truchip low cell chromatin kit  (Covaris)


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    Name:
    truChIP Ultra Low Chromatin Shearing Kit with Formaldehyde
    Description:
    The truChIP Ultra Low Cell Chromatin Shearing Kit with Formaldehyde is designed for efficient chromatin shearing of 100 000 cultured mammalian cells suspension or adherent or less using the Covaris Adaptive Focused Acoustics AFA technology
    Catalog Number:
    520156
    Price:
    None
    Category:
    Reagent
    Applications:
    Chromatin shearing
    Quantity:
    1 0
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    Structured Review

    Covaris truchip low cell chromatin kit
    truChIP Ultra Low Chromatin Shearing Kit with Formaldehyde
    The truChIP Ultra Low Cell Chromatin Shearing Kit with Formaldehyde is designed for efficient chromatin shearing of 100 000 cultured mammalian cells suspension or adherent or less using the Covaris Adaptive Focused Acoustics AFA technology
    https://www.bioz.com/result/truchip low cell chromatin kit/product/Covaris
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    truchip low cell chromatin kit - by Bioz Stars, 2021-05
    91/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program
    Article Snippet: .. Chromatin Immunoprecipitiation Human LECs were harvested and processed for ChIP using a truChIP™ Low Cell Chromatin Shearing Kit with SDS Shearing Buffer (Covaris). .. Sheared chromatin from 10 million cell equivalents was immunoprecipitated with 15 μg rabbit anti-GATA2 antibody (H-116 × , cat. sc-9008; Santa Cruz Biotechnology).

    Article Title: Atopic asthma after rhinovirus‐induced wheezing is associated with DNA methylation change in the SMAD3 gene promoter, et al. Atopic asthma after rhinovirus‐induced wheezing is associated with DNA methylation change in the SMAD3 gene promoter
    Article Snippet: The reduced representation bisulfite sequencing (RRBS) libraries were prepared with a protocol adapted from Boyle et al. , For genomic localization of promoters and enhancers H3K4me1, H3K4me3 and H3K27Ac ChIP‐seq was carried out from peripheral blood mononuclear cells isolated from 2 reference individuals. .. Briefly, the chromatin was prepared (truChIP Low Cell Chromatin Kit, Covaris) and ChIP reactions (Auto Histone ChIP‐seq kit) were carried out with Diagenode IP‐Star SX 8G robot. .. The libraries were sequenced with Illumina HiSeq2000 platform.

    Article Title: Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program
    Article Snippet: .. Human LECs were harvested and processed for ChIP using a truChIP™ Low Cell Chromatin Shearing Kit with SDS Shearing Buffer (Covaris). .. Sheared chromatin from 10 million cell equivalents was immunoprecipitated with 15 μg rabbit anti-GATA2 antibody (H-116 × , cat. sc-9008; Santa Cruz Biotechnology).

    Article Title: GATA4 represses RANKL in osteoblasts via multiple long-range enhancers to regulate osteoclast differentiation
    Article Snippet: For chromatin immunoprecipitation, the calvarial cells were plated at a seeding density of 2 × 105 cells and left undisturbed for 24 h prior to silencing using lentivirus directed towards shC or shGATA4 as described above. .. 24 h after silencing, the cells were washed with PBS and complete MEM media was added to each well and left for additional three days, after which ChIP was performed using truChIP™ Ultra Low Cell Chromatin Shearing Kit (Covaris, Inc., Woburn, Massachusetts). .. The histone 3 lysine 4 dimethylation (H3K4me2, catalog #07–030) and histone 3 lysine 27 trimethylation (H3k27me3, catalog #17–622) antibodies were purchased from Millipore (Burlington, Massachusetts).

    ChIP-sequencing:

    Article Title: Atopic asthma after rhinovirus‐induced wheezing is associated with DNA methylation change in the SMAD3 gene promoter, et al. Atopic asthma after rhinovirus‐induced wheezing is associated with DNA methylation change in the SMAD3 gene promoter
    Article Snippet: The reduced representation bisulfite sequencing (RRBS) libraries were prepared with a protocol adapted from Boyle et al. , For genomic localization of promoters and enhancers H3K4me1, H3K4me3 and H3K27Ac ChIP‐seq was carried out from peripheral blood mononuclear cells isolated from 2 reference individuals. .. Briefly, the chromatin was prepared (truChIP Low Cell Chromatin Kit, Covaris) and ChIP reactions (Auto Histone ChIP‐seq kit) were carried out with Diagenode IP‐Star SX 8G robot. .. The libraries were sequenced with Illumina HiSeq2000 platform.

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  • 91
    Covaris truchip low cell chromatin kit
    Truchip Low Cell Chromatin Kit, supplied by Covaris, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truchip low cell chromatin kit/product/Covaris
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    truchip low cell chromatin kit - by Bioz Stars, 2021-05
    91/100 stars
      Buy from Supplier

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