truxtrac ffpe tna plus  (Covaris)


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    Name:
    truXTRAC FFPE total NA Plus Kit
    Description:
    Optimized to work with larger FFPE inputs 45 μm of FFPE sections Kit contains reagents and consumables required for extraction and purification of DNA and RNA from 25 FFPE tissue samples
    Catalog Number:
    520252
    Price:
    None
    Category:
    Reagent
    Applications:
    Total Nucleic Acid Extraction from FFPE
    Quantity:
    25 0
    Buy from Supplier


    Structured Review

    Covaris truxtrac ffpe tna plus
    truXTRAC FFPE total NA Plus Kit
    Optimized to work with larger FFPE inputs 45 μm of FFPE sections Kit contains reagents and consumables required for extraction and purification of DNA and RNA from 25 FFPE tissue samples
    https://www.bioz.com/result/truxtrac ffpe tna plus/product/Covaris
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    truxtrac ffpe tna plus - by Bioz Stars, 2021-05
    94/100 stars

    Images

    1) Product Images from "Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samples"

    Article Title: Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samples

    Journal: bioRxiv

    doi: 10.1101/2020.06.02.122150

    Nucleic acid extraction quantitation and quality assessment RNA and/or DNA extracted by either the Covaris truXTRAC FFPE tNA Plus kit (red) or the QIAGEN FFPE miRNeasy kit (blue) were evaluated by several measures. (A) RNA was quantified and summarized by the total amount (ug) extracted across triplicates (Covaris) or three elutions (QIAGEN). This value was also summarized by the amount of nucleic acid extracted per micron (um) of tissue. (B) RNA quality was assessed for purity (260:280 ratio) and quality (RNA integrity number [RIN], DV200). Error bars represent the range of values across Covaris replicates (n=3) or the three elutions from the QIAGEN protocol. (C) DNA quantity was summarized by the total amount (ug) extracted across triplicates in the Covaris protocol and the amount per micron of tissue extracted. The DNA integrity number (DIN) was used to summarize quality.
    Figure Legend Snippet: Nucleic acid extraction quantitation and quality assessment RNA and/or DNA extracted by either the Covaris truXTRAC FFPE tNA Plus kit (red) or the QIAGEN FFPE miRNeasy kit (blue) were evaluated by several measures. (A) RNA was quantified and summarized by the total amount (ug) extracted across triplicates (Covaris) or three elutions (QIAGEN). This value was also summarized by the amount of nucleic acid extracted per micron (um) of tissue. (B) RNA quality was assessed for purity (260:280 ratio) and quality (RNA integrity number [RIN], DV200). Error bars represent the range of values across Covaris replicates (n=3) or the three elutions from the QIAGEN protocol. (C) DNA quantity was summarized by the total amount (ug) extracted across triplicates in the Covaris protocol and the amount per micron of tissue extracted. The DNA integrity number (DIN) was used to summarize quality.

    Techniques Used: Quantitation Assay, Formalin-fixed Paraffin-Embedded

    2) Product Images from "Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samples"

    Article Title: Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samples

    Journal: bioRxiv

    doi: 10.1101/2020.06.02.122150

    Nucleic acid extraction quantitation and quality assessment RNA and/or DNA extracted by either the Covaris truXTRAC FFPE tNA Plus kit (red) or the QIAGEN FFPE miRNeasy kit (blue) were evaluated by several measures. (A) RNA was quantified and summarized by the total amount (ug) extracted across triplicates (Covaris) or three elutions (QIAGEN). This value was also summarized by the amount of nucleic acid extracted per micron (um) of tissue. (B) RNA quality was assessed for purity (260:280 ratio) and quality (RNA integrity number [RIN], DV200). Error bars represent the range of values across Covaris replicates (n=3) or the three elutions from the QIAGEN protocol. (C) DNA quantity was summarized by the total amount (ug) extracted across triplicates in the Covaris protocol and the amount per micron of tissue extracted. The DNA integrity number (DIN) was used to summarize quality.
    Figure Legend Snippet: Nucleic acid extraction quantitation and quality assessment RNA and/or DNA extracted by either the Covaris truXTRAC FFPE tNA Plus kit (red) or the QIAGEN FFPE miRNeasy kit (blue) were evaluated by several measures. (A) RNA was quantified and summarized by the total amount (ug) extracted across triplicates (Covaris) or three elutions (QIAGEN). This value was also summarized by the amount of nucleic acid extracted per micron (um) of tissue. (B) RNA quality was assessed for purity (260:280 ratio) and quality (RNA integrity number [RIN], DV200). Error bars represent the range of values across Covaris replicates (n=3) or the three elutions from the QIAGEN protocol. (C) DNA quantity was summarized by the total amount (ug) extracted across triplicates in the Covaris protocol and the amount per micron of tissue extracted. The DNA integrity number (DIN) was used to summarize quality.

    Techniques Used: Quantitation Assay, Formalin-fixed Paraffin-Embedded

    3) Product Images from "Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samples"

    Article Title: Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samples

    Journal: bioRxiv

    doi: 10.1101/2020.06.02.122150

    Nucleic acid extraction quantitation and quality assessment RNA and/or DNA extracted by either the Covaris truXTRAC FFPE tNA Plus kit (red) or the QIAGEN FFPE miRNeasy kit (blue) were evaluated by several measures. (A) RNA was quantified and summarized by the total amount (ug) extracted across triplicates (Covaris) or three elutions (QIAGEN). This value was also summarized by the amount of nucleic acid extracted per micron (um) of tissue. (B) RNA quality was assessed for purity (260:280 ratio) and quality (RNA integrity number [RIN], DV200). Error bars represent the range of values across Covaris replicates (n=3) or the three elutions from the QIAGEN protocol. (C) DNA quantity was summarized by the total amount (ug) extracted across triplicates in the Covaris protocol and the amount per micron of tissue extracted. The DNA integrity number (DIN) was used to summarize quality.
    Figure Legend Snippet: Nucleic acid extraction quantitation and quality assessment RNA and/or DNA extracted by either the Covaris truXTRAC FFPE tNA Plus kit (red) or the QIAGEN FFPE miRNeasy kit (blue) were evaluated by several measures. (A) RNA was quantified and summarized by the total amount (ug) extracted across triplicates (Covaris) or three elutions (QIAGEN). This value was also summarized by the amount of nucleic acid extracted per micron (um) of tissue. (B) RNA quality was assessed for purity (260:280 ratio) and quality (RNA integrity number [RIN], DV200). Error bars represent the range of values across Covaris replicates (n=3) or the three elutions from the QIAGEN protocol. (C) DNA quantity was summarized by the total amount (ug) extracted across triplicates in the Covaris protocol and the amount per micron of tissue extracted. The DNA integrity number (DIN) was used to summarize quality.

    Techniques Used: Quantitation Assay, Formalin-fixed Paraffin-Embedded

    Related Articles

    RNA Sequencing Assay:

    Article Title: Perspectives in immunotherapy: meeting report from the “Immunotherapy Bridge” (December 4th–5th, 2019, Naples, Italy)
    Article Snippet: Formalin-fixed paraffin-embedded (FFPE) preservation is often used because of its economic feasibility but can compromise both the quality and quantity of nucleic acid extracted from samples. .. In an experimental comparison to evaluate nucleic acid extraction and various RNA sequencing library preparations from FFPE samples, the Covaris truXTRAC® FFPE tNA Plus kit showed superior RNA yields and quality from less input tissue compared to the Qiagen miRNeasy® FFPE kit and enables concurrent dual DNA and RNA extraction from the same lesion. .. Optimizing library preparation for RNA sequencing was achieved with the Agilent SureSelectXT HS technology that promoted amplification and capture of longer RNA fragments.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Perspectives in immunotherapy: meeting report from the “Immunotherapy Bridge” (December 4th–5th, 2019, Naples, Italy)
    Article Snippet: Formalin-fixed paraffin-embedded (FFPE) preservation is often used because of its economic feasibility but can compromise both the quality and quantity of nucleic acid extracted from samples. .. In an experimental comparison to evaluate nucleic acid extraction and various RNA sequencing library preparations from FFPE samples, the Covaris truXTRAC® FFPE tNA Plus kit showed superior RNA yields and quality from less input tissue compared to the Qiagen miRNeasy® FFPE kit and enables concurrent dual DNA and RNA extraction from the same lesion. .. Optimizing library preparation for RNA sequencing was achieved with the Agilent SureSelectXT HS technology that promoted amplification and capture of longer RNA fragments.

    Article Title: Characterization of the immune microenvironment in brain metastases from different solid tumors, et al. Characterization of the immune microenvironment in brain metastases from different solid tumors
    Article Snippet: .. The analytical validation of this panel was reported as previous described., In detail, RNA was extracted from FFPE slides using the truXTRAC FFPE extraction kit (Covaris), according to manufacturer's recommendation. .. After purification, RNA yield was quantified using Qubit™ RNA HS Assay Kit (Thermo Fisher Scientific).

    Article Title: Development and analytical validation of a next-generation sequencing based microsatellite instability (MSI) assay
    Article Snippet: For level of detection studies, non-malignant tissue was also identified for isolation and reviewed to exclude any neoplastic tissue. .. Genomic DNA was extracted from areas identified by the pathologist using 3–5 unstained slides with the truXTRAC FFPE extraction kit (Covaris, Inc., Woburn, MA, USA), as described previously. .. DNA was eluted in 100 µL water, and yield was determined by the Qubit DNA HS Assay (Thermo Fisher Scientific, Waltham, MA, USA), as per manufacturer’s recommendation.

    Article Title: Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samples
    Article Snippet: These studies were performed on an FFPE-preserved patient tumor biopsy (PT0017bx), to simulate a clinically relevant scenario, with technical replicates. .. Both the Covaris truXTRAC FFPE tNA Plus and QIAGEN miRNeasy FFPE protocols successfully extracted RNA from FFPE-preserved tissue. .. However, the Covaris protocol demonstrated increased consistency in control samples, higher yields of RNA, and increased average length of RNA molecules.

    Article Title: Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samples
    Article Snippet: Thus, to verify that the increased extraction quantities and qualities were not an outlier in the PT0017bx sample, extractions were performed from two additional FFPE samples (kidney and liver tissue, each in triplicate). .. RNA extracted using the Covaris truXTRAC FFPE tNA Plus kit showed higher yield, increased DV200 values, and increased yield of RNA at least 200nt in length, compared to the QIAGEN miRNeasy FFPE Kit in both samples ( Supplementary Table S1 , Supplementary Figure S1). .. Furthermore, the variance in quantity and quality of RNA was reduced using the Covaris kit, compared to QIAGEN for both samples, suggesting increased experimental consistency across these metrics associated with the Covaris-extracted samples.

    Article Title: Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samples
    Article Snippet: Each elution was preserved individually, to maximize the amount of RNA isolated and assess the added benefit of collecting multiple elutions. .. DNA and RNA extraction using the Covaris truXTRAC FFPE tNA Plus (Column) kit, according to the manufacturer’s protocol, specifically on the Covaris E220 evolution platform. .. Of note, no changes were made to the protocol; specifically, the isopropanol concentration (31.1%) was not altered to impact RNA yield or quality.

    Article Title: Mutational profile of primary breast diffuse large B-cell lymphoma
    Article Snippet: The probes for this custom panel were designed with DesignStudio (Illumina) and consisted of 1399 amplicons with an average size of 175 bp and a cumulative targeted region of 140 kb. .. DNA was extracted from formalin fixed and paraffin embedded tissues (FFPET) with truXTRACT FFPE DNA kit (Covaris, Woburn MA USA). ..

    RNA Extraction:

    Article Title: Perspectives in immunotherapy: meeting report from the “Immunotherapy Bridge” (December 4th–5th, 2019, Naples, Italy)
    Article Snippet: Formalin-fixed paraffin-embedded (FFPE) preservation is often used because of its economic feasibility but can compromise both the quality and quantity of nucleic acid extracted from samples. .. In an experimental comparison to evaluate nucleic acid extraction and various RNA sequencing library preparations from FFPE samples, the Covaris truXTRAC® FFPE tNA Plus kit showed superior RNA yields and quality from less input tissue compared to the Qiagen miRNeasy® FFPE kit and enables concurrent dual DNA and RNA extraction from the same lesion. .. Optimizing library preparation for RNA sequencing was achieved with the Agilent SureSelectXT HS technology that promoted amplification and capture of longer RNA fragments.

    Article Title: Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samples
    Article Snippet: Each elution was preserved individually, to maximize the amount of RNA isolated and assess the added benefit of collecting multiple elutions. .. DNA and RNA extraction using the Covaris truXTRAC FFPE tNA Plus (Column) kit, according to the manufacturer’s protocol, specifically on the Covaris E220 evolution platform. .. Of note, no changes were made to the protocol; specifically, the isopropanol concentration (31.1%) was not altered to impact RNA yield or quality.

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  • 94
    Covaris truxtrac ffpe tna plus
    Nucleic acid extraction quantitation and quality assessment RNA and/or DNA extracted by either the Covaris <t>truXTRAC</t> <t>FFPE</t> <t>tNA</t> Plus kit (red) or the QIAGEN FFPE miRNeasy kit (blue) were evaluated by several measures. (A) RNA was quantified and summarized by the total amount (ug) extracted across triplicates (Covaris) or three elutions (QIAGEN). This value was also summarized by the amount of nucleic acid extracted per micron (um) of tissue. (B) RNA quality was assessed for purity (260:280 ratio) and quality (RNA integrity number [RIN], DV200). Error bars represent the range of values across Covaris replicates (n=3) or the three elutions from the QIAGEN protocol. (C) DNA quantity was summarized by the total amount (ug) extracted across triplicates in the Covaris protocol and the amount per micron of tissue extracted. The DNA integrity number (DIN) was used to summarize quality.
    Truxtrac Ffpe Tna Plus, supplied by Covaris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truxtrac ffpe tna plus/product/Covaris
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    truxtrac ffpe tna plus - by Bioz Stars, 2021-05
    94/100 stars
      Buy from Supplier

    Image Search Results


    Nucleic acid extraction quantitation and quality assessment RNA and/or DNA extracted by either the Covaris truXTRAC FFPE tNA Plus kit (red) or the QIAGEN FFPE miRNeasy kit (blue) were evaluated by several measures. (A) RNA was quantified and summarized by the total amount (ug) extracted across triplicates (Covaris) or three elutions (QIAGEN). This value was also summarized by the amount of nucleic acid extracted per micron (um) of tissue. (B) RNA quality was assessed for purity (260:280 ratio) and quality (RNA integrity number [RIN], DV200). Error bars represent the range of values across Covaris replicates (n=3) or the three elutions from the QIAGEN protocol. (C) DNA quantity was summarized by the total amount (ug) extracted across triplicates in the Covaris protocol and the amount per micron of tissue extracted. The DNA integrity number (DIN) was used to summarize quality.

    Journal: bioRxiv

    Article Title: Optimizing nucleic acid extraction and transcriptome evaluation from low-input, fixed clinical samples

    doi: 10.1101/2020.06.02.122150

    Figure Lengend Snippet: Nucleic acid extraction quantitation and quality assessment RNA and/or DNA extracted by either the Covaris truXTRAC FFPE tNA Plus kit (red) or the QIAGEN FFPE miRNeasy kit (blue) were evaluated by several measures. (A) RNA was quantified and summarized by the total amount (ug) extracted across triplicates (Covaris) or three elutions (QIAGEN). This value was also summarized by the amount of nucleic acid extracted per micron (um) of tissue. (B) RNA quality was assessed for purity (260:280 ratio) and quality (RNA integrity number [RIN], DV200). Error bars represent the range of values across Covaris replicates (n=3) or the three elutions from the QIAGEN protocol. (C) DNA quantity was summarized by the total amount (ug) extracted across triplicates in the Covaris protocol and the amount per micron of tissue extracted. The DNA integrity number (DIN) was used to summarize quality.

    Article Snippet: Both the Covaris truXTRAC FFPE tNA Plus and QIAGEN miRNeasy FFPE protocols successfully extracted RNA from FFPE-preserved tissue.

    Techniques: Quantitation Assay, Formalin-fixed Paraffin-Embedded