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Santa Cruz Biotechnology syntaxin 1a
Syntaxin 1a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology antimouse syntaxin 1 antibody
Primer sequences for real-time reverse transcription polymerase chain reaction.
Antimouse Syntaxin 1 Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation"

Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

Journal: Molecular Vision

doi:

Primer sequences for real-time reverse transcription polymerase chain reaction.
Figure Legend Snippet: Primer sequences for real-time reverse transcription polymerase chain reaction.

Techniques Used: Sequencing

Primary mouse retinal ganglion cells isolated with two-step immunopanning. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.
Figure Legend Snippet: Primary mouse retinal ganglion cells isolated with two-step immunopanning. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

Techniques Used: Isolation, Labeling, Fluorescence, Staining, Construct

Primary mouse retinal ganglion cells isolated by direct magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.
Figure Legend Snippet: Primary mouse retinal ganglion cells isolated by direct magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

Techniques Used: Isolation, Labeling, Fluorescence, Staining, Construct

Primary mouse retinal ganglion cells isolated by immunopanning-magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.
Figure Legend Snippet: Primary mouse retinal ganglion cells isolated by immunopanning-magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

Techniques Used: Isolation, Labeling, Fluorescence, Staining, Construct

Representative western immunoblots for glial fibrillary acidic protein, syntaxin 1, and β-actin. Primary mouse retinal ganglion cells were isolated using three different systems, including two-step immunopanning, direct magnetic separation, and immunopanning-magnetic separation.
Figure Legend Snippet: Representative western immunoblots for glial fibrillary acidic protein, syntaxin 1, and β-actin. Primary mouse retinal ganglion cells were isolated using three different systems, including two-step immunopanning, direct magnetic separation, and immunopanning-magnetic separation.

Techniques Used: Western Blot, Isolation

Quantitative data of real-time reverse transcription polymerase chain reaction (RT-PCR) for glial fibrillary acidic protein (GFAP) and syntaxin 1. A relative RNA ratio was calculated by dividing the value of each purification method by the value of whole retinal cell suspension. Data were expressed as the mean±SEM. (n=9 for each method). The p value for GFAP and syntaxin 1 was 0.021* and 0.022**, respectively.
Figure Legend Snippet: Quantitative data of real-time reverse transcription polymerase chain reaction (RT-PCR) for glial fibrillary acidic protein (GFAP) and syntaxin 1. A relative RNA ratio was calculated by dividing the value of each purification method by the value of whole retinal cell suspension. Data were expressed as the mean±SEM. (n=9 for each method). The p value for GFAP and syntaxin 1 was 0.021* and 0.022**, respectively.

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Purification


Structured Review

Santa Cruz Biotechnology syntaxin 1
Primer sequences for real-time reverse transcription polymerase chain reaction.
Syntaxin 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/syntaxin 1/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
syntaxin 1 - by Bioz Stars, 2024-07
93/100 stars

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1) Product Images from "Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation"

Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

Journal: Molecular Vision

doi:

Primer sequences for real-time reverse transcription polymerase chain reaction.
Figure Legend Snippet: Primer sequences for real-time reverse transcription polymerase chain reaction.

Techniques Used: Sequencing

Primary mouse retinal ganglion cells isolated with two-step immunopanning. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.
Figure Legend Snippet: Primary mouse retinal ganglion cells isolated with two-step immunopanning. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

Techniques Used: Isolation, Labeling, Fluorescence, Staining, Construct

Primary mouse retinal ganglion cells isolated by direct magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.
Figure Legend Snippet: Primary mouse retinal ganglion cells isolated by direct magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

Techniques Used: Isolation, Labeling, Fluorescence, Staining, Construct

Primary mouse retinal ganglion cells isolated by immunopanning-magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.
Figure Legend Snippet: Primary mouse retinal ganglion cells isolated by immunopanning-magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

Techniques Used: Isolation, Labeling, Fluorescence, Staining, Construct

Representative western immunoblots for glial fibrillary acidic protein, syntaxin 1, and β-actin. Primary mouse retinal ganglion cells were isolated using three different systems, including two-step immunopanning, direct magnetic separation, and immunopanning-magnetic separation.
Figure Legend Snippet: Representative western immunoblots for glial fibrillary acidic protein, syntaxin 1, and β-actin. Primary mouse retinal ganglion cells were isolated using three different systems, including two-step immunopanning, direct magnetic separation, and immunopanning-magnetic separation.

Techniques Used: Western Blot, Isolation

Quantitative data of real-time reverse transcription polymerase chain reaction (RT-PCR) for glial fibrillary acidic protein (GFAP) and syntaxin 1. A relative RNA ratio was calculated by dividing the value of each purification method by the value of whole retinal cell suspension. Data were expressed as the mean±SEM. (n=9 for each method). The p value for GFAP and syntaxin 1 was 0.021* and 0.022**, respectively.
Figure Legend Snippet: Quantitative data of real-time reverse transcription polymerase chain reaction (RT-PCR) for glial fibrillary acidic protein (GFAP) and syntaxin 1. A relative RNA ratio was calculated by dividing the value of each purification method by the value of whole retinal cell suspension. Data were expressed as the mean±SEM. (n=9 for each method). The p value for GFAP and syntaxin 1 was 0.021* and 0.022**, respectively.

Techniques Used: Reverse Transcription Polymerase Chain Reaction, Purification


Structured Review

Santa Cruz Biotechnology syntaxin
The different retinal cell types are produced normally in the PAX77 +/+ eye. Horizontal sections through wild-type and PAX77 +/+ eyes. (A) Expression of the RGC marker Brn3a at E18.5. (B) BrdU labelling (brown) of retinal progenitors in S-phase in E18.5 eyes. Sections are counterstained with cresyl violet. Expression of (C, J) Pax6 (green) and GFAP (red), <t>(D)</t> <t>Islet1,</t> (E) Chx10, (F) <t>Syntaxin,</t> (G) R-cadherin, (H) N-cadherin and (I) Brn3a in the retinae of wild-type and PAX77 +/+ pups at P6 (C to I) and P14 (J). (C,F) Sections are counterstained with TOPRO3. Arrowheads in C,D,F: amacrine cells; arrows in C,G: horizontal cells; arrows in E: bipolar and progenitor cells; arrowhead in G: outer plexiform layer; arrow in H: outer limiting membrane; stars in C,D,F: ectopic retinal cells; arrowheads in I: RGCs. OL, outer layer; IL, inner layer; ONL, outer nuclear layer; INL inner nuclear layer; RGC, retinal ganglion cells. Scale bars : A, 250 μm; B, 150 μm; E, 300 μm; C,D,F,G,H,I,J, 100 μm.
Syntaxin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/syntaxin/product/Santa Cruz Biotechnology
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Price from $9.99 to $1999.99
syntaxin - by Bioz Stars, 2024-07
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1) Product Images from "Overexpression of Pax6 results in microphthalmia, retinal dysplasia and defective retinal ganglion cell axon guidance"

Article Title: Overexpression of Pax6 results in microphthalmia, retinal dysplasia and defective retinal ganglion cell axon guidance

Journal: BMC Developmental Biology

doi: 10.1186/1471-213X-8-59

The different retinal cell types are produced normally in the PAX77 +/+ eye. Horizontal sections through wild-type and PAX77 +/+ eyes. (A) Expression of the RGC marker Brn3a at E18.5. (B) BrdU labelling (brown) of retinal progenitors in S-phase in E18.5 eyes. Sections are counterstained with cresyl violet. Expression of (C, J) Pax6 (green) and GFAP (red), (D) Islet1, (E) Chx10, (F) Syntaxin, (G) R-cadherin, (H) N-cadherin and (I) Brn3a in the retinae of wild-type and PAX77 +/+ pups at P6 (C to I) and P14 (J). (C,F) Sections are counterstained with TOPRO3. Arrowheads in C,D,F: amacrine cells; arrows in C,G: horizontal cells; arrows in E: bipolar and progenitor cells; arrowhead in G: outer plexiform layer; arrow in H: outer limiting membrane; stars in C,D,F: ectopic retinal cells; arrowheads in I: RGCs. OL, outer layer; IL, inner layer; ONL, outer nuclear layer; INL inner nuclear layer; RGC, retinal ganglion cells. Scale bars : A, 250 μm; B, 150 μm; E, 300 μm; C,D,F,G,H,I,J, 100 μm.
Figure Legend Snippet: The different retinal cell types are produced normally in the PAX77 +/+ eye. Horizontal sections through wild-type and PAX77 +/+ eyes. (A) Expression of the RGC marker Brn3a at E18.5. (B) BrdU labelling (brown) of retinal progenitors in S-phase in E18.5 eyes. Sections are counterstained with cresyl violet. Expression of (C, J) Pax6 (green) and GFAP (red), (D) Islet1, (E) Chx10, (F) Syntaxin, (G) R-cadherin, (H) N-cadherin and (I) Brn3a in the retinae of wild-type and PAX77 +/+ pups at P6 (C to I) and P14 (J). (C,F) Sections are counterstained with TOPRO3. Arrowheads in C,D,F: amacrine cells; arrows in C,G: horizontal cells; arrows in E: bipolar and progenitor cells; arrowhead in G: outer plexiform layer; arrow in H: outer limiting membrane; stars in C,D,F: ectopic retinal cells; arrowheads in I: RGCs. OL, outer layer; IL, inner layer; ONL, outer nuclear layer; INL inner nuclear layer; RGC, retinal ganglion cells. Scale bars : A, 250 μm; B, 150 μm; E, 300 μm; C,D,F,G,H,I,J, 100 μm.

Techniques Used: Produced, Expressing, Marker


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Santa Cruz Biotechnology syntaxin 1a hpc 1
Brains from mice fed with a standard diet were sliced following 14 days of tungstate treatment and were prepared for immunoreactivity assays. (A) c-fos antibody reveals an increase in c-fos immunoreactive neurons after tungstate treatment, (B) as does the GFAP antibody. (C) The β-tubulin antibody and (D) <t>HPC-1</t> show a decrease in immunoreactive neurons for these proteins. (E) NeuroD-1 immunoreactivity and (F) SNAP25 immunoreactivity increase after treatment. Scale bar = 50 µM. White and black bars represent untreated and treated animals, respectively. ** p <0.01; *** p <0.001. Data are the mean±SD.
Syntaxin 1a Hpc 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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syntaxin 1a hpc 1 - by Bioz Stars, 2024-07
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1) Product Images from "Anti-Obesity Sodium Tungstate Treatment Triggers Axonal and Glial Plasticity in Hypothalamic Feeding Centers"

Article Title: Anti-Obesity Sodium Tungstate Treatment Triggers Axonal and Glial Plasticity in Hypothalamic Feeding Centers

Journal: PLoS ONE

doi: 10.1371/journal.pone.0039087

Brains from mice fed with a standard diet were sliced following 14 days of tungstate treatment and were prepared for immunoreactivity assays. (A) c-fos antibody reveals an increase in c-fos immunoreactive neurons after tungstate treatment, (B) as does the GFAP antibody. (C) The β-tubulin antibody and (D) HPC-1 show a decrease in immunoreactive neurons for these proteins. (E) NeuroD-1 immunoreactivity and (F) SNAP25 immunoreactivity increase after treatment. Scale bar = 50 µM. White and black bars represent untreated and treated animals, respectively. ** p <0.01; *** p <0.001. Data are the mean±SD.
Figure Legend Snippet: Brains from mice fed with a standard diet were sliced following 14 days of tungstate treatment and were prepared for immunoreactivity assays. (A) c-fos antibody reveals an increase in c-fos immunoreactive neurons after tungstate treatment, (B) as does the GFAP antibody. (C) The β-tubulin antibody and (D) HPC-1 show a decrease in immunoreactive neurons for these proteins. (E) NeuroD-1 immunoreactivity and (F) SNAP25 immunoreactivity increase after treatment. Scale bar = 50 µM. White and black bars represent untreated and treated animals, respectively. ** p <0.01; *** p <0.001. Data are the mean±SD.

Techniques Used:


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Santa Cruz Biotechnology syntaxin
Syntaxin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/syntaxin/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
syntaxin - by Bioz Stars, 2024-07
93/100 stars

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Santa Cruz Biotechnology monoclonal antibody against stx 1
Monoclonal Antibody Against Stx 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Tomm20, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology stx1 syntaxin 1
Stx1 Syntaxin 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology syntaxin
Syntaxin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/syntaxin/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
syntaxin - by Bioz Stars, 2024-07
93/100 stars

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    Santa Cruz Biotechnology syntaxin 1a
    Syntaxin 1a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Primer sequences for real-time reverse transcription polymerase chain reaction.
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    The different retinal cell types are produced normally in the PAX77 +/+ eye. Horizontal sections through wild-type and PAX77 +/+ eyes. (A) Expression of the RGC marker Brn3a at E18.5. (B) BrdU labelling (brown) of retinal progenitors in S-phase in E18.5 eyes. Sections are counterstained with cresyl violet. Expression of (C, J) Pax6 (green) and GFAP (red), <t>(D)</t> <t>Islet1,</t> (E) Chx10, (F) <t>Syntaxin,</t> (G) R-cadherin, (H) N-cadherin and (I) Brn3a in the retinae of wild-type and PAX77 +/+ pups at P6 (C to I) and P14 (J). (C,F) Sections are counterstained with TOPRO3. Arrowheads in C,D,F: amacrine cells; arrows in C,G: horizontal cells; arrows in E: bipolar and progenitor cells; arrowhead in G: outer plexiform layer; arrow in H: outer limiting membrane; stars in C,D,F: ectopic retinal cells; arrowheads in I: RGCs. OL, outer layer; IL, inner layer; ONL, outer nuclear layer; INL inner nuclear layer; RGC, retinal ganglion cells. Scale bars : A, 250 μm; B, 150 μm; E, 300 μm; C,D,F,G,H,I,J, 100 μm.
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    Brains from mice fed with a standard diet were sliced following 14 days of tungstate treatment and were prepared for immunoreactivity assays. (A) c-fos antibody reveals an increase in c-fos immunoreactive neurons after tungstate treatment, (B) as does the GFAP antibody. (C) The β-tubulin antibody and (D) <t>HPC-1</t> show a decrease in immunoreactive neurons for these proteins. (E) NeuroD-1 immunoreactivity and (F) SNAP25 immunoreactivity increase after treatment. Scale bar = 50 µM. White and black bars represent untreated and treated animals, respectively. ** p <0.01; *** p <0.001. Data are the mean±SD.
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    Brains from mice fed with a standard diet were sliced following 14 days of tungstate treatment and were prepared for immunoreactivity assays. (A) c-fos antibody reveals an increase in c-fos immunoreactive neurons after tungstate treatment, (B) as does the GFAP antibody. (C) The β-tubulin antibody and (D) <t>HPC-1</t> show a decrease in immunoreactive neurons for these proteins. (E) NeuroD-1 immunoreactivity and (F) SNAP25 immunoreactivity increase after treatment. Scale bar = 50 µM. White and black bars represent untreated and treated animals, respectively. ** p <0.01; *** p <0.001. Data are the mean±SD.
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    Brains from mice fed with a standard diet were sliced following 14 days of tungstate treatment and were prepared for immunoreactivity assays. (A) c-fos antibody reveals an increase in c-fos immunoreactive neurons after tungstate treatment, (B) as does the GFAP antibody. (C) The β-tubulin antibody and (D) <t>HPC-1</t> show a decrease in immunoreactive neurons for these proteins. (E) NeuroD-1 immunoreactivity and (F) SNAP25 immunoreactivity increase after treatment. Scale bar = 50 µM. White and black bars represent untreated and treated animals, respectively. ** p <0.01; *** p <0.001. Data are the mean±SD.
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    Brains from mice fed with a standard diet were sliced following 14 days of tungstate treatment and were prepared for immunoreactivity assays. (A) c-fos antibody reveals an increase in c-fos immunoreactive neurons after tungstate treatment, (B) as does the GFAP antibody. (C) The β-tubulin antibody and (D) <t>HPC-1</t> show a decrease in immunoreactive neurons for these proteins. (E) NeuroD-1 immunoreactivity and (F) SNAP25 immunoreactivity increase after treatment. Scale bar = 50 µM. White and black bars represent untreated and treated animals, respectively. ** p <0.01; *** p <0.001. Data are the mean±SD.
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    Primer sequences for real-time reverse transcription polymerase chain reaction.

    Journal: Molecular Vision

    Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

    doi:

    Figure Lengend Snippet: Primer sequences for real-time reverse transcription polymerase chain reaction.

    Article Snippet: Twenty-four hours after seeding, cells were fixed with 4% paraformaldehyde for 30 min, treated with 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO) in 0.1% Na-Citrate (Sigma-Aldrich) for 10 min, and then blocked with 2% bovine serum albumin (Sigma-Aldrich) for 1 h. Cells were incubated with astrocyte-specific antimouse glial fibrillary acidic protein (GFAP) antibody (1:100 dilution; Abcam) and amacrine-specific antimouse syntaxin 1 antibody (1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) overnight at 4 °C.

    Techniques: Sequencing

    Primary mouse retinal ganglion cells isolated with two-step immunopanning. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

    Journal: Molecular Vision

    Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

    doi:

    Figure Lengend Snippet: Primary mouse retinal ganglion cells isolated with two-step immunopanning. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

    Article Snippet: Twenty-four hours after seeding, cells were fixed with 4% paraformaldehyde for 30 min, treated with 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO) in 0.1% Na-Citrate (Sigma-Aldrich) for 10 min, and then blocked with 2% bovine serum albumin (Sigma-Aldrich) for 1 h. Cells were incubated with astrocyte-specific antimouse glial fibrillary acidic protein (GFAP) antibody (1:100 dilution; Abcam) and amacrine-specific antimouse syntaxin 1 antibody (1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) overnight at 4 °C.

    Techniques: Isolation, Labeling, Fluorescence, Staining, Construct

    Primary mouse retinal ganglion cells isolated by direct magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

    Journal: Molecular Vision

    Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

    doi:

    Figure Lengend Snippet: Primary mouse retinal ganglion cells isolated by direct magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

    Article Snippet: Twenty-four hours after seeding, cells were fixed with 4% paraformaldehyde for 30 min, treated with 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO) in 0.1% Na-Citrate (Sigma-Aldrich) for 10 min, and then blocked with 2% bovine serum albumin (Sigma-Aldrich) for 1 h. Cells were incubated with astrocyte-specific antimouse glial fibrillary acidic protein (GFAP) antibody (1:100 dilution; Abcam) and amacrine-specific antimouse syntaxin 1 antibody (1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) overnight at 4 °C.

    Techniques: Isolation, Labeling, Fluorescence, Staining, Construct

    Primary mouse retinal ganglion cells isolated by immunopanning-magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

    Journal: Molecular Vision

    Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

    doi:

    Figure Lengend Snippet: Primary mouse retinal ganglion cells isolated by immunopanning-magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

    Article Snippet: Twenty-four hours after seeding, cells were fixed with 4% paraformaldehyde for 30 min, treated with 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO) in 0.1% Na-Citrate (Sigma-Aldrich) for 10 min, and then blocked with 2% bovine serum albumin (Sigma-Aldrich) for 1 h. Cells were incubated with astrocyte-specific antimouse glial fibrillary acidic protein (GFAP) antibody (1:100 dilution; Abcam) and amacrine-specific antimouse syntaxin 1 antibody (1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) overnight at 4 °C.

    Techniques: Isolation, Labeling, Fluorescence, Staining, Construct

    Representative western immunoblots for glial fibrillary acidic protein, syntaxin 1, and β-actin. Primary mouse retinal ganglion cells were isolated using three different systems, including two-step immunopanning, direct magnetic separation, and immunopanning-magnetic separation.

    Journal: Molecular Vision

    Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

    doi:

    Figure Lengend Snippet: Representative western immunoblots for glial fibrillary acidic protein, syntaxin 1, and β-actin. Primary mouse retinal ganglion cells were isolated using three different systems, including two-step immunopanning, direct magnetic separation, and immunopanning-magnetic separation.

    Article Snippet: Twenty-four hours after seeding, cells were fixed with 4% paraformaldehyde for 30 min, treated with 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO) in 0.1% Na-Citrate (Sigma-Aldrich) for 10 min, and then blocked with 2% bovine serum albumin (Sigma-Aldrich) for 1 h. Cells were incubated with astrocyte-specific antimouse glial fibrillary acidic protein (GFAP) antibody (1:100 dilution; Abcam) and amacrine-specific antimouse syntaxin 1 antibody (1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) overnight at 4 °C.

    Techniques: Western Blot, Isolation

    Quantitative data of real-time reverse transcription polymerase chain reaction (RT-PCR) for glial fibrillary acidic protein (GFAP) and syntaxin 1. A relative RNA ratio was calculated by dividing the value of each purification method by the value of whole retinal cell suspension. Data were expressed as the mean±SEM. (n=9 for each method). The p value for GFAP and syntaxin 1 was 0.021* and 0.022**, respectively.

    Journal: Molecular Vision

    Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

    doi:

    Figure Lengend Snippet: Quantitative data of real-time reverse transcription polymerase chain reaction (RT-PCR) for glial fibrillary acidic protein (GFAP) and syntaxin 1. A relative RNA ratio was calculated by dividing the value of each purification method by the value of whole retinal cell suspension. Data were expressed as the mean±SEM. (n=9 for each method). The p value for GFAP and syntaxin 1 was 0.021* and 0.022**, respectively.

    Article Snippet: Twenty-four hours after seeding, cells were fixed with 4% paraformaldehyde for 30 min, treated with 0.1% Triton X-100 (Sigma-Aldrich, St. Louis, MO) in 0.1% Na-Citrate (Sigma-Aldrich) for 10 min, and then blocked with 2% bovine serum albumin (Sigma-Aldrich) for 1 h. Cells were incubated with astrocyte-specific antimouse glial fibrillary acidic protein (GFAP) antibody (1:100 dilution; Abcam) and amacrine-specific antimouse syntaxin 1 antibody (1:50 dilution; Santa Cruz Biotechnology, Santa Cruz, CA) overnight at 4 °C.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Purification

    Primer sequences for real-time reverse transcription polymerase chain reaction.

    Journal: Molecular Vision

    Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

    doi:

    Figure Lengend Snippet: Primer sequences for real-time reverse transcription polymerase chain reaction.

    Article Snippet: The proteins were transferred to polyvinylidene fluoride membranes and incubated overnight with antibodies against either GFAP (Abcam), syntaxin 1 (Santa Cruz Biotechnology), or β-actin (1:1000 dilution; Santa Cruz Biotechnology).

    Techniques: Sequencing

    Primary mouse retinal ganglion cells isolated with two-step immunopanning. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

    Journal: Molecular Vision

    Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

    doi:

    Figure Lengend Snippet: Primary mouse retinal ganglion cells isolated with two-step immunopanning. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

    Article Snippet: The proteins were transferred to polyvinylidene fluoride membranes and incubated overnight with antibodies against either GFAP (Abcam), syntaxin 1 (Santa Cruz Biotechnology), or β-actin (1:1000 dilution; Santa Cruz Biotechnology).

    Techniques: Isolation, Labeling, Fluorescence, Staining, Construct

    Primary mouse retinal ganglion cells isolated by direct magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

    Journal: Molecular Vision

    Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

    doi:

    Figure Lengend Snippet: Primary mouse retinal ganglion cells isolated by direct magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

    Article Snippet: The proteins were transferred to polyvinylidene fluoride membranes and incubated overnight with antibodies against either GFAP (Abcam), syntaxin 1 (Santa Cruz Biotechnology), or β-actin (1:1000 dilution; Santa Cruz Biotechnology).

    Techniques: Isolation, Labeling, Fluorescence, Staining, Construct

    Primary mouse retinal ganglion cells isolated by immunopanning-magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

    Journal: Molecular Vision

    Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

    doi:

    Figure Lengend Snippet: Primary mouse retinal ganglion cells isolated by immunopanning-magnetic separation. Glial fibrillary acidic protein–labeled cells were detected with green fluorescence ( B ). Syntaxin 1-labeled cells were detected with red fluorescence ( C ). 4',6-diamidino-2-phenylindole nuclear staining was detected with blue fluorescence ( D ). Merge image was constructed ( A ). Scale bars: 100 μm.

    Article Snippet: The proteins were transferred to polyvinylidene fluoride membranes and incubated overnight with antibodies against either GFAP (Abcam), syntaxin 1 (Santa Cruz Biotechnology), or β-actin (1:1000 dilution; Santa Cruz Biotechnology).

    Techniques: Isolation, Labeling, Fluorescence, Staining, Construct

    Representative western immunoblots for glial fibrillary acidic protein, syntaxin 1, and β-actin. Primary mouse retinal ganglion cells were isolated using three different systems, including two-step immunopanning, direct magnetic separation, and immunopanning-magnetic separation.

    Journal: Molecular Vision

    Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

    doi:

    Figure Lengend Snippet: Representative western immunoblots for glial fibrillary acidic protein, syntaxin 1, and β-actin. Primary mouse retinal ganglion cells were isolated using three different systems, including two-step immunopanning, direct magnetic separation, and immunopanning-magnetic separation.

    Article Snippet: The proteins were transferred to polyvinylidene fluoride membranes and incubated overnight with antibodies against either GFAP (Abcam), syntaxin 1 (Santa Cruz Biotechnology), or β-actin (1:1000 dilution; Santa Cruz Biotechnology).

    Techniques: Western Blot, Isolation

    Quantitative data of real-time reverse transcription polymerase chain reaction (RT-PCR) for glial fibrillary acidic protein (GFAP) and syntaxin 1. A relative RNA ratio was calculated by dividing the value of each purification method by the value of whole retinal cell suspension. Data were expressed as the mean±SEM. (n=9 for each method). The p value for GFAP and syntaxin 1 was 0.021* and 0.022**, respectively.

    Journal: Molecular Vision

    Article Title: Isolation of primary mouse retinal ganglion cells using immunopanning-magnetic separation

    doi:

    Figure Lengend Snippet: Quantitative data of real-time reverse transcription polymerase chain reaction (RT-PCR) for glial fibrillary acidic protein (GFAP) and syntaxin 1. A relative RNA ratio was calculated by dividing the value of each purification method by the value of whole retinal cell suspension. Data were expressed as the mean±SEM. (n=9 for each method). The p value for GFAP and syntaxin 1 was 0.021* and 0.022**, respectively.

    Article Snippet: The proteins were transferred to polyvinylidene fluoride membranes and incubated overnight with antibodies against either GFAP (Abcam), syntaxin 1 (Santa Cruz Biotechnology), or β-actin (1:1000 dilution; Santa Cruz Biotechnology).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Purification

    The different retinal cell types are produced normally in the PAX77 +/+ eye. Horizontal sections through wild-type and PAX77 +/+ eyes. (A) Expression of the RGC marker Brn3a at E18.5. (B) BrdU labelling (brown) of retinal progenitors in S-phase in E18.5 eyes. Sections are counterstained with cresyl violet. Expression of (C, J) Pax6 (green) and GFAP (red), (D) Islet1, (E) Chx10, (F) Syntaxin, (G) R-cadherin, (H) N-cadherin and (I) Brn3a in the retinae of wild-type and PAX77 +/+ pups at P6 (C to I) and P14 (J). (C,F) Sections are counterstained with TOPRO3. Arrowheads in C,D,F: amacrine cells; arrows in C,G: horizontal cells; arrows in E: bipolar and progenitor cells; arrowhead in G: outer plexiform layer; arrow in H: outer limiting membrane; stars in C,D,F: ectopic retinal cells; arrowheads in I: RGCs. OL, outer layer; IL, inner layer; ONL, outer nuclear layer; INL inner nuclear layer; RGC, retinal ganglion cells. Scale bars : A, 250 μm; B, 150 μm; E, 300 μm; C,D,F,G,H,I,J, 100 μm.

    Journal: BMC Developmental Biology

    Article Title: Overexpression of Pax6 results in microphthalmia, retinal dysplasia and defective retinal ganglion cell axon guidance

    doi: 10.1186/1471-213X-8-59

    Figure Lengend Snippet: The different retinal cell types are produced normally in the PAX77 +/+ eye. Horizontal sections through wild-type and PAX77 +/+ eyes. (A) Expression of the RGC marker Brn3a at E18.5. (B) BrdU labelling (brown) of retinal progenitors in S-phase in E18.5 eyes. Sections are counterstained with cresyl violet. Expression of (C, J) Pax6 (green) and GFAP (red), (D) Islet1, (E) Chx10, (F) Syntaxin, (G) R-cadherin, (H) N-cadherin and (I) Brn3a in the retinae of wild-type and PAX77 +/+ pups at P6 (C to I) and P14 (J). (C,F) Sections are counterstained with TOPRO3. Arrowheads in C,D,F: amacrine cells; arrows in C,G: horizontal cells; arrows in E: bipolar and progenitor cells; arrowhead in G: outer plexiform layer; arrow in H: outer limiting membrane; stars in C,D,F: ectopic retinal cells; arrowheads in I: RGCs. OL, outer layer; IL, inner layer; ONL, outer nuclear layer; INL inner nuclear layer; RGC, retinal ganglion cells. Scale bars : A, 250 μm; B, 150 μm; E, 300 μm; C,D,F,G,H,I,J, 100 μm.

    Article Snippet: Primary antibodies were: Pax6 (1:400, DSHB), GFAP (1:50, Dako), Brn3a (1:300, Chemicon; MAB1585), Islet1 (1:50, DSHB), Ncad (1:500, BD), BrdU (1:200, Becton Dickinson), Syntaxin (1:100, Santa-Cruz sc-12736), Chx10 (1:1000, a gift from C. Cepko), Pax2 (1:200, Cambridge Bioscience), Rcad (1:100, a gift from M. Takeichi), Neurofilament (1:100, BIOMOL, USA; NA1297), L1 (1:50, Chemicon; MAB5272).

    Techniques: Produced, Expressing, Marker

    Brains from mice fed with a standard diet were sliced following 14 days of tungstate treatment and were prepared for immunoreactivity assays. (A) c-fos antibody reveals an increase in c-fos immunoreactive neurons after tungstate treatment, (B) as does the GFAP antibody. (C) The β-tubulin antibody and (D) HPC-1 show a decrease in immunoreactive neurons for these proteins. (E) NeuroD-1 immunoreactivity and (F) SNAP25 immunoreactivity increase after treatment. Scale bar = 50 µM. White and black bars represent untreated and treated animals, respectively. ** p <0.01; *** p <0.001. Data are the mean±SD.

    Journal: PLoS ONE

    Article Title: Anti-Obesity Sodium Tungstate Treatment Triggers Axonal and Glial Plasticity in Hypothalamic Feeding Centers

    doi: 10.1371/journal.pone.0039087

    Figure Lengend Snippet: Brains from mice fed with a standard diet were sliced following 14 days of tungstate treatment and were prepared for immunoreactivity assays. (A) c-fos antibody reveals an increase in c-fos immunoreactive neurons after tungstate treatment, (B) as does the GFAP antibody. (C) The β-tubulin antibody and (D) HPC-1 show a decrease in immunoreactive neurons for these proteins. (E) NeuroD-1 immunoreactivity and (F) SNAP25 immunoreactivity increase after treatment. Scale bar = 50 µM. White and black bars represent untreated and treated animals, respectively. ** p <0.01; *** p <0.001. Data are the mean±SD.

    Article Snippet: The sections were incubated overnight with primary antibodies: c-fos, syntaxin 1A (HPC-1) 1∶100 (Santa Cruz Biotechnology, Santa Cruz, CA, USA); β-tubulin 1∶200, synaptosomal-associated protein 25 (SNAP-25) 1∶100, collapsing response mediator protein 2 (CRMP2) 1∶50 (Abcam, Cambridge, United Kingdom); glial fibrillary acidic protein (GFAP) 1∶100 (Sigma-Aldrich); and neurogenic differentiation factor 1 (NeuroD1) 1∶200 (Chemicon, Temecula, CA, USA).

    Techniques: