truchip chromatin shearing kit with formaldehyde  (Covaris)


Bioz Verified Symbol Covaris is a verified supplier
Bioz Manufacturer Symbol Covaris manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    truChIP Chromatin Shearing Kit with Formaldehyde
    Description:
    The truChIP Chromatin Shearing Kit with Formaldehyde is designed and optimized for the efficient and reproducible shearing of chromatin from cultured mammalian cells suspension or adherent using the Covaris Adaptive Focused Acoustics AFA technology
    Catalog Number:
    520154
    Price:
    None
    Applications:
    Chromatin shearing
    Category:
    Reagent
    Quantity:
    1 0
    Buy from Supplier


    Structured Review

    Covaris truchip chromatin shearing kit with formaldehyde
    truChIP Chromatin Shearing Kit with Formaldehyde
    The truChIP Chromatin Shearing Kit with Formaldehyde is designed and optimized for the efficient and reproducible shearing of chromatin from cultured mammalian cells suspension or adherent using the Covaris Adaptive Focused Acoustics AFA technology
    https://www.bioz.com/result/truchip chromatin shearing kit with formaldehyde/product/Covaris
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    truchip chromatin shearing kit with formaldehyde - by Bioz Stars, 2020-02
    94/100 stars

    Images

    Related Articles

    Centrifugation:

    Article Title: Distinct Requirements of CHD4 during B Cell Development and Antibody Response
    Article Snippet: Cross-linked cells were washed with PBS-based Cell Wash Buffer (PBS, 0.1% FBS, 2mM EDTA) and lysed according to the protocol of truChIP Chromatin Shearing Kit with Formaldehyde (Covaris, 520154). .. Cell lysates were sonicated in the M220 Focused-ultrasonicator (Covaris, 5002295) to shear the chromatin, and debris was removed by centrifugation.

    Agarose Gel Electrophoresis:

    Article Title: Identification of Therapeutic Targets in Rhabdomyosarcoma Through Integrated Genomic, Epigenomic, and Proteomic Analyses
    Article Snippet: Nuclei extraction and chromatin shearing was performed using the TruChIP Chromatin Shearing kit (Covaris 520154) following manufacturer instructions. .. Chromatin shearing efficiency was analyzed using 20 μl of chromatin treated with 0.4 mg/ml proteinase K and placed at 65 °C for 20 min. Sheared chromatin was then run on a 1% agarose gel and Agilent 2200 TapeStation to check for optimal fragment size.

    Isolation:

    Article Title: TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases
    Article Snippet: Chromatin immunoprecipitation Tregs were isolated by magnetic separation from pooled spleens and LNs of Foxp3Thy1.1 mice and CD4+ T cells from CD4Cre ;Tcf7fl/fl mice. .. Collected cells were fixed with freshly prepared 1% methanol-free formaldehyde at room temperature for 5 min. Chromatin shearing was performed using truChIP Chromatin Shearing Kit with Formaldehyde (Covaris) on E220 (Covaris) according to the manufacturer’s manual.

    Article Title: Distinct Requirements of CHD4 during B Cell Development and Antibody Response
    Article Snippet: Chromatin immunoprecipitation Splenic B cells from 8–10 week old mice were isolated and ChIP was performed according to protocol described in the ChIP Assay Kit (Millipore, 17–295). .. Cross-linked cells were washed with PBS-based Cell Wash Buffer (PBS, 0.1% FBS, 2mM EDTA) and lysed according to the protocol of truChIP Chromatin Shearing Kit with Formaldehyde (Covaris, 520154).

    Article Title: PDX1 dynamically regulates pancreatic ductal adenocarcinoma initiation and maintenance
    Article Snippet: .. Chromatin was prepared from isolated acinar cells or cell lines using the truChIP chromatin-shearing reagent kit (Covaris, 520154). .. Chromatin was sheared in 1-mL millitubes (Covaris, 520135) using a Covaris S200 (5% duty cycle, intensity of 4, 200 cycles per burst, for 8 min) to obtain chromatin fragments 100–700 base pairs in length.

    Article Title: Translational Physiology: Persistent pulmonary hypertension alters the epigenetic characteristics of endothelial nitric oxide synthase gene in pulmonary artery endothelial cells in a fetal lamb model
    Article Snippet: .. PAEC chromatin was isolated from six different control and PPHN lambs with the tryChIP chromatin shearing reagent kit (#520154, Covaris) and focused-ultrasonicator M220 (Covaris). .. Chromatin immunoprecipitation (ChIP) was done with antibodies against ERα (sc-7207x; Santa Cruz Biotechnology, Santa Cruz, CA), ERβ (GTX23577; GeneTex, Irvine, CA), Sp1(sc-59x, Santa Cruz Biotechnology), H3K4me3 (cat. #39915; Active Motif, Carlsbad, CA), H3K9ac (cat. #39917, Active Motif), H3K9me3 (cat. #39765, Active Motif), and H4K12ac (cat. #39165, Active Motif) and were performed using EZ-Magna ChIP HT96 kit (cat. #17-10078; EMD Millipore, Temecula, CA).

    Mouse Assay:

    Article Title: TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases
    Article Snippet: Chromatin immunoprecipitation Tregs were isolated by magnetic separation from pooled spleens and LNs of Foxp3Thy1.1 mice and CD4+ T cells from CD4Cre ;Tcf7fl/fl mice. .. Collected cells were fixed with freshly prepared 1% methanol-free formaldehyde at room temperature for 5 min. Chromatin shearing was performed using truChIP Chromatin Shearing Kit with Formaldehyde (Covaris) on E220 (Covaris) according to the manufacturer’s manual.

    Article Title: Distinct Requirements of CHD4 during B Cell Development and Antibody Response
    Article Snippet: Chromatin immunoprecipitation Splenic B cells from 8–10 week old mice were isolated and ChIP was performed according to protocol described in the ChIP Assay Kit (Millipore, 17–295). .. Cross-linked cells were washed with PBS-based Cell Wash Buffer (PBS, 0.1% FBS, 2mM EDTA) and lysed according to the protocol of truChIP Chromatin Shearing Kit with Formaldehyde (Covaris, 520154).

    Real-time Polymerase Chain Reaction:

    Article Title: Translational Physiology: Persistent pulmonary hypertension alters the epigenetic characteristics of endothelial nitric oxide synthase gene in pulmonary artery endothelial cells in a fetal lamb model
    Article Snippet: PAEC chromatin was isolated from six different control and PPHN lambs with the tryChIP chromatin shearing reagent kit (#520154, Covaris) and focused-ultrasonicator M220 (Covaris). .. Real-time PCR was used to quantitate the amount of DNA from eNOS promoter regions of two Sp1 binding sites and three proximal ERE half-sites; 5% of input was used as a loading control ( , ).

    Immunoprecipitation:

    Article Title: Transcription factor activating protein 4 is synthetically lethal and a master regulator of MYCN-amplified neuroblastoma
    Article Snippet: Chromatin immunoprecipitation NGP cells were processed following manufacturer’s instruction using truChIP chromatin shearing kit (Covaris #520154). .. Pre-clearing, incubating with antibodies, and reversal of cross-linking was performed with EZ-ChIP immunoprecipitation kit (Millipore #17-371).

    Article Title: Transcription factor activating protein 4 is synthetically lethal and a master regulator of MYCN-amplified neuroblastoma
    Article Snippet: NGP cells were processed following manufacturer’s instruction using truChIP chromatin shearing kit (Covaris #520154). .. Pre-clearing, incubating with antibodies, and reversal of cross-linking was performed with EZ-ChIP immunoprecipitation kit (Millipore #17-371).

    Incubation:

    Article Title: TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases
    Article Snippet: Collected cells were fixed with freshly prepared 1% methanol-free formaldehyde at room temperature for 5 min. Chromatin shearing was performed using truChIP Chromatin Shearing Kit with Formaldehyde (Covaris) on E220 (Covaris) according to the manufacturer’s manual. .. Briefly, fragmented chromatin was incubated with protein A dynabeads (Invitrogen) pre-coupled with rabbit anti-TCF1 sera at 4°C overnight.

    Article Title: Distinct Requirements of CHD4 during B Cell Development and Antibody Response
    Article Snippet: Cross-linked cells were washed with PBS-based Cell Wash Buffer (PBS, 0.1% FBS, 2mM EDTA) and lysed according to the protocol of truChIP Chromatin Shearing Kit with Formaldehyde (Covaris, 520154). .. Pre-cleared lysate was divided into three parts of 500 μL each and incubated with 1 μg of anti-H3 antibody (Abcam), 2 μg of anti-AID antibody ( ) or control IgG (Jackson Immunoresearch Labs) at 4∘ C overnight.

    Article Title: PDX1 dynamically regulates pancreatic ductal adenocarcinoma initiation and maintenance
    Article Snippet: Chromatin was prepared from isolated acinar cells or cell lines using the truChIP chromatin-shearing reagent kit (Covaris, 520154). .. Fifty micrograms of fragmented chromatin was incubated overnight with 2.5 μL of rabbit anti-PDX1 antibody (Millipore, 07-696).

    Polymerase Chain Reaction:

    Article Title: Translational Physiology: Persistent pulmonary hypertension alters the epigenetic characteristics of endothelial nitric oxide synthase gene in pulmonary artery endothelial cells in a fetal lamb model
    Article Snippet: PAEC chromatin was isolated from six different control and PPHN lambs with the tryChIP chromatin shearing reagent kit (#520154, Covaris) and focused-ultrasonicator M220 (Covaris). .. The PCR primers are listed in .

    Sonication:

    Article Title: EMT is associated with an epigenetic signature of ECM remodeling genes
    Article Snippet: Chromatin immunoprecipitation Chromatin was prepared using the truChIP™ Chromatin Shearing Kit (520154, Covaris, France) according to the manufacturer’s instructions. .. Each sample was submitted to a 8 min sonicated using the M220 Covaris sonicator.

    Article Title: Transcription factor activating protein 4 is synthetically lethal and a master regulator of MYCN-amplified neuroblastoma
    Article Snippet: Chromatin immunoprecipitation NGP cells were processed following manufacturer’s instruction using truChIP chromatin shearing kit (Covaris #520154). .. Chromatin immunoprecipitation NGP cells were processed following manufacturer’s instruction using truChIP chromatin shearing kit (Covaris #520154).

    Article Title: In Vitro Cytokine Licensing Induces Persistent Permissive Chromatin at the IDO1 Promoter
    Article Snippet: Chromatin was cross-linked using truChIP Chromatin Shearing Kit (520154, Covaris, Woburn, MA) according to manufacturer’s instructions, washed twice with cold PBS, and snap-frozen. .. 1.0 mL aliquots were transferred to milliTUBEs (520132, Covaris) and sonicated for 16 minutes at 4–10°C according to manufacturer’s instructions using a Covaris M220 focused ultrasonicator (500295, High Cell Protocol, Covaris) to achieve an average chromatin fragment length of ≤ 700 bp.

    Article Title: Distinct Requirements of CHD4 during B Cell Development and Antibody Response
    Article Snippet: Cross-linked cells were washed with PBS-based Cell Wash Buffer (PBS, 0.1% FBS, 2mM EDTA) and lysed according to the protocol of truChIP Chromatin Shearing Kit with Formaldehyde (Covaris, 520154). .. Cell lysates were sonicated in the M220 Focused-ultrasonicator (Covaris, 5002295) to shear the chromatin, and debris was removed by centrifugation.

    Article Title: Transcription factor activating protein 4 is synthetically lethal and a master regulator of MYCN-amplified neuroblastoma
    Article Snippet: NGP cells were processed following manufacturer’s instruction using truChIP chromatin shearing kit (Covaris #520154). .. NGP cells were processed following manufacturer’s instruction using truChIP chromatin shearing kit (Covaris #520154).

    Binding Assay:

    Article Title: Translational Physiology: Persistent pulmonary hypertension alters the epigenetic characteristics of endothelial nitric oxide synthase gene in pulmonary artery endothelial cells in a fetal lamb model
    Article Snippet: PAEC chromatin was isolated from six different control and PPHN lambs with the tryChIP chromatin shearing reagent kit (#520154, Covaris) and focused-ultrasonicator M220 (Covaris). .. Real-time PCR was used to quantitate the amount of DNA from eNOS promoter regions of two Sp1 binding sites and three proximal ERE half-sites; 5% of input was used as a loading control ( , ).

    Chromatin Immunoprecipitation:

    Article Title: IFI16, a nuclear innate immune DNA sensor, mediates epigenetic silencing of herpesvirus genomes by its association with H3K9 methyltransferases SUV39H1 and GLP
    Article Snippet: .. Chromatin immunoprecipitation Chromatin shearing for ChIP was performed by using the truChIP Chromatin Shearing kit (Covaris #520154) following manufacturer's instructions on a Covaris ME220 focused ultrasonicator. ..

    Article Title: EMT is associated with an epigenetic signature of ECM remodeling genes
    Article Snippet: .. Chromatin immunoprecipitation Chromatin was prepared using the truChIP™ Chromatin Shearing Kit (520154, Covaris, France) according to the manufacturer’s instructions. .. Each sample was submitted to a 8 min sonicated using the M220 Covaris sonicator.

    Article Title: Transcription factor activating protein 4 is synthetically lethal and a master regulator of MYCN-amplified neuroblastoma
    Article Snippet: .. Chromatin immunoprecipitation NGP cells were processed following manufacturer’s instruction using truChIP chromatin shearing kit (Covaris #520154). .. Chromatin was sheared by sonication (Covaris, S-220 series).

    Article Title: TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases
    Article Snippet: Paragraph title: Chromatin immunoprecipitation ... Collected cells were fixed with freshly prepared 1% methanol-free formaldehyde at room temperature for 5 min. Chromatin shearing was performed using truChIP Chromatin Shearing Kit with Formaldehyde (Covaris) on E220 (Covaris) according to the manufacturer’s manual.

    Article Title: In Vitro Cytokine Licensing Induces Persistent Permissive Chromatin at the IDO1 Promoter
    Article Snippet: Paragraph title: Chromatin IP ... Chromatin was cross-linked using truChIP Chromatin Shearing Kit (520154, Covaris, Woburn, MA) according to manufacturer’s instructions, washed twice with cold PBS, and snap-frozen.

    Article Title: Distinct Requirements of CHD4 during B Cell Development and Antibody Response
    Article Snippet: Paragraph title: Chromatin immunoprecipitation ... Cross-linked cells were washed with PBS-based Cell Wash Buffer (PBS, 0.1% FBS, 2mM EDTA) and lysed according to the protocol of truChIP Chromatin Shearing Kit with Formaldehyde (Covaris, 520154).

    Article Title: Transcription factor activating protein 4 is synthetically lethal and a master regulator of MYCN-amplified neuroblastoma
    Article Snippet: Paragraph title: Chromatin immunoprecipitation ... NGP cells were processed following manufacturer’s instruction using truChIP chromatin shearing kit (Covaris #520154).

    Article Title: Translational Physiology: Persistent pulmonary hypertension alters the epigenetic characteristics of endothelial nitric oxide synthase gene in pulmonary artery endothelial cells in a fetal lamb model
    Article Snippet: Paragraph title: Chromatin isolation and chromatin immunoprecipitation assay. ... PAEC chromatin was isolated from six different control and PPHN lambs with the tryChIP chromatin shearing reagent kit (#520154, Covaris) and focused-ultrasonicator M220 (Covaris).

    ChIP-sequencing:

    Article Title: EMT is associated with an epigenetic signature of ECM remodeling genes
    Article Snippet: Chromatin immunoprecipitation Chromatin was prepared using the truChIP™ Chromatin Shearing Kit (520154, Covaris, France) according to the manufacturer’s instructions. .. ChIP were performed using the IP-Star Compact Automated System (Diagenode, Belgium) with the Auto iDeal ChIP-seq Kit for Histones (C01010171, Diagenode) and 1 µg of ChIP-grade antibody or IgG (IG07–2, P.A.R.I.S.).

    Article Title: PDX1 dynamically regulates pancreatic ductal adenocarcinoma initiation and maintenance
    Article Snippet: Paragraph title: ChIP-seq ... Chromatin was prepared from isolated acinar cells or cell lines using the truChIP chromatin-shearing reagent kit (Covaris, 520154).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Covaris truchip chromatin shearing kit with formaldehyde
    Truchip Chromatin Shearing Kit With Formaldehyde, supplied by Covaris, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truchip chromatin shearing kit with formaldehyde/product/Covaris
    Average 94 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    truchip chromatin shearing kit with formaldehyde - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    N/A
    Package of 5 forceps for use with the FFPE tissuePICK
      Buy from Supplier

    N/A
    The Covaris microTUBE 50 is engineered to optimize DNA Shearing in a 50 µl sample volume with Focused ultrasonicators utilizing Adaptive Focused Acoustics AFA technology
      Buy from Supplier

    Image Search Results