5637 human bladder epithelial cells  (ATCC)


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    ATCC 5637 human bladder epithelial cells
    5637 Human Bladder Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5637 human bladder epithelial cells/product/ATCC
    Average 97 stars, based on 1 article reviews
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    5637 human bladder epithelial cells - by Bioz Stars, 2023-01
    97/100 stars

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    5637 human bladder epithelial cells  (ATCC)


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    ATCC 5637 human bladder epithelial cells
    5637 Human Bladder Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5637 human bladder epithelial cells/product/ATCC
    Average 97 stars, based on 1 article reviews
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    5637 human bladder epithelial cells - by Bioz Stars, 2023-01
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    human bladder epithelial cell line 5637  (ATCC)


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    ATCC human bladder epithelial cell line 5637
    Human Bladder Epithelial Cell Line 5637, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human bladder epithelial cell line 5637/product/ATCC
    Average 97 stars, based on 1 article reviews
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    human bladder epithelial cell line 5637 - by Bioz Stars, 2023-01
    97/100 stars

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    human bladder cancer cell lines 5637  (ATCC)


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    ATCC human bladder cancer cell lines 5637
    Human Bladder Cancer Cell Lines 5637, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human bladder cancer cell lines 5637/product/ATCC
    Average 97 stars, based on 1 article reviews
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    human bladder cancer cell lines 5637 - by Bioz Stars, 2023-01
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    human 5637  (ATCC)


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    ATCC human 5637
    DHA exposure significantly decreased human bladder cancer cell viability in dose- and time-dependent manner. Human 5637, UMUC3 and T24 bladder cancer cells as well as human SV-HUC-1 uroepithelial cells cells were seeded in 96-well plate for 24 hours, subsequently, cells were treated with 50 to 400 μM of DHA or DMSO. Cells were then collected at designated time points for CCK-8 analysis. Values represented the means ± SEM for three to four independent experiments. Statistical comparisons were made between the DHA-treated groups versus DMSO-treated groups. NS, not significant; **, P < 0.01 ***, P < 0.001; ****, P < 0.0001. (A-D) DHA exposure significantly decreased SV-HUC-1, 5637, UMUC3 and T24 cell viability in a dose-dependent manner, as determined by CCK-8 assay. (E) DHA exposure significantly decreased T24 cell viability in a time-dependent manner, as determined by CCK-8 assay.
    Human 5637, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 1 article reviews
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    human 5637 - by Bioz Stars, 2023-01
    97/100 stars

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    1) Product Images from "Dihydroartemisinin suppresses bladder cancer cell invasion and migration by regulating KDM3A and p21"

    Article Title: Dihydroartemisinin suppresses bladder cancer cell invasion and migration by regulating KDM3A and p21

    Journal: Journal of Cancer

    doi: 10.7150/jca.36174

    DHA exposure significantly decreased human bladder cancer cell viability in dose- and time-dependent manner. Human 5637, UMUC3 and T24 bladder cancer cells as well as human SV-HUC-1 uroepithelial cells cells were seeded in 96-well plate for 24 hours, subsequently, cells were treated with 50 to 400 μM of DHA or DMSO. Cells were then collected at designated time points for CCK-8 analysis. Values represented the means ± SEM for three to four independent experiments. Statistical comparisons were made between the DHA-treated groups versus DMSO-treated groups. NS, not significant; **, P < 0.01 ***, P < 0.001; ****, P < 0.0001. (A-D) DHA exposure significantly decreased SV-HUC-1, 5637, UMUC3 and T24 cell viability in a dose-dependent manner, as determined by CCK-8 assay. (E) DHA exposure significantly decreased T24 cell viability in a time-dependent manner, as determined by CCK-8 assay.
    Figure Legend Snippet: DHA exposure significantly decreased human bladder cancer cell viability in dose- and time-dependent manner. Human 5637, UMUC3 and T24 bladder cancer cells as well as human SV-HUC-1 uroepithelial cells cells were seeded in 96-well plate for 24 hours, subsequently, cells were treated with 50 to 400 μM of DHA or DMSO. Cells were then collected at designated time points for CCK-8 analysis. Values represented the means ± SEM for three to four independent experiments. Statistical comparisons were made between the DHA-treated groups versus DMSO-treated groups. NS, not significant; **, P < 0.01 ***, P < 0.001; ****, P < 0.0001. (A-D) DHA exposure significantly decreased SV-HUC-1, 5637, UMUC3 and T24 cell viability in a dose-dependent manner, as determined by CCK-8 assay. (E) DHA exposure significantly decreased T24 cell viability in a time-dependent manner, as determined by CCK-8 assay.

    Techniques Used: CCK-8 Assay

    lines 5637 bladder carcinoma  (ATCC)


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    ATCC lines 5637 bladder carcinoma
    Lines 5637 Bladder Carcinoma, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    5637 human bladder cells  (ATCC)


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    ATCC 5637 human bladder cells
    5637 Human Bladder Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5637 human bladder cells/product/ATCC
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    mccoy s 5a medium  (ATCC)


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    ATCC mccoy s 5a medium
    Mccoy S 5a Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bca cell lines 5637  (ATCC)


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    ATCC bca cell lines 5637
    Bca Cell Lines 5637, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human bca cell lines  (ATCC)


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    ATCC human bca cell lines
    EDU assay was performed to investigate cell growth. a, d, g Estrogen promoted the multiplication <t>of</t> <t>5637</t> and <t>T24</t> cells. b, e, h Downregulation of P2RY2e by Cas13a-P2RY2e inhibit cell proliferation of 5637 and T24 cells. c, f, i Downregulation of P2RY2e by Cas13a-P2RY2e decreased cell proliferation induced by estrogen. E2 represented estrogen. Data are shown as mean ± SD. (*p<0.05, **p< 0.01)
    Human Bca Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 97 stars, based on 1 article reviews
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    human bca cell lines - by Bioz Stars, 2023-01
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    1) Product Images from "Enhancer RNA - P2RY2e induced by estrogen promotes malignant behaviors of bladder cancer"

    Article Title: Enhancer RNA - P2RY2e induced by estrogen promotes malignant behaviors of bladder cancer

    Journal: International Journal of Biological Sciences

    doi: 10.7150/ijbs.27151

    EDU assay was performed to investigate cell growth. a, d, g Estrogen promoted the multiplication of 5637 and T24 cells. b, e, h Downregulation of P2RY2e by Cas13a-P2RY2e inhibit cell proliferation of 5637 and T24 cells. c, f, i Downregulation of P2RY2e by Cas13a-P2RY2e decreased cell proliferation induced by estrogen. E2 represented estrogen. Data are shown as mean ± SD. (*p<0.05, **p< 0.01)
    Figure Legend Snippet: EDU assay was performed to investigate cell growth. a, d, g Estrogen promoted the multiplication of 5637 and T24 cells. b, e, h Downregulation of P2RY2e by Cas13a-P2RY2e inhibit cell proliferation of 5637 and T24 cells. c, f, i Downregulation of P2RY2e by Cas13a-P2RY2e decreased cell proliferation induced by estrogen. E2 represented estrogen. Data are shown as mean ± SD. (*p<0.05, **p< 0.01)

    Techniques Used: EdU Assay

    CCK-8 assay was used to detect cell proliferation as well. a Increased cell growth was observed in 5637 and T24 cells with stimulation of estrogen (10mM). b Cell proliferation suppression was observed in 5637 and T24 cells transfected with Cas13a-P2RY2e. c Estrogen-induced proliferation was attenuated after transfection with Cas13a-P2RY2e in 5637 and T24. Transwell assay was used to detect cell invasion. d, g Estrogen promoted the cell invasion of 5637 and T24 cells. e, h Cell invasion was inhibit in 5637 and T24 after transfection of Cas13a-P2RY2e. f, i Compared with the negative control, cell invasion was inhibit with estrogen treatment and Cas13a-P2RY2e transfection. E2 represented estrogen. Data are shown as mean ± SD. (*p<0.05, **p< 0.01, ***p< 0.001)
    Figure Legend Snippet: CCK-8 assay was used to detect cell proliferation as well. a Increased cell growth was observed in 5637 and T24 cells with stimulation of estrogen (10mM). b Cell proliferation suppression was observed in 5637 and T24 cells transfected with Cas13a-P2RY2e. c Estrogen-induced proliferation was attenuated after transfection with Cas13a-P2RY2e in 5637 and T24. Transwell assay was used to detect cell invasion. d, g Estrogen promoted the cell invasion of 5637 and T24 cells. e, h Cell invasion was inhibit in 5637 and T24 after transfection of Cas13a-P2RY2e. f, i Compared with the negative control, cell invasion was inhibit with estrogen treatment and Cas13a-P2RY2e transfection. E2 represented estrogen. Data are shown as mean ± SD. (*p<0.05, **p< 0.01, ***p< 0.001)

    Techniques Used: CCK-8 Assay, Transfection, Transwell Assay, Negative Control

    Cell migration was analyzed by wound-healing assay. a, d, g Estrogen promoted the cell migration of 5637 and T24 cells. b, e, h After transfection of Cas13a-P2RY2e, cell migration was inhibit in 5637 and T24. c, f , i Cell migration was inhibit with estrogen treatment and Cas13a-P2RY2e transfection compared to the negative control. E2 represented estrogen. Data are shown as mean ± SD. (*p<0.05, **p< 0.01)
    Figure Legend Snippet: Cell migration was analyzed by wound-healing assay. a, d, g Estrogen promoted the cell migration of 5637 and T24 cells. b, e, h After transfection of Cas13a-P2RY2e, cell migration was inhibit in 5637 and T24. c, f , i Cell migration was inhibit with estrogen treatment and Cas13a-P2RY2e transfection compared to the negative control. E2 represented estrogen. Data are shown as mean ± SD. (*p<0.05, **p< 0.01)

    Techniques Used: Migration, Wound Healing Assay, Transfection, Negative Control

    Cell apoptosis was measured by caspase-3 ELISA assay and flow cytometry assay. a, d . g Estrogen decreased the cell apoptosis of 5637 and T24 cells. b, e, h P2RY2e downregulation increased cell apoptosis of 5637 and T24 cells. c, f, i Cell apoptosis was facilitated with estrogen treatment and Cas13a-P2RY2e transfection compared to the negative control. E2 represented estrogen. Data are shown as mean ± SD. (*p<0.05, **p< 0.01, ***p< 0.001)
    Figure Legend Snippet: Cell apoptosis was measured by caspase-3 ELISA assay and flow cytometry assay. a, d . g Estrogen decreased the cell apoptosis of 5637 and T24 cells. b, e, h P2RY2e downregulation increased cell apoptosis of 5637 and T24 cells. c, f, i Cell apoptosis was facilitated with estrogen treatment and Cas13a-P2RY2e transfection compared to the negative control. E2 represented estrogen. Data are shown as mean ± SD. (*p<0.05, **p< 0.01, ***p< 0.001)

    Techniques Used: Enzyme-linked Immunosorbent Assay, Flow Cytometry, Transfection, Negative Control

    bladder cancer cell lines 5637  (ATCC)


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    ATCC bladder cancer cell lines 5637
    (A) RT-PCR was performed using sense and antisense primers for S100A2 for 10 head/neck cancer cell lines (011, 012, 013, 019, 022, 028, Fadu, KYSE30, KYSE410, and KYSE520) and 4 bladder cancer cell lines (5637, HT1763, J82, and SCaBER). The name of each cell line is marked on the top of each lane, and the size of PCR product is marked with an arrow. PCR mixtures without templates were used as negative controls (H 0). GAPDH was the loading control. Each PCR product was cloned and its sequence confirmed. Eight of the 14 cell lines did not demonstrate expression of S100A2 . (B) Western blot analysis was performed using antibodies for S100A2 for the same 14 cancer cell lines. The name of each cell line is marked on the top of each lane, and the size of the protein is marked with an arrow. MDBK cells were used as positive control for Western blots (P). β-actin was the loading control.
    Bladder Cancer Cell Lines 5637, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bladder cancer cell lines 5637 - by Bioz Stars, 2023-01
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    1) Product Images from "Epigenetic silencing of S100A2 in bladder and head and neck cancers"

    Article Title: Epigenetic silencing of S100A2 in bladder and head and neck cancers

    Journal: Oncoscience

    doi:

    (A) RT-PCR was performed using sense and antisense primers for S100A2 for 10 head/neck cancer cell lines (011, 012, 013, 019, 022, 028, Fadu, KYSE30, KYSE410, and KYSE520) and 4 bladder cancer cell lines (5637, HT1763, J82, and SCaBER). The name of each cell line is marked on the top of each lane, and the size of PCR product is marked with an arrow. PCR mixtures without templates were used as negative controls (H 0). GAPDH was the loading control. Each PCR product was cloned and its sequence confirmed. Eight of the 14 cell lines did not demonstrate expression of S100A2 . (B) Western blot analysis was performed using antibodies for S100A2 for the same 14 cancer cell lines. The name of each cell line is marked on the top of each lane, and the size of the protein is marked with an arrow. MDBK cells were used as positive control for Western blots (P). β-actin was the loading control.
    Figure Legend Snippet: (A) RT-PCR was performed using sense and antisense primers for S100A2 for 10 head/neck cancer cell lines (011, 012, 013, 019, 022, 028, Fadu, KYSE30, KYSE410, and KYSE520) and 4 bladder cancer cell lines (5637, HT1763, J82, and SCaBER). The name of each cell line is marked on the top of each lane, and the size of PCR product is marked with an arrow. PCR mixtures without templates were used as negative controls (H 0). GAPDH was the loading control. Each PCR product was cloned and its sequence confirmed. Eight of the 14 cell lines did not demonstrate expression of S100A2 . (B) Western blot analysis was performed using antibodies for S100A2 for the same 14 cancer cell lines. The name of each cell line is marked on the top of each lane, and the size of the protein is marked with an arrow. MDBK cells were used as positive control for Western blots (P). β-actin was the loading control.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Clone Assay, Sequencing, Expressing, Western Blot, Positive Control

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