Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: Transcriptomic data from myxobacteria exposed to C6-AHL. A. Differentially expressed genes and features from M. xanthus exposed to C6-AHL when compared with signal unexposed M. xanthus control ( p ≤ 0.05); * indicates features also impacted at p ≤ 0.01. B. Differentially expressed genes from C. ferrugineus exposed to C6-AHL when compared with signal unexposed C. ferrugineus control ( p ≤ 0.01). Data depicted as an average log 2 fold change from three biological replicates. Impacted features annotated as hypothetical not included.

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: Putative roles of Prokaryotic Genome Annotation Pipeline (PGAP)-annotated genes impacted by C6-AHL exposure (from ) comparing M. xanthus and C. ferrugineus .

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: Transcriptomic data from myxobacteria exposed to HHQ. A. Differentially expressed genes and features from M. xanthus exposed to HHQ when compared with signal unexposed M. xanthus control ( p ≤ 0.05). B. Differentially expressed genes from C. ferrugineus exposed to HHQ when compared with signal unexposed C. ferrugineus control ( p ≤ 0.05). Data depicted as an average log 2 fold change from three biological replicates. Impacted features annotated as hypothetical not included.

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: Putative roles of PGAP-annotated genes impacted by HHQ exposure (from ) comparing M. xanthus and C. ferrugineus .

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: Comparison of metabolomic response to C6-AHL, 3-oxo-C6-AHL and HHQ exposure experiments with M. xanthus (A and B) and C. ferrugineus (C and D). Numbers included in each Venn diagram account for a unique detected feature with a significantly impacted intensity upon exposure to the indicated signalling molecule provided by XCMS-multigroup analysis ( n = 3, p ≤ 0.02).

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: Comparison of metabolomic response to C6-AHL, 3-oxo-C6-AHL, and HHQ exposure experiments with M. xanthus (A and B) and C. ferrugineus (C and D) including additional C6-AHL + HHQ exposure experiments. Numbers included in each Venn diagram account for a unique detected feature with a significantly impacted intensity upon exposure to the indicated signalling molecule provided by XCMS-multigroup analysis ( n = 3, p ≤ 0.02).

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: A. Extracted ion chromatograph (EIC) depicting presence of HQNO and PQS in HHQ exposed extracts from C. ferrugineus and not observed in HHQ exposed extracts from M. xanthus . B. EIC depicting presence of PQS-NO in HHQ exposed extracts of C. ferrugineus , also not present in M. xanthus extracts. Chromatographs rendered with MZmine v2.37. C. Oxidative detoxification of HHQ by C. ferrugineus including exact mass values from ChemDraw Professional v17.1. D. Detected ion intensities for PQS-NO comparing crude extracts of C. ferrugineus exposed to HHQ, PQS and HQNO; detected intensity data provided by XCMS-multigroup analysis ( n = 3; p ≤ 0.02).

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques:

Journal: Environmental microbiology

Article Title: Differential response to prey quorum signals indicates predatory specialization of myxobacteria and ability to predate Pseudomonas aeruginosa

doi: 10.1111/1462-2920.15812

Figure Lengend Snippet: A. Lawn culture predation assay data depicting superior predation of P. aeruginosa by C. ferrugineus ( n = 3; p ≤ 0.005). Statistical significance calculated using an unpaired t test with Welch’s correction. B. Swarming expansion assay data depicting HHQ inhibition of M. xanthus swarming ( n = 4) with significant differences in swarm diameters ( p < 0.001) observed between HHQ exposed and unexposed data after day 3. C. Swarming expansion assay data depicting no significant inhibition of C. ferrugineus swarming ( n = 4) during HHQ exposure conditions. Statistical significance for swarming assays was determined using two-way ANOVA and Tukey’s multiple comparisons test.

Article Snippet: Cystobacter ferrugineus strain Cbfe23, DSM 52764, initially obtained from German Collection of Microorganisms (DSMZ) in Braunschweig, and Myxococcus xanthus strain GJV1 were employed in this study.

Techniques: Inhibition

Journal: bioRxiv

Article Title: The ubiquitous terpene geosmin is a warning chemical

doi: 10.1101/2021.03.09.434661

Figure Lengend Snippet: A, Chemical structures of geosmin and 2-methylisoborneol. B, Growth curve and geosmin production of M. xanthus DK1622 in 1% CTT. Geosmin concentrations were determined via GC-MS, with the aid of a calibration curve derived from solutions of authentic geosmin. † Cells began to clump at day 7, complicating OD 600 measurements. C, Viability of adult C. elegans hermaphrodites in the presence of geosmin, 2-methylisoborneol and bleach. Worms non-responsive to touch stimuli were declared dead. Experiments were conducted in triplicate with five adult C. elegans per well (n=15). D, Comparison of the movement of adult C. elegans hermaphrodites on NGM and NGM-geosmin (0.54 μg/mL) plates, quantified with Imaris (track line) and WormLab (peristaltic speed, periodicity). Significant differences between NGM and NGM-geosmin plates are represented by a red box ( P < 0.05) and non-significant differences by a green box ( P ≥ 0.05). Experiments were conducted in triplicate with three worms per plate (n=9). Full details are available in External Data Table 4.

Article Snippet: Myxococcus xanthus DK1622, Micrococcus luteus DSM 20030 and Bacillus subtilis DSM 10 were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures.

Techniques: Gas Chromatography-Mass Spectrometry, Derivative Assay

Journal: bioRxiv

Article Title: The ubiquitous terpene geosmin is a warning chemical

doi: 10.1101/2021.03.09.434661

Figure Lengend Snippet: A, Proportion of adult C. elegans N2 worms in colonies of S. coelicolor M145 (WT), J3003 ( ΔgeoA ) and J2192 ( ΔgeoA ΔmibAB ) as a function of time. B, Proportion of C. elegans PR674 ( che-1(p674) , ASE deficient) worms in colonies of S. coelicolor M145 (WT), J3003 ( ΔgeoA ) and J2192 ( ΔgeoA ΔmibAB ) as a function of time. C, Proportion of C. elegans N2 worms in colonies of S. coelicolor J2912 that were pre-treated with geosmin, 2-methylisoborneol, or distilled, deionized water as a function of time. D, Proportion of C. elegans N2 and PR674 worms in colonies of M. xanthus DK1622 as a function of time. E, Consumption of S. coelicolor by C. elegans . Blue arrows indicate the bacterial colony, the red arrow indicates the presence of bacteria in the C. elegans pharynx. F, Production of spores and actinorhodin by S. coelicolor in the absence (left) or presence (right) of C. elegans . 10 day cultures, room temperature. G, C. elegans on a M. xanthus lawn. Worms became translucent prior to death and were ultimately digested by the bacteria. All nematode experiments were run in triplicate, with ten adult hermaphrodites per study (n=30). Worms were grown on E. coli OP50 and starved for 20 minutes prior to addition to S. coelicolor or M. xanthus . Statistically significant deviations from J2192 (A, B), H 2 O (C), or wildtype (D) are indicated by * (P < 0.05) or ** (P<0.01). Representative images are shown.

Article Snippet: Myxococcus xanthus DK1622, Micrococcus luteus DSM 20030 and Bacillus subtilis DSM 10 were obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures.

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: Structural characterization of Myxococcus xanthus MglC, a component of the polarity control system, and its interactions with its paralog MglB

doi: 10.1016/j.jbc.2021.100308

Figure Lengend Snippet: Comparative structural analysis of MglC with other RLC7 family proteins. A , structural superposition of MglC in orange with other proteins of RLC7 family LAMTOR2 (PDB ID: 5Y3A, blue ), and MglB from Myxococcus xanthus , (PDB ID: 6H5B, pink ). B , structural comparison of MglC showing difference in relative orientation of α2 compared with other RLC7 family proteins. MglB, M utual gl iding-motility protein B ; MglC, M utual gl iding motility protein C ; RLC7, R egulatory L ight C hain 7.

Article Snippet: The mglC, mglB and mglB ΔCT genes were amplified from genomic DNA of M. xanthus (DSMZ, catalog number 16526) using primers mglC-F (5′-GCTAGTCGCTAGCTCCTTCCGCACGCACCTCGAG-3′), mglC-R (5′-GCTAAAGCTTCTAGAGCTCGGCGCGCACCT-3′), mglB-F (5′-GCTGAAGCTAGCATGGGCACGCAACTGG-3′), mglB-R (5′-CGTAAAGCTTTTACTCGCTGAAGAGGTTGTCG-3′), mglB ΔCT -R (5′-CGTAAAGCTTTTACACCAGGCTCTCGAAGATCTTCGTGAGCTC-3′) synthesized by Sigma-Aldrich.

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: Structural characterization of Myxococcus xanthus MglC, a component of the polarity control system, and its interactions with its paralog MglB

doi: 10.1016/j.jbc.2021.100308

Figure Lengend Snippet: Model for regulation of polarity reversal in Myxococcus xanthus . According to our proposed model, RomR recruits MglC to poles in an asymmetric manner, and at the lagging pole, MglC binds MglB in 2:4 stoichiometry, whereas MglA is present at the leading pole. The polarity reversal starts as the MglA and MglB relocate followed by RomRX and MglC. MglA, M utual gl iding-motility protein A ; MglB, M utual gl iding-motility protein B ; MglC, M utual gl iding motility protein C ; RomRX, R equired f o r m otility response regulator complex.

Article Snippet: The mglC, mglB and mglB ΔCT genes were amplified from genomic DNA of M. xanthus (DSMZ, catalog number 16526) using primers mglC-F (5′-GCTAGTCGCTAGCTCCTTCCGCACGCACCTCGAG-3′), mglC-R (5′-GCTAAAGCTTCTAGAGCTCGGCGCGCACCT-3′), mglB-F (5′-GCTGAAGCTAGCATGGGCACGCAACTGG-3′), mglB-R (5′-CGTAAAGCTTTTACTCGCTGAAGAGGTTGTCG-3′), mglB ΔCT -R (5′-CGTAAAGCTTTTACACCAGGCTCTCGAAGATCTTCGTGAGCTC-3′) synthesized by Sigma-Aldrich.

Techniques:

Journal: International Journal of Environmental Research and Public Health

Article Title: Enhancement of Contact Lens Disinfection by Combining Disinfectant with Visible Light Irradiation

doi: 10.3390/ijerph17176422

Figure Lengend Snippet: Demonstration of different agar plate appearances ( a ) with fragmentary lawn through excessive light dose of 280 J/cm 2 ( I ), indefinite inhibition zone with 130 mg/10 mL agar concentration and 100% ReNu Multiplus ( II ), optimal appearance with different concentrations of AOSept ( III ). Diameter of inhibition zones on P. fluorescens lawn achieved with different concentrations of hydrogen peroxide solution AOSept, dependent on the dose of 405 nm applied with 20 mW/cm 2 ( b ). Error bars indicate the deviation in the three experiments. The red dotted line indicates the size of inhibition zones generated with 100% AOSept without irradiation.

Article Snippet: Pseudomonas fluorescens (DSM4358), E. coli (DSM1607) and S. carnosus (DSM20501) were obtained from DSMZ (Deutsche Sammlung für Mikroorganismen und Zellkulturen, Braunschweig, Germany).

Techniques: Inhibition, Concentration Assay, Generated, Irradiation

Journal: International Journal of Environmental Research and Public Health

Article Title: Enhancement of Contact Lens Disinfection by Combining Disinfectant with Visible Light Irradiation

doi: 10.3390/ijerph17176422

Figure Lengend Snippet: Reduction results with nutrient pads for all combinations tested including single approaches as reference. Error bars indicate the deviation in the three experiments ( a ), Example of “synergy“ calculation as enhancement of the combination over the sum of ReNu Multiplus and light applied separately, and progress of these “synergies“ for different ReNu Multiplus concentrations on P. fluorescens ( b ).

Article Snippet: Pseudomonas fluorescens (DSM4358), E. coli (DSM1607) and S. carnosus (DSM20501) were obtained from DSMZ (Deutsche Sammlung für Mikroorganismen und Zellkulturen, Braunschweig, Germany).

Techniques:

Journal: International Journal of Environmental Research and Public Health

Article Title: Enhancement of Contact Lens Disinfection by Combining Disinfectant with Visible Light Irradiation

doi: 10.3390/ijerph17176422

Figure Lengend Snippet: Reference experiments on agar plates to achieve dose-effect curves for single approaches on P. fluorescens : 405 nm irradiation in phosphate buffered saline (PBS) with 10 mW/cm 2 ( a ), 20 mW/cm 2 ( b ) and 40 mW/cm 2 ( c ) as well as ReNu Multiplus exposure for 1, 2, and 4 h at different concentrations ( d ). Error bars indicate the deviation in the two experiments.

Article Snippet: Pseudomonas fluorescens (DSM4358), E. coli (DSM1607) and S. carnosus (DSM20501) were obtained from DSMZ (Deutsche Sammlung für Mikroorganismen und Zellkulturen, Braunschweig, Germany).

Techniques: Irradiation

Journal: International Journal of Environmental Research and Public Health

Article Title: Enhancement of Contact Lens Disinfection by Combining Disinfectant with Visible Light Irradiation

doi: 10.3390/ijerph17176422

Figure Lengend Snippet: Combination Index values calculated based on Loewe Additivity for log reduction results on agar plates achieved with ReNu Multiplus and 405 nm on P. fluorescens .

Article Snippet: Pseudomonas fluorescens (DSM4358), E. coli (DSM1607) and S. carnosus (DSM20501) were obtained from DSMZ (Deutsche Sammlung für Mikroorganismen und Zellkulturen, Braunschweig, Germany).

Techniques:

Journal: International Journal of Environmental Research and Public Health

Article Title: Enhancement of Contact Lens Disinfection by Combining Disinfectant with Visible Light Irradiation

doi: 10.3390/ijerph17176422

Figure Lengend Snippet: Effectiveness of undiluted ReNu Multiplus disinfection solution (100%) against P. fluorescens at different bacterial concentrations, tested with agar plates. Error bars indicate the deviation in the three experiments ( a ). Comparison of log results with nutrient pads and agar plates for combination of ReNu Multiplus and visible 405 nm light at 20 mW/cm 2 for 140 J/cm 2 . Error bars indicate the deviation in the three experiments ( b ).

Article Snippet: Pseudomonas fluorescens (DSM4358), E. coli (DSM1607) and S. carnosus (DSM20501) were obtained from DSMZ (Deutsche Sammlung für Mikroorganismen und Zellkulturen, Braunschweig, Germany).

Techniques:

Journal: International Journal of Environmental Research and Public Health

Article Title: Enhancement of Contact Lens Disinfection by Combining Disinfectant with Visible Light Irradiation

doi: 10.3390/ijerph17176422

Figure Lengend Snippet: Log reductions determined from growth delay analysis for different intensities of 405 nm light and different concentrations of disinfection solutions: ReNu Multiplus on P. fluorescens ( a ), OptiFree Express on P. fluorescens ( b ), ReNu Multiplus on E. coli ( c ) and ReNu Multiplus on S. carnosus ( d ). Upper part of diagram presenting results for single approaches, lower part presenting combination results (+). Error bars indicate the deviation in the three experiments.

Article Snippet: Pseudomonas fluorescens (DSM4358), E. coli (DSM1607) and S. carnosus (DSM20501) were obtained from DSMZ (Deutsche Sammlung für Mikroorganismen und Zellkulturen, Braunschweig, Germany).

Techniques: