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Journal: bioRxiv
Article Title: Viral Insulin/IGF-like Peptides Selectively Activate Host Insulin/IGF Signaling pathways during Grouper Iridovirus Infection
doi: 10.1101/2024.06.13.598936
Figure Lengend Snippet: A- Immunofluorescence of GK cells transfected with either pcDNA3.1+-eGFP plasmid or pcDNA3.1+-GIV-VILP-eGFP. pcDNA3.1+-GIV-VILP-eGFP expressing cells with GIV-VILP-eGFP in green colocalize with Golgi apparatus highlighted by 58K-Golgi antibodies in red, nucleus was stained with Hoechst (blue). B- Western blot analysis of GIV-VILP-eGFP proteins levels after 6h treatment with Brefeldin A (10µg/mL). (C) Viral transcripts GIV90, GIV87, GIV39, and GIV32 (GIV-VILP) were quantified by RT-qPCR after RNA extraction and cDNA synthesis from GK infected cells (MOI 1) at different hours post infection (hpi). Data are expressed as means ± SDs (n = 3). B-Actin is used for normalization. MOI:1. (D) Scheme of the mass spectrometry analysis of virions’ structural proteins, refer to . (E) Scheme of the mass spectrometry analysis of secreted proteins from the infection of GK cells by GIV virus, refer to .
Article Snippet: After the blocking step,
Techniques: Immunofluorescence, Transfection, Plasmid Preparation, Expressing, Staining, Western Blot, Quantitative RT-PCR, RNA Extraction, cDNA Synthesis, Infection, Mass Spectrometry, Virus
Journal: Cell reports
Article Title: NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation
doi: 10.1016/j.celrep.2024.114070
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Recombinant, Control, Cytotoxicity Assay, Enzyme-linked Immunosorbent Assay, Reverse Transcription, SYBR Green Assay, Western Blot, Expressing, Mutagenesis, Transgenic Assay, Plasmid Preparation, Construct, Software, Microscopy