cryofracture  (Covaris)


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    Name:
    truXTRAC Protein Extraction Buffer SuperB
    Description:
    Enhanced native protein extraction for ELISAs and immunoassays Optimized for native applications preserving protein conformation and biological activity Enhances protein yields while preserving native conformation antigenicity and biological activity Doubles protein yeilds from tissues Extracted proteins are directly compatible with immunoassy protein assay and affinity enrichment
    Catalog Number:
    520112
    Price:
    None
    Applications:
    Protein Extraction
    Category:
    Reagent
    Quantity:
    1 0
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    Structured Review

    Covaris cryofracture
    truXTRAC Protein Extraction Buffer SuperB
    Enhanced native protein extraction for ELISAs and immunoassays Optimized for native applications preserving protein conformation and biological activity Enhances protein yields while preserving native conformation antigenicity and biological activity Doubles protein yeilds from tissues Extracted proteins are directly compatible with immunoassy protein assay and affinity enrichment
    https://www.bioz.com/result/cryofracture/product/Covaris
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    cryofracture - by Bioz Stars, 2020-04
    95/100 stars

    Related Products / Commonly Used Together

    bca protein assay kit
    truxtrac protein extraction
    cryofracture

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    Related Articles

    In Vivo:

    Article Title: Neuropilin-1 drives tumor-specific uptake of chlorotoxin
    Article Snippet: Paragraph title: In vivo studies ... Following cryofracture, tumors were lysed in Super B buffer (Covaris, Cat# 520112) containing protease inhibitors, centrifuged, dried and re-constituted in acetonitrile, ammonium bicarbonate, pH 8.

    Protease Inhibitor:

    Article Title: Neuropilin-1 drives tumor-specific uptake of chlorotoxin
    Article Snippet: Tumors were cryofractured using the Cryoprep System (Covaris, Inc.), lysed 3:1 (v:w) in PBS containing 1x Halt™ Protease Inhibitor Cocktail (Thermo Scientific, Cat# 78430), and were extracted 3:1 with methanol:acetonitrile 1:1 (v:v) containing 0.1% acetic acid. .. Following cryofracture, tumors were lysed in Super B buffer (Covaris, Cat# 520112) containing protease inhibitors, centrifuged, dried and re-constituted in acetonitrile, ammonium bicarbonate, pH 8.

    Mass Spectrometry:

    Article Title: Neuropilin-1 drives tumor-specific uptake of chlorotoxin
    Article Snippet: ER-472 and S-methyl metabolite were then quantified using liquid chromatography coupled with tandem mass spectrometry analysis (LC-MS/MS). .. Following cryofracture, tumors were lysed in Super B buffer (Covaris, Cat# 520112) containing protease inhibitors, centrifuged, dried and re-constituted in acetonitrile, ammonium bicarbonate, pH 8.

    Liquid Chromatography:

    Article Title: Neuropilin-1 drives tumor-specific uptake of chlorotoxin
    Article Snippet: ER-472 and S-methyl metabolite were then quantified using liquid chromatography coupled with tandem mass spectrometry analysis (LC-MS/MS). .. Following cryofracture, tumors were lysed in Super B buffer (Covaris, Cat# 520112) containing protease inhibitors, centrifuged, dried and re-constituted in acetonitrile, ammonium bicarbonate, pH 8.

    Derivative Assay:

    Article Title: Neuropilin-1 drives tumor-specific uptake of chlorotoxin
    Article Snippet: To detect Cltx and derived peptides in PC-3 tumor lysate, Cltx peptide was administered at 150 mg/kg and tumors were harvested 1 h later. .. Following cryofracture, tumors were lysed in Super B buffer (Covaris, Cat# 520112) containing protease inhibitors, centrifuged, dried and re-constituted in acetonitrile, ammonium bicarbonate, pH 8.

    Software:

    Article Title: Neuropilin-1 drives tumor-specific uptake of chlorotoxin
    Article Snippet: Following cryofracture, tumors were lysed in Super B buffer (Covaris, Cat# 520112) containing protease inhibitors, centrifuged, dried and re-constituted in acetonitrile, ammonium bicarbonate, pH 8. .. BiopharmaLynx™ version 1.33 software (Waters) was used to analyze and identify Cltx-related peptides.

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