Journal: bioRxiv

Article Title: Involvement of ILC1-like innate lymphocytes in human autoimmunity, lessons from alopecia areata

doi: 10.1101/2022.06.22.497177

Figure Lengend Snippet: ( A ) ILC1-like cells (EOMES+, CD49a+ and NKG2D+) around HF in normal scalp skin, intrafollicular and perifollicular ILC1-like cells infiltrates in lesional and in non-lesional AA scalp patient.( B ) EOMES+,c-KIT−,CD49a+ and ( C ) NKp44+, CD103+, T-bet-ILC1-like cells. ( D ) Quantitative immunohistomorphometry (qIHM) shows increased number of ILC1-like cells in AA patients as compared to normal volunteers and increased number of the cells in lesional versus non-lesional areas of the patients. There is a significant increased perifollicular than intrafollicular ILC1-like cells in the lesional and non lesional areas. ( E ) CD8+/NKG2D+ cells around HF in AA scalp patient and absence of these cells in normal scalp skin of normal scalp skin. ( F ) There is an increased number of CD8+/NKG2D+ cells in HFs of AA patients compared to normal scalp skin and a significant lower number of ILC1-like cells versus CD8+/NKG2D+ cells in AA scalp skin. N=6 biopsies /AA patients and 6 biopsies /healthy donors from 6 independent donors, 3 areas were evaluated per section, and 3 sections per biopsy. Following Shapiro-Wilk test, Student’s t -test: *p < 0.05, ** p < 0.01 or Mann Whitney U test: # p < 0.05. Scale bars, 50 µm. CTS - connective tissue sheath, DP - dermal papilla, HM - hair matrix, White arrow-c-KIT stained melanocyte.

Article Snippet: Following gating on lineage, cell population cells were sorted as follows: ILC1-like cells – APC-conjugated-CD127+ (BLG-351315), PE/CY7-conjugated-CD161+ (BLG-339917), and FITC-conjugated-NKp44 + (SC-53597), Brilliant Violet 421TM anti-human CD117- (c-KIT) (BLG-313215) and APC/CY7-conjugated-CRTH2- (BLG-350113). (Santa Cruz H3112) ( ; ).

Techniques: MANN-WHITNEY, Staining

Journal: bioRxiv

Article Title: Involvement of ILC1-like innate lymphocytes in human autoimmunity, lessons from alopecia areata

doi: 10.1101/2022.06.22.497177

Figure Lengend Snippet: (A) EOMES+, CD49a+ and NKG2D+ around HF in normal scalp skin, intrafollicular and perifollicular ILC1-like cells infiltrates in AA-induced xenotransplants ( B ) EOMES+,c-KIT−,CD49a+ and ( C ) NKp44+, CD103+, T-bet-ILC1-like cells. Absence of these cells in normal scalp xenotransplant. ( D ) Quantitation. ( E ) CD8+/NKG2D+ cells around HF in AA-induced xenotransplant versus absence of the cells in normal xenotransplant. ( F ) The quantitative data demonstrate the significant increased CD8+/NKG2D+ cells in HFs of AA humanized mice compared to normal scalp xenotransplants. N=6 xenotransplants/ group from 3 independent donors, 3 areas were evaluated per section. Following Shapiro-Wilk test, Student’s t -test: * p < 0.05, ** p < 0.01, *** p < 0.001. Mann Whitney U test: # p < 0.05, ## p < 0.01. Scale bar, 50 µm. DP - dermal papilla, HM - hair matrix, White arrow-c-KIT stained melanocyte.

Article Snippet: Following gating on lineage, cell population cells were sorted as follows: ILC1-like cells – APC-conjugated-CD127+ (BLG-351315), PE/CY7-conjugated-CD161+ (BLG-339917), and FITC-conjugated-NKp44 + (SC-53597), Brilliant Violet 421TM anti-human CD117- (c-KIT) (BLG-313215) and APC/CY7-conjugated-CRTH2- (BLG-350113). (Santa Cruz H3112) ( ; ).

Techniques: Quantitation Assay, MANN-WHITNEY, Staining