microtube 15 afa beads strip v2 (Covaris)
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Name:
8 microTUBE 15 AFA Beads Strip V2
Description:
microTUBE 15 incorporates newly developed AFA BeadsTM Due to their unique surface properties AFA Beads enable fully controllable and repeatable DNA shearing in very low sample volume and at low acoustic power settings 2D barcodes on each microTUBE 15 provide individual sample tracking
Catalog Number:
520145
Product Aliases:
microTUBE-15
Price:
None
Category:
Consumable
Score:
85
Quantity:
12
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Structured Review
Covaris
microtube 15 afa beads strip v2
microTUBE 15 incorporates newly developed AFA BeadsTM Due to their unique surface properties AFA Beads enable fully controllable and repeatable DNA shearing in very low sample volume and at low acoustic power settings 2D barcodes on each microTUBE 15 provide individual sample tracking
https://www.bioz.com/result/microtube 15 afa beads strip v2/product/Covaris
Average 96 stars, based on 11 article reviews
Price from $9.99 to $1999.99
microTUBE 15 incorporates newly developed AFA BeadsTM Due to their unique surface properties AFA Beads enable fully controllable and repeatable DNA shearing in very low sample volume and at low acoustic power settings 2D barcodes on each microTUBE 15 provide individual sample tracking
https://www.bioz.com/result/microtube 15 afa beads strip v2/product/Covaris
Average 96 stars, based on 11 article reviews
Price from $9.99 to $1999.99
microtube 15 afa beads strip v2 - by Bioz Stars,
2019-11
96/100 stars
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Centrifugation:Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1 Article Snippet: Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing Article Snippet: DNA extraction was performed by adding 6 M NaCl to the solution, which was then vigorously shaken for 30 seconds prior to a 7 min centrifugation at 10,000xg. .. We used a total processing time of 80 seconds per sample in Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction Article Snippet: Supernatant was discarded and the pellet was resuspended in 10mL cold Lysis Buffer 2 (10mM Tris-HCl pH 8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, and protease inhibitors), and rotated at room temperature for 10 minutes, followed by centrifugation (250xg, 5 minutes at room temperature). .. Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris Filtration:Article Title: Deeper Insight in Beehives: Metagenomes of Royal Jelly, Pollen, and Honey from Lavender, Chestnut, and Fir Honeydew and Epiphytic and Endophytic Microbiota of Lavender and Rose Flowers Article Snippet: DNA of epiphytic microbiota from lavender and rose flowers were extracted by incubating flowers in PBS (1×) 0.15% Tween 20 with shaking (400 rpm) for 10 min, followed by a 5-min sonication step, shaking for 10 min (400 rpm) prior to prefiltration, and filtration on a 0.22-µm sterile filter (Millipore, Germany). .. For each sample, 0.1 to 1 µg of DNA was sheared in an Cell Isolation:Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis Article Snippet: B cells were isolated by negative selection using the human B cell isolation kit II (Miltenyi). .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an Blocking Assay:Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction Article Snippet: Six hours before lysing cells, 30uL protein G magnetic beads (per ChIP sample) were washed 3 times in blocking buffer (0.5% BSA in PBS); beads were resuspended in 250uL blocking buffer and 2ug antibody (Lamin B: #sc-6217; H3K9me2: #ab1220) and rotated at 4C for at least 6 hours. .. Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris Real-time Polymerase Chain Reaction:Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia Article Snippet: Fragmentation was done using 8 Article Title: Genome Sequences of 34 Shiga Toxin-Producing Escherichia coli Isolates from Swine and Other Sources Article Snippet: The DNeasy blood and tissue kit (Qiagen, Valencia, CA) and the QIAcube instrument (QIAgen) were used on pure cultures to extract genomic DNA, which was then fragmented using Incubation:Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1 Article Snippet: Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a microTUBE AFA (520045; Covaris). .. Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis Article Snippet: Magnetic beads were blocked (PBS/0.5% BSA) and incubated with either 10 μg SP1 antibody (07-645, Millipore) or control IgG overnight at 4°C. .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction Article Snippet: Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris Stripping Membranes:Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia Article Snippet: The resulting SPIA-cDNA were normalized to 100 ng in 15 μl based on Qubit DNA HS assay. .. Fragmentation was done using 8 Bradford Assay:Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1 Article Snippet: Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction Article Snippet: Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris DNA HS Assay:Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia Article Snippet: The resulting SPIA-cDNA were normalized to 100 ng in 15 μl based on Qubit DNA HS assay. .. Fragmentation was done using 8 Protein Concentration:Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1 Article Snippet: Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction Article Snippet: Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris Sequencing:Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia Article Snippet: Fragmentation was done using 8 Article Title: Genome Sequences of 34 Shiga Toxin-Producing Escherichia coli Isolates from Swine and Other Sources Article Snippet: To increase the inventory of swine STEC genome sequences and better characterize the STEC population in pigs, whole-genome sequencing was performed on 29 strains isolated from asymptomatic swine and 3 beef trim isolates belonging to uncommon or difficult-to-serotype O groups, and 2 strains isolated from swine with postweaning diarrhea or edema disease ( ). .. The DNeasy blood and tissue kit (Qiagen, Valencia, CA) and the QIAcube instrument (QIAgen) were used on pure cultures to extract genomic DNA, which was then fragmented using Article Title: Population Genomics of the Fission Yeast Schizosaccharomyces pombe Article Snippet: Paragraph title: Sequencing, mapping, and genotype calling ... Genomic DNA shearing was performed by the AFA system M220 and Article Title: Deeper Insight in Beehives: Metagenomes of Royal Jelly, Pollen, and Honey from Lavender, Chestnut, and Fir Honeydew and Epiphytic and Endophytic Microbiota of Lavender and Rose Flowers Article Snippet: For each sample, 0.1 to 1 µg of DNA was sheared in an Article Title: Metagenome-Assembled Genome Sequence of Rhodopseudomonas palustris Strain ELI 1980, Commercialized as a Biostimulant Article Snippet: Metagenomic DNA was sheared in an AFA microtube (Covaris, USA) in an S2 ultrasonicator (Covaris) to achieve an average fragment size of 350 bp. .. Metagenomic DNA was sheared in an Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing Article Snippet: After quantification (Nanodrop), 5 μg of DNA for each indexed library (n = 6) was used to prepare the NGS whole-genome sequencing assay following the manufacturer’s instructions (TruSeqTM Sample Prep Kit-v2, Illumina). .. We used a total processing time of 80 seconds per sample in Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction Article Snippet: Paragraph title: ChIP and library preparation for sequencing ... Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris Sonication:Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1 Article Snippet: Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a Article Title: Deeper Insight in Beehives: Metagenomes of Royal Jelly, Pollen, and Honey from Lavender, Chestnut, and Fir Honeydew and Epiphytic and Endophytic Microbiota of Lavender and Rose Flowers Article Snippet: DNA of epiphytic microbiota from lavender and rose flowers were extracted by incubating flowers in PBS (1×) 0.15% Tween 20 with shaking (400 rpm) for 10 min, followed by a 5-min sonication step, shaking for 10 min (400 rpm) prior to prefiltration, and filtration on a 0.22-µm sterile filter (Millipore, Germany). .. For each sample, 0.1 to 1 µg of DNA was sheared in an Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction Article Snippet: Supernatant was discarded and the pellet was resuspended in 10mL cold Lysis Buffer 2 (10mM Tris-HCl pH 8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, and protease inhibitors), and rotated at room temperature for 10 minutes, followed by centrifugation (250xg, 5 minutes at room temperature). .. Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris Hi-C:Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing Article Snippet: We used a total processing time of 80 seconds per sample in DNA Extraction:Article Title: Metagenomes of Soils Samples from an Established Perennial Cropping System of Asparagus Treated with Biostimulants in Southern France Article Snippet: Metagenomic DNA samples were extracted using the PowerSoil DNA isolation kit (Mo Bio, Inc./Qiagen, USA). .. One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing Article Snippet: DNA extraction was performed by adding 6 M NaCl to the solution, which was then vigorously shaken for 30 seconds prior to a 7 min centrifugation at 10,000xg. .. We used a total processing time of 80 seconds per sample in Nucleic Acid Electrophoresis:Article Title: Metagenomes of Soils Samples from an Established Perennial Cropping System of Asparagus Treated with Biostimulants in Southern France Article Snippet: One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an AFA microtube (Covaris, USA) using an S2 ultrasonicator (Covaris). .. One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an RNA Sequencing Assay:Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia Article Snippet: Paragraph title: Sample Preparation for RNA-Sequencing ... Fragmentation was done using 8 Magnetic Beads:Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis Article Snippet: Magnetic beads were blocked (PBS/0.5% BSA) and incubated with either 10 μg SP1 antibody (07-645, Millipore) or control IgG overnight at 4°C. .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction Article Snippet: Six hours before lysing cells, 30uL protein G magnetic beads (per ChIP sample) were washed 3 times in blocking buffer (0.5% BSA in PBS); beads were resuspended in 250uL blocking buffer and 2ug antibody (Lamin B: #sc-6217; H3K9me2: #ab1220) and rotated at 4C for at least 6 hours. .. Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris Isolation:Article Title: Genome Sequences of 34 Shiga Toxin-Producing Escherichia coli Isolates from Swine and Other Sources Article Snippet: To increase the inventory of swine STEC genome sequences and better characterize the STEC population in pigs, whole-genome sequencing was performed on 29 strains isolated from asymptomatic swine and 3 beef trim isolates belonging to uncommon or difficult-to-serotype O groups, and 2 strains isolated from swine with postweaning diarrhea or edema disease ( ). .. The DNeasy blood and tissue kit (Qiagen, Valencia, CA) and the QIAcube instrument (QIAgen) were used on pure cultures to extract genomic DNA, which was then fragmented using Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis Article Snippet: Magnetic beads were blocked (PBS/0.5% BSA) and incubated with either 10 μg SP1 antibody (07-645, Millipore) or control IgG overnight at 4°C. .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing Article Snippet: We used a total processing time of 80 seconds per sample in Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction Article Snippet: Crude nuclei were isolated from frozen crosslinked cells as follows: cell pellet (from 10cm plate) was resuspended in 10mL cold Lysis Buffer 1 (50mM HEPES-KOH pH7.5, 140mM NaCl, 1mM EDTA, 10% Glycerol, 0.5% NP-40, 0.25% Triton X-100, and protease inhibitors), and rotated at 4°C for 10 minutes, followed by centrifugation (250xg, 5 minutes at room temperature). .. Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris Microscopy:Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1 Article Snippet: After thawing, cells were resuspended in 250 µl ChIP buffer 1 containing protease inhibitors and broken by glass bead lysis during five cycles (60 s at 6.5 m/s, 3-min cooling on ice between each cycle; MP FastPrep). .. Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a Purification:Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis Article Snippet: Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an Polymerase Chain Reaction:Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis Article Snippet: Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an Shotgun Sequencing:Article Title: Deeper Insight in Beehives: Metagenomes of Royal Jelly, Pollen, and Honey from Lavender, Chestnut, and Fir Honeydew and Epiphytic and Endophytic Microbiota of Lavender and Rose Flowers Article Snippet: For each sample, 0.1 to 1 µg of DNA was sheared in an Article Title: Metagenomes of Soils Samples from an Established Perennial Cropping System of Asparagus Treated with Biostimulants in Southern France Article Snippet: One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an Article Title: Metagenome-Assembled Genome Sequence of Rhodopseudomonas palustris Strain ELI 1980, Commercialized as a Biostimulant Article Snippet: Metagenomic DNA was sheared in an Chromatin Immunoprecipitation:Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1 Article Snippet: Paragraph title: ChIP ... Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis Article Snippet: Paragraph title: Chromatin immunoprecipitation ... Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction Article Snippet: Paragraph title: ChIP and library preparation for sequencing ... Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris Selection:Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis Article Snippet: B cells were isolated by negative selection using the human B cell isolation kit II (Miltenyi). .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an Sample Prep:Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia Article Snippet: Paragraph title: Sample Preparation for RNA-Sequencing ... Fragmentation was done using 8 Article Title: Increased Binding of Specificity Protein 1 to the IL21R Promoter in B Cells Results in Enhanced B Cell Responses in Rheumatoid Arthritis Article Snippet: The cell pellet was frozen at −80°C until sample preparation. .. Nuclei were isolated the following day and sheared using a truChIP Chromatin Shearing Reagent Kit (Covaris), an Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing Article Snippet: After quantification (Nanodrop), 5 μg of DNA for each indexed library (n = 6) was used to prepare the NGS whole-genome sequencing assay following the manufacturer’s instructions (TruSeqTM Sample Prep Kit-v2, Illumina). .. We used a total processing time of 80 seconds per sample in Homogenization:Article Title: Metagenomes of Soils Samples from an Established Perennial Cropping System of Asparagus Treated with Biostimulants in Southern France Article Snippet: After thawing and homogenization, subsamples (10 g) were disrupted with TissueLyser II (Qiagen, Germany). .. One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an Next-Generation Sequencing:Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing Article Snippet: Paragraph title: NGS library preparation ... We used a total processing time of 80 seconds per sample in Spectrophotometry:Article Title: Metagenomes of Soils Samples from an Established Perennial Cropping System of Asparagus Treated with Biostimulants in Southern France Article Snippet: One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an AFA microtube (Covaris, USA) using an S2 ultrasonicator (Covaris). .. One microgram of metagenomic DNA was sheared to an average fragment size of 350 bp in an Produced:Article Title: Comparison of DNA Quantification Methods for Next Generation Sequencing Article Snippet: We used a total processing time of 80 seconds per sample in Concentration Assay:Article Title: Increased Vascular Permeability in the Bone Marrow Microenvironment Contributes to Disease Progression and Drug Response in Acute Myeloid Leukemia Article Snippet: Most of the total RNA has average RIN number 5-8, with a concentration at least 4 pg/μl. .. Fragmentation was done using 8 Article Title: Genome Sequences of 34 Shiga Toxin-Producing Escherichia coli Isolates from Swine and Other Sources Article Snippet: The DNeasy blood and tissue kit (Qiagen, Valencia, CA) and the QIAcube instrument (QIAgen) were used on pure cultures to extract genomic DNA, which was then fragmented using Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1 Article Snippet: Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a Lysis:Article Title: Control of mitotic chromosome condensation by the fission yeast transcription factor Zas1 Article Snippet: After thawing, cells were resuspended in 250 µl ChIP buffer 1 containing protease inhibitors and broken by glass bead lysis during five cycles (60 s at 6.5 m/s, 3-min cooling on ice between each cycle; MP FastPrep). .. Lysis efficiency was confirmed by microscopy, and 130 µl suspension was transferred to a Article Title: Genome-nuclear lamina interactions regulate cardiac stem cell lineage restriction Article Snippet: Supernatant was discarded and the pellet was resuspended in 10mL cold Lysis Buffer 2 (10mM Tris-HCl pH 8.0, 200mM NaCl, 1mM EDTA, 0.5mM EGTA, and protease inhibitors), and rotated at room temperature for 10 minutes, followed by centrifugation (250xg, 5 minutes at room temperature). .. Supernatant was discarded and nuclei were resuspended/lysed in 1mL cold Lysis Buffer 3 (10mM Tris-HCl, pH 8.0, 100mM NaCl, 1mM EDTA, 0.5mM EGTA, 0.1% Na-Deoxycholate, and protease inhibitors) and transferred to pre-chilled 1mL Covaris |
