Journal: PLoS ONE

Article Title: Development of a Real-Time PCR for Identification of Brachyspira Species in Human Colonic Biopsies

doi: 10.1371/journal.pone.0052281

Figure Lengend Snippet: Primer sets, length of amplicon, annealing temperature, extension time and PCR-protocol.

Article Snippet: DNA from the type strains B. hyodysenteriae (B-78; ATCC 27164), B. innocens (B256; ATCC 29768), B. aalborgi (513A; NCTC 11492), B. pilosicoli (P43/6/78; ATCC 51139 and WesB), B. intermedia (PWS/A; ATCC 51140), B. murdochii (56–150; ATCC 51284) and B. alvinipulli (C1; ATCC 51933) (kindly donated by dr. D.J.

Techniques: Amplification, DNA Extraction, Sequencing

Journal: PLoS ONE

Article Title: Development of a Real-Time PCR for Identification of Brachyspira Species in Human Colonic Biopsies

doi: 10.1371/journal.pone.0052281

Figure Lengend Snippet: There is a clear difference in melting-temperature between the type-strain of B. pilosicoli and B. aalborgi . Biopsy samples containing these species fall neatly within these graphs (patients #13 and #23). “B. hominis” (patient #14) is visible as a peak between the type strains, indicating a different base-pair composition. The double infection (patient #26) begins as a B. pilosicoli -peak with a clear shoulder under the “B. hominis” peak, indicating that two different DNA molecules are present, each with a different melting-temperature-peak. The triple infection (patient #41) is displayed here by the biopsy sample that had both a B. pilosicoli and a B. aalborgi , another biopsy sample taken from this patient at the same time indicated an infection with “B. hominis” (data not shown).

Article Snippet: DNA from the type strains B. hyodysenteriae (B-78; ATCC 27164), B. innocens (B256; ATCC 29768), B. aalborgi (513A; NCTC 11492), B. pilosicoli (P43/6/78; ATCC 51139 and WesB), B. intermedia (PWS/A; ATCC 51140), B. murdochii (56–150; ATCC 51284) and B. alvinipulli (C1; ATCC 51933) (kindly donated by dr. D.J.

Techniques: Infection

Journal: PLoS ONE

Article Title: Development of a Real-Time PCR for Identification of Brachyspira Species in Human Colonic Biopsies

doi: 10.1371/journal.pone.0052281

Figure Lengend Snippet: Comparison of melting-curve prediction to sequence result * .

Article Snippet: DNA from the type strains B. hyodysenteriae (B-78; ATCC 27164), B. innocens (B256; ATCC 29768), B. aalborgi (513A; NCTC 11492), B. pilosicoli (P43/6/78; ATCC 51139 and WesB), B. intermedia (PWS/A; ATCC 51140), B. murdochii (56–150; ATCC 51284) and B. alvinipulli (C1; ATCC 51933) (kindly donated by dr. D.J.

Techniques: Sequencing

Journal: The Scientific World Journal

Article Title: Actinobaculum suis Detection Using Polymerase Chain Reaction

doi: 10.1100/2012/572732

Figure Lengend Snippet: Bacteria species used to test analytical specificity of PCR for Actinobaculum suis .

Article Snippet: Brachyspira pilosicoli , ATCC 51139.

Techniques:

Journal: Acta Veterinaria Scandinavica

Article Title: Neither Hippurate-negative Brachyspira pilosicoli nor Brachyspira pilosicoli Type Strain Caused Diarrhoea in Early-weaned Pigs by Experimental Infection

doi: 10.1186/1751-0147-46-257

Figure Lengend Snippet: Pigs inoculated with B. pilosicoli hipp- strain Br1622, B. pilosicoli type strain P43/6/78 or sterile broth (control). Pathology and results from fluorescent in situ hybridisation are shown.

Article Snippet: B. pilosicoli type strain P43/6/78 (ATCC 51139 T ) originated from a diarrhoeic pig in the UK [ ].

Techniques: In Situ, Hybridization

Journal: Acta Veterinaria Scandinavica

Article Title: Neither Hippurate-negative Brachyspira pilosicoli nor Brachyspira pilosicoli Type Strain Caused Diarrhoea in Early-weaned Pigs by Experimental Infection

doi: 10.1186/1751-0147-46-257

Figure Lengend Snippet: Invasion of spirochaetes in and below the colonic epithelium is shown in pig no. 13, which was inoculated with B. pilosicoli P43/6/78 and necropsied 16 days post-inoculation. Warthin-Starry silver staining. 1000×.

Article Snippet: B. pilosicoli type strain P43/6/78 (ATCC 51139 T ) originated from a diarrhoeic pig in the UK [ ].

Techniques: Silver Staining

Journal: PLoS ONE

Article Title: First Identification of “ Brachyspira hampsonii ” in Wild European Waterfowl

doi: 10.1371/journal.pone.0082626

Figure Lengend Snippet: The type and reference strains as well as the field isolates of Brachyspira spp. used for comparative purposes in the phylogenetic analysis.

Article Snippet: The B. hyodysenteriae B204 R (ATCC 31212) and B. pilosicoli P43/6/78 T (ATCC 51139) strains and nuclease-free water were employed as positive and negative controls, respectively.

Techniques:

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: 18 Brachyspira reference strains used for the development of the novel multiplex qPCR assay

Article Snippet: Given the genome size of 3.1 Mbp for B . hyodysenteriae ATCC 27164 (Mirajkar, Johnson, et al., ), 2.6 Mbp for B . pilosicoli ATCC 51139 (Lin et al., ), 3.3 Mbp for B . suanatina ATCC BAA2592 (Mushtaq et al., ), and 3.2 Mbp for B . hampsonii ATCC BAA2463 (Mirajkar, Phillips, et al., ) the following DNA quantities corresponded to 1 GE: 3.3 fg for B . hyodysenteriae , 2.8 fg for B . pilosicoli , 3.6 fg for B . suanatina ATCC BAA 2592, and 3.5 fg B . hampsonii .

Techniques: Multiplex Assay

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Amplification plots depicting five fluorophores used in the Brachyspira 5‐plex qPCR assay. Dilution series obtained from four reference strains ( B . hyodysenteriae ATCC 27164, B . suanatina ATCC BAA2592, B . hampsonii ATCC BAA2463, and B . pilosicoli ATCC 51139) in the linear range of 10 7 –100 genome equivalents representing each fluorophore individually. (a) Channel Green: probe 5’‐ FAM – MGB‐3’ detecting B . hyodysenteriae , (b) Channel Crimson: probe 5’‐ AlexaFluor680 – BHQ3 ‐3’ detecting B . suanatina , (c) Channel Orange: probe 5’‐ Rox – QXL610 ‐3’ detecting B . hampsonii , (d) Channel Yellow: probe 5’‐VIC – MGB ‐3’ detecting B . pilosicoli , (e) Channel Red: probe 5’‐ CY5 – BHQ1 ‐3’ detecting internal control eGFP

Article Snippet: Given the genome size of 3.1 Mbp for B . hyodysenteriae ATCC 27164 (Mirajkar, Johnson, et al., ), 2.6 Mbp for B . pilosicoli ATCC 51139 (Lin et al., ), 3.3 Mbp for B . suanatina ATCC BAA2592 (Mushtaq et al., ), and 3.2 Mbp for B . hampsonii ATCC BAA2463 (Mirajkar, Phillips, et al., ) the following DNA quantities corresponded to 1 GE: 3.3 fg for B . hyodysenteriae , 2.8 fg for B . pilosicoli , 3.6 fg for B . suanatina ATCC BAA 2592, and 3.5 fg B . hampsonii .

Techniques: Amplification

Journal: MicrobiologyOpen

Article Title: Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine

doi: 10.1002/mbo3.1169

Figure Lengend Snippet: Inter‐ and intra‐assay variability of reference strains B . hyodysenteriae , B . suanatina , B . pilosicoli , and B . hampsonii

Article Snippet: Given the genome size of 3.1 Mbp for B . hyodysenteriae ATCC 27164 (Mirajkar, Johnson, et al., ), 2.6 Mbp for B . pilosicoli ATCC 51139 (Lin et al., ), 3.3 Mbp for B . suanatina ATCC BAA2592 (Mushtaq et al., ), and 3.2 Mbp for B . hampsonii ATCC BAA2463 (Mirajkar, Phillips, et al., ) the following DNA quantities corresponded to 1 GE: 3.3 fg for B . hyodysenteriae , 2.8 fg for B . pilosicoli , 3.6 fg for B . suanatina ATCC BAA 2592, and 3.5 fg B . hampsonii .

Techniques: Intra Assay, Inter Assay