qiaamp circulating nucleic acid kit (Qiagen)
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QIAamp Circulating Nucleic Acid kit
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Catalog Number:
55114
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Structured Review
Qiagen
qiaamp circulating nucleic acid kit
https://www.bioz.com/result/qiaamp circulating nucleic acid kit/product/Qiagen
Average 99 stars, based on 212 article reviews
Price from $9.99 to $1999.99
https://www.bioz.com/result/qiaamp circulating nucleic acid kit/product/Qiagen
Average 99 stars, based on 212 article reviews
Price from $9.99 to $1999.99
qiaamp circulating nucleic acid kit - by Bioz Stars,
2019-12
99/100 stars
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1) Product Images from "Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pressure"
Article Title: Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pressure
Journal: Scientific Reports
doi: 10.1038/s41598-018-23766-9
Figure Legend Snippet: Comparison of extracted cfDNA concentration in qPCR assay. cfDNA concentration in a plasma sample was compared according to extraction methods, QIAamp and PIBEX. cfDNA amount in plasma sample was quantified by qPCR in four reference genes, TERT, RPPH1, GAPDH, and NAGK, in ( a ). The error bar represents standard deviation of triplex qPCR assay in a sample. The relative efficiency of cfDNA extraction in PIBEX was derived based on QIAamp in ( b ). The error bar is standard deviation of samples (n = 7).
Techniques Used: Concentration Assay, Real-time Polymerase Chain Reaction, Standard Deviation, Derivative Assay
2) Product Images from "Evaluation of pre-analytical conditions and comparison of the performance of several digital PCR assays for the detection of major EGFR mutations in circulating DNA from non-small cell lung cancers: the CIRCAN_0 study"
Article Title: Evaluation of pre-analytical conditions and comparison of the performance of several digital PCR assays for the detection of major EGFR mutations in circulating DNA from non-small cell lung cancers: the CIRCAN_0 study
Journal: Oncotarget
doi: 10.18632/oncotarget.21256
Figure Legend Snippet: Optimization of circulating free DNA (cfDNA) extraction and quantification of cfDNA in the samples (A) Reproducibility of cfDNA extraction using the QIAamp Circulating Acid Kit (Qiagen, Cat No 55114, Valencia, CA, USA) on two independent cfDNA samples extracted from 1 mL (Ai) and 3 mL (Aii) of plasma from NSCLC patients. After extraction, cfDNA was quantified by Qubit dsDNA HS Assay Kit (Life Technologies, Q32854, Carlsbad, CA, USA) according to the manufacturer's instructions. (B) Correlation between the initial volume of plasma 1 mL versus 3 mL (Bi) or 3 mL versus 5 mL (Bii) and the quantity of cfDNA extracted (in ng/μL). (Ci) Fragment size visualization of cfDNA (in bp) from a concentrated (left) and a less concentrated (right) sample obtained using the Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) (Cii) , and average size distribution (10 bp increments) of cfDNA fragments in 77 plasma samples. (D) Correlation between cfDNA concentration measured using the Qubit method and the number of amplifiable copies in the corresponding plasma samples determined using the Quantifiler Kit.
Techniques Used: Concentration Assay
Related Articles
Centrifugation:Article Title: Tracking plasma DNA mutation dynamics in estrogen receptor positive metastatic breast cancer with dPCR-SEQ Article Snippet: A QIAamp circulating nucleic acid kit (Qiagen, Valencia, CA, USA; Catalog No. 55114) was used to extract DNA from 2 to 5 ml of plasma according to the manufacturer’s protocol. .. A QIAamp circulating Article Title: Noninvasive Digital Detection of Fetal DNA in Plasma of 4-Week-Pregnant Women following In Vitro Fertilization and Embryo Transfer Article Snippet: Plasma CNA was extracted using QIAamp Circulating Article Title: Monitoring BRAF and NRAS mutations with cell-free circulating tumor DNA from metastatic melanoma patients Article Snippet: For the Idylla assay, DNA was extracted with the specific cartridge and on-board reagents, whereas for Ion Torrent PGM sequencing the DNA was extracted with the Kit QIAamp circulating Nucleic Acid (Qiagen, Hilden, Germany). .. For the Idylla assay, DNA was extracted with the specific cartridge and on-board reagents, whereas for Ion Torrent PGM sequencing the DNA was extracted with the Article Title: Walking pathways with positive feedback loops reveal DNA methylation biomarkers of colorectal cancer Article Snippet: After centrifugation, plasma samples were stored immediately at − 80 °C until cell-free DNA (cfDNA) extraction. .. Cell-free DNA was isolated using the Article Title: An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination Article Snippet: RNA was extracted using the QIAamp One-For-All Nucleic Acid Kit on an automated extraction platform (Qiagen BioRobot Universal System, Qiagen, UK) according to the manufacturer's instructions. .. RNA was extracted using the Amplification:Article Title: A comparison of two biological markers of recent hepatitis C virus (HCV) infection: implications for the monitoring of interventions and strategies to reduce HCV transmission among people who inject drugs Article Snippet: The entire eluate was extracted on the Qiagen Biorobot MDX platform using the Article Title: Site- and allele-specific polycomb dysregulation in T-cell leukaemia Article Snippet: Genomic DNA was extracted using Article Title: Characterization of Acute HIV-1 Infection in High-Risk Nigerian Populations Article Snippet: High-molecular-weight DNA was extracted from at least 2 × 105 PBMCs following the procedures of the Quantitative RT-PCR:Article Title: An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination Article Snippet: RNA was extracted using the Enzyme-linked Immunosorbent Assay:Article Title: TRAIL receptor I (DR4) polymorphisms C626G and A683C are associated with an increased risk for hepatocellular carcinoma (HCC) in HCV-infected patients Article Snippet: HCV antibodies were detected with a micro particle enzyme immunoassay (Axsym; Abbott) and confirmed by dot immunoassay (Matrix; Abbott). .. HCV RNA was detected with a Microarray:Article Title: Deciphering genetic regulation of CD14 by SP1 through characterization of peripheral blood mononuclear transcriptome of P. faiciparum and P. vivax infected malaria patients Article Snippet: Samples (n = 35) with total RNA concentration ≥ 25 ng/μl and RIN value 8 were selected for Illumina whole genome microarray and quantitative PCR analyses. .. Genomic DNA was extracted from peripheral blood leukocytes (n = 141) Incubation:Article Title: CAHM, a long non-coding RNA gene hypermethylated in colorectal neoplasia Article Snippet: Plasma DNA extraction and bisulphite conversion DNA was extracted from 4 mL plasma using the “QIAamp Circulating Article Title: Noninvasive Digital Detection of Fetal DNA in Plasma of 4-Week-Pregnant Women following In Vitro Fertilization and Embryo Transfer Article Snippet: Plasma CNA was extracted using QIAamp Circulating Article Title: An Integrated Next-Generation Sequencing System for Analyzing DNA Mutations, Gene Fusions, and RNA Expression in Lung Cancer Article Snippet: For each tissue specimen, multiple sections were scraped from the glass slides and combined into a single tube. .. The tissue was then incubated with xylene to remove residual paraffin followed by extraction using Article Title: Functional diversity and co-operativity between subclonal populations of paediatric glioblastoma and diffuse intrinsic pontine glioma cells Article Snippet: Slides were hydrated through an ethanol series prior to manual microdissection into a tube using a sterile fine needle. .. All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the Formalin-fixed Paraffin-Embedded:Article Title: Non-invasive diagnosis of early-stage lung cancer using high-throughput targeted DNA methylation sequencing of circulating tumor DNA (ctDNA) Article Snippet: Tissue genomic DNA (gDNA) was isolated from FFPE tissue samples using the Qiagen QIAamp DNA FFPE Tissue Kit (Qiagen, Cat# 56404) according to the manufacture's protocol. gDNA was fragmented to 200 bp using the M220 Focused-ultrasonicator™ (Covaris, Inc.) following the manufacturer's protocol and 100 ng of fragmented DNA was used for library construction. .. For plasma collected using Streck BCT, cfDNA was isolated using the Qiagen QIAamp Circulating Article Title: An Integrated Next-Generation Sequencing System for Analyzing DNA Mutations, Gene Fusions, and RNA Expression in Lung Cancer Article Snippet: For each tissue specimen, multiple sections were scraped from the glass slides and combined into a single tube. .. The tissue was then incubated with xylene to remove residual paraffin followed by extraction using Article Title: Functional diversity and co-operativity between subclonal populations of paediatric glioblastoma and diffuse intrinsic pontine glioma cells Article Snippet: Slides were hydrated through an ethanol series prior to manual microdissection into a tube using a sterile fine needle. .. All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the Modification:Article Title: CAHM, a long non-coding RNA gene hypermethylated in colorectal neoplasia Article Snippet: Plasma DNA extraction and bisulphite conversion DNA was extracted from 4 mL plasma using the “QIAamp Circulating Article Title: Altered LINE-1 Methylation in Mothers of Children with Down Syndrome Article Snippet: Genomic DNA was isolated from frozen blood samples using the Article Title: Maternal LINE-1 DNA Methylation and Congenital Heart Defects in Down Syndrome Article Snippet: Genomic DNA was extracted from peripheral blood leukocytes using the Ligation:Article Title: Site- and allele-specific polycomb dysregulation in T-cell leukaemia Article Snippet: Paragraph title: Ligation-mediated PCR ... Genomic DNA was extracted using Infection:Article Title: TRAIL receptor I (DR4) polymorphisms C626G and A683C are associated with an increased risk for hepatocellular carcinoma (HCC) in HCV-infected patients Article Snippet: Paragraph title: Diagnosis of HCV Infection ... HCV RNA was detected with a Article Title: A comparison of two biological markers of recent hepatitis C virus (HCV) infection: implications for the monitoring of interventions and strategies to reduce HCV transmission among people who inject drugs Article Snippet: The entire eluate was extracted on the Qiagen Biorobot MDX platform using the other:Article Title: Increased Plasma Circulating Cell-Free DNA Could Be a Potential Marker for Oral Cancer Article Snippet: The separation and storage of the plasma samples was performed within 3 h of blood collection at 4 °C. cfDNA was extracted from 1 mL plasma aliquots using the QIAamp Circulating Sequencing:Article Title: Monitoring BRAF and NRAS mutations with cell-free circulating tumor DNA from metastatic melanoma patients Article Snippet: The plasma was obtained by two rounds of centrifugation (10 minutes, 2000 g at 4°C) aliquoted into 1mL tubes and stored at −80°C until use. .. For the Idylla assay, DNA was extracted with the specific cartridge and on-board reagents, whereas for Ion Torrent PGM sequencing the DNA was extracted with the Article Title: Walking pathways with positive feedback loops reveal DNA methylation biomarkers of colorectal cancer Article Snippet: Cell-free DNA was isolated using the Article Title: Characterization of Acute HIV-1 Infection in High-Risk Nigerian Populations Article Snippet: Paragraph title: HIV-1 Sequencing ... High-molecular-weight DNA was extracted from at least 2 × 105 PBMCs following the procedures of the Article Title: Functional diversity and co-operativity between subclonal populations of paediatric glioblastoma and diffuse intrinsic pontine glioma cells Article Snippet: All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the Nucleic Acid Purification:Article Title: TRAIL receptor I (DR4) polymorphisms C626G and A683C are associated with an increased risk for hepatocellular carcinoma (HCC) in HCV-infected patients Article Snippet: HCV antibodies were detected with a micro particle enzyme immunoassay (Axsym; Abbott) and confirmed by dot immunoassay (Matrix; Abbott). .. HCV RNA was detected with a DNA Extraction:Article Title: CAHM, a long non-coding RNA gene hypermethylated in colorectal neoplasia Article Snippet: Enzymatically methylated human DNA (CpGenome Universal Methylated DNA, Millipore, Cat. S7821) was used as a positive methylation control, and white blood cell DNA (WBC DNA, Human Genomic DNA from human buffy coat, Roche Applied Science, Cat. 1169111201) as an unmethylated control. .. Plasma DNA extraction and bisulphite conversion DNA was extracted from 4 mL plasma using the “QIAamp Circulating Article Title: Noninvasive Digital Detection of Fetal DNA in Plasma of 4-Week-Pregnant Women following In Vitro Fertilization and Embryo Transfer Article Snippet: Paragraph title: Plasma DNA Extraction ... Plasma CNA was extracted using QIAamp Circulating Article Title: Analysis of solid tumor mutation profiles in liquid biopsy, et al. Analysis of solid tumor mutation profiles in liquid biopsy Article Snippet: Paragraph title: Cell‐free DNA extraction from blood ... Total cfDNA was extracted from plasma using QIAamp Circulating Article Title: Characterization of Acute HIV-1 Infection in High-Risk Nigerian Populations Article Snippet: Peripheral blood mononuclear cells (PBMCs) were purified from the rest of blood components by the Ficoll Hypaque procedure, and the PBMCs were used for DNA extraction. .. High-molecular-weight DNA was extracted from at least 2 × 105 PBMCs following the procedures of the Methylation:Article Title: CAHM, a long non-coding RNA gene hypermethylated in colorectal neoplasia Article Snippet: Plasma DNA extraction and bisulphite conversion DNA was extracted from 4 mL plasma using the “QIAamp Circulating Article Title: Walking pathways with positive feedback loops reveal DNA methylation biomarkers of colorectal cancer Article Snippet: Cell-free DNA was isolated using the Isolation:Article Title: Analysis of solid tumor mutation profiles in liquid biopsy, et al. Analysis of solid tumor mutation profiles in liquid biopsy Article Snippet: Total cfDNA was extracted from plasma using QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat. No: 55114), as per standard protocol. .. Total cfDNA was extracted from plasma using QIAamp Circulating Article Title: Non-invasive diagnosis of early-stage lung cancer using high-throughput targeted DNA methylation sequencing of circulating tumor DNA (ctDNA) Article Snippet: Tissue genomic DNA (gDNA) was isolated from FFPE tissue samples using the Qiagen QIAamp DNA FFPE Tissue Kit (Qiagen, Cat# 56404) according to the manufacture's protocol. gDNA was fragmented to 200 bp using the M220 Focused-ultrasonicator™ (Covaris, Inc.) following the manufacturer's protocol and 100 ng of fragmented DNA was used for library construction. .. For plasma collected using Streck BCT, cfDNA was isolated using the Qiagen QIAamp Circulating Article Title: Walking pathways with positive feedback loops reveal DNA methylation biomarkers of colorectal cancer Article Snippet: After centrifugation, plasma samples were stored immediately at − 80 °C until cell-free DNA (cfDNA) extraction. .. Cell-free DNA was isolated using the Article Title: An Integrated Next-Generation Sequencing System for Analyzing DNA Mutations, Gene Fusions, and RNA Expression in Lung Cancer Article Snippet: The tissue was then incubated with xylene to remove residual paraffin followed by extraction using Article Title: Deciphering genetic regulation of CD14 by SP1 through characterization of peripheral blood mononuclear transcriptome of P. faiciparum and P. vivax infected malaria patients Article Snippet: Total RNA was isolated following manufacturer's protocol using RNeasy® Plus Minikit (QIAGEN) and quantified using nanodrop 2000C spectrophotometer (Thermo Scientific). .. Genomic DNA was extracted from peripheral blood leukocytes (n = 141) Article Title: Altered LINE-1 Methylation in Mothers of Children with Down Syndrome Article Snippet: The questionnaire was adapted from a food frequency questionnaire validated for the Croatian women [ ]. .. Genomic DNA was isolated from frozen blood samples using the Purification:Article Title: The molecular evolution of acquired resistance to targeted EGFR blockade in colorectal cancers Article Snippet: KRAS mutational status in the tissue was predetermined using the DxS assay (Qiagen). .. DNA was purified from of 0.2–1mL of banked serum using the QIAamp circulating Article Title: Genome-Wide Plasma Cell-Free DNA Methylation Profiling Identifies Potential Biomarkers for Lung Cancer Article Snippet: All blood samples from the control group and lung cancer patients (∼5 ml) were collected in tubes containing EDTA as anticoagulant, centrifuged for 15 min at 1500 × g. The purified plasma was then stored at -80°C. .. The cfDNA was extracted from plasma using QIAamp Circulating Article Title: Site- and allele-specific polycomb dysregulation in T-cell leukaemia Article Snippet: Genomic DNA was extracted using Article Title: Characterization of Acute HIV-1 Infection in High-Risk Nigerian Populations Article Snippet: Peripheral blood mononuclear cells (PBMCs) were purified from the rest of blood components by the Ficoll Hypaque procedure, and the PBMCs were used for DNA extraction. .. High-molecular-weight DNA was extracted from at least 2 × 105 PBMCs following the procedures of the Polymerase Chain Reaction:Article Title: Walking pathways with positive feedback loops reveal DNA methylation biomarkers of colorectal cancer Article Snippet: Cell-free DNA was isolated using the Article Title: An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination Article Snippet: RNA was extracted using the Article Title: A comparison of two biological markers of recent hepatitis C virus (HCV) infection: implications for the monitoring of interventions and strategies to reduce HCV transmission among people who inject drugs Article Snippet: The entire eluate was extracted on the Qiagen Biorobot MDX platform using the Article Title: Site- and allele-specific polycomb dysregulation in T-cell leukaemia Article Snippet: Paragraph title: Ligation-mediated PCR ... Genomic DNA was extracted using Article Title: Characterization of Acute HIV-1 Infection in High-Risk Nigerian Populations Article Snippet: High-molecular-weight DNA was extracted from at least 2 × 105 PBMCs following the procedures of the Article Title: Altered LINE-1 Methylation in Mothers of Children with Down Syndrome Article Snippet: Genomic DNA was isolated from frozen blood samples using the Article Title: Maternal LINE-1 DNA Methylation and Congenital Heart Defects in Down Syndrome Article Snippet: Genomic DNA was extracted from peripheral blood leukocytes using the Nested PCR:Article Title: TRAIL receptor I (DR4) polymorphisms C626G and A683C are associated with an increased risk for hepatocellular carcinoma (HCC) in HCV-infected patients Article Snippet: HCV antibodies were detected with a micro particle enzyme immunoassay (Axsym; Abbott) and confirmed by dot immunoassay (Matrix; Abbott). .. HCV RNA was detected with a Sample Prep:Article Title: Walking pathways with positive feedback loops reveal DNA methylation biomarkers of colorectal cancer Article Snippet: Paragraph title: Sample preparation protocol ... Cell-free DNA was isolated using the Real-time Polymerase Chain Reaction:Article Title: An infected chicken kidney cell co-culture ELISpot for enhanced detection of T cell responses to avian influenza and vaccination Article Snippet: RNA was extracted using the Article Title: Deciphering genetic regulation of CD14 by SP1 through characterization of peripheral blood mononuclear transcriptome of P. faiciparum and P. vivax infected malaria patients Article Snippet: Samples (n = 35) with total RNA concentration ≥ 25 ng/μl and RIN value 8 were selected for Illumina whole genome microarray and quantitative PCR analyses. .. Genomic DNA was extracted from peripheral blood leukocytes (n = 141) Agarose Gel Electrophoresis:Article Title: Site- and allele-specific polycomb dysregulation in T-cell leukaemia Article Snippet: Genomic DNA was extracted using Homogenization:Article Title: Functional diversity and co-operativity between subclonal populations of paediatric glioblastoma and diffuse intrinsic pontine glioma cells Article Snippet: DNA was extracted from frozen tissue by homogenisation prior to following the DNeasy Blood & Tissue kit protocol (Qiagen, Crawley, UK). .. All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the Laser Capture Microdissection:Article Title: Functional diversity and co-operativity between subclonal populations of paediatric glioblastoma and diffuse intrinsic pontine glioma cells Article Snippet: All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the QIAamp DNA FFPE tissue kit protocol (Qiagen, Crawley, UK) using 360μl of Buffer AL, 360μl of ethanol and eluted using 25μl of 10mM Tris buffer at pH8.5 for 7 minutes. .. All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the Spectrophotometry:Article Title: Deciphering genetic regulation of CD14 by SP1 through characterization of peripheral blood mononuclear transcriptome of P. faiciparum and P. vivax infected malaria patients Article Snippet: Total RNA was isolated following manufacturer's protocol using RNeasy® Plus Minikit (QIAGEN) and quantified using nanodrop 2000C spectrophotometer (Thermo Scientific). .. Genomic DNA was extracted from peripheral blood leukocytes (n = 141) Article Title: Altered LINE-1 Methylation in Mothers of Children with Down Syndrome Article Snippet: Genomic DNA was isolated from frozen blood samples using the Sampling:Article Title: Functional diversity and co-operativity between subclonal populations of paediatric glioblastoma and diffuse intrinsic pontine glioma cells Article Snippet: We obtained longitudinal paired samples from two patients from the Centre Hospitalier Régional et Universitaire Hautepierre, Strasbourg, France; five DIPG patients with multiple sampling taken at autopsy from the Hospital San Joan de Deu, Barcelona, Spain; five DIPG patients with multiple sampling taken at autopsy from Stanford Medical School, Stanford, CA, USA; three cases with multiple samples from the Queensland Children’s Tumour Bank, Brisbane, Australia; one case each with multiple samples from St Georges Hospital and Kings College Hospital, London, UK , all of which were collected locally after informed consent. .. All tissue was incubated overnight with proteinase K at 56°C with a further incubation for 3 hours the following morning, prior to following the Concentration Assay:Article Title: Non-invasive diagnosis of early-stage lung cancer using high-throughput targeted DNA methylation sequencing of circulating tumor DNA (ctDNA) Article Snippet: For plasma collected using Streck BCT, cfDNA was isolated using the Qiagen QIAamp Circulating Article Title: Deciphering genetic regulation of CD14 by SP1 through characterization of peripheral blood mononuclear transcriptome of P. faiciparum and P. vivax infected malaria patients Article Snippet: Samples (n = 35) with total RNA concentration ≥ 25 ng/μl and RIN value 8 were selected for Illumina whole genome microarray and quantitative PCR analyses. .. Genomic DNA was extracted from peripheral blood leukocytes (n = 141) DNA Purification:Article Title: The molecular evolution of acquired resistance to targeted EGFR blockade in colorectal cancers Article Snippet: Paragraph title: DNA purification ... DNA was purified from of 0.2–1mL of banked serum using the QIAamp circulating Lysis:Article Title: A comparison of two biological markers of recent hepatitis C virus (HCV) infection: implications for the monitoring of interventions and strategies to reduce HCV transmission among people who inject drugs Article Snippet: RNA testing involved elution from the DBS by incubating the 6 mm spot for 2 hours at 56 °C with 20 µL of proteinase K and 300 µL of ATL lysis buffer (Qiagen products: 19133 and 19076). .. The entire eluate was extracted on the Qiagen Biorobot MDX platform using the |