sutterella wadsworthensis 3 1 45b  (ATCC)

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 1 Clusterin expression in response to a DDC diet and bile acids. (A) Representative immunohistochemical images of clusterin expression in liver tis sues of C57BL/6 mice fed a DDC diet for 10 (n = 4) and 20 days (n = 4) compared with controls (n = 4). Data in the bar graph are means ± SEM. ***p < 0.001. Scale bars indicate 100 μm. (B) Representative western blot analysis of clusterin expression in liver tissues of mice fed a DDC diet. Data in the bar graph are means ± SEM. *p < 0.05, **p < 0.01. (C) Representative western blot analysis of clusterin expression in lysates of primary HCs treated with TCA, CA, and CDCA. (D) Immunohistochemical analysis of clusterin in liver sections from patients with PBC compared with control liver tissues. Scale bars indicate 100 μm. p: portal tract

Article Snippet: Immunohistochemical staining was performed using an anti-clusterin antibody (Santa Cruz Biotechnology) and an automatic staining device (Benchmark XT; Ventana Medical Systems, Mountain View, CA, USA) in accordance with the manufacturers’ protocols.

Techniques: Expressing, Immunohistochemical staining, Western Blot, Control

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 2 Clusterin deficiency promotes fibrosis upon DDC diet-induced liver injury. (A, B) Representative images of hematoxylin and eosin (A) and Sirius red (B) staining in WT and clusterin-KO mice fed a DDC diet (n = 4). **p < 0.01, ***p < 0.001. (C, D) Immunohistochemical analysis of collagen (C) and αSMA (D) expression in WT and clusterin-KO mice fed a DDC diet. Data in the bar graph are means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars indicate 100 μm

Article Snippet: Immunohistochemical staining was performed using an anti-clusterin antibody (Santa Cruz Biotechnology) and an automatic staining device (Benchmark XT; Ventana Medical Systems, Mountain View, CA, USA) in accordance with the manufacturers’ protocols.

Techniques: Staining, Immunohistochemical staining, Expressing

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 3 Effects of clusterin deficiency on DDC diet-induced liver injury. (A, B) Representative real-time RT-PCR (A) and western blot (B) analyses of type I collagen and αSMA expression in WT and clusterin-KO mice fed a DDC diet for 20 days. *p < 0.05, **p < 0.01. (C, D) ELISAs of ALT (C) and AST (D) levels in serum of WT and clusterin-KO mice fed a DDC diet for 10 and 20 days. (E, F) ELISAs of IL1β (E) and IL18 (F) levels in serum of WT and clusterin-KO mice fed a DDC diet for 10 and 20 days. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Immunohistochemical staining was performed using an anti-clusterin antibody (Santa Cruz Biotechnology) and an automatic staining device (Benchmark XT; Ventana Medical Systems, Mountain View, CA, USA) in accordance with the manufacturers’ protocols.

Techniques: Quantitative RT-PCR, Western Blot, Expressing

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 4 NLRP3 inflammasome activation in clusterin-KO mice fed a DDC diet. (A, B) Immunohistochemical analysis of NLRP3 (A) and IL1β (B) expression in WT and clusterin-KO mice fed a DDC diet for 20 days. Data in the bar graph are means ± SEM. ***p < 0.001. Scale bars indicate 100 μm. (C) Representative western blot analysis of NLRP3 inflammasome markers in WT and clusterin-KO mice fed a DDC diet for 20 days. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Immunohistochemical staining was performed using an anti-clusterin antibody (Santa Cruz Biotechnology) and an automatic staining device (Benchmark XT; Ventana Medical Systems, Mountain View, CA, USA) in accordance with the manufacturers’ protocols.

Techniques: Activation Assay, Immunohistochemical staining, Expressing, Western Blot

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 5 ER stress response in clusterin-KO mice fed a DDC diet. (A, B) Immunohistochemical analysis of ATF6 (A) and CHOP (B) expression in WT and clusterin-KO mice fed a DDC diet for 20 days. Data in the bar graph are means ± SEM. **p < 0.01, ***p < 0.001. Scale bars indicate 100 μm. (C) Representative western blot analysis of ER stress markers in WT and clusterin-KO mice fed a DDC diet for 20 days. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Immunohistochemical staining was performed using an anti-clusterin antibody (Santa Cruz Biotechnology) and an automatic staining device (Benchmark XT; Ventana Medical Systems, Mountain View, CA, USA) in accordance with the manufacturers’ protocols.

Techniques: Immunohistochemical staining, Expressing, Western Blot

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 6 ER stress enhances NLRP3 inflammasome activation in clusterin-KO primary HCs and KCs (A, B) Representative real-time RT-PCR analysis of NLRP3, IL1β, TNFα, and IL6 mRNA levels in clusterin-KO primary KCs (A) and HCs (B) treated with Tg (1 μM, 24 h). *p < 0.05, **p < 0.01, ***p < 0.001. (C) ELISA of IL1β levels in clusterin-KO primary HCs and KCs treated with Tg (1 μM, 24 h). *p < 0.05, ***p < 0.001. (D) Representative western blot analysis of NLRP3 and IL1β protein expression in lysates of clusterin-KO primary HCs and KCs treated with Tg

Article Snippet: Immunohistochemical staining was performed using an anti-clusterin antibody (Santa Cruz Biotechnology) and an automatic staining device (Benchmark XT; Ventana Medical Systems, Mountain View, CA, USA) in accordance with the manufacturers’ protocols.

Techniques: Activation Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 7 Clusterin attenuates ER stress-induced NLRP3 inflammasome activation. (A, B) Real-time RT-PCR analysis of the effects of clusterin on Tg-induced (1 µM, 24 h) mRNA expression of NLRP3, IL1β, and TNFα in primary HCs (A) and KCs (B). *p < 0.05, **p < 0.01, ***p < 0.001. (C, D) Western blot analysis of the effects of clusterin on Tg-induced protein expression of NLRP3 and IL1β in lysates of primary HCs (C) and KCs (D). (E, F) Real-time RT-PCR (E) and western blot (F) analyses of the effects of clusterin on Tg-induced NLRP3 and IL1β expression in THP-1 cells. *p < 0.05, **p < 0.01

Article Snippet: Immunohistochemical staining was performed using an anti-clusterin antibody (Santa Cruz Biotechnology) and an automatic staining device (Benchmark XT; Ventana Medical Systems, Mountain View, CA, USA) in accordance with the manufacturers’ protocols.

Techniques: Activation Assay, Quantitative RT-PCR, Expressing, Western Blot

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 1 Clusterin expression in response to a DDC diet and bile acids. (A) Representative immunohistochemical images of clusterin expression in liver tis sues of C57BL/6 mice fed a DDC diet for 10 (n = 4) and 20 days (n = 4) compared with controls (n = 4). Data in the bar graph are means ± SEM. ***p < 0.001. Scale bars indicate 100 μm. (B) Representative western blot analysis of clusterin expression in liver tissues of mice fed a DDC diet. Data in the bar graph are means ± SEM. *p < 0.05, **p < 0.01. (C) Representative western blot analysis of clusterin expression in lysates of primary HCs treated with TCA, CA, and CDCA. (D) Immunohistochemical analysis of clusterin in liver sections from patients with PBC compared with control liver tissues. Scale bars indicate 100 μm. p: portal tract

Article Snippet: Antibodies against clusterin (SC6420), CHOP (SC7351), ATF6 (SC166659), CTGF (SC365970) and ASC (SC514414) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Expressing, Immunohistochemical staining, Western Blot, Control

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 2 Clusterin deficiency promotes fibrosis upon DDC diet-induced liver injury. (A, B) Representative images of hematoxylin and eosin (A) and Sirius red (B) staining in WT and clusterin-KO mice fed a DDC diet (n = 4). **p < 0.01, ***p < 0.001. (C, D) Immunohistochemical analysis of collagen (C) and αSMA (D) expression in WT and clusterin-KO mice fed a DDC diet. Data in the bar graph are means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. Scale bars indicate 100 μm

Article Snippet: Antibodies against clusterin (SC6420), CHOP (SC7351), ATF6 (SC166659), CTGF (SC365970) and ASC (SC514414) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Staining, Immunohistochemical staining, Expressing

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 3 Effects of clusterin deficiency on DDC diet-induced liver injury. (A, B) Representative real-time RT-PCR (A) and western blot (B) analyses of type I collagen and αSMA expression in WT and clusterin-KO mice fed a DDC diet for 20 days. *p < 0.05, **p < 0.01. (C, D) ELISAs of ALT (C) and AST (D) levels in serum of WT and clusterin-KO mice fed a DDC diet for 10 and 20 days. (E, F) ELISAs of IL1β (E) and IL18 (F) levels in serum of WT and clusterin-KO mice fed a DDC diet for 10 and 20 days. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Antibodies against clusterin (SC6420), CHOP (SC7351), ATF6 (SC166659), CTGF (SC365970) and ASC (SC514414) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Quantitative RT-PCR, Western Blot, Expressing

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 4 NLRP3 inflammasome activation in clusterin-KO mice fed a DDC diet. (A, B) Immunohistochemical analysis of NLRP3 (A) and IL1β (B) expression in WT and clusterin-KO mice fed a DDC diet for 20 days. Data in the bar graph are means ± SEM. ***p < 0.001. Scale bars indicate 100 μm. (C) Representative western blot analysis of NLRP3 inflammasome markers in WT and clusterin-KO mice fed a DDC diet for 20 days. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Antibodies against clusterin (SC6420), CHOP (SC7351), ATF6 (SC166659), CTGF (SC365970) and ASC (SC514414) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Activation Assay, Immunohistochemical staining, Expressing, Western Blot

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 5 ER stress response in clusterin-KO mice fed a DDC diet. (A, B) Immunohistochemical analysis of ATF6 (A) and CHOP (B) expression in WT and clusterin-KO mice fed a DDC diet for 20 days. Data in the bar graph are means ± SEM. **p < 0.01, ***p < 0.001. Scale bars indicate 100 μm. (C) Representative western blot analysis of ER stress markers in WT and clusterin-KO mice fed a DDC diet for 20 days. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Antibodies against clusterin (SC6420), CHOP (SC7351), ATF6 (SC166659), CTGF (SC365970) and ASC (SC514414) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Immunohistochemical staining, Expressing, Western Blot

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 6 ER stress enhances NLRP3 inflammasome activation in clusterin-KO primary HCs and KCs (A, B) Representative real-time RT-PCR analysis of NLRP3, IL1β, TNFα, and IL6 mRNA levels in clusterin-KO primary KCs (A) and HCs (B) treated with Tg (1 μM, 24 h). *p < 0.05, **p < 0.01, ***p < 0.001. (C) ELISA of IL1β levels in clusterin-KO primary HCs and KCs treated with Tg (1 μM, 24 h). *p < 0.05, ***p < 0.001. (D) Representative western blot analysis of NLRP3 and IL1β protein expression in lysates of clusterin-KO primary HCs and KCs treated with Tg

Article Snippet: Antibodies against clusterin (SC6420), CHOP (SC7351), ATF6 (SC166659), CTGF (SC365970) and ASC (SC514414) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Activation Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Western Blot, Expressing

Journal: Cell & bioscience

Article Title: Clusterin deficiency exacerbates cholestatic liver disease through ER stress and NLRP3 inflammasome activation.

doi: 10.1186/s13578-025-01376-z

Figure Lengend Snippet: Fig. 7 Clusterin attenuates ER stress-induced NLRP3 inflammasome activation. (A, B) Real-time RT-PCR analysis of the effects of clusterin on Tg-induced (1 µM, 24 h) mRNA expression of NLRP3, IL1β, and TNFα in primary HCs (A) and KCs (B). *p < 0.05, **p < 0.01, ***p < 0.001. (C, D) Western blot analysis of the effects of clusterin on Tg-induced protein expression of NLRP3 and IL1β in lysates of primary HCs (C) and KCs (D). (E, F) Real-time RT-PCR (E) and western blot (F) analyses of the effects of clusterin on Tg-induced NLRP3 and IL1β expression in THP-1 cells. *p < 0.05, **p < 0.01

Article Snippet: Antibodies against clusterin (SC6420), CHOP (SC7351), ATF6 (SC166659), CTGF (SC365970) and ASC (SC514414) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).

Techniques: Activation Assay, Quantitative RT-PCR, Expressing, Western Blot

Journal: Frontiers in Cell and Developmental Biology

Article Title: Glioma-associated oncogene homolog 1 in breast invasive carcinoma: a comprehensive bioinformatic analysis and experimental validation

doi: 10.3389/fcell.2024.1478478

Figure Lengend Snippet: The primers used in the experiments.

Article Snippet: Following dewaxing, rehydration, and antigen retrieval, the sections were incubated overnight at 4°C with anti-GLI1 antibody (SC-166907, 1:500, Santa Cruz, CA, United States).

Techniques: Sequencing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Glioma-associated oncogene homolog 1 in breast invasive carcinoma: a comprehensive bioinformatic analysis and experimental validation

doi: 10.3389/fcell.2024.1478478

Figure Lengend Snippet: Expressions of GLI1 in pan-cancers. Expression levels of GLI1 in various cancers or tumor tissues and adjacent non-tumor tissues analyzed by TIMER2.0 (A) , GEPIA2 (B) and the R package (C) based on TCGA and/or GTEx databases. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Following dewaxing, rehydration, and antigen retrieval, the sections were incubated overnight at 4°C with anti-GLI1 antibody (SC-166907, 1:500, Santa Cruz, CA, United States).

Techniques: Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Glioma-associated oncogene homolog 1 in breast invasive carcinoma: a comprehensive bioinformatic analysis and experimental validation

doi: 10.3389/fcell.2024.1478478

Figure Lengend Snippet: Expression pattern of GLI1 in BRCA. (A) , Differential expression of GLI1 between BRCA tissue and normal tissue samples in TCGA and GTEx; (B) , The qPCR results; (C, D) , Representative images and quantification of immunohistochemical staining for GLI1 in BRCA tissue and normal tissue for patient 1 and 2.

Article Snippet: Following dewaxing, rehydration, and antigen retrieval, the sections were incubated overnight at 4°C with anti-GLI1 antibody (SC-166907, 1:500, Santa Cruz, CA, United States).

Techniques: Expressing, Quantitative Proteomics, Immunohistochemical staining, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: Glioma-associated oncogene homolog 1 in breast invasive carcinoma: a comprehensive bioinformatic analysis and experimental validation

doi: 10.3389/fcell.2024.1478478

Figure Lengend Snippet: Prognostic value of GLI1 in BRCA. (A–D) , Survival curves showing the differences on overall survival (A) , disease-specific survival (B) , progression-free interval (C) and disease-free interval (D) between high and low GLI1 expression; (E–J) , Correlation box diagram of GLI1 expression difference and clinical factors; (K) , Heat map of correlation between GLI1 expression difference and clinical factors.

Article Snippet: Following dewaxing, rehydration, and antigen retrieval, the sections were incubated overnight at 4°C with anti-GLI1 antibody (SC-166907, 1:500, Santa Cruz, CA, United States).

Techniques: Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Glioma-associated oncogene homolog 1 in breast invasive carcinoma: a comprehensive bioinformatic analysis and experimental validation

doi: 10.3389/fcell.2024.1478478

Figure Lengend Snippet: Associations of GLI1 with immune infiltration in BRCA. (A) , Cumulative survival of BRCA patients with high and low infiltrating levels of six immune cells in TIMER2.0; (B) , Correlations between GLI1 expression and infiltrating levels of six immune cells in TIMER2.0; (C, D) , Correlations of GLI1 expression with stromal (C) and immune (D) scores; (E–O) , Correlations between GLI1 expression and infiltrating levels of the immune cells by CIBERSORT; (P) Correlations between GLI1 expression and infiltrating levels of the immune cells by xCELL.

Article Snippet: Following dewaxing, rehydration, and antigen retrieval, the sections were incubated overnight at 4°C with anti-GLI1 antibody (SC-166907, 1:500, Santa Cruz, CA, United States).

Techniques: Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Glioma-associated oncogene homolog 1 in breast invasive carcinoma: a comprehensive bioinformatic analysis and experimental validation

doi: 10.3389/fcell.2024.1478478

Figure Lengend Snippet: Associations of GLI1 expression with immune status-related genes and immunotherapy. Co-expression of GLI1 expression with chemokines (A) , receptors (B) , MHC (C) , immunoinhibitors (D) , immunostimulators (E) , immune checkpoint suppressor genes (F) and immune checkpoint-promoting genes (G) . Correlation analysis of gene expression and combined application of immunotherapy CTLA-4 and PD-1 (H–K) * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Following dewaxing, rehydration, and antigen retrieval, the sections were incubated overnight at 4°C with anti-GLI1 antibody (SC-166907, 1:500, Santa Cruz, CA, United States).

Techniques: Expressing, Gene Expression

Journal: Frontiers in Cell and Developmental Biology

Article Title: Glioma-associated oncogene homolog 1 in breast invasive carcinoma: a comprehensive bioinformatic analysis and experimental validation

doi: 10.3389/fcell.2024.1478478

Figure Lengend Snippet: Gene set difference analysis and enrichment analysis. Heat map of high and low differential expression of GLI1 and related genes (A) . GLI1 expression is significantly associated with KEGG pathways (B) and gene ontology terms (C) .

Article Snippet: Following dewaxing, rehydration, and antigen retrieval, the sections were incubated overnight at 4°C with anti-GLI1 antibody (SC-166907, 1:500, Santa Cruz, CA, United States).

Techniques: Quantitative Proteomics, Expressing

Journal: Frontiers in Cell and Developmental Biology

Article Title: Glioma-associated oncogene homolog 1 in breast invasive carcinoma: a comprehensive bioinformatic analysis and experimental validation

doi: 10.3389/fcell.2024.1478478

Figure Lengend Snippet: Illustrates the expression of GLI1 in BRCA tumors under the influence of various drugs. (A) AGI-5198, (B) GSK1904529A, (C) Rapamycin, (D) Axitinib, (E) Bicalutamide, (F) Camptothecin, (G) Cisplatin, (H) Cytarabine, (I) Docetaxel, (J) Embelin, (K) Gemcitabine, (L) Imatinib, and (M) Lenalidomide.

Article Snippet: Following dewaxing, rehydration, and antigen retrieval, the sections were incubated overnight at 4°C with anti-GLI1 antibody (SC-166907, 1:500, Santa Cruz, CA, United States).

Techniques: Expressing