microtube 15 afa beads strip v2  (Covaris)


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    Name:
    8 microTUBE 15 AFA Beads Strip V2
    Description:
    microTUBE 15 incorporates newly developed AFA BeadsTM Due to their unique surface properties AFA Beads enable fully controllable and repeatable DNA shearing in very low sample volume and at low acoustic power settings 2D barcodes on each microTUBE 15 provide individual sample tracking
    Catalog Number:
    520145
    Product Aliases:
    microTUBE-15
    Price:
    None
    Category:
    Consumable
    Quantity:
    12
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    Structured Review

    Covaris microtube 15 afa beads strip v2
    8 microTUBE 15 AFA Beads Strip V2
    microTUBE 15 incorporates newly developed AFA BeadsTM Due to their unique surface properties AFA Beads enable fully controllable and repeatable DNA shearing in very low sample volume and at low acoustic power settings 2D barcodes on each microTUBE 15 provide individual sample tracking
    https://www.bioz.com/result/microtube 15 afa beads strip v2/product/Covaris
    Average 96 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    microtube 15 afa beads strip v2 - by Bioz Stars, 2019-09
    96/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1
    Article Snippet: Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris). .. Chromatin was sheared by sonication (S220; Covaris) at intensity 4, duty factor 10%, and 200 cycles per burst for 100 s at 4°C.

    Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing
    Article Snippet: DNA extraction was performed by adding 6 M NaCl to the solution, which was then vigorously shaken for 30 seconds prior to a 7 min centrifugation at 10,000xg. .. We used a total processing time of 80 seconds per sample in AFA microTUBE (AFA™Focused-ultrasonicator conditions for the S-Series; Covaris).

    Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction
    Article Snippet: Supernatant was discarded and the pellet was resuspended in 10mL cold Lysis Buffer 2 (10mM Tris-HCl pH 8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, and protease inhibitors), and rotated at room temperature for 10 minutes, followed by centrifugation (250xg, 5 minutes at room temperature). .. Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris AFA tubes (Covaris) Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris).

    Filtration:

    Article Title: Deeper Insight in Beehives: Metagenomes of Royal Jelly, Pollen, and Honey from Lavender, Chestnut, and Fir Honeydew and Epiphytic and Endophytic Microbiota of Lavender and Rose Flowers
    Article Snippet: DNA of epiphytic microbiota from lavender and rose flowers were extracted by incubating flowers in PBS (1×) 0.15% Tween 20 with shaking (400 rpm) for 10 min, followed by a 5-min sonication step, shaking for 10 min (400 rpm) prior to prefiltration, and filtration on a 0.22-µm sterile filter (Millipore, Germany). .. For each sample, 0.1 to 1 µg of DNA was sheared in an AFA microTUBE (Covaris, USA) in an S2 ultrasonicator (Covaris) to achieve an average fragment size of 350 bp.

    Cell Isolation:

    Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis
    Article Snippet: B cells were isolated by negative selection using the human B cell isolation kit II (Miltenyi). .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an AFA microTUBE (Covaris) and a M220 focused ultrasonicator (Covaris).

    Blocking Assay:

    Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction
    Article Snippet: Six hours before lysing cells, 30uL protein G magnetic beads (per ChIP sample) were washed 3 times in blocking buffer (0.5% BSA in PBS); beads were resuspended in 250uL blocking buffer and 2ug antibody (Lamin B: #sc-6217; H3K9me2: #ab1220) and rotated at 4C for at least 6 hours. .. Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris AFA tubes (Covaris) Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris).

    Real-time Polymerase Chain Reaction:

    Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia
    Article Snippet: Fragmentation was done using 8 microTUBE-15 AFA Beads Strip V2 (PN 520159) on Covaris E-series at setting 20%DF, 18W, 200 burst, 20C tempt, 120s treatment time and no Intensifier. .. Only 10-30 ng in 10 μl of fragmented cDNA was used starting from the End Repair step of the Ovation® Ultralow Library Systems V2 (part No. 0344NB) protocol, with 10 cycles PCR.

    Article Title: Genome Sequences of 34 Shiga Toxin-Producing Escherichia coli Isolates from Swine and Other Sources
    Article Snippet: The DNeasy blood and tissue kit (Qiagen, Valencia, CA) and the QIAcube instrument (QIAgen) were used on pure cultures to extract genomic DNA, which was then fragmented using microTUBE-15 AFA beads screw-cap (Covaris, Woburn, MA) and an M220 Focused-ultrasonicator (Covaris) with default settings to achieve a 350-bp peak. .. The DNeasy blood and tissue kit (Qiagen, Valencia, CA) and the QIAcube instrument (QIAgen) were used on pure cultures to extract genomic DNA, which was then fragmented using microTUBE-15 AFA beads screw-cap (Covaris, Woburn, MA) and an M220 Focused-ultrasonicator (Covaris) with default settings to achieve a 350-bp peak.

    Incubation:

    Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1
    Article Snippet: Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris). .. Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris).

    Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis
    Article Snippet: Magnetic beads were blocked (PBS/0.5% BSA) and incubated with either 10 μg SP1 antibody (07-645, Millipore) or control IgG overnight at 4°C. .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an AFA microTUBE (Covaris) and a M220 focused ultrasonicator (Covaris).

    Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction
    Article Snippet: Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris AFA tubes (Covaris) Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris). .. Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris AFA tubes (Covaris) Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris).

    Stripping Membranes:

    Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia
    Article Snippet: The resulting SPIA-cDNA were normalized to 100 ng in 15 μl based on Qubit DNA HS assay. .. Fragmentation was done using 8 microTUBE-15 AFA Beads Strip V2 (PN 520159) on Covaris E-series at setting 20%DF, 18W, 200 burst, 20C tempt, 120s treatment time and no Intensifier. .. Only 10-30 ng in 10 μl of fragmented cDNA was used starting from the End Repair step of the Ovation® Ultralow Library Systems V2 (part No. 0344NB) protocol, with 10 cycles PCR.

    Bradford Assay:

    Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1
    Article Snippet: Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris). .. Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris).

    Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction
    Article Snippet: Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris AFA tubes (Covaris) Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris). .. Lysates were transferred to tubes and Triton X-100 was added (final 1%) followed by centrifugation (top speed, 10 minutes at 4°C in microcentrifuge).

    DNA HS Assay:

    Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia
    Article Snippet: The resulting SPIA-cDNA were normalized to 100 ng in 15 μl based on Qubit DNA HS assay. .. Fragmentation was done using 8 microTUBE-15 AFA Beads Strip V2 (PN 520159) on Covaris E-series at setting 20%DF, 18W, 200 burst, 20C tempt, 120s treatment time and no Intensifier.

    Protein Concentration:

    Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1
    Article Snippet: Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris). .. Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris).

    Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction
    Article Snippet: Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris AFA tubes (Covaris) Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris). .. Lysates were transferred to tubes and Triton X-100 was added (final 1%) followed by centrifugation (top speed, 10 minutes at 4°C in microcentrifuge).

    Sequencing:

    Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia
    Article Snippet: Fragmentation was done using 8 microTUBE-15 AFA Beads Strip V2 (PN 520159) on Covaris E-series at setting 20%DF, 18W, 200 burst, 20C tempt, 120s treatment time and no Intensifier. .. Only 10-30 ng in 10 μl of fragmented cDNA was used starting from the End Repair step of the Ovation® Ultralow Library Systems V2 (part No. 0344NB) protocol, with 10 cycles PCR.

    Article Title: Population Genomics of the Fission Yeast Schizosaccharomyces pombe
    Article Snippet: Paragraph title: Sequencing, mapping, and genotype calling ... Genomic DNA shearing was performed by the AFA system M220 and AFA microTUBE (Covaris) at peak power 140, duty factor 10, cycle/burst 200 at 4°C for 45 seconds.

    Article Title: Deeper Insight in Beehives: Metagenomes of Royal Jelly, Pollen, and Honey from Lavender, Chestnut, and Fir Honeydew and Epiphytic and Endophytic Microbiota of Lavender and Rose Flowers
    Article Snippet: For each sample, 0.1 to 1 µg of DNA was sheared in an AFA microTUBE (Covaris, USA) in an S2 ultrasonicator (Covaris) to achieve an average fragment size of 350 bp. .. For each sample, 0.1 to 1 µg of DNA was sheared in an AFA microTUBE (Covaris, USA) in an S2 ultrasonicator (Covaris) to achieve an average fragment size of 350 bp.

    Article Title: Genome Sequences of 34 Shiga Toxin-Producing Escherichia coli Isolates from Swine and Other Sources
    Article Snippet: To increase the inventory of swine STEC genome sequences and better characterize the STEC population in pigs, whole-genome sequencing was performed on 29 strains isolated from asymptomatic swine and 3 beef trim isolates belonging to uncommon or difficult-to-serotype O groups, and 2 strains isolated from swine with postweaning diarrhea or edema disease ( ). .. The DNeasy blood and tissue kit (Qiagen, Valencia, CA) and the QIAcube instrument (QIAgen) were used on pure cultures to extract genomic DNA, which was then fragmented using microTUBE-15 AFA beads screw-cap (Covaris, Woburn, MA) and an M220 Focused-ultrasonicator (Covaris) with default settings to achieve a 350-bp peak.

    Article Title: Metagenome-Assembled Genome Sequence of Rhodopseudomonas palustris Strain ELI 1980, Commercialized as a Biostimulant
    Article Snippet: Metagenomic DNA was sheared in an AFA microtube (Covaris, USA) in an S2 ultrasonicator (Covaris) to achieve an average fragment size of 350 bp. .. Metagenomic DNA was sheared in an AFA microtube (Covaris, USA) in an S2 ultrasonicator (Covaris) to achieve an average fragment size of 350 bp.

    Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing
    Article Snippet: After quantification (Nanodrop), 5 μg of DNA for each indexed library (n = 6) was used to prepare the NGS whole-genome sequencing assay following the manufacturer’s instructions (TruSeqTM Sample Prep Kit-v2, Illumina). .. We used a total processing time of 80 seconds per sample in AFA microTUBE (AFA™Focused-ultrasonicator conditions for the S-Series; Covaris).

    Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction
    Article Snippet: Paragraph title: ChIP and library preparation for sequencing ... Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris AFA tubes (Covaris) Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris).

    Sonication:

    Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1
    Article Snippet: Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris). .. Chromatin was sheared by sonication (S220; Covaris) at intensity 4, duty factor 10%, and 200 cycles per burst for 100 s at 4°C.

    Article Title: Deeper Insight in Beehives: Metagenomes of Royal Jelly, Pollen, and Honey from Lavender, Chestnut, and Fir Honeydew and Epiphytic and Endophytic Microbiota of Lavender and Rose Flowers
    Article Snippet: DNA of epiphytic microbiota from lavender and rose flowers were extracted by incubating flowers in PBS (1×) 0.15% Tween 20 with shaking (400 rpm) for 10 min, followed by a 5-min sonication step, shaking for 10 min (400 rpm) prior to prefiltration, and filtration on a 0.22-µm sterile filter (Millipore, Germany). .. For each sample, 0.1 to 1 µg of DNA was sheared in an AFA microTUBE (Covaris, USA) in an S2 ultrasonicator (Covaris) to achieve an average fragment size of 350 bp.

    Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction
    Article Snippet: Supernatant was discarded and the pellet was resuspended in 10mL cold Lysis Buffer 2 (10mM Tris-HCl pH 8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, and protease inhibitors), and rotated at room temperature for 10 minutes, followed by centrifugation (250xg, 5 minutes at room temperature). .. Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris AFA tubes (Covaris) Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris). .. Lysates were transferred to tubes and Triton X-100 was added (final 1%) followed by centrifugation (top speed, 10 minutes at 4°C in microcentrifuge).

    Hi-C:

    Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing
    Article Snippet: We used a total processing time of 80 seconds per sample in AFA microTUBE (AFA™Focused-ultrasonicator conditions for the S-Series; Covaris). .. We used a total processing time of 80 seconds per sample in AFA microTUBE (AFA™Focused-ultrasonicator conditions for the S-Series; Covaris).

    DNA Extraction:

    Article Title: Metagenomes of Soils Samples from an Established Perennial Cropping System of Asparagus Treated with Biostimulants in Southern France
    Article Snippet: Metagenomic DNA samples were extracted using the PowerSoil DNA isolation kit (Mo Bio, Inc./Qiagen, USA). .. One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an AFA microtube (Covaris, USA) using an S2 ultrasonicator (Covaris).

    Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing
    Article Snippet: DNA extraction was performed by adding 6 M NaCl to the solution, which was then vigorously shaken for 30 seconds prior to a 7 min centrifugation at 10,000xg. .. We used a total processing time of 80 seconds per sample in AFA microTUBE (AFA™Focused-ultrasonicator conditions for the S-Series; Covaris).

    Nucleic Acid Electrophoresis:

    Article Title: Metagenomes of Soils Samples from an Established Perennial Cropping System of Asparagus Treated with Biostimulants in Southern France
    Article Snippet: One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an AFA microtube (Covaris, USA) using an S2 ultrasonicator (Covaris). .. One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an AFA microtube (Covaris, USA) using an S2 ultrasonicator (Covaris).

    RNA Sequencing Assay:

    Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia
    Article Snippet: Paragraph title: Sample Preparation for RNA-Sequencing ... Fragmentation was done using 8 microTUBE-15 AFA Beads Strip V2 (PN 520159) on Covaris E-series at setting 20%DF, 18W, 200 burst, 20C tempt, 120s treatment time and no Intensifier.

    Magnetic Beads:

    Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis
    Article Snippet: Magnetic beads were blocked (PBS/0.5% BSA) and incubated with either 10 μg SP1 antibody (07-645, Millipore) or control IgG overnight at 4°C. .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an AFA microTUBE (Covaris) and a M220 focused ultrasonicator (Covaris).

    Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction
    Article Snippet: Six hours before lysing cells, 30uL protein G magnetic beads (per ChIP sample) were washed 3 times in blocking buffer (0.5% BSA in PBS); beads were resuspended in 250uL blocking buffer and 2ug antibody (Lamin B: #sc-6217; H3K9me2: #ab1220) and rotated at 4C for at least 6 hours. .. Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris AFA tubes (Covaris) Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris).

    Isolation:

    Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis
    Article Snippet: Magnetic beads were blocked (PBS/0.5% BSA) and incubated with either 10 μg SP1 antibody (07-645, Millipore) or control IgG overnight at 4°C. .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an AFA microTUBE (Covaris) and a M220 focused ultrasonicator (Covaris). .. Conditions for sonication: 20% duty cycle, 30 min. Sonicated samples were immunoprecipitated with magnetically labeled SP1 antibody or control IgG overnight at 4°C.

    Article Title: Genome Sequences of 34 Shiga Toxin-Producing Escherichia coli Isolates from Swine and Other Sources
    Article Snippet: To increase the inventory of swine STEC genome sequences and better characterize the STEC population in pigs, whole-genome sequencing was performed on 29 strains isolated from asymptomatic swine and 3 beef trim isolates belonging to uncommon or difficult-to-serotype O groups, and 2 strains isolated from swine with postweaning diarrhea or edema disease ( ). .. The DNeasy blood and tissue kit (Qiagen, Valencia, CA) and the QIAcube instrument (QIAgen) were used on pure cultures to extract genomic DNA, which was then fragmented using microTUBE-15 AFA beads screw-cap (Covaris, Woburn, MA) and an M220 Focused-ultrasonicator (Covaris) with default settings to achieve a 350-bp peak.

    Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing
    Article Snippet: We used a total processing time of 80 seconds per sample in AFA microTUBE (AFA™Focused-ultrasonicator conditions for the S-Series; Covaris). .. For high-resolution chromosome conformation capture (Hi-C) libraries , DNA was prepared using a previously published protocol .

    Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction
    Article Snippet: Crude nuclei were isolated from frozen crosslinked cells as follows: cell pellet (from 10cm plate) was resuspended in 10mL cold Lysis Buffer 1 (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% Glycerol, 0.5% NP-40, 0.25% Triton X-100, and protease inhibitors), and rotated at 4°C for 10 minutes, followed by centrifugation (250xg, 5 minutes at room temperature). .. Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris AFA tubes (Covaris) Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris).

    Microscopy:

    Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1
    Article Snippet: After thawing, cells were resuspended in 250 µl ChIP buffer 1 containing protease inhibitors and broken by glass bead lysis during five cycles (60 s at 6.5 m/s, 3-min cooling on ice between each cycle; MP FastPrep). .. Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris). .. Chromatin was sheared by sonication (S220; Covaris) at intensity 4, duty factor 10%, and 200 cycles per burst for 100 s at 4°C.

    Purification:

    Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis
    Article Snippet: Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an AFA microTUBE (Covaris) and a M220 focused ultrasonicator (Covaris). .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an AFA microTUBE (Covaris) and a M220 focused ultrasonicator (Covaris).

    Polymerase Chain Reaction:

    Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis
    Article Snippet: Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an AFA microTUBE (Covaris) and a M220 focused ultrasonicator (Covaris). .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an AFA microTUBE (Covaris) and a M220 focused ultrasonicator (Covaris).

    Shotgun Sequencing:

    Article Title: Deeper Insight in Beehives: Metagenomes of Royal Jelly, Pollen, and Honey from Lavender, Chestnut, and Fir Honeydew and Epiphytic and Endophytic Microbiota of Lavender and Rose Flowers
    Article Snippet: For each sample, 0.1 to 1 µg of DNA was sheared in an AFA microTUBE (Covaris, USA) in an S2 ultrasonicator (Covaris) to achieve an average fragment size of 350 bp. .. Libraries were created using the TruSeq DNA PCR-free and TruSeq Nano DNA library preparation kits (Illumina, USA), and the insert size was checked in a Fragment analyzer (Advanced Analytical Technologies, Inc., USA).

    Article Title: Metagenomes of Soils Samples from an Established Perennial Cropping System of Asparagus Treated with Biostimulants in Southern France
    Article Snippet: One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an AFA microtube (Covaris, USA) using an S2 ultrasonicator (Covaris). .. Libraries were produced with the TruSeq DNA PCR-free library kit (Illumina).

    Article Title: Metagenome-Assembled Genome Sequence of Rhodopseudomonas palustris Strain ELI 1980, Commercialized as a Biostimulant
    Article Snippet: Metagenomic DNA was sheared in an AFA microtube (Covaris, USA) in an S2 ultrasonicator (Covaris) to achieve an average fragment size of 350 bp. .. The sequencing library was created with the TruSeq DNA PCR-free library kit (Illumina, USA).

    Chromatin Immunoprecipitation:

    Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1
    Article Snippet: Paragraph title: ChIP ... Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris).

    Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis
    Article Snippet: Paragraph title: Chromatin immunoprecipitation ... Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an AFA microTUBE (Covaris) and a M220 focused ultrasonicator (Covaris).

    Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction
    Article Snippet: Paragraph title: ChIP and library preparation for sequencing ... Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris AFA tubes (Covaris) Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris).

    Selection:

    Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis
    Article Snippet: B cells were isolated by negative selection using the human B cell isolation kit II (Miltenyi). .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an AFA microTUBE (Covaris) and a M220 focused ultrasonicator (Covaris).

    Sample Prep:

    Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis
    Article Snippet: The cell pellet was frozen at −80°C until sample preparation. .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an AFA microTUBE (Covaris) and a M220 focused ultrasonicator (Covaris).

    Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia
    Article Snippet: Paragraph title: Sample Preparation for RNA-Sequencing ... Fragmentation was done using 8 microTUBE-15 AFA Beads Strip V2 (PN 520159) on Covaris E-series at setting 20%DF, 18W, 200 burst, 20C tempt, 120s treatment time and no Intensifier.

    Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing
    Article Snippet: After quantification (Nanodrop), 5 μg of DNA for each indexed library (n = 6) was used to prepare the NGS whole-genome sequencing assay following the manufacturer’s instructions (TruSeqTM Sample Prep Kit-v2, Illumina). .. We used a total processing time of 80 seconds per sample in AFA microTUBE (AFA™Focused-ultrasonicator conditions for the S-Series; Covaris).

    Homogenization:

    Article Title: Metagenomes of Soils Samples from an Established Perennial Cropping System of Asparagus Treated with Biostimulants in Southern France
    Article Snippet: After thawing and homogenization, subsamples (10 g) were disrupted with TissueLyser II (Qiagen, Germany). .. One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an AFA microtube (Covaris, USA) using an S2 ultrasonicator (Covaris).

    Next-Generation Sequencing:

    Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing
    Article Snippet: Paragraph title: NGS library preparation ... We used a total processing time of 80 seconds per sample in AFA microTUBE (AFA™Focused-ultrasonicator conditions for the S-Series; Covaris).

    Spectrophotometry:

    Article Title: Metagenomes of Soils Samples from an Established Perennial Cropping System of Asparagus Treated with Biostimulants in Southern France
    Article Snippet: One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an AFA microtube (Covaris, USA) using an S2 ultrasonicator (Covaris). .. One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an AFA microtube (Covaris, USA) using an S2 ultrasonicator (Covaris).

    Produced:

    Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing
    Article Snippet: We used a total processing time of 80 seconds per sample in AFA microTUBE (AFA™Focused-ultrasonicator conditions for the S-Series; Covaris). .. For high-resolution chromosome conformation capture (Hi-C) libraries , DNA was prepared using a previously published protocol .

    Concentration Assay:

    Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1
    Article Snippet: Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris). .. Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris).

    Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia
    Article Snippet: Most of the total RNA has average RIN number 5-8, with a concentration at least 4 pg/μl. .. Fragmentation was done using 8 microTUBE-15 AFA Beads Strip V2 (PN 520159) on Covaris E-series at setting 20%DF, 18W, 200 burst, 20C tempt, 120s treatment time and no Intensifier.

    Article Title: Genome Sequences of 34 Shiga Toxin-Producing Escherichia coli Isolates from Swine and Other Sources
    Article Snippet: The DNeasy blood and tissue kit (Qiagen, Valencia, CA) and the QIAcube instrument (QIAgen) were used on pure cultures to extract genomic DNA, which was then fragmented using microTUBE-15 AFA beads screw-cap (Covaris, Woburn, MA) and an M220 Focused-ultrasonicator (Covaris) with default settings to achieve a 350-bp peak. .. The DNeasy blood and tissue kit (Qiagen, Valencia, CA) and the QIAcube instrument (QIAgen) were used on pure cultures to extract genomic DNA, which was then fragmented using microTUBE-15 AFA beads screw-cap (Covaris, Woburn, MA) and an M220 Focused-ultrasonicator (Covaris) with default settings to achieve a 350-bp peak.

    Lysis:

    Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1
    Article Snippet: After thawing, cells were resuspended in 250 µl ChIP buffer 1 containing protease inhibitors and broken by glass bead lysis during five cycles (60 s at 6.5 m/s, 3-min cooling on ice between each cycle; MP FastPrep). .. Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris). .. Chromatin was sheared by sonication (S220; Covaris) at intensity 4, duty factor 10%, and 200 cycles per burst for 100 s at 4°C.

    Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction
    Article Snippet: Supernatant was discarded and the pellet was resuspended in 10mL cold Lysis Buffer 2 (10mM Tris-HCl pH 8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, and protease inhibitors), and rotated at room temperature for 10 minutes, followed by centrifugation (250xg, 5 minutes at room temperature). .. Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris AFA tubes (Covaris) Samples were sonicated using a Covaris S220 sonicator (high cell chromatin shearing for 15 minutes; Covaris). .. Lysates were transferred to tubes and Triton X-100 was added (final 1%) followed by centrifugation (top speed, 10 minutes at 4°C in microcentrifuge).

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  • 99
    Covaris truxtrac ffpe dna kit
    Variants identified in the samples extracted with different <t>DNA</t> extraction methods. (A-D) Venn diagram showing the distribution of variants between DNA extraction methods in four different <t>FFPE</t> samples, SARC1-4. Variants were detected by MuTect and Strelka, using an artificial control as normal. The data was filtered on exonic variants with allele frequency > 5% and coverage > 100x, being present at
    Truxtrac Ffpe Dna Kit, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truxtrac ffpe dna kit/product/Covaris
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    truxtrac ffpe dna kit - by Bioz Stars, 2019-09
    99/100 stars
      Buy from Supplier

    99
    Covaris dna shearing settings
    Overview of data generation and collection. <t>*Illumina</t> acquired extracted <t>DNA</t> from ATCC for library construction, sequencing, and analysis.
    Dna Shearing Settings, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna shearing settings/product/Covaris
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna shearing settings - by Bioz Stars, 2019-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Variants identified in the samples extracted with different DNA extraction methods. (A-D) Venn diagram showing the distribution of variants between DNA extraction methods in four different FFPE samples, SARC1-4. Variants were detected by MuTect and Strelka, using an artificial control as normal. The data was filtered on exonic variants with allele frequency > 5% and coverage > 100x, being present at

    Journal: PLoS ONE

    Article Title: Evaluation of commercial DNA and RNA extraction methods for high-throughput sequencing of FFPE samples

    doi: 10.1371/journal.pone.0197456

    Figure Lengend Snippet: Variants identified in the samples extracted with different DNA extraction methods. (A-D) Venn diagram showing the distribution of variants between DNA extraction methods in four different FFPE samples, SARC1-4. Variants were detected by MuTect and Strelka, using an artificial control as normal. The data was filtered on exonic variants with allele frequency > 5% and coverage > 100x, being present at

    Article Snippet: For DNA extraction, the truXTRAC FFPE DNA kit from Covaris gave in general higher yield and more intact DNA fragments, however, no significant differences were observed in the quantity of libraries, DNA sequencing or variant calling among the different extraction methods tested.

    Techniques: DNA Extraction, Formalin-fixed Paraffin-Embedded

    Yield and amplifiability of extracted DNA and RNA. (A) Average total amount of DNA. (B) Average total amount of RNA. (C) Amplifiable DNA quantified with the FFPE QC kit from Illumina. (D) Amplifiable RNA quantified with the PreSeq QC assay from ArcherDx. The average total amount and average delta Ct values for the different samples and extraction methods are shown. The standard deviation is shown as vertical bars. Methods with significant differences in yield are marked as connected with horizontal bars (p

    Journal: PLoS ONE

    Article Title: Evaluation of commercial DNA and RNA extraction methods for high-throughput sequencing of FFPE samples

    doi: 10.1371/journal.pone.0197456

    Figure Lengend Snippet: Yield and amplifiability of extracted DNA and RNA. (A) Average total amount of DNA. (B) Average total amount of RNA. (C) Amplifiable DNA quantified with the FFPE QC kit from Illumina. (D) Amplifiable RNA quantified with the PreSeq QC assay from ArcherDx. The average total amount and average delta Ct values for the different samples and extraction methods are shown. The standard deviation is shown as vertical bars. Methods with significant differences in yield are marked as connected with horizontal bars (p

    Article Snippet: For DNA extraction, the truXTRAC FFPE DNA kit from Covaris gave in general higher yield and more intact DNA fragments, however, no significant differences were observed in the quantity of libraries, DNA sequencing or variant calling among the different extraction methods tested.

    Techniques: Formalin-fixed Paraffin-Embedded, Standard Deviation

    Overview of data generation and collection. *Illumina acquired extracted DNA from ATCC for library construction, sequencing, and analysis.

    Journal: Scientific Reports

    Article Title: A somatic reference standard for cancer genome sequencing

    doi: 10.1038/srep24607

    Figure Lengend Snippet: Overview of data generation and collection. *Illumina acquired extracted DNA from ATCC for library construction, sequencing, and analysis.

    Article Snippet: 500 ng of each DNA was prepared using the TruSeq DNA PCR-Free Sample Preparation Kit (Illumina® , cat# FC-121-3001) following the manufacturer’s protocol with modified Covaris DNA shearing settings: Duty cycle 10%, Cycles/Burst 200, Time 45 sec.

    Techniques: Sequencing