Journal: PLoS ONE

Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

doi: 10.1371/journal.pone.0108877

Figure Lengend Snippet: Strains used in this study.

Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

Techniques:

Journal: PLoS ONE

Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

doi: 10.1371/journal.pone.0108877

Figure Lengend Snippet: A lane, 1, Archangium gephyra DSM 2261; lane 2, Corallococcus macrospores DSM 14697; lane 3, Corallococcus coralloides DSM 52499; lane 4, Myxococcus fulvus DSM 16525; lane 5, Myxococcus virescens DSM 2260; lane 6, Cystobacter minus DSM 14751; lane 7, Stigmatella erecta DSM 16858; lane 8, Byssovorax cruenta DSM 14533; B, Bands (1∼28) that were excised for sequence analysis are numbered.

Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

doi: 10.1371/journal.pone.0108877

Figure Lengend Snippet: Strains used in this study.

Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

Techniques:

Journal: PLoS ONE

Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

doi: 10.1371/journal.pone.0108877

Figure Lengend Snippet: A lane, 1, Archangium gephyra DSM 2261; lane 2, Corallococcus macrospores DSM 14697; lane 3, Corallococcus coralloides DSM 52499; lane 4, Myxococcus fulvus DSM 16525; lane 5, Myxococcus virescens DSM 2260; lane 6, Cystobacter minus DSM 14751; lane 7, Stigmatella erecta DSM 16858; lane 8, Byssovorax cruenta DSM 14533; B, Bands (1∼28) that were excised for sequence analysis are numbered.

Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

doi: 10.1371/journal.pone.0108877

Figure Lengend Snippet: Sequence similarity of excised DNA fragments.

Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

Techniques: Sequencing

Journal: PLoS ONE

Article Title: RNA-guided gene editing of the murine gammaherpesvirus 68 genome reduces infectious virus production

doi: 10.1371/journal.pone.0252313

Figure Lengend Snippet: Guide RNA sequences.

Article Snippet: These were digested and ligated into the pLentiCRISPR (Adgene#51760) [ ] vector that was digested with EcoRI and NdeI.

Techniques: Sequencing, Construct

Journal: PLoS ONE

Article Title: RNA-guided gene editing of the murine gammaherpesvirus 68 genome reduces infectious virus production

doi: 10.1371/journal.pone.0252313

Figure Lengend Snippet: A. Schematic representation of the approach to evaluate the effects of Cas9-gRNA-mediated gene editing in 293T cells upon co-transfection of MHV68 BACmid DNA with pLentiCRISPR plasmid(s). Created with BioRender.com . B. Infectious virus output analyzed 72 hrs post-transfection with the indicated gRNAs in single. Data is representative of two independent experiments performed in biological triplicate. C. Infectious virus output analyzed 72 hrs post-transfection with single or multiple gRNAs. Multiplex samples delivered 50g2, 73g2, OriLyt2g1 and g2. Ordinary one-way ANOVA followed by Dunnett’s multiple comparisons to NT2 was used to determine significance as indicated: ****, p<0.0001. Results are the compilation of two independent experiments, each with three biological replicates.

Article Snippet: These were digested and ligated into the pLentiCRISPR (Adgene#51760) [ ] vector that was digested with EcoRI and NdeI.

Techniques: Cotransfection, Plasmid Preparation, Transfection, Multiplex Assay