tissuetube tt05 25  (Covaris)


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    Name:
    tissueTUBE TT05 25
    Description:
    TissueTubes for sample collection storage pulverization and transfer For samples of mass 05 g For use with CP02 holder 500231 Case quantity available tissueTUBE TT05 Case 300 520102
    Catalog Number:
    520071
    Price:
    None
    Category:
    Consumable
    Quantity:
    1 0
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    Structured Review

    Covaris tissuetube tt05 25
    tissueTUBE TT05 25
    TissueTubes for sample collection storage pulverization and transfer For samples of mass 05 g For use with CP02 holder 500231 Case quantity available tissueTUBE TT05 Case 300 520102
    https://www.bioz.com/result/tissuetube tt05 25/product/Covaris
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    tissuetube tt05 25 - by Bioz Stars, 2020-10
    91/100 stars

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    Related Articles

    Sequencing:

    Article Title: Sex-specific differences in transcriptome profiles of brain and muscle tissue of the tropical gar
    Article Snippet: .. Library preparation and sequencing Each tissue was disrupted and homogenized by placing the sample in a CryoPrep tissueTUBE (Covaris #520071), freezing the sample in liquid nitrogen and then smashing with a mallet or using 2 millimeter tubes with Lysing Matrix D (1.4 mm) ceramic spheres (MP Biomedicals #116913050) on the Mini-Beadbeater-16 (BioSpec Products #607). .. Total RNA was isolated from the lysate using either the RNeasy Plus Mini Kit (Qiagen, #74104) or Nucleospin RNA kit (Macherey-Nagel #740698.5) (See Additional file : Table S1).

    other:

    Article Title: Oncogenic deregulation of EZH2 as an opportunity for targeted therapy in lung cancer
    Article Snippet: Lung tissue was smashed 1–2 times on setting 4 in the tissueTUBE (Covaris #520071).

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  • 99
    Covaris g tube device qc
    PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using <t>Covaris</t> <t>g-TUBE</t> and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures
    G Tube Device Qc, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g tube device qc/product/Covaris
    Average 99 stars, based on 43 article reviews
    Price from $9.99 to $1999.99
    g tube device qc - by Bioz Stars, 2020-10
    99/100 stars
      Buy from Supplier

    85
    Covaris covaris tissue tubes
    Dry pulverization method overview. Tissue is placed in <t>Covaris</t> tissueTUBES with adapters and attached glass vials (step 1). To keep samples both cold and brittle to facilitate pulverization, tissues are briefly submerged in liquid nitrogen (step 2) between successive rounds of pulverization (step 3). Following the pulverization of samples to a powder, the tissueTUBEs are inverted and tissue powder is collected into attached Covaris glass vials (step 4). Tissue powder is fixed, washed and stored as a pellet at −80°C.
    Covaris Tissue Tubes, supplied by Covaris, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/covaris tissue tubes/product/Covaris
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    covaris tissue tubes - by Bioz Stars, 2020-10
    85/100 stars
      Buy from Supplier

    Image Search Results


    PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using Covaris g-TUBE and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures

    Journal: Standards in Genomic Sciences

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

    doi: 10.1186/s40793-017-0239-1

    Figure Lengend Snippet: PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using Covaris g-TUBE and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures

    Article Snippet: The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes.

    Techniques: Magnetic Beads

    Purposeful mechanical shearing and high‐pass filtering alter DNA fragment length distribution. Pulsed‐field gel electrophoresis of differently treated DNA samples. Lane #1 and #5 (L) MidRange II PFG marker (BioLabs). Lane #2 (sample 10) DNA extracted following the default HMW DNA extraction protocol (mean read length of 13 kb as shown in Table 4 ). Lane #3 (sample 2) same DNA extraction as in #2 followed by size selection with the BluePippin using 20 kb high‐pass filtering (a mean read length of 26 kb as shown in Table 4 ). Lane #4 (sample 4) same DNA extraction as in #2 followed by mechanical shearing with a g‐TUBE (a mean read length of 11.8 kb as shown in Table 3 )

    Journal: Molecular Ecology Resources

    Article Title: Harnessing the MinION: An example of how to establish long‐read sequencing in a laboratory using challenging plant tissue from Eucalyptus pauciflora, et al. Harnessing the MinION: An example of how to establish long‐read sequencing in a laboratory using challenging plant tissue from Eucalyptus pauciflora

    doi: 10.1111/1755-0998.12938

    Figure Lengend Snippet: Purposeful mechanical shearing and high‐pass filtering alter DNA fragment length distribution. Pulsed‐field gel electrophoresis of differently treated DNA samples. Lane #1 and #5 (L) MidRange II PFG marker (BioLabs). Lane #2 (sample 10) DNA extracted following the default HMW DNA extraction protocol (mean read length of 13 kb as shown in Table 4 ). Lane #3 (sample 2) same DNA extraction as in #2 followed by size selection with the BluePippin using 20 kb high‐pass filtering (a mean read length of 26 kb as shown in Table 4 ). Lane #4 (sample 4) same DNA extraction as in #2 followed by mechanical shearing with a g‐TUBE (a mean read length of 11.8 kb as shown in Table 3 )

    Article Snippet: 2.3.1 g‐TUBE shearing We processed 5 µg of pure HMW DNA through a g‐TUBE (Covaris) in an Eppendorf 5,418 centrifuge at 3,800 rpm (1,243 g ), which is slightly lower than the recommended 4,200 rpm (1519 g) for 20 kb fragment size shearing, for a total of 2 min.

    Techniques: Pulsed-Field Gel, Electrophoresis, Marker, DNA Extraction, Selection

    Dry pulverization method overview. Tissue is placed in Covaris tissueTUBES with adapters and attached glass vials (step 1). To keep samples both cold and brittle to facilitate pulverization, tissues are briefly submerged in liquid nitrogen (step 2) between successive rounds of pulverization (step 3). Following the pulverization of samples to a powder, the tissueTUBEs are inverted and tissue powder is collected into attached Covaris glass vials (step 4). Tissue powder is fixed, washed and stored as a pellet at −80°C.

    Journal: Epigenetics & Chromatin

    Article Title: Mapping genome-wide transcription factor binding sites in frozen tissues

    doi: 10.1186/1756-8935-6-30

    Figure Lengend Snippet: Dry pulverization method overview. Tissue is placed in Covaris tissueTUBES with adapters and attached glass vials (step 1). To keep samples both cold and brittle to facilitate pulverization, tissues are briefly submerged in liquid nitrogen (step 2) between successive rounds of pulverization (step 3). Following the pulverization of samples to a powder, the tissueTUBEs are inverted and tissue powder is collected into attached Covaris glass vials (step 4). Tissue powder is fixed, washed and stored as a pellet at −80°C.

    Article Snippet: Frozen tissue samples (≈200 to 800 mg each) were pulverized in Covaris tissue TUBEs (Covaris 520001) with attached adapters (Covaris 520017) and glass vials (Covaris 520010).

    Techniques: