gtube  (Covaris)


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    Name:
    g TUBE 10
    Description:
    g TUBE is a single use device that shears genomic DNA into selected fragments sizes ranging from 6kb to 20kb The only equipment needed is a compatible bench top centrifuge 10 individually wrapped g TUBES for shearing DNA 6kb to 20 kb and one g TUBE Prep Station
    Catalog Number:
    520079
    Price:
    None
    Category:
    Consumable
    Quantity:
    10 0
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    Structured Review

    Covaris gtube
    g TUBE 10
    g TUBE is a single use device that shears genomic DNA into selected fragments sizes ranging from 6kb to 20kb The only equipment needed is a compatible bench top centrifuge 10 individually wrapped g TUBES for shearing DNA 6kb to 20 kb and one g TUBE Prep Station
    https://www.bioz.com/result/gtube/product/Covaris
    Average 95 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    gtube - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome
    Article Snippet: .. The sheared library was generated by centrifugation in a g-TUBE (Covaris part number 520079) at 1086 rcf. .. Library preparation was performed in parallel for both sheared and unsheared samples using Oxford Nanopore Technologies’ standard ligation sequencing kit, SQK-LSK109, using 1 μg of the appropriate genomic DNA (gDNA) and the standard protocol with a slight modification in that the final elution was done in 24 μl elution buffer instead of the standard 12 μl.

    Amplification:

    Article Title: Trichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters
    Article Snippet: The Illumina library (including PCR amplification and quantification) was prepared automatically by the NeoPrep Library Prep System, and then sequenced on the MiSeq platform (300 cycles, paired-end sequencing) with the MiSeq Control software version 2.5.1 and Sequencing Analysis Viewer version 1.8.20. .. High molecular weight DNA was sheared with g-TUBE (Covaris PN 520079), aiming at DNA fragments of about 20 kb.

    Lambda DNA Preparation:

    Article Title: Improved data analysis for the MinION nanopore sequencer
    Article Snippet: For BAC DNA, sequencing libraries were prepared using unshared DNA as well as DNA sheared to an average length of 10 kb using g-TUBE (Covaris, Cat. No. 520079). .. Briefly, the DNA sample was spiked with ONT lambda DNA control, end-repaired using NEBNext End Repair Module (NEB, Cat. No. E6050S), and cleaned up using Agencourt AMPure XP beads (Beckman Coulter; Cat. No. A63880).

    Article Title: A novel method for the multiplexed target enrichment of MinION next generation sequencing libraries using PCR-generated baits
    Article Snippet: .. Based on the hybridization method described below, we combined these 3 bait-pools with Phage Lambda DNA, after shearing it at approximately 5000 bp (‘5K’) and 10 000 bp (‘10K’) fragments with g-Tubes (520079, Covaris, Woburn, Massachusetts, USA), creating six combinations in total: S-5K, S-10K, M-5K, M-10K, L-5K, L-10K. .. Assessment of the enrichment We assessed the capture of the target sequences for each individual preparation with qPCR before the ligation of the sequencing adapters.

    Construct:

    Article Title: Trichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters
    Article Snippet: High molecular weight DNA was sheared with g-TUBE (Covaris PN 520079), aiming at DNA fragments of about 20 kb. .. The library was constructed with a 20 kb size-selected protocol using DNA Template Prep Kit 2.0 (PN 001-540-726), purified, and further selected for long insert size with a 0.35× AMPure (AMPure PB PN 100-265-900) bead size selection.

    Article Title: Long-read sequencing and de novo genome assembly of Ammopiptanthus nanus, a desert shrub
    Article Snippet: The SMRT Bell library was prepared using a DNA Template Prep Kit 1.0 (PacBio p/n 100-259-100), and four 20-kb SMRTbell libraries were constructed. .. Genomic DNA (10 µg) was mechanically sheared using a Covaris g-Tube (Kbiosciences p/n 520079) with a goal of DNA fragments of approximately 20 kb.

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: Illumina-compatible libraries were constructed with 1ug of sheared input DNA on a Zephyr Molecular Biology Workstation (Perkin Elmer, Waltham, MA) using custom scripts written to follow the manufacturer’s protocol for the NEBNext Ultra DNA Library Preparation Kit (cat. no. E7370, New England Biolabs, Ipswich, MA) combined with NEBNext Multiplex Oligo Index Primer Sets 1 and 2 (cat. no. E7335 and E7500). .. Library preparation for Pacific Biosciences (PacBio) RSII long-read sequencing (Menlo Park, CA) began by shearing 8 ug of purified gDNA to an average size of 15,000 bp using a Covaris g-tube (cat. no. 520079) as per the manufacturer’s instructions, followed by cleaning and concentration with pre-washed AMPure XP magnetic beads and suspension in Pacific Biosciences Elution Buffer.

    Incubation:

    Article Title: Picky Comprehensively Detects High Resolution Structural Variants in Nanopore Long Reads
    Article Snippet: Briefly, 1 × 106 frozen cells were lysed with 220 μL of Buffer ATL, 20 μL Proteinase K and incubated overnight at 56°C with 900 rpm. .. HMW genomic DNA was fragmented by either miniTUBE Blue (Covaris, 520065, for 3 Kb), miniTUBE Red (Covaris, 520066, for 5 Kb) or g-TUBE (Covaris, 520079, for 8 Kb and 12 Kb).

    Formalin-fixed Paraffin-Embedded:

    Article Title: Picky Comprehensively Detects High Resolution Structural Variants in Nanopore Long Reads
    Article Snippet: HMW genomic DNA was fragmented by either miniTUBE Blue (Covaris, 520065, for 3 Kb), miniTUBE Red (Covaris, 520066, for 5 Kb) or g-TUBE (Covaris, 520079, for 8 Kb and 12 Kb). .. Briefly, NEBNext FFPE RepairMix (NEB, M6630) was added to repair nicks in the DNA.

    Mass Spectrometry:

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: D), and loaded onto a MiSeq v2 Reagent Kit (cat. no. MS-102-2003) for 2 x 250 bp paired-end sequencing. .. Library preparation for Pacific Biosciences (PacBio) RSII long-read sequencing (Menlo Park, CA) began by shearing 8 ug of purified gDNA to an average size of 15,000 bp using a Covaris g-tube (cat. no. 520079) as per the manufacturer’s instructions, followed by cleaning and concentration with pre-washed AMPure XP magnetic beads and suspension in Pacific Biosciences Elution Buffer.

    Modification:

    Article Title: Extensive sequencing of seven human genomes to characterize benchmark reference materials
    Article Snippet: Paragraph title: AJ son modified manual library preparation ... The workflow to produce 5500 W DNA fragment libraries from AJ son human genomic DNA (gDNA) was as follows (also in ): Shearing of gDNA was performed using the Covaris g-Tube (PN 520079) in conjunction with the Covaris S2 Focused Ultrasonicator.

    Article Title: Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome
    Article Snippet: The sheared library was generated by centrifugation in a g-TUBE (Covaris part number 520079) at 1086 rcf. .. Library preparation was performed in parallel for both sheared and unsheared samples using Oxford Nanopore Technologies’ standard ligation sequencing kit, SQK-LSK109, using 1 μg of the appropriate genomic DNA (gDNA) and the standard protocol with a slight modification in that the final elution was done in 24 μl elution buffer instead of the standard 12 μl.

    Article Title: The genomes of Crithidia bombi and C. expoeki, common parasites of bumblebees
    Article Snippet: .. We produced one single-molecule real-time (SMRT) library for the PacBio platform according to the manufacturer's recommendations (Pacific Bioscience; but slightly modified, as we had to start with 10–20 μg, rather than 5 μg as suggested, sheared with g-tubes from Covaris, pn 520079), and then sequenced the library at the FGCZ on six SMRT cells and according to FGCZ's protocols. ..

    Hybridization:

    Article Title: A novel method for the multiplexed target enrichment of MinION next generation sequencing libraries using PCR-generated baits
    Article Snippet: .. Based on the hybridization method described below, we combined these 3 bait-pools with Phage Lambda DNA, after shearing it at approximately 5000 bp (‘5K’) and 10 000 bp (‘10K’) fragments with g-Tubes (520079, Covaris, Woburn, Massachusetts, USA), creating six combinations in total: S-5K, S-10K, M-5K, M-10K, L-5K, L-10K. .. Assessment of the enrichment We assessed the capture of the target sequences for each individual preparation with qPCR before the ligation of the sequencing adapters.

    Flow Cytometry:

    Article Title: Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome
    Article Snippet: The sheared library was generated by centrifugation in a g-TUBE (Covaris part number 520079) at 1086 rcf. .. The 24 μl of library was combined with 75 μl sequencing buffer and 51 μl of loading beads and run on a PromethION Beta with PromethION flow cell version 3 using MinKNOW software version 1.11.9 for 64 h. Base-calling was performed using Guppy version 1.4.0 and using a manually set chunk size of 10 kb.

    Ligation:

    Article Title: SMRT sequencing of the Campylobacter coli BfR-CA-9557 genome sequence reveals unique methylation motifs
    Article Snippet: 10 μg of gDNA were mechanically sheared to an avarage size distribution of 10Kb, using a Covaris gTube (Kbiosciences, Hoddesdon, UK; p/n 520079). .. A blunt end ligation reaction followed by exonuclease treatment was performed to create the SMRT bell template.

    Article Title: Multiple hybrid de novo genome assembly of finger millet, an orphan allotetraploid crop
    Article Snippet: The concentration of the input gDNA was measured using a Qubit Fluorometer dsDNA Broad Range assay (Life Technologies p/n 32850). gDNA (10 µg) was mechanically sheared to an average size distribution of 10 kb using a Covaris gTube (Kbiosciences p/n 520079). .. A blunt-end ligation reaction followed by exonuclease treatment was performed to create the SMRT Bell template.

    Article Title: Long-read sequencing and de novo genome assembly of Ammopiptanthus nanus, a desert shrub
    Article Snippet: Genomic DNA (10 µg) was mechanically sheared using a Covaris g-Tube (Kbiosciences p/n 520079) with a goal of DNA fragments of approximately 20 kb. .. A blunt-end ligation reaction followed by exonuclease treatment was conducted to generate the SMRT Bell template.

    Article Title: New insights on Pseudoalteromonas haloplanktis TAC125 genome organization and benchmarks of genome assembly applications using next and third generation sequencing technologies
    Article Snippet: 10 μg of gDNA were mechanically sheared to an average size distribution of 15-20 kb, using a Covaris gTube (Covaris p/n 520079). .. A ligation reaction was performed to create the SMRT bell template, according to the manufacturer’s instructions.

    Article Title: Identification and characterization of an IgG sequence variant with an 11 kDa heavy chain C-terminal extension using a combination of mass spectrometry and high-throughput sequencing analysis
    Article Snippet: 1.5 µg DNA was sheared using a G-tube (Covaris, Woburn, MA, USA, Cat# 520079) at 6000 RPM to generate fragments of ~8 kb. .. DNA was sequenced using a MinION instrument (Oxford Nanopore, Oxford, UK) using the 1D sequencing by ligation method, generating 915698 reads covering ~6035 megabases (approximately 2.3-fold genome coverage).

    Article Title: Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome
    Article Snippet: The sheared library was generated by centrifugation in a g-TUBE (Covaris part number 520079) at 1086 rcf. .. Library preparation was performed in parallel for both sheared and unsheared samples using Oxford Nanopore Technologies’ standard ligation sequencing kit, SQK-LSK109, using 1 μg of the appropriate genomic DNA (gDNA) and the standard protocol with a slight modification in that the final elution was done in 24 μl elution buffer instead of the standard 12 μl.

    Article Title: Improved data analysis for the MinION nanopore sequencer
    Article Snippet: For BAC DNA, sequencing libraries were prepared using unshared DNA as well as DNA sheared to an average length of 10 kb using g-TUBE (Covaris, Cat. No. 520079). .. This was followed by ligation of ONT sequencing adaptors (adaptor Mix and HP adaptor) using Blunt/TA Ligase Master Mix (NEB, Cat. No. M0367S).

    Biomarker Assay:

    Article Title: Long-read sequencing and de novo genome assembly of Ammopiptanthus nanus, a desert shrub
    Article Snippet: Long-read sequencing was performed at Biomarker Technologies Corporation (Beijing, China) with a PacBio Sequel sequencer (Pacific Biosciences, Menlo Park, CA, USA). .. Genomic DNA (10 µg) was mechanically sheared using a Covaris g-Tube (Kbiosciences p/n 520079) with a goal of DNA fragments of approximately 20 kb.

    Generated:

    Article Title: Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome
    Article Snippet: .. The sheared library was generated by centrifugation in a g-TUBE (Covaris part number 520079) at 1086 rcf. .. Library preparation was performed in parallel for both sheared and unsheared samples using Oxford Nanopore Technologies’ standard ligation sequencing kit, SQK-LSK109, using 1 μg of the appropriate genomic DNA (gDNA) and the standard protocol with a slight modification in that the final elution was done in 24 μl elution buffer instead of the standard 12 μl.

    Article Title: A novel method for the multiplexed target enrichment of MinION next generation sequencing libraries using PCR-generated baits
    Article Snippet: Baits’ length and genomic DNA shearing optimization We generated the baits used in this study through multiple PCR reactions. .. Based on the hybridization method described below, we combined these 3 bait-pools with Phage Lambda DNA, after shearing it at approximately 5000 bp (‘5K’) and 10 000 bp (‘10K’) fragments with g-Tubes (520079, Covaris, Woburn, Massachusetts, USA), creating six combinations in total: S-5K, S-10K, M-5K, M-10K, L-5K, L-10K.

    DNA Sequencing:

    Article Title: Trichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters
    Article Snippet: Paragraph title: DNA sequencing and de novo assembly ... High molecular weight DNA was sheared with g-TUBE (Covaris PN 520079), aiming at DNA fragments of about 20 kb.

    Article Title: Long-read sequencing and de novo genome assembly of Ammopiptanthus nanus, a desert shrub
    Article Snippet: Paragraph title: Sample collection and genomic DNA sequencing ... Genomic DNA (10 µg) was mechanically sheared using a Covaris g-Tube (Kbiosciences p/n 520079) with a goal of DNA fragments of approximately 20 kb.

    Article Title: Improved data analysis for the MinION nanopore sequencer
    Article Snippet: .. For BAC DNA, sequencing libraries were prepared using unshared DNA as well as DNA sheared to an average length of 10 kb using g-TUBE (Covaris, Cat. No. 520079). .. Briefly, the DNA sample was spiked with ONT lambda DNA control, end-repaired using NEBNext End Repair Module (NEB, Cat. No. E6050S), and cleaned up using Agencourt AMPure XP beads (Beckman Coulter; Cat. No. A63880).

    Polymerase Chain Reaction:

    Article Title: Trichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters
    Article Snippet: The Illumina library (including PCR amplification and quantification) was prepared automatically by the NeoPrep Library Prep System, and then sequenced on the MiSeq platform (300 cycles, paired-end sequencing) with the MiSeq Control software version 2.5.1 and Sequencing Analysis Viewer version 1.8.20. .. High molecular weight DNA was sheared with g-TUBE (Covaris PN 520079), aiming at DNA fragments of about 20 kb.

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: NEB USER enzyme was included during the PCR cycling step, with a subsequent AMPure magnetic bead cleanup (at 0.7X) and elution into Tris-HCl, pH 8.0. .. Library preparation for Pacific Biosciences (PacBio) RSII long-read sequencing (Menlo Park, CA) began by shearing 8 ug of purified gDNA to an average size of 15,000 bp using a Covaris g-tube (cat. no. 520079) as per the manufacturer’s instructions, followed by cleaning and concentration with pre-washed AMPure XP magnetic beads and suspension in Pacific Biosciences Elution Buffer.

    Article Title: A novel method for the multiplexed target enrichment of MinION next generation sequencing libraries using PCR-generated baits
    Article Snippet: Baits’ length and genomic DNA shearing optimization We generated the baits used in this study through multiple PCR reactions. .. Based on the hybridization method described below, we combined these 3 bait-pools with Phage Lambda DNA, after shearing it at approximately 5000 bp (‘5K’) and 10 000 bp (‘10K’) fragments with g-Tubes (520079, Covaris, Woburn, Massachusetts, USA), creating six combinations in total: S-5K, S-10K, M-5K, M-10K, L-5K, L-10K.

    Binding Assay:

    Article Title: Trichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters
    Article Snippet: High molecular weight DNA was sheared with g-TUBE (Covaris PN 520079), aiming at DNA fragments of about 20 kb. .. The library was sequenced on a PacBio RSII device using the reagents DNA/Polymerase Binding Kit P4 (PN 100-236-500), DNA Sequencing Reagent 2.0 PN (100-216-400), SMRT® Cell V3 (PN 100-171-800), and MagBead (PN 100-133-600), loading at 200 pM on the plate.

    Molecular Weight:

    Article Title: Trichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters
    Article Snippet: .. High molecular weight DNA was sheared with g-TUBE (Covaris PN 520079), aiming at DNA fragments of about 20 kb. .. The library was constructed with a 20 kb size-selected protocol using DNA Template Prep Kit 2.0 (PN 001-540-726), purified, and further selected for long insert size with a 0.35× AMPure (AMPure PB PN 100-265-900) bead size selection.

    DNA Extraction:

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: Paragraph title: Genomic DNA Extraction and NGS Library Preparation ... Library preparation for Pacific Biosciences (PacBio) RSII long-read sequencing (Menlo Park, CA) began by shearing 8 ug of purified gDNA to an average size of 15,000 bp using a Covaris g-tube (cat. no. 520079) as per the manufacturer’s instructions, followed by cleaning and concentration with pre-washed AMPure XP magnetic beads and suspension in Pacific Biosciences Elution Buffer.

    Magnetic Beads:

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: .. Library preparation for Pacific Biosciences (PacBio) RSII long-read sequencing (Menlo Park, CA) began by shearing 8 ug of purified gDNA to an average size of 15,000 bp using a Covaris g-tube (cat. no. 520079) as per the manufacturer’s instructions, followed by cleaning and concentration with pre-washed AMPure XP magnetic beads and suspension in Pacific Biosciences Elution Buffer. .. Average sheared fragment size was verified on an Agilent Tapestation 2200 using the Genomic DNA ScreenTape and reagents (cat. no. 5067–5365 and 5067–5366) and quality parameters were measured by a Nanodrop ND-1000 spectrophotometer.

    Isolation:

    Article Title: Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome
    Article Snippet: Both libraries were generated from 5-μg genomic DNA isolated from blood. .. The sheared library was generated by centrifugation in a g-TUBE (Covaris part number 520079) at 1086 rcf.

    Functional Assay:

    Article Title: SMRT sequencing of the Campylobacter coli BfR-CA-9557 genome sequence reveals unique methylation motifs
    Article Snippet: The DNA libraries have been prepared following the PacBio guidelines and sequenced on a SMRT cell using Pacific Biosciences RS sequencing technology (Pacific Biosciences, Menlo Park, USA) at Functional Genomics Center Zürich (FGCZ, Switzerland). .. 10 μg of gDNA were mechanically sheared to an avarage size distribution of 10Kb, using a Covaris gTube (Kbiosciences, Hoddesdon, UK; p/n 520079).

    Article Title: The genomes of Crithidia bombi and C. expoeki, common parasites of bumblebees
    Article Snippet: Genome sequencing We sequenced the full genome of Crithidia bombi using a combination of sequencing runs on the Roche 454 FLX Titanium, Pacific Biosciences PacBio RS (starting in 2009; both at the Functional Genomics Center Zurich, FGCZ; http://www.fgcz.ch ), and Illumina GA2 at GATC-Biotech (Konstanz, Germany). .. We produced one single-molecule real-time (SMRT) library for the PacBio platform according to the manufacturer's recommendations (Pacific Bioscience; but slightly modified, as we had to start with 10–20 μg, rather than 5 μg as suggested, sheared with g-tubes from Covaris, pn 520079), and then sequenced the library at the FGCZ on six SMRT cells and according to FGCZ's protocols.

    Purification:

    Article Title: Trichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters
    Article Snippet: High molecular weight DNA was sheared with g-TUBE (Covaris PN 520079), aiming at DNA fragments of about 20 kb. .. The library was constructed with a 20 kb size-selected protocol using DNA Template Prep Kit 2.0 (PN 001-540-726), purified, and further selected for long insert size with a 0.35× AMPure (AMPure PB PN 100-265-900) bead size selection.

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: .. Library preparation for Pacific Biosciences (PacBio) RSII long-read sequencing (Menlo Park, CA) began by shearing 8 ug of purified gDNA to an average size of 15,000 bp using a Covaris g-tube (cat. no. 520079) as per the manufacturer’s instructions, followed by cleaning and concentration with pre-washed AMPure XP magnetic beads and suspension in Pacific Biosciences Elution Buffer. .. Average sheared fragment size was verified on an Agilent Tapestation 2200 using the Genomic DNA ScreenTape and reagents (cat. no. 5067–5365 and 5067–5366) and quality parameters were measured by a Nanodrop ND-1000 spectrophotometer.

    Article Title: Improved data analysis for the MinION nanopore sequencer
    Article Snippet: For BAC DNA, sequencing libraries were prepared using unshared DNA as well as DNA sheared to an average length of 10 kb using g-TUBE (Covaris, Cat. No. 520079). .. The purified end-repaired DNA then underwent dA tailing using the NEB dA-Tailing Module (NEB, Cat. No. E6053S).

    Sequencing:

    Article Title: SMRT sequencing of the Campylobacter coli BfR-CA-9557 genome sequence reveals unique methylation motifs
    Article Snippet: Paragraph title: Library Preparation and Sequencing ... 10 μg of gDNA were mechanically sheared to an avarage size distribution of 10Kb, using a Covaris gTube (Kbiosciences, Hoddesdon, UK; p/n 520079).

    Article Title: Trichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters
    Article Snippet: Sequencing was coordinated by Carolina Correa and Tonia Russel. .. High molecular weight DNA was sheared with g-TUBE (Covaris PN 520079), aiming at DNA fragments of about 20 kb.

    Article Title: Multiple hybrid de novo genome assembly of finger millet, an orphan allotetraploid crop
    Article Snippet: Paragraph title: Library preparation and sequencing for PacBio RS II ... The concentration of the input gDNA was measured using a Qubit Fluorometer dsDNA Broad Range assay (Life Technologies p/n 32850). gDNA (10 µg) was mechanically sheared to an average size distribution of 10 kb using a Covaris gTube (Kbiosciences p/n 520079).

    Article Title: Long-read sequencing and de novo genome assembly of Ammopiptanthus nanus, a desert shrub
    Article Snippet: Long-read sequencing was performed at Biomarker Technologies Corporation (Beijing, China) with a PacBio Sequel sequencer (Pacific Biosciences, Menlo Park, CA, USA). .. Genomic DNA (10 µg) was mechanically sheared using a Covaris g-Tube (Kbiosciences p/n 520079) with a goal of DNA fragments of approximately 20 kb.

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: .. Library preparation for Pacific Biosciences (PacBio) RSII long-read sequencing (Menlo Park, CA) began by shearing 8 ug of purified gDNA to an average size of 15,000 bp using a Covaris g-tube (cat. no. 520079) as per the manufacturer’s instructions, followed by cleaning and concentration with pre-washed AMPure XP magnetic beads and suspension in Pacific Biosciences Elution Buffer. .. Average sheared fragment size was verified on an Agilent Tapestation 2200 using the Genomic DNA ScreenTape and reagents (cat. no. 5067–5365 and 5067–5366) and quality parameters were measured by a Nanodrop ND-1000 spectrophotometer.

    Article Title: Identification and characterization of an IgG sequence variant with an 11 kDa heavy chain C-terminal extension using a combination of mass spectrometry and high-throughput sequencing analysis
    Article Snippet: Paragraph title: MinION sequencing and bioinformatic analysis ... 1.5 µg DNA was sheared using a G-tube (Covaris, Woburn, MA, USA, Cat# 520079) at 6000 RPM to generate fragments of ~8 kb.

    Article Title: Picky Comprehensively Detects High Resolution Structural Variants in Nanopore Long Reads
    Article Snippet: Paragraph title: Nanopore long read sequencing ... HMW genomic DNA was fragmented by either miniTUBE Blue (Covaris, 520065, for 3 Kb), miniTUBE Red (Covaris, 520066, for 5 Kb) or g-TUBE (Covaris, 520079, for 8 Kb and 12 Kb).

    Article Title: Extensive sequencing of seven human genomes to characterize benchmark reference materials
    Article Snippet: AJ son modified manual library preparation A modified manual library preparation process for the AJ son was used to obtain appropriately sized libraries for sequencing on a 5500×l Wildfire. .. The workflow to produce 5500 W DNA fragment libraries from AJ son human genomic DNA (gDNA) was as follows (also in ): Shearing of gDNA was performed using the Covaris g-Tube (PN 520079) in conjunction with the Covaris S2 Focused Ultrasonicator.

    Article Title: Extensive sequencing of seven human genomes to characterize benchmark reference materials
    Article Snippet: SMRTbell library preparation of AJ Trio gDNA DNA library preparation and sequencing was performed according to the manufacturer’s instructions with noted modifications. .. The 150 μl aliquots were individually pipetted into the top chambers of Covaris G-tube (PN# 520079) spin columns and sheared for 60 s at 4500 rpm using an Eppendorf 5424 benchtop centrifuge.

    Article Title: Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome
    Article Snippet: Paragraph title: Sample preparation, sequencing, and base-calling ... The sheared library was generated by centrifugation in a g-TUBE (Covaris part number 520079) at 1086 rcf.

    Article Title: Improved data analysis for the MinION nanopore sequencer
    Article Snippet: Paragraph title: M13 MinION sequencing ... For BAC DNA, sequencing libraries were prepared using unshared DNA as well as DNA sheared to an average length of 10 kb using g-TUBE (Covaris, Cat. No. 520079).

    Article Title: Extensive sequencing of seven human genomes to characterize benchmark reference materials
    Article Snippet: Chinese son semi-automated library preparation A semi-automated library preparation using the AB Library Builder System was used to prepare Chinese son libraries for sequencing on a 5500×l Wildfire. .. The workflow to produce 5500 W DNA fragment libraries from Chinese son human genomic DNA (gDNA) was as follows (also in ): Shearing of gDNA was performed using the Covaris g-Tube (PN 520079) in conjunction with the Covaris S2 Focused Ultrasonicator.

    Article Title: The genomes of Crithidia bombi and C. expoeki, common parasites of bumblebees
    Article Snippet: Paragraph title: Genome sequencing ... We produced one single-molecule real-time (SMRT) library for the PacBio platform according to the manufacturer's recommendations (Pacific Bioscience; but slightly modified, as we had to start with 10–20 μg, rather than 5 μg as suggested, sheared with g-tubes from Covaris, pn 520079), and then sequenced the library at the FGCZ on six SMRT cells and according to FGCZ's protocols.

    Sample Prep:

    Article Title: Trichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters
    Article Snippet: A shotgun paired-end library (average size of 550 bp) was prepared using the Illumina TruSeq DNA nano Sample Prep Kit. .. High molecular weight DNA was sheared with g-TUBE (Covaris PN 520079), aiming at DNA fragments of about 20 kb.

    Article Title: Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome
    Article Snippet: Paragraph title: Sample preparation, sequencing, and base-calling ... The sheared library was generated by centrifugation in a g-TUBE (Covaris part number 520079) at 1086 rcf.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Identification and characterization of an IgG sequence variant with an 11 kDa heavy chain C-terminal extension using a combination of mass spectrometry and high-throughput sequencing analysis
    Article Snippet: MinION sequencing and bioinformatic analysis Genomic DNA was extracted from cells using PureLink Genomic DNA Mini Kit (Thermo Fisher Scientific, CatK182001). .. 1.5 µg DNA was sheared using a G-tube (Covaris, Woburn, MA, USA, Cat# 520079) at 6000 RPM to generate fragments of ~8 kb.

    Chromatin Immunoprecipitation:

    Article Title: SMRT sequencing of the Campylobacter coli BfR-CA-9557 genome sequence reveals unique methylation motifs
    Article Snippet: 10 μg of gDNA were mechanically sheared to an avarage size distribution of 10Kb, using a Covaris gTube (Kbiosciences, Hoddesdon, UK; p/n 520079). .. A Bioanalyzer 2100 12 K DNA Chip assay (Agilent Technologies, Santa Clara, USA; p/n 5067–1508) was used to assess the fragment size distribution.

    Article Title: Multiple hybrid de novo genome assembly of finger millet, an orphan allotetraploid crop
    Article Snippet: The concentration of the input gDNA was measured using a Qubit Fluorometer dsDNA Broad Range assay (Life Technologies p/n 32850). gDNA (10 µg) was mechanically sheared to an average size distribution of 10 kb using a Covaris gTube (Kbiosciences p/n 520079). .. A Bioanalyzer 2100 12K DNA Chip assay (Agilent p/n 5067-1508) was used to assess the fragment size distribution.

    Article Title: Long-read sequencing and de novo genome assembly of Ammopiptanthus nanus, a desert shrub
    Article Snippet: Genomic DNA (10 µg) was mechanically sheared using a Covaris g-Tube (Kbiosciences p/n 520079) with a goal of DNA fragments of approximately 20 kb. .. A Bioanalyzer 2100 12K DNA Chip assay (Agilent p/n 5067-1508) was used to assess the fragment size distribution.

    Plasmid Preparation:

    Article Title: Identification and characterization of an IgG sequence variant with an 11 kDa heavy chain C-terminal extension using a combination of mass spectrometry and high-throughput sequencing analysis
    Article Snippet: 1.5 µg DNA was sheared using a G-tube (Covaris, Woburn, MA, USA, Cat# 520079) at 6000 RPM to generate fragments of ~8 kb. .. The reads were then compared against the linear plasmid sequence using blastn using parameters suitable for nanopore data “blastn -reward 5 -penalty −4 -gapopen 8 -gapextend 6 -dust no”.

    Software:

    Article Title: Trichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters
    Article Snippet: The Illumina library (including PCR amplification and quantification) was prepared automatically by the NeoPrep Library Prep System, and then sequenced on the MiSeq platform (300 cycles, paired-end sequencing) with the MiSeq Control software version 2.5.1 and Sequencing Analysis Viewer version 1.8.20. .. High molecular weight DNA was sheared with g-TUBE (Covaris PN 520079), aiming at DNA fragments of about 20 kb.

    Article Title: Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome
    Article Snippet: The sheared library was generated by centrifugation in a g-TUBE (Covaris part number 520079) at 1086 rcf. .. The 24 μl of library was combined with 75 μl sequencing buffer and 51 μl of loading beads and run on a PromethION Beta with PromethION flow cell version 3 using MinKNOW software version 1.11.9 for 64 h. Base-calling was performed using Guppy version 1.4.0 and using a manually set chunk size of 10 kb.

    Multiplex Assay:

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: Illumina-compatible libraries were constructed with 1ug of sheared input DNA on a Zephyr Molecular Biology Workstation (Perkin Elmer, Waltham, MA) using custom scripts written to follow the manufacturer’s protocol for the NEBNext Ultra DNA Library Preparation Kit (cat. no. E7370, New England Biolabs, Ipswich, MA) combined with NEBNext Multiplex Oligo Index Primer Sets 1 and 2 (cat. no. E7335 and E7500). .. Library preparation for Pacific Biosciences (PacBio) RSII long-read sequencing (Menlo Park, CA) began by shearing 8 ug of purified gDNA to an average size of 15,000 bp using a Covaris g-tube (cat. no. 520079) as per the manufacturer’s instructions, followed by cleaning and concentration with pre-washed AMPure XP magnetic beads and suspension in Pacific Biosciences Elution Buffer.

    Selection:

    Article Title: Trichoderma reesei complete genome sequence, repeat-induced point mutation, and partitioning of CAZyme gene clusters
    Article Snippet: High molecular weight DNA was sheared with g-TUBE (Covaris PN 520079), aiming at DNA fragments of about 20 kb. .. The library was constructed with a 20 kb size-selected protocol using DNA Template Prep Kit 2.0 (PN 001-540-726), purified, and further selected for long insert size with a 0.35× AMPure (AMPure PB PN 100-265-900) bead size selection.

    Article Title: Picky Comprehensively Detects High Resolution Structural Variants in Nanopore Long Reads
    Article Snippet: HMW genomic DNA was fragmented by either miniTUBE Blue (Covaris, 520065, for 3 Kb), miniTUBE Red (Covaris, 520066, for 5 Kb) or g-TUBE (Covaris, 520079, for 8 Kb and 12 Kb). .. For libraries targeted at 12 Kb, size selection was performed for sheared fragments larger than 10 Kb using 0.75% agarose cassette (Sage Science, BLF7510) by Blue Pippin™ DNA Size Selection System.

    Agarose Gel Electrophoresis:

    Article Title: Long-read sequencing and de novo genome assembly of Ammopiptanthus nanus, a desert shrub
    Article Snippet: The quality of the extracted genomic DNA was checked using 1% agarose gel electrophoresis, and the concentration was quantified using a Qubit fluorimeter (Invitrogen, Carlsbad, CA, USA). .. Genomic DNA (10 µg) was mechanically sheared using a Covaris g-Tube (Kbiosciences p/n 520079) with a goal of DNA fragments of approximately 20 kb.

    Next-Generation Sequencing:

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: Paragraph title: Genomic DNA Extraction and NGS Library Preparation ... Library preparation for Pacific Biosciences (PacBio) RSII long-read sequencing (Menlo Park, CA) began by shearing 8 ug of purified gDNA to an average size of 15,000 bp using a Covaris g-tube (cat. no. 520079) as per the manufacturer’s instructions, followed by cleaning and concentration with pre-washed AMPure XP magnetic beads and suspension in Pacific Biosciences Elution Buffer.

    dsDNA Assay:

    Article Title: Extensive sequencing of seven human genomes to characterize benchmark reference materials
    Article Snippet: Genomic DNA extracts were verified with the Life Technologies Qubit 2.0 Fluorometer using the High Sensitivity dsDNA assay (PN# Q32851) to quantify the mass of double-stranded DNA present. .. The 150 μl aliquots were individually pipetted into the top chambers of Covaris G-tube (PN# 520079) spin columns and sheared for 60 s at 4500 rpm using an Eppendorf 5424 benchtop centrifuge.

    Spectrophotometry:

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: DNA quality (260:280 and 260:230) was measured on a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE) and initial average fragment size was verified on the Agilent Tapestation 2200 (Santa Clara, CA) using the D1000 ScreenTape and reagents (cat. no. 5067–5582 and 5067–5583) with an external ladder. .. Library preparation for Pacific Biosciences (PacBio) RSII long-read sequencing (Menlo Park, CA) began by shearing 8 ug of purified gDNA to an average size of 15,000 bp using a Covaris g-tube (cat. no. 520079) as per the manufacturer’s instructions, followed by cleaning and concentration with pre-washed AMPure XP magnetic beads and suspension in Pacific Biosciences Elution Buffer.

    Produced:

    Article Title: SMRT sequencing of the Campylobacter coli BfR-CA-9557 genome sequence reveals unique methylation motifs
    Article Snippet: The SMRT bell was produced using the DNA Template Prep Kit 1.0 (Pacific Biosciences; p/n 100-259-100). .. 10 μg of gDNA were mechanically sheared to an avarage size distribution of 10Kb, using a Covaris gTube (Kbiosciences, Hoddesdon, UK; p/n 520079).

    Article Title: Multiple hybrid de novo genome assembly of finger millet, an orphan allotetraploid crop
    Article Snippet: Library preparation and sequencing for PacBio RS II The SMRT Bell library was produced using a DNA Template Prep Kit 1.0 (PacBio p/n 100-259-100). .. The concentration of the input gDNA was measured using a Qubit Fluorometer dsDNA Broad Range assay (Life Technologies p/n 32850). gDNA (10 µg) was mechanically sheared to an average size distribution of 10 kb using a Covaris gTube (Kbiosciences p/n 520079).

    Article Title: New insights on Pseudoalteromonas haloplanktis TAC125 genome organization and benchmarks of genome assembly applications using next and third generation sequencing technologies
    Article Snippet: PacBio RSII A SMRT bell library was produced using the SMRTbell Express Template Prep Kit (Pacific Biosciences 101-357-000). .. 10 μg of gDNA were mechanically sheared to an average size distribution of 15-20 kb, using a Covaris gTube (Covaris p/n 520079).

    Article Title: A novel method for the multiplexed target enrichment of MinION next generation sequencing libraries using PCR-generated baits
    Article Snippet: Twelve primers (Sigma Aldrich, Gillingham, UK) (primers L3F to L5R in Table ), in combination with Platinum Taq DNA Polymerase (10966–018, Invitrogen, Carlsbad, CA) produced 6 baits of average length 242 bp (short-‘S’), or 3 baits of average length 460 bp (medium-‘M’) or 2 baits of average length 884 bp (long-‘L’) (Supplementary Figure S2). .. Based on the hybridization method described below, we combined these 3 bait-pools with Phage Lambda DNA, after shearing it at approximately 5000 bp (‘5K’) and 10 000 bp (‘10K’) fragments with g-Tubes (520079, Covaris, Woburn, Massachusetts, USA), creating six combinations in total: S-5K, S-10K, M-5K, M-10K, L-5K, L-10K.

    Article Title: The genomes of Crithidia bombi and C. expoeki, common parasites of bumblebees
    Article Snippet: .. We produced one single-molecule real-time (SMRT) library for the PacBio platform according to the manufacturer's recommendations (Pacific Bioscience; but slightly modified, as we had to start with 10–20 μg, rather than 5 μg as suggested, sheared with g-tubes from Covaris, pn 520079), and then sequenced the library at the FGCZ on six SMRT cells and according to FGCZ's protocols. ..

    Concentration Assay:

    Article Title: SMRT sequencing of the Campylobacter coli BfR-CA-9557 genome sequence reveals unique methylation motifs
    Article Snippet: Input genomic DNA concentration was measured using a Qubit Fluorometer dsDNA Broad Range assay (Life Technologies, Carlsbad, USA; p/n 32850). .. 10 μg of gDNA were mechanically sheared to an avarage size distribution of 10Kb, using a Covaris gTube (Kbiosciences, Hoddesdon, UK; p/n 520079).

    Article Title: Multiple hybrid de novo genome assembly of finger millet, an orphan allotetraploid crop
    Article Snippet: .. The concentration of the input gDNA was measured using a Qubit Fluorometer dsDNA Broad Range assay (Life Technologies p/n 32850). gDNA (10 µg) was mechanically sheared to an average size distribution of 10 kb using a Covaris gTube (Kbiosciences p/n 520079). .. A Bioanalyzer 2100 12K DNA Chip assay (Agilent p/n 5067-1508) was used to assess the fragment size distribution.

    Article Title: Long-read sequencing and de novo genome assembly of Ammopiptanthus nanus, a desert shrub
    Article Snippet: The quality of the extracted genomic DNA was checked using 1% agarose gel electrophoresis, and the concentration was quantified using a Qubit fluorimeter (Invitrogen, Carlsbad, CA, USA). .. Genomic DNA (10 µg) was mechanically sheared using a Covaris g-Tube (Kbiosciences p/n 520079) with a goal of DNA fragments of approximately 20 kb.

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: .. Library preparation for Pacific Biosciences (PacBio) RSII long-read sequencing (Menlo Park, CA) began by shearing 8 ug of purified gDNA to an average size of 15,000 bp using a Covaris g-tube (cat. no. 520079) as per the manufacturer’s instructions, followed by cleaning and concentration with pre-washed AMPure XP magnetic beads and suspension in Pacific Biosciences Elution Buffer. .. Average sheared fragment size was verified on an Agilent Tapestation 2200 using the Genomic DNA ScreenTape and reagents (cat. no. 5067–5365 and 5067–5366) and quality parameters were measured by a Nanodrop ND-1000 spectrophotometer.

    BAC Assay:

    Article Title: Improved data analysis for the MinION nanopore sequencer
    Article Snippet: .. For BAC DNA, sequencing libraries were prepared using unshared DNA as well as DNA sheared to an average length of 10 kb using g-TUBE (Covaris, Cat. No. 520079). .. Briefly, the DNA sample was spiked with ONT lambda DNA control, end-repaired using NEBNext End Repair Module (NEB, Cat. No. E6050S), and cleaned up using Agencourt AMPure XP beads (Beckman Coulter; Cat. No. A63880).

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    Covaris g tube device qc
    PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using <t>Covaris</t> <t>g-TUBE</t> and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures
    G Tube Device Qc, supplied by Covaris, used in various techniques. Bioz Stars score: 95/100, based on 170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/g tube device qc/product/Covaris
    Average 95 stars, based on 170 article reviews
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    g tube device qc - by Bioz Stars, 2020-02
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    82
    Covaris covaris tissue tubes
    Dry pulverization method overview. Tissue is placed in <t>Covaris</t> tissueTUBES with adapters and attached glass vials (step 1). To keep samples both cold and brittle to facilitate pulverization, tissues are briefly submerged in liquid nitrogen (step 2) between successive rounds of pulverization (step 3). Following the pulverization of samples to a powder, the tissueTUBEs are inverted and tissue powder is collected into attached Covaris glass vials (step 4). Tissue powder is fixed, washed and stored as a pellet at −80°C.
    Covaris Tissue Tubes, supplied by Covaris, used in various techniques. Bioz Stars score: 82/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/covaris tissue tubes/product/Covaris
    Average 82 stars, based on 2 article reviews
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    Image Search Results


    PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using Covaris g-TUBE and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures

    Journal: Standards in Genomic Sciences

    Article Title: Automation of PacBio SMRTbell NGS library preparation for bacterial genome sequencing

    doi: 10.1186/s40793-017-0239-1

    Figure Lengend Snippet: PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. PacBio SMRTbell Template Preparation Workflow for PacBio RS II system. This workflow is used to prepare libraries from fragmented and concentrated DNA using Covaris g-TUBE and concentrated using the AMPure magnetic beads before following PacBio SMRTbell 10 kb Library Preparation procedures

    Article Snippet: The initial evaluation of the quantity and size distribution of the purified gDNA was with the Agilent 2200 TapeStation Nucleic Acid System (G2965AA) controlled by Agilent 2200 TapeStation Software A.01.05, using the Agilent Genomic DNA ScreenTape (5067–5365) and the Agilent Genomic DNA Reagents (5067–5366) with samples drawn from a 96-well plate [ , ] Determination of the quality of the gDNA Fragment gDNA using a Covaris g-TUBE device QC the sizing and adjust the concentration Repair DNA damage and repair ends of fragmented DNA Purify the DNA Blunt-end ligate using blunt adapters Purify template for submission to a sequencer In Fig. , A (Post Shearing Clean-up) and B (10kb Library Prep Runset Dual SPRI) are two of the VWorks protocol graphical user interfaces that help with the NGS Workstation setup and deck layout to optimize the use of reagent volumes.

    Techniques: Magnetic Beads

    The pipeline of Fosmid-size long paired-end library construction. The red area represents the vector, the blue area represents the large inserted genomic fragment, and the yellow area represents the Ampicillin resistance gene tag. The Fosmid clones were pooled together, and DNA was extracted for paired-end library construction. Pooled Fosmid plasmid DNA was sheared into ~ 15 kb fragments by g-TUBE (Covaris). It generated insert only, vector with single-ends and vector with paired-ends. Then, these DNA fragments were end repaired and gel purified for ligation with the Ampicillin resistance gene tag. Although all fragments could be ligated to the Ampicillin resistance gene tag, only those containing the chloramphenicol resistant gene and ori V ligated to an Amp tag were screened out with double resistance to chloramphenicol and ampicillin after transformation. Finally, the vector was removed by I-SceI and the paired-end fragments with the Amp tag were sequenced on PacBio

    Journal: Plant Methods

    Article Title: High-throughput long paired-end sequencing of a Fosmid library by PacBio

    doi: 10.1186/s13007-019-0525-6

    Figure Lengend Snippet: The pipeline of Fosmid-size long paired-end library construction. The red area represents the vector, the blue area represents the large inserted genomic fragment, and the yellow area represents the Ampicillin resistance gene tag. The Fosmid clones were pooled together, and DNA was extracted for paired-end library construction. Pooled Fosmid plasmid DNA was sheared into ~ 15 kb fragments by g-TUBE (Covaris). It generated insert only, vector with single-ends and vector with paired-ends. Then, these DNA fragments were end repaired and gel purified for ligation with the Ampicillin resistance gene tag. Although all fragments could be ligated to the Ampicillin resistance gene tag, only those containing the chloramphenicol resistant gene and ori V ligated to an Amp tag were screened out with double resistance to chloramphenicol and ampicillin after transformation. Finally, the vector was removed by I-SceI and the paired-end fragments with the Amp tag were sequenced on PacBio

    Article Snippet: A total of 400 μg of pooled Fosmid DNA was sheared into fragments by g-TUBE (Covaris), with a mean size ranging from 6 to 20 kb.

    Techniques: Plasmid Preparation, Clone Assay, Generated, Purification, Ligation, Transformation Assay

    Dry pulverization method overview. Tissue is placed in Covaris tissueTUBES with adapters and attached glass vials (step 1). To keep samples both cold and brittle to facilitate pulverization, tissues are briefly submerged in liquid nitrogen (step 2) between successive rounds of pulverization (step 3). Following the pulverization of samples to a powder, the tissueTUBEs are inverted and tissue powder is collected into attached Covaris glass vials (step 4). Tissue powder is fixed, washed and stored as a pellet at −80°C.

    Journal: Epigenetics & Chromatin

    Article Title: Mapping genome-wide transcription factor binding sites in frozen tissues

    doi: 10.1186/1756-8935-6-30

    Figure Lengend Snippet: Dry pulverization method overview. Tissue is placed in Covaris tissueTUBES with adapters and attached glass vials (step 1). To keep samples both cold and brittle to facilitate pulverization, tissues are briefly submerged in liquid nitrogen (step 2) between successive rounds of pulverization (step 3). Following the pulverization of samples to a powder, the tissueTUBEs are inverted and tissue powder is collected into attached Covaris glass vials (step 4). Tissue powder is fixed, washed and stored as a pellet at −80°C.

    Article Snippet: Frozen tissue samples (≈200 to 800 mg each) were pulverized in Covaris tissue TUBEs (Covaris 520001) with attached adapters (Covaris 520017) and glass vials (Covaris 520010).

    Techniques: