qiaamp circulating nucleic acid kit  (Qiagen)


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    Name:
    QIAamp Circulating Nucleic Acid kit
    Description:

    Catalog Number:
    55114
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    None
    Score:
    85
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    Structured Review

    Qiagen qiaamp circulating nucleic acid kit
    Comparison of extracted <t>cfDNA</t> concentration in qPCR assay. cfDNA concentration in a plasma sample was compared according to extraction methods, <t>QIAamp</t> and PIBEX. cfDNA amount in plasma sample was quantified by qPCR in four reference genes, TERT, RPPH1, GAPDH, and NAGK, in ( a ). The error bar represents standard deviation of triplex qPCR assay in a sample. The relative efficiency of cfDNA extraction in PIBEX was derived based on QIAamp in ( b ). The error bar is standard deviation of samples (n = 7).

    https://www.bioz.com/result/qiaamp circulating nucleic acid kit/product/Qiagen
    Average 99 stars, based on 212 article reviews
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    Images

    1) Product Images from "Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pressure"

    Article Title: Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pressure

    Journal: Scientific Reports

    doi: 10.1038/s41598-018-23766-9

    Comparison of extracted cfDNA concentration in qPCR assay. cfDNA concentration in a plasma sample was compared according to extraction methods, QIAamp and PIBEX. cfDNA amount in plasma sample was quantified by qPCR in four reference genes, TERT, RPPH1, GAPDH, and NAGK, in ( a ). The error bar represents standard deviation of triplex qPCR assay in a sample. The relative efficiency of cfDNA extraction in PIBEX was derived based on QIAamp in ( b ). The error bar is standard deviation of samples (n = 7).
    Figure Legend Snippet: Comparison of extracted cfDNA concentration in qPCR assay. cfDNA concentration in a plasma sample was compared according to extraction methods, QIAamp and PIBEX. cfDNA amount in plasma sample was quantified by qPCR in four reference genes, TERT, RPPH1, GAPDH, and NAGK, in ( a ). The error bar represents standard deviation of triplex qPCR assay in a sample. The relative efficiency of cfDNA extraction in PIBEX was derived based on QIAamp in ( b ). The error bar is standard deviation of samples (n = 7).

    Techniques Used: Concentration Assay, Real-time Polymerase Chain Reaction, Standard Deviation, Derivative Assay

    2) Product Images from "Evaluation of pre-analytical conditions and comparison of the performance of several digital PCR assays for the detection of major EGFR mutations in circulating DNA from non-small cell lung cancers: the CIRCAN_0 study"

    Article Title: Evaluation of pre-analytical conditions and comparison of the performance of several digital PCR assays for the detection of major EGFR mutations in circulating DNA from non-small cell lung cancers: the CIRCAN_0 study

    Journal: Oncotarget

    doi: 10.18632/oncotarget.21256

    Optimization of circulating free DNA (cfDNA) extraction and quantification of cfDNA in the samples (A) Reproducibility of cfDNA extraction using the QIAamp Circulating Acid Kit (Qiagen, Cat No 55114, Valencia, CA, USA) on two independent cfDNA samples extracted from 1 mL (Ai) and 3 mL (Aii) of plasma from NSCLC patients. After extraction, cfDNA was quantified by Qubit dsDNA HS Assay Kit (Life Technologies, Q32854, Carlsbad, CA, USA) according to the manufacturer's instructions. (B) Correlation between the initial volume of plasma 1 mL versus 3 mL (Bi) or 3 mL versus 5 mL (Bii) and the quantity of cfDNA extracted (in ng/μL). (Ci) Fragment size visualization of cfDNA (in bp) from a concentrated (left) and a less concentrated (right) sample obtained using the Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) (Cii) , and average size distribution (10 bp increments) of cfDNA fragments in 77 plasma samples. (D) Correlation between cfDNA concentration measured using the Qubit method and the number of amplifiable copies in the corresponding plasma samples determined using the Quantifiler Kit.
    Figure Legend Snippet: Optimization of circulating free DNA (cfDNA) extraction and quantification of cfDNA in the samples (A) Reproducibility of cfDNA extraction using the QIAamp Circulating Acid Kit (Qiagen, Cat No 55114, Valencia, CA, USA) on two independent cfDNA samples extracted from 1 mL (Ai) and 3 mL (Aii) of plasma from NSCLC patients. After extraction, cfDNA was quantified by Qubit dsDNA HS Assay Kit (Life Technologies, Q32854, Carlsbad, CA, USA) according to the manufacturer's instructions. (B) Correlation between the initial volume of plasma 1 mL versus 3 mL (Bi) or 3 mL versus 5 mL (Bii) and the quantity of cfDNA extracted (in ng/μL). (Ci) Fragment size visualization of cfDNA (in bp) from a concentrated (left) and a less concentrated (right) sample obtained using the Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) (Cii) , and average size distribution (10 bp increments) of cfDNA fragments in 77 plasma samples. (D) Correlation between cfDNA concentration measured using the Qubit method and the number of amplifiable copies in the corresponding plasma samples determined using the Quantifiler Kit.

    Techniques Used: Concentration Assay

    Related Articles

    Centrifugation:

    Article Title: Tracking plasma DNA mutation dynamics in estrogen receptor positive metastatic breast cancer with dPCR-SEQ
    Article Snippet: A QIAamp circulating nucleic acid kit (Qiagen, Valencia, CA, USA; Catalog No. 55114) was used to extract DNA from 2 to 5 ml of plasma according to the manufacturer’s protocol. .. A QIAamp circulating nucleic acid kit (Qiagen, Valencia, CA, USA; Catalog No. 55114) was used to extract DNA from 2 to 5 ml of plasma according to the manufacturer’s protocol.

    Article Title: Noninvasive Digital Detection of Fetal DNA in Plasma of 4-Week-Pregnant Women following In Vitro Fertilization and Embryo Transfer
    Article Snippet: Plasma CNA was extracted using QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat# 55114) by following manufacturer’s protocol. .. Then, 3.6 ml ACB buffer was added to the sample lysate and mixed by pulse-vortexing for 30 seconds, incubated for 5 min on ice and applied to the QIAamp mini column.

    Article Title: Monitoring BRAF and NRAS mutations with cell-free circulating tumor DNA from metastatic melanoma patients
    Article Snippet: For the Idylla assay, DNA was extracted with the specific cartridge and on-board reagents, whereas for Ion Torrent PGM sequencing the DNA was extracted with the Kit QIAamp circulating Nucleic Acid (Qiagen, Hilden, Germany). .. For the Idylla assay, DNA was extracted with the specific cartridge and on-board reagents, whereas for Ion Torrent PGM sequencing the DNA was extracted with the Kit QIAamp circulating Nucleic Acid (Qiagen, Hilden, Germany).

    Article Title: Walking pathways with positive feedback loops reveal DNA methylation biomarkers of colorectal cancer
    Article Snippet: After centrifugation, plasma samples were stored immediately at − 80 °C until cell-free DNA (cfDNA) extraction. .. Cell-free DNA was isolated using the QIAamp Circulating Nucleid Acid (QCNA) Kit (QIAgen, Valencia, CA, USA), as specified by the manufacturer.

    Article Title: An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination
    Article Snippet: RNA was extracted using the QIAamp One-For-All Nucleic Acid Kit on an automated extraction platform (Qiagen BioRobot Universal System, Qiagen, UK) according to the manufacturer's instructions. .. RNA was extracted using the QIAamp One-For-All Nucleic Acid Kit on an automated extraction platform (Qiagen BioRobot Universal System, Qiagen, UK) according to the manufacturer's instructions.

    Amplification:

    Article Title: A comparison of two biological markers of recent hepatitis C virus (HCV) infection: implications for the monitoring of interventions and strategies to reduce HCV transmission among people who inject drugs
    Article Snippet: The entire eluate was extracted on the Qiagen Biorobot MDX platform using the QIAamp One-For-All Nucleic Acid Kit (Qiagen product: 965672) and One For All MDx cV70a protocol. .. The entire eluate was extracted on the Qiagen Biorobot MDX platform using the QIAamp One-For-All Nucleic Acid Kit (Qiagen product: 965672) and One For All MDx cV70a protocol.

    Article Title: Site- and allele-specific polycomb dysregulation in T-cell leukaemia
    Article Snippet: Genomic DNA was extracted using QIAamp deoxyribonucleic acid Blood mini kit according to the manufacturer’s instructions (Qiagen). .. Purified digested DNAs were ligated with an adapter (composed of two complementary primers GWA+ and GWAB− ).

    Article Title: Characterization of Acute HIV-1 Infection in High-Risk Nigerian Populations
    Article Snippet: High-molecular-weight DNA was extracted from at least 2 × 105 PBMCs following the procedures of the QIAamp Nucleic Acid Extraction Assay (Qiagen). .. The proviral DNA was subjected to nearly full-length genome sequencing using HIV-specific primers.

    Quantitative RT-PCR:

    Article Title: An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination
    Article Snippet: RNA was extracted using the QIAamp One-For-All Nucleic Acid Kit on an automated extraction platform (Qiagen BioRobot Universal System, Qiagen, UK) according to the manufacturer's instructions. .. Quantitation of influenza virus in RNA from swabs was performed by analysis of matrix gene transcripts.

    Enzyme-linked Immunosorbent Assay:

    Article Title: TRAIL receptor I (DR4) polymorphisms C626G and A683C are associated with an increased risk for hepatocellular carcinoma (HCC) in HCV-infected patients
    Article Snippet: HCV antibodies were detected with a micro particle enzyme immunoassay (Axsym; Abbott) and confirmed by dot immunoassay (Matrix; Abbott). .. HCV RNA was detected with a nucleic acid purification kit (QIAamp Viral Kit; Qiagen, Hilden, Germany), followed by reverse transcription and nested polymerase chain reaction.

    Microarray:

    Article Title: Deciphering genetic regulation of CD14 by SP1 through characterization of peripheral blood mononuclear transcriptome of P. faiciparum and P. vivax infected malaria patients
    Article Snippet: Samples (n = 35) with total RNA concentration ≥ 25 ng/μl and RIN value 8 were selected for Illumina whole genome microarray and quantitative PCR analyses. .. Genomic DNA was extracted from peripheral blood leukocytes (n = 141) using a QIAamp DNA Blood Kit (Qiagen, Hilden, Germany), quantified spectrophotometrically and stored in −80 °C until further use.

    Incubation:

    Article Title: CAHM, a long non-coding RNA gene hypermethylated in colorectal neoplasia
    Article Snippet: Plasma DNA extraction and bisulphite conversion DNA was extracted from 4 mL plasma using the “QIAamp Circulating Nucleic Acid Kit” (QIAGEN, Cat. 55114). .. Plasma DNA extraction and bisulphite conversion DNA was extracted from 4 mL plasma using the “QIAamp Circulating Nucleic Acid Kit” (QIAGEN, Cat. 55114).

    Article Title: Noninvasive Digital Detection of Fetal DNA in Plasma of 4-Week-Pregnant Women following In Vitro Fertilization and Embryo Transfer
    Article Snippet: Plasma CNA was extracted using QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat# 55114) by following manufacturer’s protocol. .. Plasma CNA was extracted using QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat# 55114) by following manufacturer’s protocol.

    Article Title: An Integrated Next-Generation Sequencing System for Analyzing DNA Mutations, Gene Fusions, and RNA Expression in Lung Cancer
    Article Snippet: For each tissue specimen, multiple sections were scraped from the glass slides and combined into a single tube. .. The tissue was then incubated with xylene to remove residual paraffin followed by extraction using QIAamp® DNA FFPE Tissue Kit (QIAGEN, Germantown, MD) following manufacturer's protocol with the exclusion of RNase treatment in order to recover total nucleic acid (TNA). .. TNA was processed and eluted into 50 μl of ATE buffer (QIAGEN).

    Article Title: Functional diversity and co-operativity between subclonal populations of paediatric glioblastoma and diffuse intrinsic pontine glioma cells
    Article Snippet: Slides were hydrated through an ethanol series prior to manual microdissection into a tube using a sterile fine needle. .. All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the QIAamp DNA FFPE tissue kit protocol (Qiagen, Crawley, UK) using 360μl of Buffer AL, 360μl of ethanol and eluted using 25μl of 10mM Tris buffer at pH8.5 for 7 minutes. .. Matched normal DNA was extracted from blood samples using the DNeasy Blood & Tissue kit (Qiagen, Crawley, UK).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Non-invasive diagnosis of early-stage lung cancer using high-throughput targeted DNA methylation sequencing of circulating tumor DNA (ctDNA)
    Article Snippet: Tissue genomic DNA (gDNA) was isolated from FFPE tissue samples using the Qiagen QIAamp DNA FFPE Tissue Kit (Qiagen, Cat# 56404) according to the manufacture's protocol. gDNA was fragmented to 200 bp using the M220 Focused-ultrasonicator™ (Covaris, Inc.) following the manufacturer's protocol and 100 ng of fragmented DNA was used for library construction. .. For plasma collected using Streck BCT, cfDNA was isolated using the Qiagen QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat# 55114) according to the manufacturer's protocol, while cfDNA was isolated using the Bioo NextPrep-Mag™ cfDNA Isolation Kit (Bioo Scientific, Cat# NOVA-3825-01/3) for plasma collected using EDTA-K2 tubes.

    Article Title: An Integrated Next-Generation Sequencing System for Analyzing DNA Mutations, Gene Fusions, and RNA Expression in Lung Cancer
    Article Snippet: For each tissue specimen, multiple sections were scraped from the glass slides and combined into a single tube. .. The tissue was then incubated with xylene to remove residual paraffin followed by extraction using QIAamp® DNA FFPE Tissue Kit (QIAGEN, Germantown, MD) following manufacturer's protocol with the exclusion of RNase treatment in order to recover total nucleic acid (TNA). .. TNA was processed and eluted into 50 μl of ATE buffer (QIAGEN).

    Article Title: Functional diversity and co-operativity between subclonal populations of paediatric glioblastoma and diffuse intrinsic pontine glioma cells
    Article Snippet: Slides were hydrated through an ethanol series prior to manual microdissection into a tube using a sterile fine needle. .. All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the QIAamp DNA FFPE tissue kit protocol (Qiagen, Crawley, UK) using 360μl of Buffer AL, 360μl of ethanol and eluted using 25μl of 10mM Tris buffer at pH8.5 for 7 minutes. .. Matched normal DNA was extracted from blood samples using the DNeasy Blood & Tissue kit (Qiagen, Crawley, UK).

    Modification:

    Article Title: CAHM, a long non-coding RNA gene hypermethylated in colorectal neoplasia
    Article Snippet: Plasma DNA extraction and bisulphite conversion DNA was extracted from 4 mL plasma using the “QIAamp Circulating Nucleic Acid Kit” (QIAGEN, Cat. 55114). .. A total of ~33 μL DNA was obtained per 4 mL plasma extraction and approximately 31 μL was incubated at 37 °C for 1 h in a final reaction volume of 35 μL containing 2µg/mL tRNA (E. coli ), 280 µg/mL Proteinase K and 1% SDS.

    Article Title: Altered LINE-1 Methylation in Mothers of Children with Down Syndrome
    Article Snippet: Genomic DNA was isolated from frozen blood samples using the QIAamp DNA Blood FlexiGene DNA Kit (Qiagen, Hilden, Germany). .. The MTHFR C677T polymorphism analysis was performed using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) [ ].

    Article Title: Maternal LINE-1 DNA Methylation and Congenital Heart Defects in Down Syndrome
    Article Snippet: Genomic DNA was extracted from peripheral blood leukocytes using the QIAamp DNA Blood FlexiGene DNA Kit (Qiagen, Hilden, Germany). .. The MTHFR C677T polymorphism was evaluated using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) ( ).

    Ligation:

    Article Title: Site- and allele-specific polycomb dysregulation in T-cell leukaemia
    Article Snippet: Paragraph title: Ligation-mediated PCR ... Genomic DNA was extracted using QIAamp deoxyribonucleic acid Blood mini kit according to the manufacturer’s instructions (Qiagen).

    Infection:

    Article Title: TRAIL receptor I (DR4) polymorphisms C626G and A683C are associated with an increased risk for hepatocellular carcinoma (HCC) in HCV-infected patients
    Article Snippet: Paragraph title: Diagnosis of HCV Infection ... HCV RNA was detected with a nucleic acid purification kit (QIAamp Viral Kit; Qiagen, Hilden, Germany), followed by reverse transcription and nested polymerase chain reaction.

    Article Title: A comparison of two biological markers of recent hepatitis C virus (HCV) infection: implications for the monitoring of interventions and strategies to reduce HCV transmission among people who inject drugs
    Article Snippet: The entire eluate was extracted on the Qiagen Biorobot MDX platform using the QIAamp One-For-All Nucleic Acid Kit (Qiagen product: 965672) and One For All MDx cV70a protocol. .. The entire eluate was extracted on the Qiagen Biorobot MDX platform using the QIAamp One-For-All Nucleic Acid Kit (Qiagen product: 965672) and One For All MDx cV70a protocol.

    other:

    Article Title: Increased Plasma Circulating Cell-Free DNA Could Be a Potential Marker for Oral Cancer
    Article Snippet: The separation and storage of the plasma samples was performed within 3 h of blood collection at 4 °C. cfDNA was extracted from 1 mL plasma aliquots using the QIAamp Circulating Nucleic Acid Kit (QIAamp-Blood Mini Kit, QIAGEN, Chatsworth, CA, USA) following the manufacturer’s recommendations and was eluted in 60 μL elution buffer from the kit.

    Sequencing:

    Article Title: Monitoring BRAF and NRAS mutations with cell-free circulating tumor DNA from metastatic melanoma patients
    Article Snippet: The plasma was obtained by two rounds of centrifugation (10 minutes, 2000 g at 4°C) aliquoted into 1mL tubes and stored at −80°C until use. .. For the Idylla assay, DNA was extracted with the specific cartridge and on-board reagents, whereas for Ion Torrent PGM sequencing the DNA was extracted with the Kit QIAamp circulating Nucleic Acid (Qiagen, Hilden, Germany). .. The cell free DNA concentration was measured with the Qubit Fluorometric Quantitation (ThermoFisher Scientific) double strand (ds) DNA assay kit.

    Article Title: Walking pathways with positive feedback loops reveal DNA methylation biomarkers of colorectal cancer
    Article Snippet: Cell-free DNA was isolated using the QIAamp Circulating Nucleid Acid (QCNA) Kit (QIAgen, Valencia, CA, USA), as specified by the manufacturer. .. Methylation of 47 CpG-locus was measured by Pyrosequencing.

    Article Title: Characterization of Acute HIV-1 Infection in High-Risk Nigerian Populations
    Article Snippet: Paragraph title: HIV-1 Sequencing ... High-molecular-weight DNA was extracted from at least 2 × 105 PBMCs following the procedures of the QIAamp Nucleic Acid Extraction Assay (Qiagen).

    Article Title: Functional diversity and co-operativity between subclonal populations of paediatric glioblastoma and diffuse intrinsic pontine glioma cells
    Article Snippet: All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the QIAamp DNA FFPE tissue kit protocol (Qiagen, Crawley, UK) using 360μl of Buffer AL, 360μl of ethanol and eluted using 25μl of 10mM Tris buffer at pH8.5 for 7 minutes. .. All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the QIAamp DNA FFPE tissue kit protocol (Qiagen, Crawley, UK) using 360μl of Buffer AL, 360μl of ethanol and eluted using 25μl of 10mM Tris buffer at pH8.5 for 7 minutes.

    Nucleic Acid Purification:

    Article Title: TRAIL receptor I (DR4) polymorphisms C626G and A683C are associated with an increased risk for hepatocellular carcinoma (HCC) in HCV-infected patients
    Article Snippet: HCV antibodies were detected with a micro particle enzyme immunoassay (Axsym; Abbott) and confirmed by dot immunoassay (Matrix; Abbott). .. HCV RNA was detected with a nucleic acid purification kit (QIAamp Viral Kit; Qiagen, Hilden, Germany), followed by reverse transcription and nested polymerase chain reaction. .. Quantitative determination of HCV loads was done by branched DNA technology (Chiron, Emeryville, CA).

    DNA Extraction:

    Article Title: CAHM, a long non-coding RNA gene hypermethylated in colorectal neoplasia
    Article Snippet: Enzymatically methylated human DNA (CpGenome Universal Methylated DNA, Millipore, Cat. S7821) was used as a positive methylation control, and white blood cell DNA (WBC DNA, Human Genomic DNA from human buffy coat, Roche Applied Science, Cat. 1169111201) as an unmethylated control. .. Plasma DNA extraction and bisulphite conversion DNA was extracted from 4 mL plasma using the “QIAamp Circulating Nucleic Acid Kit” (QIAGEN, Cat. 55114). .. The manufacturer’s protocol was followed except that the column was washed twice with ACW2 and twice with 100% ethanol, after which DNA was eluted by applying 35 μL of AVE buffer twice over the column.

    Article Title: Noninvasive Digital Detection of Fetal DNA in Plasma of 4-Week-Pregnant Women following In Vitro Fertilization and Embryo Transfer
    Article Snippet: Paragraph title: Plasma DNA Extraction ... Plasma CNA was extracted using QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat# 55114) by following manufacturer’s protocol.

    Article Title: Analysis of solid tumor mutation profiles in liquid biopsy, et al. Analysis of solid tumor mutation profiles in liquid biopsy
    Article Snippet: Paragraph title: Cell‐free DNA extraction from blood ... Total cfDNA was extracted from plasma using QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat. No: 55114), as per standard protocol.

    Article Title: Characterization of Acute HIV-1 Infection in High-Risk Nigerian Populations
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were purified from the rest of blood components by the Ficoll Hypaque procedure, and the PBMCs were used for DNA extraction. .. High-molecular-weight DNA was extracted from at least 2 × 105 PBMCs following the procedures of the QIAamp Nucleic Acid Extraction Assay (Qiagen).

    Methylation:

    Article Title: CAHM, a long non-coding RNA gene hypermethylated in colorectal neoplasia
    Article Snippet: Plasma DNA extraction and bisulphite conversion DNA was extracted from 4 mL plasma using the “QIAamp Circulating Nucleic Acid Kit” (QIAGEN, Cat. 55114). .. A total of ~33 μL DNA was obtained per 4 mL plasma extraction and approximately 31 μL was incubated at 37 °C for 1 h in a final reaction volume of 35 μL containing 2µg/mL tRNA (E. coli ), 280 µg/mL Proteinase K and 1% SDS.

    Article Title: Walking pathways with positive feedback loops reveal DNA methylation biomarkers of colorectal cancer
    Article Snippet: Cell-free DNA was isolated using the QIAamp Circulating Nucleid Acid (QCNA) Kit (QIAgen, Valencia, CA, USA), as specified by the manufacturer. .. Bisulfite conversion of cfDNA was performed using the EZ DNA Methylation-Gold Kit.

    Isolation:

    Article Title: Analysis of solid tumor mutation profiles in liquid biopsy, et al. Analysis of solid tumor mutation profiles in liquid biopsy
    Article Snippet: Total cfDNA was extracted from plasma using QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat. No: 55114), as per standard protocol. .. Total cfDNA was extracted from plasma using QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat. No: 55114), as per standard protocol.

    Article Title: Non-invasive diagnosis of early-stage lung cancer using high-throughput targeted DNA methylation sequencing of circulating tumor DNA (ctDNA)
    Article Snippet: Tissue genomic DNA (gDNA) was isolated from FFPE tissue samples using the Qiagen QIAamp DNA FFPE Tissue Kit (Qiagen, Cat# 56404) according to the manufacture's protocol. gDNA was fragmented to 200 bp using the M220 Focused-ultrasonicator™ (Covaris, Inc.) following the manufacturer's protocol and 100 ng of fragmented DNA was used for library construction. .. For plasma collected using Streck BCT, cfDNA was isolated using the Qiagen QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat# 55114) according to the manufacturer's protocol, while cfDNA was isolated using the Bioo NextPrep-Mag™ cfDNA Isolation Kit (Bioo Scientific, Cat# NOVA-3825-01/3) for plasma collected using EDTA-K2 tubes. .. Repeated freezing and thawing of plasma was avoided to prevent cfDNA degradation and gDNA contamination from white blood cells (WBC).

    Article Title: Walking pathways with positive feedback loops reveal DNA methylation biomarkers of colorectal cancer
    Article Snippet: After centrifugation, plasma samples were stored immediately at − 80 °C until cell-free DNA (cfDNA) extraction. .. Cell-free DNA was isolated using the QIAamp Circulating Nucleid Acid (QCNA) Kit (QIAgen, Valencia, CA, USA), as specified by the manufacturer. .. Bisulfite conversion of cfDNA was performed using the EZ DNA Methylation-Gold Kit.

    Article Title: An Integrated Next-Generation Sequencing System for Analyzing DNA Mutations, Gene Fusions, and RNA Expression in Lung Cancer
    Article Snippet: The tissue was then incubated with xylene to remove residual paraffin followed by extraction using QIAamp® DNA FFPE Tissue Kit (QIAGEN, Germantown, MD) following manufacturer's protocol with the exclusion of RNase treatment in order to recover total nucleic acid (TNA). .. The tissue was then incubated with xylene to remove residual paraffin followed by extraction using QIAamp® DNA FFPE Tissue Kit (QIAGEN, Germantown, MD) following manufacturer's protocol with the exclusion of RNase treatment in order to recover total nucleic acid (TNA).

    Article Title: Deciphering genetic regulation of CD14 by SP1 through characterization of peripheral blood mononuclear transcriptome of P. faiciparum and P. vivax infected malaria patients
    Article Snippet: Total RNA was isolated following manufacturer's protocol using RNeasy® Plus Minikit (QIAGEN) and quantified using nanodrop 2000C spectrophotometer (Thermo Scientific). .. Genomic DNA was extracted from peripheral blood leukocytes (n = 141) using a QIAamp DNA Blood Kit (Qiagen, Hilden, Germany), quantified spectrophotometrically and stored in −80 °C until further use.

    Article Title: Altered LINE-1 Methylation in Mothers of Children with Down Syndrome
    Article Snippet: The questionnaire was adapted from a food frequency questionnaire validated for the Croatian women [ ]. .. Genomic DNA was isolated from frozen blood samples using the QIAamp DNA Blood FlexiGene DNA Kit (Qiagen, Hilden, Germany). .. The parental origin of trisomy 21 was determined as previously described [ ].

    Purification:

    Article Title: The molecular evolution of acquired resistance to targeted EGFR blockade in colorectal cancers
    Article Snippet: KRAS mutational status in the tissue was predetermined using the DxS assay (Qiagen). .. DNA was purified from of 0.2–1mL of banked serum using the QIAamp circulating nucleic acid kit (Qiagen, cat. # 55114) following the manufacturer’s recommendations. .. Amplifiable DNA was quantified with qPCR, employing primers and conditions as previously described .

    Article Title: Genome-Wide Plasma Cell-Free DNA Methylation Profiling Identifies Potential Biomarkers for Lung Cancer
    Article Snippet: All blood samples from the control group and lung cancer patients (∼5 ml) were collected in tubes containing EDTA as anticoagulant, centrifuged for 15 min at 1500 × g. The purified plasma was then stored at -80°C. .. The cfDNA was extracted from plasma using QIAamp Circulating Nucleic Acid kit (Qiagen, 55114) according to manufacturer's protocol.

    Article Title: Site- and allele-specific polycomb dysregulation in T-cell leukaemia
    Article Snippet: Genomic DNA was extracted using QIAamp deoxyribonucleic acid Blood mini kit according to the manufacturer’s instructions (Qiagen). .. DNA (500 ng) was used for LM-PCR; DNA was digested by DraI, EcoRV, PvuII, SmaI, SspI or StuI restriction endonucleases.

    Article Title: Characterization of Acute HIV-1 Infection in High-Risk Nigerian Populations
    Article Snippet: Peripheral blood mononuclear cells (PBMCs) were purified from the rest of blood components by the Ficoll Hypaque procedure, and the PBMCs were used for DNA extraction. .. High-molecular-weight DNA was extracted from at least 2 × 105 PBMCs following the procedures of the QIAamp Nucleic Acid Extraction Assay (Qiagen).

    Polymerase Chain Reaction:

    Article Title: Walking pathways with positive feedback loops reveal DNA methylation biomarkers of colorectal cancer
    Article Snippet: Cell-free DNA was isolated using the QIAamp Circulating Nucleid Acid (QCNA) Kit (QIAgen, Valencia, CA, USA), as specified by the manufacturer. .. Methylation of 47 CpG-locus was measured by Pyrosequencing.

    Article Title: An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination
    Article Snippet: RNA was extracted using the QIAamp One-For-All Nucleic Acid Kit on an automated extraction platform (Qiagen BioRobot Universal System, Qiagen, UK) according to the manufacturer's instructions. .. Quantitation of influenza virus in RNA from swabs was performed by analysis of matrix gene transcripts.

    Article Title: A comparison of two biological markers of recent hepatitis C virus (HCV) infection: implications for the monitoring of interventions and strategies to reduce HCV transmission among people who inject drugs
    Article Snippet: The entire eluate was extracted on the Qiagen Biorobot MDX platform using the QIAamp One-For-All Nucleic Acid Kit (Qiagen product: 965672) and One For All MDx cV70a protocol. .. The entire eluate was extracted on the Qiagen Biorobot MDX platform using the QIAamp One-For-All Nucleic Acid Kit (Qiagen product: 965672) and One For All MDx cV70a protocol.

    Article Title: Site- and allele-specific polycomb dysregulation in T-cell leukaemia
    Article Snippet: Paragraph title: Ligation-mediated PCR ... Genomic DNA was extracted using QIAamp deoxyribonucleic acid Blood mini kit according to the manufacturer’s instructions (Qiagen).

    Article Title: Characterization of Acute HIV-1 Infection in High-Risk Nigerian Populations
    Article Snippet: High-molecular-weight DNA was extracted from at least 2 × 105 PBMCs following the procedures of the QIAamp Nucleic Acid Extraction Assay (Qiagen). .. The proviral DNA was subjected to nearly full-length genome sequencing using HIV-specific primers.

    Article Title: Altered LINE-1 Methylation in Mothers of Children with Down Syndrome
    Article Snippet: Genomic DNA was isolated from frozen blood samples using the QIAamp DNA Blood FlexiGene DNA Kit (Qiagen, Hilden, Germany). .. Genomic DNA was quantified using a spectrophotometer according to the manufacturer's instructions (BioMateTM3, Thermo Electron Corporation, USA).

    Article Title: Maternal LINE-1 DNA Methylation and Congenital Heart Defects in Down Syndrome
    Article Snippet: Genomic DNA was extracted from peripheral blood leukocytes using the QIAamp DNA Blood FlexiGene DNA Kit (Qiagen, Hilden, Germany). .. Genomic DNA was extracted from peripheral blood leukocytes using the QIAamp DNA Blood FlexiGene DNA Kit (Qiagen, Hilden, Germany).

    Nested PCR:

    Article Title: TRAIL receptor I (DR4) polymorphisms C626G and A683C are associated with an increased risk for hepatocellular carcinoma (HCC) in HCV-infected patients
    Article Snippet: HCV antibodies were detected with a micro particle enzyme immunoassay (Axsym; Abbott) and confirmed by dot immunoassay (Matrix; Abbott). .. HCV RNA was detected with a nucleic acid purification kit (QIAamp Viral Kit; Qiagen, Hilden, Germany), followed by reverse transcription and nested polymerase chain reaction. .. Quantitative determination of HCV loads was done by branched DNA technology (Chiron, Emeryville, CA).

    Sample Prep:

    Article Title: Walking pathways with positive feedback loops reveal DNA methylation biomarkers of colorectal cancer
    Article Snippet: Paragraph title: Sample preparation protocol ... Cell-free DNA was isolated using the QIAamp Circulating Nucleid Acid (QCNA) Kit (QIAgen, Valencia, CA, USA), as specified by the manufacturer.

    Real-time Polymerase Chain Reaction:

    Article Title: An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination
    Article Snippet: RNA was extracted using the QIAamp One-For-All Nucleic Acid Kit on an automated extraction platform (Qiagen BioRobot Universal System, Qiagen, UK) according to the manufacturer's instructions. .. RNA was extracted using the QIAamp One-For-All Nucleic Acid Kit on an automated extraction platform (Qiagen BioRobot Universal System, Qiagen, UK) according to the manufacturer's instructions.

    Article Title: Deciphering genetic regulation of CD14 by SP1 through characterization of peripheral blood mononuclear transcriptome of P. faiciparum and P. vivax infected malaria patients
    Article Snippet: Samples (n = 35) with total RNA concentration ≥ 25 ng/μl and RIN value 8 were selected for Illumina whole genome microarray and quantitative PCR analyses. .. Genomic DNA was extracted from peripheral blood leukocytes (n = 141) using a QIAamp DNA Blood Kit (Qiagen, Hilden, Germany), quantified spectrophotometrically and stored in −80 °C until further use.

    Agarose Gel Electrophoresis:

    Article Title: Site- and allele-specific polycomb dysregulation in T-cell leukaemia
    Article Snippet: Genomic DNA was extracted using QIAamp deoxyribonucleic acid Blood mini kit according to the manufacturer’s instructions (Qiagen). .. A primary PCR amplification was performed using an adapter-specific primer (AP1) and primer specific for the different TCRβ gene segments (ext).

    Homogenization:

    Article Title: Functional diversity and co-operativity between subclonal populations of paediatric glioblastoma and diffuse intrinsic pontine glioma cells
    Article Snippet: DNA was extracted from frozen tissue by homogenisation prior to following the DNeasy Blood & Tissue kit protocol (Qiagen, Crawley, UK). .. All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the QIAamp DNA FFPE tissue kit protocol (Qiagen, Crawley, UK) using 360μl of Buffer AL, 360μl of ethanol and eluted using 25μl of 10mM Tris buffer at pH8.5 for 7 minutes.

    Laser Capture Microdissection:

    Article Title: Functional diversity and co-operativity between subclonal populations of paediatric glioblastoma and diffuse intrinsic pontine glioma cells
    Article Snippet: All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the QIAamp DNA FFPE tissue kit protocol (Qiagen, Crawley, UK) using 360μl of Buffer AL, 360μl of ethanol and eluted using 25μl of 10mM Tris buffer at pH8.5 for 7 minutes. .. All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the QIAamp DNA FFPE tissue kit protocol (Qiagen, Crawley, UK) using 360μl of Buffer AL, 360μl of ethanol and eluted using 25μl of 10mM Tris buffer at pH8.5 for 7 minutes.

    Spectrophotometry:

    Article Title: Deciphering genetic regulation of CD14 by SP1 through characterization of peripheral blood mononuclear transcriptome of P. faiciparum and P. vivax infected malaria patients
    Article Snippet: Total RNA was isolated following manufacturer's protocol using RNeasy® Plus Minikit (QIAGEN) and quantified using nanodrop 2000C spectrophotometer (Thermo Scientific). .. Genomic DNA was extracted from peripheral blood leukocytes (n = 141) using a QIAamp DNA Blood Kit (Qiagen, Hilden, Germany), quantified spectrophotometrically and stored in −80 °C until further use.

    Article Title: Altered LINE-1 Methylation in Mothers of Children with Down Syndrome
    Article Snippet: Genomic DNA was isolated from frozen blood samples using the QIAamp DNA Blood FlexiGene DNA Kit (Qiagen, Hilden, Germany). .. Genomic DNA was isolated from frozen blood samples using the QIAamp DNA Blood FlexiGene DNA Kit (Qiagen, Hilden, Germany).

    Sampling:

    Article Title: Functional diversity and co-operativity between subclonal populations of paediatric glioblastoma and diffuse intrinsic pontine glioma cells
    Article Snippet: We obtained longitudinal paired samples from two patients from the Centre Hospitalier Régional et Universitaire Hautepierre, Strasbourg, France; five DIPG patients with multiple sampling taken at autopsy from the Hospital San Joan de Deu, Barcelona, Spain; five DIPG patients with multiple sampling taken at autopsy from Stanford Medical School, Stanford, CA, USA; three cases with multiple samples from the Queensland Children’s Tumour Bank, Brisbane, Australia; one case each with multiple samples from St Georges Hospital and Kings College Hospital, London, UK , all of which were collected locally after informed consent. .. All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the QIAamp DNA FFPE tissue kit protocol (Qiagen, Crawley, UK) using 360μl of Buffer AL, 360μl of ethanol and eluted using 25μl of 10mM Tris buffer at pH8.5 for 7 minutes.

    Concentration Assay:

    Article Title: Non-invasive diagnosis of early-stage lung cancer using high-throughput targeted DNA methylation sequencing of circulating tumor DNA (ctDNA)
    Article Snippet: For plasma collected using Streck BCT, cfDNA was isolated using the Qiagen QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat# 55114) according to the manufacturer's protocol, while cfDNA was isolated using the Bioo NextPrep-Mag™ cfDNA Isolation Kit (Bioo Scientific, Cat# NOVA-3825-01/3) for plasma collected using EDTA-K2 tubes. .. For plasma collected using Streck BCT, cfDNA was isolated using the Qiagen QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat# 55114) according to the manufacturer's protocol, while cfDNA was isolated using the Bioo NextPrep-Mag™ cfDNA Isolation Kit (Bioo Scientific, Cat# NOVA-3825-01/3) for plasma collected using EDTA-K2 tubes.

    Article Title: Deciphering genetic regulation of CD14 by SP1 through characterization of peripheral blood mononuclear transcriptome of P. faiciparum and P. vivax infected malaria patients
    Article Snippet: Samples (n = 35) with total RNA concentration ≥ 25 ng/μl and RIN value 8 were selected for Illumina whole genome microarray and quantitative PCR analyses. .. Genomic DNA was extracted from peripheral blood leukocytes (n = 141) using a QIAamp DNA Blood Kit (Qiagen, Hilden, Germany), quantified spectrophotometrically and stored in −80 °C until further use.

    DNA Purification:

    Article Title: The molecular evolution of acquired resistance to targeted EGFR blockade in colorectal cancers
    Article Snippet: Paragraph title: DNA purification ... DNA was purified from of 0.2–1mL of banked serum using the QIAamp circulating nucleic acid kit (Qiagen, cat. # 55114) following the manufacturer’s recommendations.

    Lysis:

    Article Title: A comparison of two biological markers of recent hepatitis C virus (HCV) infection: implications for the monitoring of interventions and strategies to reduce HCV transmission among people who inject drugs
    Article Snippet: RNA testing involved elution from the DBS by incubating the 6 mm spot for 2 hours at 56 °C with 20 µL of proteinase K and 300 µL of ATL lysis buffer (Qiagen products: 19133 and 19076). .. The entire eluate was extracted on the Qiagen Biorobot MDX platform using the QIAamp One-For-All Nucleic Acid Kit (Qiagen product: 965672) and One For All MDx cV70a protocol.

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    Qiagen qiaamp circulating nucleic acid kit
    Qiaamp Circulating Nucleic Acid Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiaamp circulating nucleic acid kit/product/Qiagen
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    qiaamp circulating nucleic acid kit - by Bioz Stars, 2019-12
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