Journal: PLoS ONE

Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

doi: 10.1371/journal.pone.0108877

Figure Lengend Snippet: Strains used in this study.

Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

Techniques:

Journal: PLoS ONE

Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

doi: 10.1371/journal.pone.0108877

Figure Lengend Snippet: A lane, 1, Archangium gephyra DSM 2261; lane 2, Corallococcus macrospores DSM 14697; lane 3, Corallococcus coralloides DSM 52499; lane 4, Myxococcus fulvus DSM 16525; lane 5, Myxococcus virescens DSM 2260; lane 6, Cystobacter minus DSM 14751; lane 7, Stigmatella erecta DSM 16858; lane 8, Byssovorax cruenta DSM 14533; B, Bands (1∼28) that were excised for sequence analysis are numbered.

Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

doi: 10.1371/journal.pone.0108877

Figure Lengend Snippet: Strains used in this study.

Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

Techniques:

Journal: PLoS ONE

Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

doi: 10.1371/journal.pone.0108877

Figure Lengend Snippet: A lane, 1, Archangium gephyra DSM 2261; lane 2, Corallococcus macrospores DSM 14697; lane 3, Corallococcus coralloides DSM 52499; lane 4, Myxococcus fulvus DSM 16525; lane 5, Myxococcus virescens DSM 2260; lane 6, Cystobacter minus DSM 14751; lane 7, Stigmatella erecta DSM 16858; lane 8, Byssovorax cruenta DSM 14533; B, Bands (1∼28) that were excised for sequence analysis are numbered.

Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

Techniques: Sequencing

Journal: PLoS ONE

Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

doi: 10.1371/journal.pone.0108877

Figure Lengend Snippet: Sequence similarity of excised DNA fragments.

Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

Techniques: Sequencing

Journal: Nutrients

Article Title: Anti-Aging and Neuroprotective Properties of Grifola frondosa and Hericium erinaceus Extracts

doi: 10.3390/nu14204368

Figure Lengend Snippet: Fungal extracts reduce α-synuclein toxicity. ( A ) CLS of wt[empty] and wt[αSyn] cells grown in minimal medium containing 2% glucose in the absence or presence of 0.5% extract of G. frondosa or H. erinaceus . ( B ) Aggresome content (assayed by the PROTEOSTAT Aggresome kit) of wt[empty] and wt[αSyn] cells grown for 24 h in the absence or presence of 0.5% extract of G. frondosa or H. erinaceus . ( C ) ROS content (assayed by DHE staining) of wt[empty] and wt[αSyn] cells grown for 24 h in the absence or presence of 0.5% extract of G. frondosa or H. erinaceus . ( D ) Mitochondrial membrane potential (assayed by DiOC 6 staining) of wt[empty] and wt[αSyn] cells grown for 24 h in the absence or presence of 0.5% extract of G. frondosa or H. erinaceus . ( B – D ) Mean ± standard deviations are shown. * p < 0.05 relative to control wt[empty] cells , ° p < 0.05 relative to control wt[αSyn] cells. ( E ) wt[αSyn] cells were grown in minimal medium containing 2% glucose in the absence or presence of 0.5% extract of G. frondosa or H. erinaceus for 24 h. Cells in exponential phase of growth were treated with the indicated concentrations of H 2 O 2 for 4 h and cell viability was assayed with propidium iodide staining by cytofluorimetric analysis. Mean ± standard deviations are shown. * p < 0.05 relative to the same concentration of H 2 O 2 in cells. ( F ) (A) CLS of wt[αSyn] cells grown in minimal medium containing 2% glucose in the absence or presence of ergothioneine (ET) at the following concentrations: a = 0.0005%, b = 0.00075%.

Article Snippet: Anti-α-Synuclein XP ® Rabbit (#51510 Cell Signaling Technology, Beverly, MA, USA) antibody was used at 1:1,000 dilution.

Techniques: Staining, Concentration Assay

Journal: Nutrients

Article Title: Anti-Aging and Neuroprotective Properties of Grifola frondosa and Hericium erinaceus Extracts

doi: 10.3390/nu14204368

Figure Lengend Snippet: Fungal extracts induce α-synuclein delocalization. ( A ) Western analysis using anti-α-synuclein antibody on total extracts from wt[αSyn] cells after 24 h treatment with 0.5% fungal extract. Pgk1 was used as loading control. ( B ) Western analysis using anti-GFP antibody on total extracts from wt[αSyn][Atg8-GFP] cells treated with 0.5% fungal extract. PonceauS staining was used as loading control. ( C ) Immunofluorescence showing localization of α-synuclein in cells untreated or treated for 1 day with 0.5% fungal extracts. ( D ) Western analysis using anti-α-synuclein antibody on cytoplasmic and membrane fractions isolated from wt[αSyn] cells after 24 h treatment with 0.5% fungi extract. Pgk1 was used as cytoplasmic marker, Pma1 as membrane marker. ( E , F ) wt[αSyn] cells were grown in exponential phase in the presence or absence of 0.5% extract of G. frondosa or H. erinaceus and stained for 15 min with FM4-64, as reported in materials and methods. Cells with or without FM4-64 staining at the vacuolar membrane were counted at the microscope, mean ± standard deviations are shown. * p < 0.05 relative to control condition (no extract).

Article Snippet: Anti-α-Synuclein XP ® Rabbit (#51510 Cell Signaling Technology, Beverly, MA, USA) antibody was used at 1:1,000 dilution.

Techniques: Western Blot, Staining, Immunofluorescence, Isolation, Marker, Microscopy

Journal: Nutrients

Article Title: Anti-Aging and Neuroprotective Properties of Grifola frondosa and Hericium erinaceus Extracts

doi: 10.3390/nu14204368

Figure Lengend Snippet: Fungal extract effects on α-syn aggregation process. ( A ) ThT fluorescence intensity of α-syn samples aggregated in the absence (α-syn) or in the presence of G. frondosa ( a -syn-G.f.) or H. erinaceus (a-syn-H.e.) extract at α-syn:extract mass ratio 1:1 (1x) recorded at different times of aggregation (0 h, 24 h, 48 h and 72 h) or ( B ) in continuous for the first 15 h. ( C ) TEM images of α-syn aggregated for 0 h, 24 h or 72 h in the presence or in the absence of G. frondosa or H. erinaceus 1×.

Article Snippet: Anti-α-Synuclein XP ® Rabbit (#51510 Cell Signaling Technology, Beverly, MA, USA) antibody was used at 1:1,000 dilution.

Techniques: Fluorescence

Journal: Nutrients

Article Title: Anti-Aging and Neuroprotective Properties of Grifola frondosa and Hericium erinaceus Extracts

doi: 10.3390/nu14204368

Figure Lengend Snippet: Fungal extract extends the lifespan of adult female and male flies. ( A ) Flies were supplemented with 0.05% G. frondosa extract lifelong. Data are presented as a percentage of survival of flies as a function of time (in days), evaluated in ten independent measurements for each group of flies by the Kaplan–Meier estimator (OASIS2 software). ( B ) The Log-rank test (OASIS2 software) was used to compare the mean lifespan of both male and female flies. *** p < 0.001 vs the respective CNT. ( C ) Western analysis of α-syn expression in fly brains supplemented or not with 0.05% G. frondosa extract. Representative immunoblot images and histograms of band-normalized OD are shown. Data are presented as mean ± SEM of three independent experiments.

Article Snippet: Anti-α-Synuclein XP ® Rabbit (#51510 Cell Signaling Technology, Beverly, MA, USA) antibody was used at 1:1,000 dilution.

Techniques: Software, Western Blot, Expressing