

    Article Title: Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
    Article Snippet: DNA oligonucleotides and tracrRNA were purchased from Integrated DNA Technologies (IDT).

    Article Title: CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks
    Article Snippet: Additional files Sequences of plasmids, DNA oligonucleotides, and RNA oligonucleotides used in this work.

    Article Title: RanDeL-seq: A high-throughput method to map viral cis- and trans-acting elements
    Article Snippet: DNA oligonucleotides and synthetic dsDNA were obtained from Integrated DNA Technologies (Coralville, IA, USA).

    Sequencing:

    Article Title: A Lysate Proteome Engineering Strategy for Enhancing Cell-Free Metabolite Production
    Article Snippet: .. 2.1 Generation and Validation of Genome Engineered Strains using MAGE All multiplex allele-specific PCR (MASC-PCR), Sanger Sequencing oligos, and recombineering oligos were created manually and ordered from IDT (Coralville, IA) with standard purification. ..

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  • 99
    Integrated DNA Technologies 6 fam labeled dna oligos
    6 Fam Labeled Dna Oligos, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6 fam labeled dna oligos/product/Integrated DNA Technologies
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    6 fam labeled dna oligos - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    85
    Integrated DNA Technologies 6 fam labeled oligos
    Strategy. A: The positions of the primers ( arrows ) and probe on the wild-type and mutant CLN3 gene are shown. The fluorophore on the probe is indicated with an asterisk ; not drawn to scale. B: The sequence of the probe is shown in between the complementary sequences of the wild-type and deletion alleles, with vertical lines connecting the base-paired residues. The –F on the 3′ end of the probe indicates the <t>6-FAM</t> fluorophore and shows its position with respect to the G residues on the opposite strand. The probe is fully base-paired with the mutant sequence, but has three unmatched nucleotides at the 5′ end when annealed to a wild-type amplicon. The G residues that contribute to the quenching of the fluorescent signal are underlined in the normal and mutant sequences.
    6 Fam Labeled Oligos, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6 fam labeled oligos/product/Integrated DNA Technologies
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    6 fam labeled oligos - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    93
    Integrated DNA Technologies 6 carboxyfluorescein fam labeled primers
    Strategy. A: The positions of the primers ( arrows ) and probe on the wild-type and mutant CLN3 gene are shown. The fluorophore on the probe is indicated with an asterisk ; not drawn to scale. B: The sequence of the probe is shown in between the complementary sequences of the wild-type and deletion alleles, with vertical lines connecting the base-paired residues. The –F on the 3′ end of the probe indicates the <t>6-FAM</t> fluorophore and shows its position with respect to the G residues on the opposite strand. The probe is fully base-paired with the mutant sequence, but has three unmatched nucleotides at the 5′ end when annealed to a wild-type amplicon. The G residues that contribute to the quenching of the fluorescent signal are underlined in the normal and mutant sequences.
    6 Carboxyfluorescein Fam Labeled Primers, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6 carboxyfluorescein fam labeled primers/product/Integrated DNA Technologies
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    6 carboxyfluorescein fam labeled primers - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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    Strategy. A: The positions of the primers ( arrows ) and probe on the wild-type and mutant CLN3 gene are shown. The fluorophore on the probe is indicated with an asterisk ; not drawn to scale. B: The sequence of the probe is shown in between the complementary sequences of the wild-type and deletion alleles, with vertical lines connecting the base-paired residues. The –F on the 3′ end of the probe indicates the 6-FAM fluorophore and shows its position with respect to the G residues on the opposite strand. The probe is fully base-paired with the mutant sequence, but has three unmatched nucleotides at the 5′ end when annealed to a wild-type amplicon. The G residues that contribute to the quenching of the fluorescent signal are underlined in the normal and mutant sequences.

    Journal: The Journal of molecular diagnostics : JMD

    Article Title: Homogeneous Polymerase Chain Reaction Nucleobase Quenching Assay to Detect the 1-kbp Deletion in CLN3 That Causes Batten Disease

    doi:

    Figure Lengend Snippet: Strategy. A: The positions of the primers ( arrows ) and probe on the wild-type and mutant CLN3 gene are shown. The fluorophore on the probe is indicated with an asterisk ; not drawn to scale. B: The sequence of the probe is shown in between the complementary sequences of the wild-type and deletion alleles, with vertical lines connecting the base-paired residues. The –F on the 3′ end of the probe indicates the 6-FAM fluorophore and shows its position with respect to the G residues on the opposite strand. The probe is fully base-paired with the mutant sequence, but has three unmatched nucleotides at the 5′ end when annealed to a wild-type amplicon. The G residues that contribute to the quenching of the fluorescent signal are underlined in the normal and mutant sequences.

    Article Snippet: A single probe with only a single fluorescent label is less expensive than two dual-labeled probes used for 5′ nuclease assays, and also less expensive than the two labeled probes used for fluorescence resonance energy transfer assays., In contrast to Crockett and Wittwer we used 6-FAM (6-carboxyfluorescein) instead of fluorescein (fluorescein isothiocyanate) because the excitation and emission wavelengths are very similar, the 6-FAM fluorescence is quenched by G residues, and 6-FAM-labeled oligos are less expensive because they do not require additional purification before use (Integrated DNA Technologies, personal communication).

    Techniques: Mutagenesis, Sequencing, Amplification