qiaamp  (Qiagen)


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    Name:
    QIAamp Circulating Nucleic Acid Kit
    Description:
    For isolation of free circulating DNA and RNA from human plasma or serum Kit contents Qiagen QIAamp Circulating Nucleic Acid Kit 50 preps 1 to 5mL Sample 20 to 150L Elution Volume Serum Plasma Sample Free circulating DNA RNA and miRNA Viral DNA Viral RNA Purification QIAamp Mini Column Format Silica Technology For Isolation of Free circulating DNA and RNA from Human Plasma or Serum Includes QIAamp Mini Columns 20mL Tube Extenders Qiagen Proteinase K Carrier RNA Buffers VacConnectors and 1 5mL and 2mL Collection Tubes Benefits Concentration of nucleic acids with high input and low elution volumes Efficient recovery of fragmented DNA and RNA No organic extraction or ethanol precipitation Removal of contaminants and inhibitors
    Catalog Number:
    55114
    Price:
    1091
    Category:
    QIAamp Circulating Nucleic Acid Kit
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    Structured Review

    Qiagen qiaamp
    QIAamp Circulating Nucleic Acid Kit
    For isolation of free circulating DNA and RNA from human plasma or serum Kit contents Qiagen QIAamp Circulating Nucleic Acid Kit 50 preps 1 to 5mL Sample 20 to 150L Elution Volume Serum Plasma Sample Free circulating DNA RNA and miRNA Viral DNA Viral RNA Purification QIAamp Mini Column Format Silica Technology For Isolation of Free circulating DNA and RNA from Human Plasma or Serum Includes QIAamp Mini Columns 20mL Tube Extenders Qiagen Proteinase K Carrier RNA Buffers VacConnectors and 1 5mL and 2mL Collection Tubes Benefits Concentration of nucleic acids with high input and low elution volumes Efficient recovery of fragmented DNA and RNA No organic extraction or ethanol precipitation Removal of contaminants and inhibitors
    https://www.bioz.com/result/qiaamp/product/Qiagen
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiaamp - by Bioz Stars, 2021-03
    97/100 stars

    Images

    1) Product Images from "Evaluation of pre-analytical conditions and comparison of the performance of several digital PCR assays for the detection of major EGFR mutations in circulating DNA from non-small cell lung cancers: the CIRCAN_0 study"

    Article Title: Evaluation of pre-analytical conditions and comparison of the performance of several digital PCR assays for the detection of major EGFR mutations in circulating DNA from non-small cell lung cancers: the CIRCAN_0 study

    Journal: Oncotarget

    doi: 10.18632/oncotarget.21256

    Optimization of circulating free DNA (cfDNA) extraction and quantification of cfDNA in the samples (A) Reproducibility of cfDNA extraction using the QIAamp Circulating Acid Kit (Qiagen, Cat No 55114, Valencia, CA, USA) on two independent cfDNA samples extracted from 1 mL (Ai) and 3 mL (Aii) of plasma from NSCLC patients. After extraction, cfDNA was quantified by Qubit dsDNA HS Assay Kit (Life Technologies, Q32854, Carlsbad, CA, USA) according to the manufacturer's instructions. (B) Correlation between the initial volume of plasma 1 mL versus 3 mL (Bi) or 3 mL versus 5 mL (Bii) and the quantity of cfDNA extracted (in ng/μL). (Ci) Fragment size visualization of cfDNA (in bp) from a concentrated (left) and a less concentrated (right) sample obtained using the Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) (Cii) , and average size distribution (10 bp increments) of cfDNA fragments in 77 plasma samples. (D) Correlation between cfDNA concentration measured using the Qubit method and the number of amplifiable copies in the corresponding plasma samples determined using the Quantifiler Kit.
    Figure Legend Snippet: Optimization of circulating free DNA (cfDNA) extraction and quantification of cfDNA in the samples (A) Reproducibility of cfDNA extraction using the QIAamp Circulating Acid Kit (Qiagen, Cat No 55114, Valencia, CA, USA) on two independent cfDNA samples extracted from 1 mL (Ai) and 3 mL (Aii) of plasma from NSCLC patients. After extraction, cfDNA was quantified by Qubit dsDNA HS Assay Kit (Life Technologies, Q32854, Carlsbad, CA, USA) according to the manufacturer's instructions. (B) Correlation between the initial volume of plasma 1 mL versus 3 mL (Bi) or 3 mL versus 5 mL (Bii) and the quantity of cfDNA extracted (in ng/μL). (Ci) Fragment size visualization of cfDNA (in bp) from a concentrated (left) and a less concentrated (right) sample obtained using the Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) (Cii) , and average size distribution (10 bp increments) of cfDNA fragments in 77 plasma samples. (D) Correlation between cfDNA concentration measured using the Qubit method and the number of amplifiable copies in the corresponding plasma samples determined using the Quantifiler Kit.

    Techniques Used: Concentration Assay

    2) Product Images from "Simultaneous Isolation of Circulating Nucleic Acids and EV-Associated Protein Biomarkers From Unprocessed Plasma Using an AC Electrokinetics-Based Platform"

    Article Title: Simultaneous Isolation of Circulating Nucleic Acids and EV-Associated Protein Biomarkers From Unprocessed Plasma Using an AC Electrokinetics-Based Platform

    Journal: Frontiers in Bioengineering and Biotechnology

    doi: 10.3389/fbioe.2020.581157

    ACE-captured and isolated DNA can be amplified using PCR and NGS. (A) qPCR amplification profiles for the KRAS G12D mutation and ACTB housekeeping gene using DNA isolated from AsPC-1 cell culture EVs using either the ACE chip or the QIAamp Circulating Nucleic Acid Kit (QNA). (B) Variant allele frequency detected in the HD701 quantitative multiplex reference DNA standard after ACE isolation, compared to the expected frequency. (C) Variant allele frequency shown for FBXW7 , KRAS , and TP53 mutations in DNA captured from AsPC-1 cell culture EVs. (D) Variant allele frequency shown for TP53 and KRAS point mutations in DNA captured from a lung cancer donor plasma sample (Donor 5734). Each replicate indicates an isolation from one chip and the subsequent creation of one NGS library.
    Figure Legend Snippet: ACE-captured and isolated DNA can be amplified using PCR and NGS. (A) qPCR amplification profiles for the KRAS G12D mutation and ACTB housekeeping gene using DNA isolated from AsPC-1 cell culture EVs using either the ACE chip or the QIAamp Circulating Nucleic Acid Kit (QNA). (B) Variant allele frequency detected in the HD701 quantitative multiplex reference DNA standard after ACE isolation, compared to the expected frequency. (C) Variant allele frequency shown for FBXW7 , KRAS , and TP53 mutations in DNA captured from AsPC-1 cell culture EVs. (D) Variant allele frequency shown for TP53 and KRAS point mutations in DNA captured from a lung cancer donor plasma sample (Donor 5734). Each replicate indicates an isolation from one chip and the subsequent creation of one NGS library.

    Techniques Used: Isolation, Amplification, Polymerase Chain Reaction, Next-Generation Sequencing, Real-time Polymerase Chain Reaction, Mutagenesis, Cell Culture, Chromatin Immunoprecipitation, Variant Assay, Multiplex Assay

    3) Product Images from "Estimating the rate of cell type degeneration from epigenetic sequencing of cell-free DNA"

    Article Title: Estimating the rate of cell type degeneration from epigenetic sequencing of cell-free DNA

    Journal: bioRxiv

    doi: 10.1101/2020.01.15.907022

    (A) CfDNA concentrations for 28 cases and 25 controls (B) CelFiE decomposition estimates for 16 ALS patients (light blue) and 16 controls (dark blue).
    Figure Legend Snippet: (A) CfDNA concentrations for 28 cases and 25 controls (B) CelFiE decomposition estimates for 16 ALS patients (light blue) and 16 controls (dark blue).

    Techniques Used:

    4) Product Images from "Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification"

    Article Title: Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification

    Journal: Analytical and Bioanalytical Chemistry

    doi: 10.1007/s00216-014-7835-3

    Assessment of cell-free DNA (cfDNA) yield using four extraction methods. The mean yield ± one standard deviation from replicate extractions using plasma pool A (i) (with or without ADH ) performed with the QIAamp circulating nucleic acid ( CNA ), NucleoSpin Plasma XS ( NS ) and FitAmp plasma/serum DNA isolation ( FA ) kits ( n = 10) and the QIAamp DNA blood mini ( DBM ) kit ( n = 9) is displayed relative to the mean yield of the DBM kit. The yield of cfDNA was quantified by quantitative PCR (qPCR) assays to TERT and ALUJ
    Figure Legend Snippet: Assessment of cell-free DNA (cfDNA) yield using four extraction methods. The mean yield ± one standard deviation from replicate extractions using plasma pool A (i) (with or without ADH ) performed with the QIAamp circulating nucleic acid ( CNA ), NucleoSpin Plasma XS ( NS ) and FitAmp plasma/serum DNA isolation ( FA ) kits ( n = 10) and the QIAamp DNA blood mini ( DBM ) kit ( n = 9) is displayed relative to the mean yield of the DBM kit. The yield of cfDNA was quantified by quantitative PCR (qPCR) assays to TERT and ALUJ

    Techniques Used: Standard Deviation, DNA Extraction, Real-time Polymerase Chain Reaction

    5) Product Images from "Detection of somatic mutations in cell-free DNA in plasma and correlation with overall survival in patients with solid tumors"

    Article Title: Detection of somatic mutations in cell-free DNA in plasma and correlation with overall survival in patients with solid tumors

    Journal: Oncotarget

    doi: 10.18632/oncotarget.21982

    Average cfDNA yield (ng/mL) in different solid tumor types studied cfDNA extracted using the QIAamp Circulating Nucleic Acid Kit and quantified using the Qubit dsDNA HS Assay cfDNA yield (ng/mL). n represent the number tested for each tumor type. * one sample excluded from graph due to high cfDNA concentration (769 ng/mL).
    Figure Legend Snippet: Average cfDNA yield (ng/mL) in different solid tumor types studied cfDNA extracted using the QIAamp Circulating Nucleic Acid Kit and quantified using the Qubit dsDNA HS Assay cfDNA yield (ng/mL). n represent the number tested for each tumor type. * one sample excluded from graph due to high cfDNA concentration (769 ng/mL).

    Techniques Used: Concentration Assay

    Related Articles

    Isolation:

    Article Title: A Versatile Nanowire Platform for Highly Efficient Isolation and Direct PCR-free Colorimetric Detection of Human Papillomavirus DNA from Unprocessed Urine
    Article Snippet: Paired urine samples were applied to PEI-mPpy NWs and Qiagen reagents to efficiently isolate HPV DNA and elucidate their genotype profiles by qPCR. .. Of the 15 HPV-positive samples, HPV DNA was identified in all 15 (100%) when isolated by using PEI-mPpy NWs, whereas HPV DNA was detected in 5 urine samples (33.3%) when extracted with the Qiagen kit. .. Indeed, HPV DNA recovered by the PEI-mPpy NWs exhibited obviously lower cycle threshold (Ct) values compared to that from the Qiagen kit, which strongly suggests higher performance of the nanowires in DNA recovery yield and integrity.

    Article Title: Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells
    Article Snippet: .. B: From serum (300 μL) samples, no cut-off. (PDF 1 MB) Additional file 3: Figure S3: Assessment of bias in RNA isolation from PBMCs samples using the Qiagen kit, comparison of extraction from 3×106 and 1×106 cells with the same amount of RNA for RT (110 ng). ..

    Article Title: Magnetic Nanowire Networks for Dual-Isolation and Detection of Tumor-Associated Circulating Biomarkers
    Article Snippet: .. A relatively low sensitivity for mutations was detected in the cfDNA isolated using the Qiagen kit. .. Using the patient's blood, we investigated the relationship between the number of NW networks and cfDNA concentration (Figure c).

    Quantitative RT-PCR:

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification
    Article Snippet: .. The qRT-PCR analysis indicated no significant difference in amplifications between RNA purified by the Qiagen kit and the RNA purified using a filter paper-based spin column with homemade buffers. .. The Cq value of the reactions using the same amount of starting RNAs from these methods was similar (28.29 ± 0.09 and 28.61 ± 0.13) with the similar cross point of their amplification curves with a threshold ( ).

    Purification:

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification
    Article Snippet: .. The qRT-PCR analysis indicated no significant difference in amplifications between RNA purified by the Qiagen kit and the RNA purified using a filter paper-based spin column with homemade buffers. .. The Cq value of the reactions using the same amount of starting RNAs from these methods was similar (28.29 ± 0.09 and 28.61 ± 0.13) with the similar cross point of their amplification curves with a threshold ( ).

    Concentration Assay:

    Article Title: METHOD FOR MICRORNA ISOLATION FROM CLINICAL SERUM SAMPLES
    Article Snippet: As RNA was eluted in the same volume of solution in using all three kits, the concentration of microRNA would directly indicate the microRNA yield. .. While the concentration of microRNA from the Qiagen kit was 48.8 pg/μL, the concentrations of microRNA from the Ambion kit and the Norgen kit were 29.3 pg/μL and 11.7 pg/μL, respectively. ..

    Amplification:

    Article Title: Efficient Capture and Isolation of Tumor-Related Circulating Cell-Free DNA from Cancer Patients Using Electroactive Conducting Polymer Nanowire Platforms
    Article Snippet: Our results showed that most of the cfDNAs extracted using Ppy/Au NWs and the Qiagen kit were closely related to specific PCR bands corresponding to the EGFR, KRAS, and P53 genes. .. However, in the case of samples B7 and B8, the KRAS, EGFR, and P53 genes were not amplified from the cfDNAs collected using the Qiagen kit, but all three genes were clearly amplified when cfDNAs were extracted using Ppy/Au NWs. .. Because the levels of eluted cfDNA can strongly influence the detection and quantification of certain target genes, we explored the feasibility of analysing EGFR mutations in the recovered cfDNAs using droplet digital PCR (ddPCR).

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    • qiagen kit  (Qiagen)
    97
    Qiagen qiagen kit
    NW networks as a highly efficient platform for isolation and analysis of cfDNAs from plasma of patients with lung cancer. (a) Comparison o f the concentration of <t>cfDNA</t> eluted from plasma of patients with lung cancer using NW networks and the <t>Qiagen</t> kit. (b) Detection of EGFR exon 21 L858R mutation in cfDNA isolated from plasma of patients with lung cancer, L6, using NW networks and the Qiagen kit. The mutation status of cfDNA was assessed using droplet digital PCR (ddPCR). (c) Assessment of the correlation between the number of NW networks and the concentration of cfDNA isolated, and cfDNA, as measured by ddPCR, in copies of EGFR mutation/µL. The photographs show in situ formation of NW networks in plasma of healthy control (H) and patients with lung cancer (L1, L2). When aggregates formed in plasma are transferred to water, they still do not dissolve. (D) Detection of EGFR exon 20 T790M mutation in cfDNA isolated from plasma of patients with lung cancer using NW networks (left) and evaluation of the mutation status after the introduction of enzymes, RNase (middle) or DNase (Right), in the cfDNA solution. The mutation status of cfDNA was assessed using droplet digital PCR (ddPCR).
    Qiagen Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/qiagen kit/product/Qiagen
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    qiagen kit - by Bioz Stars, 2021-03
    97/100 stars
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    Image Search Results


    NW networks as a highly efficient platform for isolation and analysis of cfDNAs from plasma of patients with lung cancer. (a) Comparison o f the concentration of cfDNA eluted from plasma of patients with lung cancer using NW networks and the Qiagen kit. (b) Detection of EGFR exon 21 L858R mutation in cfDNA isolated from plasma of patients with lung cancer, L6, using NW networks and the Qiagen kit. The mutation status of cfDNA was assessed using droplet digital PCR (ddPCR). (c) Assessment of the correlation between the number of NW networks and the concentration of cfDNA isolated, and cfDNA, as measured by ddPCR, in copies of EGFR mutation/µL. The photographs show in situ formation of NW networks in plasma of healthy control (H) and patients with lung cancer (L1, L2). When aggregates formed in plasma are transferred to water, they still do not dissolve. (D) Detection of EGFR exon 20 T790M mutation in cfDNA isolated from plasma of patients with lung cancer using NW networks (left) and evaluation of the mutation status after the introduction of enzymes, RNase (middle) or DNase (Right), in the cfDNA solution. The mutation status of cfDNA was assessed using droplet digital PCR (ddPCR).

    Journal: Theranostics

    Article Title: Magnetic Nanowire Networks for Dual-Isolation and Detection of Tumor-Associated Circulating Biomarkers

    doi: 10.7150/thno.21967

    Figure Lengend Snippet: NW networks as a highly efficient platform for isolation and analysis of cfDNAs from plasma of patients with lung cancer. (a) Comparison o f the concentration of cfDNA eluted from plasma of patients with lung cancer using NW networks and the Qiagen kit. (b) Detection of EGFR exon 21 L858R mutation in cfDNA isolated from plasma of patients with lung cancer, L6, using NW networks and the Qiagen kit. The mutation status of cfDNA was assessed using droplet digital PCR (ddPCR). (c) Assessment of the correlation between the number of NW networks and the concentration of cfDNA isolated, and cfDNA, as measured by ddPCR, in copies of EGFR mutation/µL. The photographs show in situ formation of NW networks in plasma of healthy control (H) and patients with lung cancer (L1, L2). When aggregates formed in plasma are transferred to water, they still do not dissolve. (D) Detection of EGFR exon 20 T790M mutation in cfDNA isolated from plasma of patients with lung cancer using NW networks (left) and evaluation of the mutation status after the introduction of enzymes, RNase (middle) or DNase (Right), in the cfDNA solution. The mutation status of cfDNA was assessed using droplet digital PCR (ddPCR).

    Article Snippet: A relatively low sensitivity for mutations was detected in the cfDNA isolated using the Qiagen kit.

    Techniques: Isolation, Concentration Assay, Mutagenesis, Digital PCR, In Situ

    Validation of PEI-mPpy NWs in the extraction of cfDNA in urine samples of cervical cancer patients. Comparisons of the concentration of cfDNA isolated from urine samples of ten representative cervical cancer patients by using PEI-mPpy NWs and a Qiagen DNA extraction kit. PEI-mPpy NWs and a Qiagen kit were employed for the extraction of cfDNA from urine.

    Journal: Theranostics

    Article Title: A Versatile Nanowire Platform for Highly Efficient Isolation and Direct PCR-free Colorimetric Detection of Human Papillomavirus DNA from Unprocessed Urine

    doi: 10.7150/thno.21696

    Figure Lengend Snippet: Validation of PEI-mPpy NWs in the extraction of cfDNA in urine samples of cervical cancer patients. Comparisons of the concentration of cfDNA isolated from urine samples of ten representative cervical cancer patients by using PEI-mPpy NWs and a Qiagen DNA extraction kit. PEI-mPpy NWs and a Qiagen kit were employed for the extraction of cfDNA from urine.

    Article Snippet: Of the 15 HPV-positive samples, HPV DNA was identified in all 15 (100%) when isolated by using PEI-mPpy NWs, whereas HPV DNA was detected in 5 urine samples (33.3%) when extracted with the Qiagen kit.

    Techniques: Concentration Assay, Isolation, DNA Extraction

    Evaluation of purification of tobacco genomic DNA and total RNA using filter paper-based spin columns with respective Qiagen kit buffers and homemade buffers. (A) Agarose gel electrophoresis for 2.5 μl tobacco genomic DNAs elution from purification experiments using Qiagen DNeasy plant mini kit buffers with Qiagen original spin column (Lane Q/Q), filter paper recharged used spin column (Lane Q/R) and filter paper-based homemade spin column (Lane Q/H*), followed by tobacco genomic DNAs purified using homemade buffer with Qiagen original spin column (Lane H/Q), filter paper recharged used spin column (Lane H/R) and filter paper-based homemade spin column (Lane H/H*). (B) UV spectrum curve of tobacco DNAs purified using Qiagen kit (Q/Q, black curve), filter paper recharged spin columns with Qiagen kit buffers (Q/R, blue curve) or homemade buffers (H/R, red curve) from the same amount leaf tissue. Y-axis is UV absorbance, and X-axis is wavelength (nM). (C) Amplification plots for three duplicated qPCR reactions contain 20 ng DNA purified using Qiagen kit (Q/Q, Blue curves) or DNA purified from filter paper recharged spin column with homemade buffer (H/R, Red curves) respectively. The x-axis is PCR cycle numbers, Y-axis is the level of SYBR fluorescence, and the green line is an arbitrary threshold to determine the Cq value (the fractional cycle number at which amplification curve meet threshold level). (D) MOPS-formaldehyde denaturing agarose gel electrophoresis separated 5 μl RNA purified using Qiagen RNeasy plant mini kit buffers with a Qiagen original spin column (Lane Q/Q), filter paper recharged used spin column (Lane Q/R) and homemade filter paper-based spin column (Lane Q/H*), followed total tobacco RNAs purified by using homemade buffer with Qiagen original spin column (Lane H/Q), filter paper recharged used spin column (Lane H/R) and filter paper-based homemade spin column (Lane H/H*). (E) UV spectrum of tobacco total RNA purified using Qiagen kit (Q/Q, black curve), filter paper recharged spin column with Qiagen RNeasy plant mini kit buffers (Q/R, blue curve) or homemade buffers (H/R, red curve). Y-axis is UV absorbance, and the X-axis is wavelength. (F) Amplification plots of three duplicated qRT-PCR reactions for 2.5 ng RNA purified using Qiagen kit (Q/Q, Blue curves) or RNA purified using filter paper recharged spin column with homemade buffer (H/R, Red curves) respectively. Note: * The starting material amount is 100 mg tobacco leaf tissue for experiments using a Qiagen spin column or filter paper recharged spin column, and half amount of plant sample (50 mg) used for homemade spin column purification. All DNAs or RNAs were eluted using 100 ul elution solution.

    Journal: PLoS ONE

    Article Title: Filter paper-based spin column method for cost-efficient DNA or RNA purification

    doi: 10.1371/journal.pone.0203011

    Figure Lengend Snippet: Evaluation of purification of tobacco genomic DNA and total RNA using filter paper-based spin columns with respective Qiagen kit buffers and homemade buffers. (A) Agarose gel electrophoresis for 2.5 μl tobacco genomic DNAs elution from purification experiments using Qiagen DNeasy plant mini kit buffers with Qiagen original spin column (Lane Q/Q), filter paper recharged used spin column (Lane Q/R) and filter paper-based homemade spin column (Lane Q/H*), followed by tobacco genomic DNAs purified using homemade buffer with Qiagen original spin column (Lane H/Q), filter paper recharged used spin column (Lane H/R) and filter paper-based homemade spin column (Lane H/H*). (B) UV spectrum curve of tobacco DNAs purified using Qiagen kit (Q/Q, black curve), filter paper recharged spin columns with Qiagen kit buffers (Q/R, blue curve) or homemade buffers (H/R, red curve) from the same amount leaf tissue. Y-axis is UV absorbance, and X-axis is wavelength (nM). (C) Amplification plots for three duplicated qPCR reactions contain 20 ng DNA purified using Qiagen kit (Q/Q, Blue curves) or DNA purified from filter paper recharged spin column with homemade buffer (H/R, Red curves) respectively. The x-axis is PCR cycle numbers, Y-axis is the level of SYBR fluorescence, and the green line is an arbitrary threshold to determine the Cq value (the fractional cycle number at which amplification curve meet threshold level). (D) MOPS-formaldehyde denaturing agarose gel electrophoresis separated 5 μl RNA purified using Qiagen RNeasy plant mini kit buffers with a Qiagen original spin column (Lane Q/Q), filter paper recharged used spin column (Lane Q/R) and homemade filter paper-based spin column (Lane Q/H*), followed total tobacco RNAs purified by using homemade buffer with Qiagen original spin column (Lane H/Q), filter paper recharged used spin column (Lane H/R) and filter paper-based homemade spin column (Lane H/H*). (E) UV spectrum of tobacco total RNA purified using Qiagen kit (Q/Q, black curve), filter paper recharged spin column with Qiagen RNeasy plant mini kit buffers (Q/R, blue curve) or homemade buffers (H/R, red curve). Y-axis is UV absorbance, and the X-axis is wavelength. (F) Amplification plots of three duplicated qRT-PCR reactions for 2.5 ng RNA purified using Qiagen kit (Q/Q, Blue curves) or RNA purified using filter paper recharged spin column with homemade buffer (H/R, Red curves) respectively. Note: * The starting material amount is 100 mg tobacco leaf tissue for experiments using a Qiagen spin column or filter paper recharged spin column, and half amount of plant sample (50 mg) used for homemade spin column purification. All DNAs or RNAs were eluted using 100 ul elution solution.

    Article Snippet: The qRT-PCR analysis indicated no significant difference in amplifications between RNA purified by the Qiagen kit and the RNA purified using a filter paper-based spin column with homemade buffers.

    Techniques: Purification, Agarose Gel Electrophoresis, Amplification, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Fluorescence, Quantitative RT-PCR

    Comparison of miRNAs expression profiles from human PBMCs and serum. Human PBMCs (1×10 6 cells) and serum (300 μL) samples were extracted by Macherey-Nagel (MN) and Qiagen (Q) kits. A - Number of miRNAs detected from PBMCs RNA samples using TLDA cards. B - Correlation analysis from PBMCs samples. C - Bland-Altman analysis MN versus Q from PBMCs. D - Number of miRNAs detected from serum RNA samples using TLDA cards. E - Correlation analysis from serum samples. F - Bland-Altman analysis MN versus Q from serum. TLDA data were obtained from biological triplicate for each extraction kits. Analysis using mean Ct values of triplicate. Only miRNAs with Ct

    Journal: BMC Genomics

    Article Title: Comparison of different extraction techniques to profile microRNAs from human sera and peripheral blood mononuclear cells

    doi: 10.1186/1471-2164-15-395

    Figure Lengend Snippet: Comparison of miRNAs expression profiles from human PBMCs and serum. Human PBMCs (1×10 6 cells) and serum (300 μL) samples were extracted by Macherey-Nagel (MN) and Qiagen (Q) kits. A - Number of miRNAs detected from PBMCs RNA samples using TLDA cards. B - Correlation analysis from PBMCs samples. C - Bland-Altman analysis MN versus Q from PBMCs. D - Number of miRNAs detected from serum RNA samples using TLDA cards. E - Correlation analysis from serum samples. F - Bland-Altman analysis MN versus Q from serum. TLDA data were obtained from biological triplicate for each extraction kits. Analysis using mean Ct values of triplicate. Only miRNAs with Ct

    Article Snippet: B: From serum (300 μL) samples, no cut-off. (PDF 1 MB) Additional file 3: Figure S3: Assessment of bias in RNA isolation from PBMCs samples using the Qiagen kit, comparison of extraction from 3×106 and 1×106 cells with the same amount of RNA for RT (110 ng).

    Techniques: Expressing, TLDA Assay