rna  (Qiagen)


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    Name:
    QIAamp Viral RNA Mini Kit
    Description:
    For isolation of viral RNA from cell free body fluids Kit contents Qiagen QIAamp Viral RNA Mini Kit 50 preps 140L Sample 50L Elution Volume Liquid Media Sample Viral RNA Purification Silica Technology Spin Column Format Manual Processing 90 Yield 20 to 40 min Time Run Ideal for PCR qPCR Real time PCR For Isolation of Viral RNA from Cell free Body Fluids Includes 50 QIAamp Mini Spin Columns Carrier RNA 2mL Collection Tubes RNase free Buffers Benefits Rapid isolation of high quality ready to use RNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors
    Catalog Number:
    52904
    Price:
    257
    Category:
    QIAamp Viral RNA Mini Kit
    		Buy from Supplier

    Structured Review

    Qiagen rna
    QIAamp Viral RNA Mini Kit
    For isolation of viral RNA from cell free body fluids Kit contents Qiagen QIAamp Viral RNA Mini Kit 50 preps 140L Sample 50L Elution Volume Liquid Media Sample Viral RNA Purification Silica Technology Spin Column Format Manual Processing 90 Yield 20 to 40 min Time Run Ideal for PCR qPCR Real time PCR For Isolation of Viral RNA from Cell free Body Fluids Includes 50 QIAamp Mini Spin Columns Carrier RNA 2mL Collection Tubes RNase free Buffers Benefits Rapid isolation of high quality ready to use RNA No organic extraction or alcohol precipitation Consistent high yields Complete removal of contaminants and inhibitors
    https://www.bioz.com/result/rna/product/Qiagen
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rna - by Bioz Stars, 2021-03
    97/100 stars

    Images

    1) Product Images from "NEDD4 family ubiquitin ligases associate with LCMV Z’s PPXY domain and are required for virus budding, but not via direct ubiquitination of Z"

    Article Title: NEDD4 family ubiquitin ligases associate with LCMV Z’s PPXY domain and are required for virus budding, but not via direct ubiquitination of Z

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1008100

    Loss of ubiquitination sites in LCMV Z has little impact on virus particle release. (A-B) Reverse genetics was used to generate recombinant (r)LCMV containing mutations at the two ubiquitination sites (K10 and K77) as well as a virus with a lysine-free (NoK) or a late domain-free (PPPY > AAAA) Z protein. The growth kinetics of these viruses were then measured by infecting A549 cells with these viruses and measuring the infectious titers by plaque assay (A) and DI particle titers by plaque interfering assay (B) at 1, 16, 24, 40, 48 and 72 hours after infection. (C) Summary of a two-way ANOVA with Holm-Sidak’s test for multiple comparisons used to compare the log-transformed mean titer values from the growth curve in (A-B). (D-E) Viral RNA was isolated from clarified cell culture media from the 72 hours post-infection time point in (A-B) and the quantities of genomic S-segment (D) or L-segment (E) vRNA were determined by quantitative real time RT-PCR. (F) The release of virus-like particles (VLPs) from HEK293T cells transfected with plasmids expressing SBP-tagged LCMV Z protein with the indicated mutations was measured. The graphs in (A-B and D-F) represent the mean values ± SEM of three independent experiments with two technical replicates each. A one-way ANOVA with Holm-Sidak’s test for multiple comparisons was used to compare the mean values to the WT control in (D-F). *p
    Figure Legend Snippet: Loss of ubiquitination sites in LCMV Z has little impact on virus particle release. (A-B) Reverse genetics was used to generate recombinant (r)LCMV containing mutations at the two ubiquitination sites (K10 and K77) as well as a virus with a lysine-free (NoK) or a late domain-free (PPPY > AAAA) Z protein. The growth kinetics of these viruses were then measured by infecting A549 cells with these viruses and measuring the infectious titers by plaque assay (A) and DI particle titers by plaque interfering assay (B) at 1, 16, 24, 40, 48 and 72 hours after infection. (C) Summary of a two-way ANOVA with Holm-Sidak’s test for multiple comparisons used to compare the log-transformed mean titer values from the growth curve in (A-B). (D-E) Viral RNA was isolated from clarified cell culture media from the 72 hours post-infection time point in (A-B) and the quantities of genomic S-segment (D) or L-segment (E) vRNA were determined by quantitative real time RT-PCR. (F) The release of virus-like particles (VLPs) from HEK293T cells transfected with plasmids expressing SBP-tagged LCMV Z protein with the indicated mutations was measured. The graphs in (A-B and D-F) represent the mean values ± SEM of three independent experiments with two technical replicates each. A one-way ANOVA with Holm-Sidak’s test for multiple comparisons was used to compare the mean values to the WT control in (D-F). *p

    Techniques Used: Recombinant, Plaque Assay, Infection, Transformation Assay, Isolation, Cell Culture, Quantitative RT-PCR, Transfection, Expressing

    Related Articles

    Quantitative RT-PCR:

    Article Title: Antiviral Hammerhead Ribozymes Are Effective for Developing Transgenic Suppression of Chikungunya Virus in Aedes aegypti Mosquitoes
    Article Snippet: Caspase 3 activity was quantitated by measuring luminescence using a LMAX-2 luminometer (Molecular Devices, Sunnyvale, CA, USA). .. Quantitative RT-PCR Viral RNA was extracted at 3 dpi from the hRz clonals exhibiting complete suppression by TCID50 -IFA using a Mini Viral RNA kit (Qiagen, Valencia, CA, USA). ..

    Immunofluorescence:

    Article Title: Antiviral Hammerhead Ribozymes Are Effective for Developing Transgenic Suppression of Chikungunya Virus in Aedes aegypti Mosquitoes
    Article Snippet: Caspase 3 activity was quantitated by measuring luminescence using a LMAX-2 luminometer (Molecular Devices, Sunnyvale, CA, USA). .. Quantitative RT-PCR Viral RNA was extracted at 3 dpi from the hRz clonals exhibiting complete suppression by TCID50 -IFA using a Mini Viral RNA kit (Qiagen, Valencia, CA, USA). ..

    other:

    Article Title: Nanotrap® particles improve detection of SARS-CoV-2 for pooled sample methods, extraction-free saliva methods, and extraction-free transport medium methods
    Article Snippet: For the QIAamp Viral RNA Mini Kit, nucleic acids were extracted from 150 µL of sample according to the manufacturers’ protocol.

    Article Title: Efficient Extraction of Viral DNA and Viral RNA by the Chemagic Viral DNA/RNA Kit Allows Sensitive Detection of Cytomegalovirus, Hepatitis B Virus, and Hepatitis G Virus by PCR
    Article Snippet: Identical results were obtained after extraction of the identical specimens with the QIAamp Viral RNA Mini kit.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Differential Detection of Encapsidated versus Unencapsidated Enterovirus RNA in Samples Containing Pancreatic Enzymes—Relevance for Diabetes Studies
    Article Snippet: Blinded samples were tested at the three nPOD-V laboratories that used five different RT-PCR methods for EV detection. .. Virus Detection by RT-PCR Tampere laboratory: RNA was extracted from pancreas homogenate samples using the Viral RNA Kit (Qiagen, Hilden, Germany). .. Samples were analyzed using two RT-PCR methods: a semi-quantitative RT-PCR coupled with hybridization with an EV-specific probe (PCR 1) [ ], and a quantitative real-time RT-PCR (PCR 2) [ ].

    Article Title: DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP
    Article Snippet: RNA Extraction .. University of Vermont To compare the effect of nucleic acid extraction to direct RT-PCR, RNA from a pooled patient sample was extracted using the QIAamp Viral RNA Mini (Qiagen, Cat. No. 52904) kit according to the manufacturer’s instructions. .. 140 µL of pooled nasopharyngeal swab diluent was extracted and eluted in 60 µL; 5 µL (equivalent to 11 µL of original sample) was used for real-time RT-PCR .

    Isolation:

    Article Title: Structural basis for human respiratory syncytial virus NS1-mediated modulation of host responses
    Article Snippet: Medium was harvested at 2, 48, 72, and 96 hours post infection and clarified by centrifugation. .. RNA was isolated from medium using Qiagen Viral RNA Mini Kit and hRSV titer was determined by qPCR and expressed as fold change compared to 2 hour time point. .. Quantitative qPCR was conducted by using TaqMan Fast Virus 1-Step Master Mixture (Applied Biosystem) according to the manufacturer’s instructions.

    Real-time Polymerase Chain Reaction:

    Article Title: Structural basis for human respiratory syncytial virus NS1-mediated modulation of host responses
    Article Snippet: Medium was harvested at 2, 48, 72, and 96 hours post infection and clarified by centrifugation. .. RNA was isolated from medium using Qiagen Viral RNA Mini Kit and hRSV titer was determined by qPCR and expressed as fold change compared to 2 hour time point. .. Quantitative qPCR was conducted by using TaqMan Fast Virus 1-Step Master Mixture (Applied Biosystem) according to the manufacturer’s instructions.

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    • mini viral rna kit  (Qiagen)
    97
    Qiagen mini viral rna kit
    Generalized structure of a minimal hammerhead ribozyme <t>(hRz)</t> and organization of the chikungunya virus (CHIKV) viral genome showing hRz target sites. ( A ) hRzs consist of helix I and III which mediate complementary base-pairing with the target, and helix II which acts as the catalytic core mediating cleavage of the target <t>RNA;</t> ( B ) Schematic of the CHIKV genome. nsP1–nsP4 = polyprotein encoded by the genomic RNA. Capsid, E3, E2, 6K and E1 = polyprotein encoded by the sub-genomic RNA. 9–15 = target sites for hRz#9-#15.
    Mini Viral Rna Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mini viral rna kit/product/Qiagen
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mini viral rna kit - by Bioz Stars, 2021-03
    97/100 stars
    		  Buy from Supplier

    Image Search Results


    Generalized structure of a minimal hammerhead ribozyme (hRz) and organization of the chikungunya virus (CHIKV) viral genome showing hRz target sites. ( A ) hRzs consist of helix I and III which mediate complementary base-pairing with the target, and helix II which acts as the catalytic core mediating cleavage of the target RNA; ( B ) Schematic of the CHIKV genome. nsP1–nsP4 = polyprotein encoded by the genomic RNA. Capsid, E3, E2, 6K and E1 = polyprotein encoded by the sub-genomic RNA. 9–15 = target sites for hRz#9-#15.

    Journal: Viruses

    Article Title: Antiviral Hammerhead Ribozymes Are Effective for Developing Transgenic Suppression of Chikungunya Virus in Aedes aegypti Mosquitoes

    doi: 10.3390/v8060163

    Figure Lengend Snippet: Generalized structure of a minimal hammerhead ribozyme (hRz) and organization of the chikungunya virus (CHIKV) viral genome showing hRz target sites. ( A ) hRzs consist of helix I and III which mediate complementary base-pairing with the target, and helix II which acts as the catalytic core mediating cleavage of the target RNA; ( B ) Schematic of the CHIKV genome. nsP1–nsP4 = polyprotein encoded by the genomic RNA. Capsid, E3, E2, 6K and E1 = polyprotein encoded by the sub-genomic RNA. 9–15 = target sites for hRz#9-#15.

    Article Snippet: Quantitative RT-PCR Viral RNA was extracted at 3 dpi from the hRz clonals exhibiting complete suppression by TCID50 -IFA using a Mini Viral RNA kit (Qiagen, Valencia, CA, USA).

    Techniques:

    Amplification of a serial dilution of the EUROHEP standard with HBV-specific primers in a nested PCR following extraction with the Chemagic DNA/RNA kit and the QIAamp DNA Blood Mini kit. (A) Chemagen extracts. (B) QIAGEN extracts. Lane M shows a 100-bp ladder, and lane C shows results for the negative control. Lanes 1 through 10 show results for different viral loads expressed in numbers of copies per ml: 1, 4 × 10 5 ; 2, 4 × 10 4 ; 3, 4 × 10 3 ; 4, 4 × 10 2 ; 5, 2 × 10 2 ; 6, 1 × 10 2 ; 7, 8 × 10 1 ; 8, 4 × 10 1 ; 9, 2 × 10 1 ; 10, 1 × 10 1 .

    Journal: Journal of Clinical Microbiology

    Article Title: Efficient Extraction of Viral DNA and Viral RNA by the Chemagic Viral DNA/RNA Kit Allows Sensitive Detection of Cytomegalovirus, Hepatitis B Virus, and Hepatitis G Virus by PCR

    doi: 10.1128/JCM.41.11.5273-5276.2003

    Figure Lengend Snippet: Amplification of a serial dilution of the EUROHEP standard with HBV-specific primers in a nested PCR following extraction with the Chemagic DNA/RNA kit and the QIAamp DNA Blood Mini kit. (A) Chemagen extracts. (B) QIAGEN extracts. Lane M shows a 100-bp ladder, and lane C shows results for the negative control. Lanes 1 through 10 show results for different viral loads expressed in numbers of copies per ml: 1, 4 × 10 5 ; 2, 4 × 10 4 ; 3, 4 × 10 3 ; 4, 4 × 10 2 ; 5, 2 × 10 2 ; 6, 1 × 10 2 ; 7, 8 × 10 1 ; 8, 4 × 10 1 ; 9, 2 × 10 1 ; 10, 1 × 10 1 .

    Article Snippet: Identical results were obtained after extraction of the identical specimens with the QIAamp Viral RNA Mini kit.

    Techniques: Amplification, Serial Dilution, Nested PCR, Negative Control

    Amplification of a serial dilution of the standard plasmid pCR-gpB by real-time PCR with CMV-specific primers (amplicon, 254 bp) on the LightCycler instrument (software, version 3.5; analysis method, fit points with manual noise band adjustment, hybridization probe format) following extraction with the Chemagic DNA/RNA kit and the QIAamp DNA Blood Mini kit. pCR-gpB concentrations (from left to right) were 2 × 10 5 , 2 × 10 4 , 2 × 10 3 , and 2 × 10 2 copies per ml of serum. Continuous line, Chemagen extracts; broken line, QIAGEN extracts. Cycle number, cycle number of the amplification reaction; F2/F1, quotient of fluorescence channel F2 (hybridization probe) and fluorescence channel F1 (fluorescein).

    Journal: Journal of Clinical Microbiology

    Article Title: Efficient Extraction of Viral DNA and Viral RNA by the Chemagic Viral DNA/RNA Kit Allows Sensitive Detection of Cytomegalovirus, Hepatitis B Virus, and Hepatitis G Virus by PCR

    doi: 10.1128/JCM.41.11.5273-5276.2003

    Figure Lengend Snippet: Amplification of a serial dilution of the standard plasmid pCR-gpB by real-time PCR with CMV-specific primers (amplicon, 254 bp) on the LightCycler instrument (software, version 3.5; analysis method, fit points with manual noise band adjustment, hybridization probe format) following extraction with the Chemagic DNA/RNA kit and the QIAamp DNA Blood Mini kit. pCR-gpB concentrations (from left to right) were 2 × 10 5 , 2 × 10 4 , 2 × 10 3 , and 2 × 10 2 copies per ml of serum. Continuous line, Chemagen extracts; broken line, QIAGEN extracts. Cycle number, cycle number of the amplification reaction; F2/F1, quotient of fluorescence channel F2 (hybridization probe) and fluorescence channel F1 (fluorescein).

    Article Snippet: Identical results were obtained after extraction of the identical specimens with the QIAamp Viral RNA Mini kit.

    Techniques: Amplification, Serial Dilution, Plasmid Preparation, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Software, Hybridization, Fluorescence

    Integrated assays using plasma spiked with Armored RNA HIV. Armored RNA HIV was spiked into human plasma at concentrations ranging from 10,000 copies/mL to 1,000 copies/mL amd extracted with a QIAamp® Viral RNA Mini Kit according to the manufacturer’s

    Journal: The Journal of infectious diseases

    Article Title: Nucleic acid assay system for tier II labs and moderately complex clinics to detect HIV in low-resource settings

    doi: 10.1086/650388

    Figure Lengend Snippet: Integrated assays using plasma spiked with Armored RNA HIV. Armored RNA HIV was spiked into human plasma at concentrations ranging from 10,000 copies/mL to 1,000 copies/mL amd extracted with a QIAamp® Viral RNA Mini Kit according to the manufacturer’s

    Article Snippet: Note: according to the QIAamp® Viral RNA Mini Kit Handbook, this Kit can also be performed using the QIAvac® 24 Plus (Qiagen, Valencia, CA) if a centrifuge is not available.

    Techniques:

    SARS-CoV-2 RNA can be detected from COVID-19 patient nasopharyngeal swabs by RT-qPCR without an RNA extraction step ( a ) Nasopharyngeal (NP) swab diluents from two confirmed COVID-19 patients were pooled, and using the 2019-nCoV_N3 primer/probe set, the mixture was either i) subjected to RNA extraction using the Qiagen QIAamp Viral RNA Mini kit followed by subsequent testing by RT-qPCR (using the equivalent of 11.3 ul swab diluent) or ii) directly added to the RT-qPCR reaction, with or without a preheating step (five minutes at 70°C, “NP sample + heat”). As a control, the indicated quantities of the CDC 2019-nCoV Positive Control SARS-CoV-2 synthetic RNA was spiked into M6 transport media, purified using the QIAamp Viral RNA Mini kit, and screened by RT-qPCR. NP swab samples from seven additional donors were screened by direct RT-qPCR for SARS-CoV-2 RNA using ( b ) the 2019-nCoV_N1 primer/probe set, ( c ) the 2019-nCoV_N2 primer/probe set, or ( d ) for human RNase P RNA using the RP primer probe set. NP swab samples from donors 1 – 4 were previously shown to contain SARS-CoV-2 RNA by standard clinical RT-qPCR, while donors 5 – 7 were negative. For each primer/probe set, 7 µL ( a ) or 3 µL ( b, c, d ) of NP swab diluent was tested in the RT-qPCR reaction per donor. For the N1 and N2 primer probe sets, the fully synthetic SARS-CoV-2 RNA Control 2 from Twist Bioscience was loaded at serial 10-fold dilutions (A, 3×10 6 copies; B, 3×10 5 copies; C, 3×10 4 copies; D, 3×10 3 copies; E, 3×10 2 copies; F, 3×10 1 copies) as indicated in panels b and c . No template control (NTC) wells were included for each primer/probe set and each was negative. For panels b and c , the correlation coefficients (R 2 ) of the standard curves were 0.999 and 0.995, respectively. The dashed line at cycle 40 in each graph indicates the limit of detection.

    Journal: bioRxiv

    Article Title: DIRECT RT-qPCR DETECTION OF SARS-CoV-2 RNA FROM PATIENT NASOPHARYNGEAL SWABS WITHOUT AN RNA EXTRACTION STEP

    doi: 10.1101/2020.03.20.001008

    Figure Lengend Snippet: SARS-CoV-2 RNA can be detected from COVID-19 patient nasopharyngeal swabs by RT-qPCR without an RNA extraction step ( a ) Nasopharyngeal (NP) swab diluents from two confirmed COVID-19 patients were pooled, and using the 2019-nCoV_N3 primer/probe set, the mixture was either i) subjected to RNA extraction using the Qiagen QIAamp Viral RNA Mini kit followed by subsequent testing by RT-qPCR (using the equivalent of 11.3 ul swab diluent) or ii) directly added to the RT-qPCR reaction, with or without a preheating step (five minutes at 70°C, “NP sample + heat”). As a control, the indicated quantities of the CDC 2019-nCoV Positive Control SARS-CoV-2 synthetic RNA was spiked into M6 transport media, purified using the QIAamp Viral RNA Mini kit, and screened by RT-qPCR. NP swab samples from seven additional donors were screened by direct RT-qPCR for SARS-CoV-2 RNA using ( b ) the 2019-nCoV_N1 primer/probe set, ( c ) the 2019-nCoV_N2 primer/probe set, or ( d ) for human RNase P RNA using the RP primer probe set. NP swab samples from donors 1 – 4 were previously shown to contain SARS-CoV-2 RNA by standard clinical RT-qPCR, while donors 5 – 7 were negative. For each primer/probe set, 7 µL ( a ) or 3 µL ( b, c, d ) of NP swab diluent was tested in the RT-qPCR reaction per donor. For the N1 and N2 primer probe sets, the fully synthetic SARS-CoV-2 RNA Control 2 from Twist Bioscience was loaded at serial 10-fold dilutions (A, 3×10 6 copies; B, 3×10 5 copies; C, 3×10 4 copies; D, 3×10 3 copies; E, 3×10 2 copies; F, 3×10 1 copies) as indicated in panels b and c . No template control (NTC) wells were included for each primer/probe set and each was negative. For panels b and c , the correlation coefficients (R 2 ) of the standard curves were 0.999 and 0.995, respectively. The dashed line at cycle 40 in each graph indicates the limit of detection.

    Article Snippet: University of Vermont To compare the effect of nucleic acid extraction to direct RT-PCR, RNA from a pooled patient sample was extracted using the QIAamp Viral RNA Mini (Qiagen, Cat. No. 52904) kit according to the manufacturer’s instructions.

    Techniques: Quantitative RT-PCR, RNA Extraction, Positive Control, Purification