rna (Qiagen)


Name:
QIAamp Viral RNA Mini Kit
Description:
For isolation of viral RNA from cell free body fluids Kit contents Qiagen QIAamp Viral RNA Mini Kit 50 preps 140L Sample 50L Elution Volume Liquid Media Sample Viral RNA Purification Silica Technology Spin Column Format Manual Processing 90 Yield 20 to 40 min Time Run Ideal for PCR qPCR Real time PCR For Isolation of Viral RNA from Cell free Body Fluids Includes 50 QIAamp Mini Spin Columns Carrier RNA 2mL Collection Tubes RNase free Buffers Benefits Rapid isolation of high quality ready to use RNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors
Catalog Number:
52904
Price:
257
Category:
QIAamp Viral RNA Mini Kit
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Structured Review
For isolation of viral RNA from cell free body fluids Kit contents Qiagen QIAamp Viral RNA Mini Kit 50 preps 140L Sample 50L Elution Volume Liquid Media Sample Viral RNA Purification Silica Technology Spin Column Format Manual Processing 90 Yield 20 to 40 min Time Run Ideal for PCR qPCR Real time PCR For Isolation of Viral RNA from Cell free Body Fluids Includes 50 QIAamp Mini Spin Columns Carrier RNA 2mL Collection Tubes RNase free Buffers Benefits Rapid isolation of high quality ready to use RNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors
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Price from $9.99 to $1999.99
Images
1) Product Images from "NEDD4 family ubiquitin ligases associate with LCMV Z’s PPXY domain and are required for virus budding, but not via direct ubiquitination of Z"
Article Title: NEDD4 family ubiquitin ligases associate with LCMV Z’s PPXY domain and are required for virus budding, but not via direct ubiquitination of Z
Journal: PLoS Pathogens
doi: 10.1371/journal.ppat.1008100

Figure Legend Snippet: Loss of ubiquitination sites in LCMV Z has little impact on virus particle release. (A-B) Reverse genetics was used to generate recombinant (r)LCMV containing mutations at the two ubiquitination sites (K10 and K77) as well as a virus with a lysine-free (NoK) or a late domain-free (PPPY > AAAA) Z protein. The growth kinetics of these viruses were then measured by infecting A549 cells with these viruses and measuring the infectious titers by plaque assay (A) and DI particle titers by plaque interfering assay (B) at 1, 16, 24, 40, 48 and 72 hours after infection. (C) Summary of a two-way ANOVA with Holm-Sidak’s test for multiple comparisons used to compare the log-transformed mean titer values from the growth curve in (A-B). (D-E) Viral RNA was isolated from clarified cell culture media from the 72 hours post-infection time point in (A-B) and the quantities of genomic S-segment (D) or L-segment (E) vRNA were determined by quantitative real time RT-PCR. (F) The release of virus-like particles (VLPs) from HEK293T cells transfected with plasmids expressing SBP-tagged LCMV Z protein with the indicated mutations was measured. The graphs in (A-B and D-F) represent the mean values ± SEM of three independent experiments with two technical replicates each. A one-way ANOVA with Holm-Sidak’s test for multiple comparisons was used to compare the mean values to the WT control in (D-F). *p
Techniques Used: Recombinant, Plaque Assay, Infection, Transformation Assay, Isolation, Cell Culture, Quantitative RT-PCR, Transfection, Expressing
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