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(A) Western blots for ribosomal protein subunits <t>RPL5,</t> RPL7, RPL11, and RPL26 in WiT49 and 17.94 following 48-hour treatment of DMSO, 2nM actD, 8nM BTZ, or 2nM ActD + 8nM BTZ. (B) Protein levels of proteasome subunits PSMA6 (α1), PSMB1 (β6), PSMB2 (β4), PSMB5 (β5), and PSMD1 (P112), and POMP in WiT49 and 17.94 following 18-, 24-, 48-, and 72-hour 2nM actD treatment versus vehicle (DMSO). (C) Quantification of relative proteasome-specific chymotrypsin-like activity in WiT49 and 17.94 cells following 24 hours of DMSO versus 2nM actD, 8nM BTZ, or 2nM actD + 8nM BTZ treatments (Student’s t-test p-value versus vehicle: *<0.05, ** <0.01, ****<0.0001).
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(A) Western blots for ribosomal protein subunits <t>RPL5,</t> RPL7, RPL11, and RPL26 in WiT49 and 17.94 following 48-hour treatment of DMSO, 2nM actD, 8nM BTZ, or 2nM ActD + 8nM BTZ. (B) Protein levels of proteasome subunits PSMA6 (α1), PSMB1 (β6), PSMB2 (β4), PSMB5 (β5), and PSMD1 (P112), and POMP in WiT49 and 17.94 following 18-, 24-, 48-, and 72-hour 2nM actD treatment versus vehicle (DMSO). (C) Quantification of relative proteasome-specific chymotrypsin-like activity in WiT49 and 17.94 cells following 24 hours of DMSO versus 2nM actD, 8nM BTZ, or 2nM actD + 8nM BTZ treatments (Student’s t-test p-value versus vehicle: *<0.05, ** <0.01, ****<0.0001).
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(A) Western blots for ribosomal protein subunits <t>RPL5,</t> RPL7, RPL11, and RPL26 in WiT49 and 17.94 following 48-hour treatment of DMSO, 2nM actD, 8nM BTZ, or 2nM ActD + 8nM BTZ. (B) Protein levels of proteasome subunits PSMA6 (α1), PSMB1 (β6), PSMB2 (β4), PSMB5 (β5), and PSMD1 (P112), and POMP in WiT49 and 17.94 following 18-, 24-, 48-, and 72-hour 2nM actD treatment versus vehicle (DMSO). (C) Quantification of relative proteasome-specific chymotrypsin-like activity in WiT49 and 17.94 cells following 24 hours of DMSO versus 2nM actD, 8nM BTZ, or 2nM actD + 8nM BTZ treatments (Student’s t-test p-value versus vehicle: *<0.05, ** <0.01, ****<0.0001).
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Myxobacteria strains used. DSMZ refers to strains obtained from the German Collection of Microorganisms and Cell Cultures. Myxococcus llanfairPGensis is an abbreviation of Myxococcus llanfairpwllgwyngyllgogerychwyrndrobwllllantysiliogogogochensis .
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Myxobacteria strains used. DSMZ refers to strains obtained from the German Collection of Microorganisms and Cell Cultures. Myxococcus llanfairPGensis is an abbreviation of Myxococcus llanfairpwllgwyngyllgogerychwyrndrobwllllantysiliogogogochensis .
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Image Search Results


(A) Western blots for ribosomal protein subunits RPL5, RPL7, RPL11, and RPL26 in WiT49 and 17.94 following 48-hour treatment of DMSO, 2nM actD, 8nM BTZ, or 2nM ActD + 8nM BTZ. (B) Protein levels of proteasome subunits PSMA6 (α1), PSMB1 (β6), PSMB2 (β4), PSMB5 (β5), and PSMD1 (P112), and POMP in WiT49 and 17.94 following 18-, 24-, 48-, and 72-hour 2nM actD treatment versus vehicle (DMSO). (C) Quantification of relative proteasome-specific chymotrypsin-like activity in WiT49 and 17.94 cells following 24 hours of DMSO versus 2nM actD, 8nM BTZ, or 2nM actD + 8nM BTZ treatments (Student’s t-test p-value versus vehicle: *<0.05, ** <0.01, ****<0.0001).

Journal: bioRxiv

Article Title: Actinomycin D and bortezomib disrupt protein homeostasis in Wilms tumor

doi: 10.1101/2024.06.11.598518

Figure Lengend Snippet: (A) Western blots for ribosomal protein subunits RPL5, RPL7, RPL11, and RPL26 in WiT49 and 17.94 following 48-hour treatment of DMSO, 2nM actD, 8nM BTZ, or 2nM ActD + 8nM BTZ. (B) Protein levels of proteasome subunits PSMA6 (α1), PSMB1 (β6), PSMB2 (β4), PSMB5 (β5), and PSMD1 (P112), and POMP in WiT49 and 17.94 following 18-, 24-, 48-, and 72-hour 2nM actD treatment versus vehicle (DMSO). (C) Quantification of relative proteasome-specific chymotrypsin-like activity in WiT49 and 17.94 cells following 24 hours of DMSO versus 2nM actD, 8nM BTZ, or 2nM actD + 8nM BTZ treatments (Student’s t-test p-value versus vehicle: *<0.05, ** <0.01, ****<0.0001).

Article Snippet: Primary antibodies used are as follows and were diluted to 1:3,000 unless otherwise stated: Tubulin (Cell Signaling Technology 3873, RRID:AB_1904178), GAPDH (Cell Signaling Technology 97166, RRID:AB_2756824), Caspase-3 (Cell Signaling Technology 9665, RRID:AB_2069872), cleaved Caspase-3 (Cell Signaling Technology 9664, RRID:AB_2070042; 1:1,000), Caspase-7 (Cell Signaling Technology 12827, RRID:AB_2687912), cleaved Caspase-7 (Cell Signaling Technology 8438, RRID:AB_11178377; 1:1,000), Caspase-9 (Cell Signaling Technology 9508, RRID:AB_2068620), cleaved Caspase-9 (Cell Signaling Technology 7237, RRID:AB_10895832; 1:1,000), Cyclin D2 (Cell Signaling Technology 3741; RRID:AB_2070685), CDT1 (Cell Signaling Technology 8064, RRID:AB_10896851), Cyclin E1 Cell Signaling Technology 20808, RRID:AB_2783554), Cyclin A2 (Cell Signaling Technology 91500RRID:AB_3096041), PARP (Cell Signaling Technology 9532, RRID:AB_659884), cleaved PARP (Cell Signaling Technology 5625, RRID:AB_10699459; 1:1,000), POMP (Cell Signaling Technology 15141, RRID:AB_2798726), PSMA6 (Cell Signaling Technology 2459, RRID:AB_2268879), PSMB1 (Invitrogen PA5-49648, RRID: AB_2635102; 1:5,000), PSMB2 (Proteintech 15154-1-AP, RRID:AB_2300322; 1:5,000), PSMB5 (Cell Signaling Technology 12919, RRID:AB_2798061; 1:5,000), PSMD1 (Sigma SAB2104781, RRID:AB_10668741), RPL5 (Cell Signaling Techonology 51345, RRID:AB_2799391), RPL7 (abcam ab72550, RRID:AB_1270391), RPL11 (Cell Signaling Technology 18163, RRID:AB_2798794), RPL26 (Cell Signaling Technology 2065, RRID:AB_2146242), Total AKT (Cell Signaling Technology 9272, RRID:AB_329827), Phospho-AKT (Ser473) (Cell Signaling Technology 4060, RRID:AB_2315049; 1:1,000), Total 4E-BP1 (Cell Signaling Technology 9452, RRID:AB_331692), Phospho-4E-BP1 (Thr37/46) (Cell Signaling Technology 2855, RRID:AB_560835; 1:1,000), Total P70-S6K1 (Cell Signaling Technology 34475, RRID:AB_2943679), and Phospho-P70-S6K1 (Thr389) (Invitrogen 710095, AB_2532559; 1:1,000).

Techniques: Western Blot, Activity Assay

Myxobacteria strains used. DSMZ refers to strains obtained from the German Collection of Microorganisms and Cell Cultures. Myxococcus llanfairPGensis is an abbreviation of Myxococcus llanfairpwllgwyngyllgogerychwyrndrobwllllantysiliogogogochensis .

Journal: Microorganisms

Article Title: Myxobacterial Predation: A Standardised Lawn Predation Assay Highlights Strains with Unusually Efficient Predatory Activity

doi: 10.3390/microorganisms11020398

Figure Lengend Snippet: Myxobacteria strains used. DSMZ refers to strains obtained from the German Collection of Microorganisms and Cell Cultures. Myxococcus llanfairPGensis is an abbreviation of Myxococcus llanfairpwllgwyngyllgogerychwyrndrobwllllantysiliogogogochensis .

Article Snippet: Myxococcia , Corallococcus , Corallococcus coralloides DSM 2259 , DSMZ , GCA_000255295.

Techniques: