s wadsworthensis  (ATCC)


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    Structured Review

    ATCC s wadsworthensis
    IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. <t>wadsworthensis</t> , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p
    S Wadsworthensis, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s wadsworthensis/product/ATCC
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    s wadsworthensis - by Bioz Stars, 2022-09
    94/100 stars

    Images

    1) Product Images from "Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp."

    Article Title: Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp.

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2016.01706

    IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p
    Figure Legend Snippet: IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p

    Techniques Used: Positive Control, Negative Control

    2) Product Images from "Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp."

    Article Title: Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp.

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2016.01706

    IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p
    Figure Legend Snippet: IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p

    Techniques Used: Positive Control, Negative Control

    3) Product Images from " Application of Novel PCR-Based Methods for Detection, Quantitation, and Phylogenetic Characterization of Sutterella Species in Intestinal Biopsy Samples from Children with Autism and Gastrointestinal Disturbances"

    Article Title: Application of Novel PCR-Based Methods for Detection, Quantitation, and Phylogenetic Characterization of Sutterella Species in Intestinal Biopsy Samples from Children with Autism and Gastrointestinal Disturbances

    Journal: mBio

    doi: 10.1128/mBio.00261-11

    PCR-based detection of Sutterella 16S rRNA gene sequences (V6–V8 region and C4–V8 region) in biopsies from AUT-GI and Control-GI patients. (A) Agarose gel detection of 260-bp Sutterella products in ileal (4 biopsy samples/patient) and cecal (4 biopsy samples/patient) biopsy DNA using SuttFor and SuttRev primers (V6–V8 region) in conventional PCR assays. (B) Agarose gel detection of 715-bp Sutterella products in ileal and cecal biopsy DNA using pan-bacterial primer 515For and SuttRev primer (C4–V8) in conventional PCR assays. The negative control is PCR reagents with water substituted for DNA. The positive control is DNA isolated from cultured S. wadsworthensis (ATCC 51579).
    Figure Legend Snippet: PCR-based detection of Sutterella 16S rRNA gene sequences (V6–V8 region and C4–V8 region) in biopsies from AUT-GI and Control-GI patients. (A) Agarose gel detection of 260-bp Sutterella products in ileal (4 biopsy samples/patient) and cecal (4 biopsy samples/patient) biopsy DNA using SuttFor and SuttRev primers (V6–V8 region) in conventional PCR assays. (B) Agarose gel detection of 715-bp Sutterella products in ileal and cecal biopsy DNA using pan-bacterial primer 515For and SuttRev primer (C4–V8) in conventional PCR assays. The negative control is PCR reagents with water substituted for DNA. The positive control is DNA isolated from cultured S. wadsworthensis (ATCC 51579).

    Techniques Used: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Negative Control, Positive Control, Isolation, Cell Culture

    Western immunoblot analysis of AUT-GI and Control-GI patients’ plasma antibody immunoreactivity against S. wadsworthensis antigens. (A) Patients’ plasma IgG antibody immunoreactivity against S. wadsworthensis antigens. (B) Patients’ IgM antibody immunoreactivity against S. wadsworthensis antigens. 2°, secondary antibody control.
    Figure Legend Snippet: Western immunoblot analysis of AUT-GI and Control-GI patients’ plasma antibody immunoreactivity against S. wadsworthensis antigens. (A) Patients’ plasma IgG antibody immunoreactivity against S. wadsworthensis antigens. (B) Patients’ IgM antibody immunoreactivity against S. wadsworthensis antigens. 2°, secondary antibody control.

    Techniques Used: Western Blot

    4) Product Images from "Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp."

    Article Title: Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp.

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2016.01706

    IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p
    Figure Legend Snippet: IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p

    Techniques Used: Positive Control, Negative Control

    5) Product Images from " Application of Novel PCR-Based Methods for Detection, Quantitation, and Phylogenetic Characterization of Sutterella Species in Intestinal Biopsy Samples from Children with Autism and Gastrointestinal Disturbances"

    Article Title: Application of Novel PCR-Based Methods for Detection, Quantitation, and Phylogenetic Characterization of Sutterella Species in Intestinal Biopsy Samples from Children with Autism and Gastrointestinal Disturbances

    Journal: mBio

    doi: 10.1128/mBio.00261-11

    PCR-based detection of Sutterella 16S rRNA gene sequences (V6–V8 region and C4–V8 region) in biopsies from AUT-GI and Control-GI patients. (A) Agarose gel detection of 260-bp Sutterella products in ileal (4 biopsy samples/patient) and cecal (4 biopsy samples/patient) biopsy DNA using SuttFor and SuttRev primers (V6–V8 region) in conventional PCR assays. (B) Agarose gel detection of 715-bp Sutterella products in ileal and cecal biopsy DNA using pan-bacterial primer 515For and SuttRev primer (C4–V8) in conventional PCR assays. The negative control is PCR reagents with water substituted for DNA. The positive control is DNA isolated from cultured S. wadsworthensis (ATCC 51579).
    Figure Legend Snippet: PCR-based detection of Sutterella 16S rRNA gene sequences (V6–V8 region and C4–V8 region) in biopsies from AUT-GI and Control-GI patients. (A) Agarose gel detection of 260-bp Sutterella products in ileal (4 biopsy samples/patient) and cecal (4 biopsy samples/patient) biopsy DNA using SuttFor and SuttRev primers (V6–V8 region) in conventional PCR assays. (B) Agarose gel detection of 715-bp Sutterella products in ileal and cecal biopsy DNA using pan-bacterial primer 515For and SuttRev primer (C4–V8) in conventional PCR assays. The negative control is PCR reagents with water substituted for DNA. The positive control is DNA isolated from cultured S. wadsworthensis (ATCC 51579).

    Techniques Used: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Negative Control, Positive Control, Isolation, Cell Culture

    Western immunoblot analysis of AUT-GI and Control-GI patients’ plasma antibody immunoreactivity against S. wadsworthensis antigens. (A) Patients’ plasma IgG antibody immunoreactivity against S. wadsworthensis antigens. (B) Patients’ IgM antibody immunoreactivity against S. wadsworthensis antigens. 2°, secondary antibody control.
    Figure Legend Snippet: Western immunoblot analysis of AUT-GI and Control-GI patients’ plasma antibody immunoreactivity against S. wadsworthensis antigens. (A) Patients’ plasma IgG antibody immunoreactivity against S. wadsworthensis antigens. (B) Patients’ IgM antibody immunoreactivity against S. wadsworthensis antigens. 2°, secondary antibody control.

    Techniques Used: Western Blot

    6) Product Images from " Application of Novel PCR-Based Methods for Detection, Quantitation, and Phylogenetic Characterization of Sutterella Species in Intestinal Biopsy Samples from Children with Autism and Gastrointestinal Disturbances"

    Article Title: Application of Novel PCR-Based Methods for Detection, Quantitation, and Phylogenetic Characterization of Sutterella Species in Intestinal Biopsy Samples from Children with Autism and Gastrointestinal Disturbances

    Journal: mBio

    doi: 10.1128/mBio.00261-11

    PCR-based detection of Sutterella 16S rRNA gene sequences (V6–V8 region and C4–V8 region) in biopsies from AUT-GI and Control-GI patients. (A) Agarose gel detection of 260-bp Sutterella products in ileal (4 biopsy samples/patient) and cecal (4 biopsy samples/patient) biopsy DNA using SuttFor and SuttRev primers (V6–V8 region) in conventional PCR assays. (B) Agarose gel detection of 715-bp Sutterella products in ileal and cecal biopsy DNA using pan-bacterial primer 515For and SuttRev primer (C4–V8) in conventional PCR assays. The negative control is PCR reagents with water substituted for DNA. The positive control is DNA isolated from cultured S. wadsworthensis (ATCC 51579).
    Figure Legend Snippet: PCR-based detection of Sutterella 16S rRNA gene sequences (V6–V8 region and C4–V8 region) in biopsies from AUT-GI and Control-GI patients. (A) Agarose gel detection of 260-bp Sutterella products in ileal (4 biopsy samples/patient) and cecal (4 biopsy samples/patient) biopsy DNA using SuttFor and SuttRev primers (V6–V8 region) in conventional PCR assays. (B) Agarose gel detection of 715-bp Sutterella products in ileal and cecal biopsy DNA using pan-bacterial primer 515For and SuttRev primer (C4–V8) in conventional PCR assays. The negative control is PCR reagents with water substituted for DNA. The positive control is DNA isolated from cultured S. wadsworthensis (ATCC 51579).

    Techniques Used: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Negative Control, Positive Control, Isolation, Cell Culture

    Western immunoblot analysis of AUT-GI and Control-GI patients’ plasma antibody immunoreactivity against S. wadsworthensis antigens. (A) Patients’ plasma IgG antibody immunoreactivity against S. wadsworthensis antigens. (B) Patients’ IgM antibody immunoreactivity against S. wadsworthensis antigens. 2°, secondary antibody control.
    Figure Legend Snippet: Western immunoblot analysis of AUT-GI and Control-GI patients’ plasma antibody immunoreactivity against S. wadsworthensis antigens. (A) Patients’ plasma IgG antibody immunoreactivity against S. wadsworthensis antigens. (B) Patients’ IgM antibody immunoreactivity against S. wadsworthensis antigens. 2°, secondary antibody control.

    Techniques Used: Western Blot

    7) Product Images from "Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp."

    Article Title: Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp.

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2016.01706

    IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p
    Figure Legend Snippet: IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p

    Techniques Used: Positive Control, Negative Control

    8) Product Images from "Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp."

    Article Title: Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp.

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2016.01706

    IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p
    Figure Legend Snippet: IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p

    Techniques Used: Positive Control, Negative Control

    9) Product Images from "Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp."

    Article Title: Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp.

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2016.01706

    IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p
    Figure Legend Snippet: IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p

    Techniques Used: Positive Control, Negative Control

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    ATCC s wadsworthensis
    IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. <t>wadsworthensis</t> , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p
    S Wadsworthensis, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/s wadsworthensis/product/ATCC
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    s wadsworthensis - by Bioz Stars, 2022-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p

    Journal: Frontiers in Microbiology

    Article Title: Mucosal Prevalence and Interactions with the Epithelium Indicate Commensalism of Sutterella spp.

    doi: 10.3389/fmicb.2016.01706

    Figure Lengend Snippet: IL-8 production in HT-29 cells induced by bacterial cell suspensions and LPS preparations. (A) IL-8 response in HT-29 cells by S. wadsworthensis , S. parvirubra , S. stercoricanis , B. fragilis (positive control), and E. coli (negative control) with 1:10, 1:100, and 1:1,000 dilutions from OD 600 nm 0.25 adjusted cell suspensions. The 1:10 dilution is not shown for E. coli due to excessive toxicity to the HT-29 cells. (B) IL-8 production induced by 1:1,000 and 1:10,000 dilutions of LPS preparations from E. coli and Sutterella spp. LPS preparations were made from the OD 600 nm 0.25 normalized cell suspensions and the dilutions were thereof. Results from the representative experiment are shown as means and standard deviations of three technical replicates (parallel wells). The culture medium was used as a background control in both assays. An asterisk indicates a significant ( ∗ p

    Article Snippet: Sutterella parvirubra Adheres Best to Differentiated Enterocytes and Can Displace S. wadsworthensis To compare the adhesion capability of Sutterella spp. to IECs, we used the Caco-2 and HT-29 cell lines as models of enterocytes.

    Techniques: Positive Control, Negative Control

    PCR-based detection of Sutterella 16S rRNA gene sequences (V6–V8 region and C4–V8 region) in biopsies from AUT-GI and Control-GI patients. (A) Agarose gel detection of 260-bp Sutterella products in ileal (4 biopsy samples/patient) and cecal (4 biopsy samples/patient) biopsy DNA using SuttFor and SuttRev primers (V6–V8 region) in conventional PCR assays. (B) Agarose gel detection of 715-bp Sutterella products in ileal and cecal biopsy DNA using pan-bacterial primer 515For and SuttRev primer (C4–V8) in conventional PCR assays. The negative control is PCR reagents with water substituted for DNA. The positive control is DNA isolated from cultured S. wadsworthensis (ATCC 51579).

    Journal: mBio

    Article Title: Application of Novel PCR-Based Methods for Detection, Quantitation, and Phylogenetic Characterization of Sutterella Species in Intestinal Biopsy Samples from Children with Autism and Gastrointestinal Disturbances

    doi: 10.1128/mBio.00261-11

    Figure Lengend Snippet: PCR-based detection of Sutterella 16S rRNA gene sequences (V6–V8 region and C4–V8 region) in biopsies from AUT-GI and Control-GI patients. (A) Agarose gel detection of 260-bp Sutterella products in ileal (4 biopsy samples/patient) and cecal (4 biopsy samples/patient) biopsy DNA using SuttFor and SuttRev primers (V6–V8 region) in conventional PCR assays. (B) Agarose gel detection of 715-bp Sutterella products in ileal and cecal biopsy DNA using pan-bacterial primer 515For and SuttRev primer (C4–V8) in conventional PCR assays. The negative control is PCR reagents with water substituted for DNA. The positive control is DNA isolated from cultured S. wadsworthensis (ATCC 51579).

    Article Snippet: Conventional PCR for detection of Sutterella was carried out in 25-µl reaction mixtures consisting of 25 ng of biopsy DNA or 25 pg of genomic DNA from cultured S. wadsworthensis (ATCC 51579; positive control), 300 nM each of the SuttFor and SuttRev primers (for V6–V8 amplification) or 515For and SuttRev (for C4–V8 amplification), 2 µl deoxynucleoside triphosphate (dNTP) mix (10 mM; Applied Biosystems, Foster City, CA), 2.5 µl of 10× PCR buffer (Qiagen, Valencia, CA), 5 U of HotStarTaq DNA polymerase (Qiagen), and 5 µl Q-solution (Qiagen).

    Techniques: Polymerase Chain Reaction, Agarose Gel Electrophoresis, Negative Control, Positive Control, Isolation, Cell Culture

    Western immunoblot analysis of AUT-GI and Control-GI patients’ plasma antibody immunoreactivity against S. wadsworthensis antigens. (A) Patients’ plasma IgG antibody immunoreactivity against S. wadsworthensis antigens. (B) Patients’ IgM antibody immunoreactivity against S. wadsworthensis antigens. 2°, secondary antibody control.

    Journal: mBio

    Article Title: Application of Novel PCR-Based Methods for Detection, Quantitation, and Phylogenetic Characterization of Sutterella Species in Intestinal Biopsy Samples from Children with Autism and Gastrointestinal Disturbances

    doi: 10.1128/mBio.00261-11

    Figure Lengend Snippet: Western immunoblot analysis of AUT-GI and Control-GI patients’ plasma antibody immunoreactivity against S. wadsworthensis antigens. (A) Patients’ plasma IgG antibody immunoreactivity against S. wadsworthensis antigens. (B) Patients’ IgM antibody immunoreactivity against S. wadsworthensis antigens. 2°, secondary antibody control.

    Article Snippet: Conventional PCR for detection of Sutterella was carried out in 25-µl reaction mixtures consisting of 25 ng of biopsy DNA or 25 pg of genomic DNA from cultured S. wadsworthensis (ATCC 51579; positive control), 300 nM each of the SuttFor and SuttRev primers (for V6–V8 amplification) or 515For and SuttRev (for C4–V8 amplification), 2 µl deoxynucleoside triphosphate (dNTP) mix (10 mM; Applied Biosystems, Foster City, CA), 2.5 µl of 10× PCR buffer (Qiagen, Valencia, CA), 5 U of HotStarTaq DNA polymerase (Qiagen), and 5 µl Q-solution (Qiagen).

    Techniques: Western Blot