nucleic acid kit 55114 (Qiagen)

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QIAamp Circulating Nucleic Acid Kit

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For isolation of free circulating DNA and RNA from human plasma or serum Kit contents Qiagen QIAamp Circulating Nucleic Acid Kit 50 preps 1 to 5mL Sample 20 to 150L Elution Volume Serum Plasma Sample Free circulating DNA RNA and miRNA Viral DNA Viral RNA Purification QIAamp Mini Column Format Silica Technology For Isolation of Free circulating DNA and RNA from Human Plasma or Serum Includes QIAamp Mini Columns 20mL Tube Extenders Qiagen Proteinase K Carrier RNA Buffers VacConnectors and 1 5mL and 2mL Collection Tubes Benefits Concentration of nucleic acids with high input and low elution volumes Efficient recovery of fragmented DNA and RNA No organic extraction or ethanol precipitation Removal of contaminants and inhibitors

Catalog Number:

55114

Price:

1091

Category:

QIAamp Circulating Nucleic Acid Kit

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Structured Review

Qiagen nucleic acid kit 55114

nucleic acid kit 55114 - by Bioz Stars, 2020-01

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Centrifugation:

Article Title: Circulating tumour DNA reflects treatment response and clonal evolution in chronic lymphocytic leukaemia
Article Snippet: Whole blood was first centrifuged at 1,600 g for 10 min (brake off) to separate the plasma from the PB cells, followed by a further centrifugation step at 20,000 g for 10 min to pellet any remaining cells and/or debris. .. DNA was extracted from 2 ml aliquots of plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen, 55114) according to the manufacturer's instructions.

Amplification:

Article Title: Circulating tumour DNA reflects treatment response and clonal evolution in chronic lymphocytic leukaemia
Article Snippet: DNA was extracted from 2 ml aliquots of plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen, 55114) according to the manufacturer's instructions. .. To estimate the efficiency of DNA extraction, equal amounts of a diluted DNA amplicon (PCR amplified from Drosophila melanogaster genomic DNA, ) was spiked into each plasma sample before extraction.

Article Title: Simple and Low‐Cost Sampling of Cell‐Free Nucleic Acids from Blood Plasma for Rapid and Sensitive Detection of Circulating Tumor DNA
Article Snippet: The DTBP platform was compared with the column‐based cfDNA isolation method (QIAamp Circulating Nucleic Acid Kit, Qiagen, Germany), which was used according to the manufacturer's protocol. .. The 18S rRNA and GAPDH genes were amplified with the isolated cfRNA and used to check the quality and quantity of cfRNA.

Quantitative RT-PCR:

Article Title: Novel blood-based microRNA biomarker panel for early diagnosis of pancreatic cancer
Article Snippet: Paragraph title: Independent validation RT-qPCR (performed at the Valley Hospital, NJ) ... MiRNA was extracted from 2.0 to 4.0 mL of plasma using QIAamp circulating Nucleic Acid Kit (Qiagen, Germantown, MD) with QIAvac Connecting System (Qiagen), following manufacturer instructions.

Real-time Polymerase Chain Reaction:

Article Title: Analysis of solid tumor mutation profiles in liquid biopsy, et al. Analysis of solid tumor mutation profiles in liquid biopsy
Article Snippet: Total cfDNA was extracted from plasma using QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat. No: 55114), as per standard protocol. .. The genomic DNA contamination in the cfDNA was established using an ALU‐based qPCR assay.

Article Title: The molecular evolution of acquired resistance to targeted EGFR blockade in colorectal cancers
Article Snippet: DNA purification DNA was purified from of 0.2–1mL of banked serum using the QIAamp circulating nucleic acid kit (Qiagen, cat. # 55114) following the manufacturer’s recommendations. .. Amplifiable DNA was quantified with qPCR, employing primers and conditions as previously described .

Incubation:

Article Title: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids
Article Snippet: Cell-free DNA isolation from urine Urinary cfDNA was isolated using QIAamp Circulating Nucleic Acid Kit (QIAGEN, 55114) following the manufacturer’s instructions. .. Briefly, urine samples were mixed with proteinase K, buffer ACL and buffer ATL, and incubated in a 60 °C water bath for 30 min. ACB binding buffer was added to the mixture and the preparation was then loaded into the mini-column.

Formalin-fixed Paraffin-Embedded:

Article Title: Evaluation of pre-analytical conditions and comparison of the performance of several digital PCR assays for the detection of major EGFR mutations in circulating DNA from non-small cell lung cancers: the CIRCAN_0 study
Article Snippet: Tumor DNA was then extracted from microdissected tissues using the QIAamp DNA FFPE Tissue Kit (Qiagen, Valencia, CA, USA), according to the manufacturer's instructions. .. CfDNA was extracted from 1 mL, 3 mL or 5 mL of plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat No 55114, Valencia, CA, USA), with a Qiagen vacuum manifold following the manufacturers' instructions.

Article Title: Clinical importance of long non-coding RNA LINC00460 expression in EGFR-mutant lung adenocarcinoma
Article Snippet: RNA was extracted from the FFPE slides using an RNeasy FFPE kit (cat. no. 73504, Qiagen) and separated serum was stored at −80°C until use. .. Cell-free RNA in peripheral blood was extracted and purified using a QIAamp Circulating Nucleic Acid kit (cat. no. 55114, Qiagen) in accordance with the manufacturer's protocol and stored at −20°C until use.

Article Title: Detection of somatic mutations and HPV in the saliva and plasma of patients with head and neck squamous cell carcinomas
Article Snippet: In either case (fresh-frozen or FFPE), tumors were macrodissected to ensure neoplastic cellularity exceeding 30%. .. DNA was purified from the white blood cell pellet (normal DNA), saliva, and tumor using an AllPrep Kit (Qiagen, catalog #80204), and from plasma using an QIAamp Circulating Nucleic Acid Kit (Qiagen, catalog #55114).

Expressing:

Article Title: Clinical importance of long non-coding RNA LINC00460 expression in EGFR-mutant lung adenocarcinoma
Article Snippet: LINC00460 expression was analyzed in 62 samples (FFPE slides of surgical specimens in 23 cases and cell-free RNA in 39 cases). .. Cell-free RNA in peripheral blood was extracted and purified using a QIAamp Circulating Nucleic Acid kit (cat. no. 55114, Qiagen) in accordance with the manufacturer's protocol and stored at −20°C until use.

Flow Cytometry:

Article Title: Simple and Low‐Cost Sampling of Cell‐Free Nucleic Acids from Blood Plasma for Rapid and Sensitive Detection of Circulating Tumor DNA
Article Snippet: After washing with phosphate‐buffered saline to remove debris from the samples using a syringe pump (100 µL min−1 ), an elution buffer (10 × 10−3 m sodium bicarbonate, pH < 10.6, flow rate 50 µL min−1 ) was used to collect the cfNAs that were isolated within a few minutes by breaking the crosslinking with DTBP and amine group of APDMS. .. The DTBP platform was compared with the column‐based cfDNA isolation method (QIAamp Circulating Nucleic Acid Kit, Qiagen, Germany), which was used according to the manufacturer's protocol.

Infection:

Article Title: Reduction of MicroRNA 122 Expression in IFNL3 CT/TT Carriers and during Progression of Fibrosis in Patients with Chronic Hepatitis C
Article Snippet: In our series, the patients were mainly of Caucasian ancestry and infected with HCV genotype 1. .. Free, circulating DNA was extracted from 500-μl serum samples (QIAamp circulating nucleic acid kit; Qiagen) as described previously ( ).

Digital PCR:

Article Title: Circulating tumour DNA reflects treatment response and clonal evolution in chronic lymphocytic leukaemia
Article Snippet: DNA was extracted from 2 ml aliquots of plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen, 55114) according to the manufacturer's instructions. .. The amount of spike-in Drosophila DNA was then quantified in each eluted plasma DNA sample by dPCR and the efficiency of extraction was determined by comparing this quantity with a no-extraction control that was prepared by re-suspension of the same amount of Drosophila DNA in the same elution medium.

Article Title: Early Change in FDG-PET Signal and Plasma Cell-Free DNA Level Predicts Erlotinib Response in EGFR Wild-Type NSCLC Patients
Article Snippet: Total cfDNA was purified from 2 ml of plasma by use of the QIAamp circulating Nucleic Acid kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol and eluted in a volume of 100 μl of TE buffer. .. The two genes were quantified in duplicates by droplet digital polymerase chain reaction (ddPCR, Bio-Rad QX200) in a multiplex reaction using 5 μl of sample.

Polymerase Chain Reaction:

Article Title: Reduction of MicroRNA 122 Expression in IFNL3 CT/TT Carriers and during Progression of Fibrosis in Patients with Chronic Hepatitis C
Article Snippet: Free, circulating DNA was extracted from 500-μl serum samples (QIAamp circulating nucleic acid kit; Qiagen) as described previously ( ). .. The PCR products were separated on an ABI 3130 sequencer and analyzed with SeqScape 2.6 (Life Technologies).

Injection:

Article Title: Simple and Low‐Cost Sampling of Cell‐Free Nucleic Acids from Blood Plasma for Rapid and Sensitive Detection of Circulating Tumor DNA
Article Snippet: The DTBP platform was compared with the column‐based cfDNA isolation method (QIAamp Circulating Nucleic Acid Kit, Qiagen, Germany), which was used according to the manufacturer's protocol. .. The samples were injected into the platform, the debris was then washed away, and cfRNA was isolated.

Binding Assay:

Article Title: Simple and Low‐Cost Sampling of Cell‐Free Nucleic Acids from Blood Plasma for Rapid and Sensitive Detection of Circulating Tumor DNA
Article Snippet: To compare the direct binding capability of cfNA, other HI groups were tested (100 mg mL−1 ), such as dimethyl adipimidate, dimethyl suberimidate, and dimethyl pimelimidate. .. The DTBP platform was compared with the column‐based cfDNA isolation method (QIAamp Circulating Nucleic Acid Kit, Qiagen, Germany), which was used according to the manufacturer's protocol.

DNA Extraction:

Article Title: Circulating tumour DNA reflects treatment response and clonal evolution in chronic lymphocytic leukaemia
Article Snippet: Paragraph title: Sample processing and DNA extraction ... DNA was extracted from 2 ml aliquots of plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen, 55114) according to the manufacturer's instructions.

Article Title: Analysis of solid tumor mutation profiles in liquid biopsy, et al. Analysis of solid tumor mutation profiles in liquid biopsy
Article Snippet: Paragraph title: Cell‐free DNA extraction from blood ... Total cfDNA was extracted from plasma using QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat. No: 55114), as per standard protocol.

Article Title: Evaluation of pre-analytical conditions and comparison of the performance of several digital PCR assays for the detection of major EGFR mutations in circulating DNA from non-small cell lung cancers: the CIRCAN_0 study
Article Snippet: Paragraph title: DNA extraction ... CfDNA was extracted from 1 mL, 3 mL or 5 mL of plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat No 55114, Valencia, CA, USA), with a Qiagen vacuum manifold following the manufacturers' instructions.

Article Title: T Oligo-Primed Polymerase Chain Reaction (TOP-PCR): A Robust Method for the Amplification of Minute DNA Fragments in Body Fluids
Article Snippet: .. Cell-free DNA isolation from urine Urinary cfDNA was isolated using QIAamp Circulating Nucleic Acid Kit (QIAGEN, 55114) following the manufacturer’s instructions. .. Briefly, urine samples were mixed with proteinase K, buffer ACL and buffer ATL, and incubated in a 60 °C water bath for 30 min. ACB binding buffer was added to the mixture and the preparation was then loaded into the mini-column.

Mutagenesis:

Article Title: Detection of somatic mutations and HPV in the saliva and plasma of patients with head and neck squamous cell carcinomas
Article Snippet: There was no significant difference in the amounts of DNA purified, the fraction of mutant DNA, or the amount of HPV sequences found with the two protocols. .. DNA was purified from the white blood cell pellet (normal DNA), saliva, and tumor using an AllPrep Kit (Qiagen, catalog #80204), and from plasma using an QIAamp Circulating Nucleic Acid Kit (Qiagen, catalog #55114).

Isolation:

Article Title: Analysis of solid tumor mutation profiles in liquid biopsy, et al. Analysis of solid tumor mutation profiles in liquid biopsy
Article Snippet: 2.3 Cell‐free DNA extraction from blood Following collection of blood in Streck tubes, plasma was isolated within 48‐72 hours of collection. .. Total cfDNA was extracted from plasma using QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat. No: 55114), as per standard protocol.

Article Title: Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pressure
Article Snippet: To extract cfDNA from a plasma sample, reagents in the QIAamp circulating nucleic acid kit (55114; QIAGEN, Germany) were utilized. .. Plasma was isolated at 1,900 g for 10 min in a centrifuge (1248; LABOGEN, Denmark) and was re-centrifuged at 12,000 g for 15 min; 500 μL of proteinase K was added to 5 mL of plasma in a 50-mL conical tube; 4 mL of lysate-buffer (ACL) containing 1.0 μg of carrier RNA was added.

Article Title: Simple and Low‐Cost Sampling of Cell‐Free Nucleic Acids from Blood Plasma for Rapid and Sensitive Detection of Circulating Tumor DNA
Article Snippet: .. The DTBP platform was compared with the column‐based cfDNA isolation method (QIAamp Circulating Nucleic Acid Kit, Qiagen, Germany), which was used according to the manufacturer's protocol. .. To identify whether cfRNA can be isolated using the DTBP platform, two blood plasma samples were mixed with DTBP and DNase (Qiagen).

Article Title: Circulating tumour DNA profiling reveals heterogeneity of EGFR inhibitor resistance mechanisms in lung cancer patients
Article Snippet: Up to 24 ml of blood was collected in K2 EDTA tubes, processed into plasma within 15±5 min (1,800g for 10 min at 18–23 °C), pooled and stored at −80 °C in 2.2 ml aliquots until cfDNA isolation. .. Briefly, cell-free DNA (cfDNA) was extracted from 2 to 4 ml (average of 3.44 ml) of plasma using the QiaAmp Circulating Nucleic Acid Kit (Qiagen, cat. no. 55114) according to manufacturer's instructions.

Purification:

Article Title: Early Change in FDG-PET Signal and Plasma Cell-Free DNA Level Predicts Erlotinib Response in EGFR Wild-Type NSCLC Patients
Article Snippet: .. Total cfDNA was purified from 2 ml of plasma by use of the QIAamp circulating Nucleic Acid kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol and eluted in a volume of 100 μl of TE buffer. ..

Article Title: The molecular evolution of acquired resistance to targeted EGFR blockade in colorectal cancers
Article Snippet: .. DNA purification DNA was purified from of 0.2–1mL of banked serum using the QIAamp circulating nucleic acid kit (Qiagen, cat. # 55114) following the manufacturer’s recommendations. .. Amplifiable DNA was quantified with qPCR, employing primers and conditions as previously described .

Article Title: Clinical importance of long non-coding RNA LINC00460 expression in EGFR-mutant lung adenocarcinoma
Article Snippet: .. Cell-free RNA in peripheral blood was extracted and purified using a QIAamp Circulating Nucleic Acid kit (cat. no. 55114, Qiagen) in accordance with the manufacturer's protocol and stored at −20°C until use. ..

Article Title: Radiotherapy increases plasma levels of tumoral cell-free DNA in non-small cell lung cancer patients
Article Snippet: .. DNA was purified using the QIAamp Circulating Nucleic Acid Kit (55114; Qiagen, Inc., Valencia, CA, USA) according to the manufacturer’s instructions. .. We eluted the DNA in 50 μL double distilled water and stored at –20° C. The DNA concentration was quantified with a Quantus fluorometer (Promega, Madison, WI, USA) following the manufacturer’s instructions.

Article Title: Detection of somatic mutations and HPV in the saliva and plasma of patients with head and neck squamous cell carcinomas
Article Snippet: .. DNA was purified from the white blood cell pellet (normal DNA), saliva, and tumor using an AllPrep Kit (Qiagen, catalog #80204), and from plasma using an QIAamp Circulating Nucleic Acid Kit (Qiagen, catalog #55114). .. A tiered approach was used to identify a somatic mutation within each tumor.

Sequencing:

Article Title: Reduction of MicroRNA 122 Expression in IFNL3 CT/TT Carriers and during Progression of Fibrosis in Patients with Chronic Hepatitis C
Article Snippet: The 133 patients included in the cohort were genotyped for rs12979860 using direct sequencing (AmpliTaq gold and BigDye Terminator; Life Technologies). .. Free, circulating DNA was extracted from 500-μl serum samples (QIAamp circulating nucleic acid kit; Qiagen) as described previously ( ).

Article Title: Circulating tumour DNA profiling reveals heterogeneity of EGFR inhibitor resistance mechanisms in lung cancer patients
Article Snippet: Circulating tumour DNA analysis was performed using Cancer Personalized Profiling by deep sequencing (CAPP-Seq) as previously described . .. Briefly, cell-free DNA (cfDNA) was extracted from 2 to 4 ml (average of 3.44 ml) of plasma using the QiaAmp Circulating Nucleic Acid Kit (Qiagen, cat. no. 55114) according to manufacturer's instructions.

Multiplex Assay:

Sample Prep:

Article Title: Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pressure
Article Snippet: Paragraph title: Blood sample preparation (Ethics statement) ... To extract cfDNA from a plasma sample, reagents in the QIAamp circulating nucleic acid kit (55114; QIAGEN, Germany) were utilized.

Next-Generation Sequencing:

Article Title: Evaluation of pre-analytical conditions and comparison of the performance of several digital PCR assays for the detection of major EGFR mutations in circulating DNA from non-small cell lung cancers: the CIRCAN_0 study
Article Snippet: These samples were then analyzed using customized ampliseq library and next-generation sequencing (NGS) (PGM, Life Technologies, Carlsbad, CA, USA). .. CfDNA was extracted from 1 mL, 3 mL or 5 mL of plasma using the QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat No 55114, Valencia, CA, USA), with a Qiagen vacuum manifold following the manufacturers' instructions.

Concentration Assay:

Article Title: Radiotherapy increases plasma levels of tumoral cell-free DNA in non-small cell lung cancer patients
Article Snippet: DNA was purified using the QIAamp Circulating Nucleic Acid Kit (55114; Qiagen, Inc., Valencia, CA, USA) according to the manufacturer’s instructions. .. We eluted the DNA in 50 μL double distilled water and stored at –20° C. The DNA concentration was quantified with a Quantus fluorometer (Promega, Madison, WI, USA) following the manufacturer’s instructions.

Article Title: Novel blood-based microRNA biomarker panel for early diagnosis of pancreatic cancer
Article Snippet: MiRNA was extracted from 2.0 to 4.0 mL of plasma using QIAamp circulating Nucleic Acid Kit (Qiagen, Germantown, MD) with QIAvac Connecting System (Qiagen), following manufacturer instructions. .. The concentration of extracted miRNA was determined using Agilent Small RNA kit with 2100 Bioanalyzer (Agilent, Santa Clara, CA), and was in the range of 10 to 100 ng.

DNA Purification:

Article Title: Detection of somatic mutations and HPV in the saliva and plasma of patients with head and neck squamous cell carcinomas
Article Snippet: When frozen tissue was not available, formalin-fixed, paraffin-embedded (FFPE) tissues were used for DNA purification. .. DNA was purified from the white blood cell pellet (normal DNA), saliva, and tumor using an AllPrep Kit (Qiagen, catalog #80204), and from plasma using an QIAamp Circulating Nucleic Acid Kit (Qiagen, catalog #55114).

Lysis:

Article Title: Simple and Low‐Cost Sampling of Cell‐Free Nucleic Acids from Blood Plasma for Rapid and Sensitive Detection of Circulating Tumor DNA
Article Snippet: DTBP could directly capture the nucleic acids through a complex on the surface without the requirement of cell lysis buffer and bulky instruments. .. The DTBP platform was compared with the column‐based cfDNA isolation method (QIAamp Circulating Nucleic Acid Kit, Qiagen, Germany), which was used according to the manufacturer's protocol.