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virus strains sinorhizobium meliloti stain rm1021 atcc atcc51124 sinorhizobium meliloti strain rm1201 mcherry devers  (ATCC)


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    ATCC virus strains sinorhizobium meliloti stain rm1021 atcc atcc51124 sinorhizobium meliloti strain rm1201 mcherry devers
    Virus Strains Sinorhizobium Meliloti Stain Rm1021 Atcc Atcc51124 Sinorhizobium Meliloti Strain Rm1201 Mcherry Devers, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC virus strains sinorhizobium meliloti stain rm1021 atcc atcc51124 sinorhizobium meliloti strain rm1201 mcherry devers
    Virus Strains Sinorhizobium Meliloti Stain Rm1021 Atcc Atcc51124 Sinorhizobium Meliloti Strain Rm1201 Mcherry Devers, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc tph2
    List of primers used for quantitating mRNA expression using RT-qPCR
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    ( A ) <t>Tph2</t> expression levels in the brain stem (BS) of 3-month-old TgSirt1 mice ( n = 5) versus WT controls ( n = 5). ( B ) 5HT levels in brain stem of TgSirt1 mice ( n = 6) versus WT controls ( n = 4) measured by HPLC. ( C ) MAO-A expression levels in the rest of brain (ROB) of 3-month-old TgSirt1 mice ( n = 4) versus WT controls ( n = 4). ( D ) MAO-A activity (10 3 RLU/μg protein/h) in the hypothalamus, brain stem, and rest of brain of TgSirt1 mice treated with phenelzine ( n = 5) versus vehicle controls ( n = 5). ( E ) 5HT and 5-HIAA levels in brain stem and rest of brain of TgSirt1 mice treated with phenelzine ( n = 5) versus vehicle controls ( n = 5) measured by HPLC. ( F ) BV/TV (%); ( G ) N.Ob/T.Ar (/mm 2 ); ( H ) BFR/BS (μm 3 /μm 2 /yr); and ( I ) Oc.S/BS (%) of 3-month-old TgSirt1 mice treated with phenelzine ( n = 5) versus vehicle ( n = 6) and WT controls ( n = 5). ( J ) Representative images of spines from 3-month-old TgSirt1 mice treated with phenelzine versus vehicle and WT controls stained with von Kossa. ( K ) Dbh expression levels in midbrain (MB) of 3-month-old TgSirt1 mice ( n = 5) versus WT controls ( n = 5). ( L ) Tph2 expression levels in brain stem of 3-month-old Sirt1 brain –/– mice ( n = 4) versus Sirt1 COIN/COIN controls ( n = 4). ( M ) MAO-A expression levels in rest of brain of 3-month-old Sirt1 brain –/– mice ( n = 4) versus Sirt1 COIN/COIN controls ( n = 4). ( N ) Dbh expression levels in MB of 3-month-old Sirt1 brain –/– mice ( n = 4) versus Sirt1 COIN/COIN controls ( n = 4). Data are represented as mean ± SEM. ( A – E and K – N ) *P < 0.05, Student’s t test. ( F – I ) *P < 0.05, TgSirt1 mice treated with phenelzine versus vehicle by 1-way ANOVA.
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    Cell Signaling Technology Inc human tph2
    Determination of hepatocyte markers and function after differentiation in vitro . ((a) and (b)) Determination of hepatocyte markers by western blotting. (c) Typical high-performance liquid chromatography/mass spectrometry chromatograms of midazolam and diazepam (internal standard) in culture media samples. (1) Blank culture medium + diazepam; (2) blank culture medium + midazolam (500 ng/mL) + diazepam; (3) sample culture medium after 2 h incubation with hUCMSCs induced by LHS. (d) Linear curve for midazolam. (e) Effects of LHS on CYP3A enzyme activity in hUCMSCs after 3, 5, and 7 days. (f) Effects of LHS on blood urea nitrogen production in hUCMSCs after 3, 5, and 7 days. (g) Effects of LHS on ALB externalization in hUCMSCs after 3, 5, and 7 days (mean ± SD, n = 6, ∗ P < 0.01 compared with 0 d, # P < 0.05 compared with 3 d, and △ P < 0.05 compared with 5 d). hUCMSC, human umbilical cord-derived mesenchymal stem cell; NC, negative control group; PC, positive control group of QSG-7701 human hepatic cell line; 0 d, undifferentiated hUCMSCs; 3 d, 5 d, 7 d, 3 d, 5 d, and 7 days after induction by LSH, respectively; AFP, α -fetoprotein; CK18, cytokeratin 18; <t>TPH2,</t> tryptophan 2,3-dioxygenase; LHS, liver homogenate supernatant.
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    Determination of hepatocyte markers and function after differentiation in vitro . ((a) and (b)) Determination of hepatocyte markers by western blotting. (c) Typical high-performance liquid chromatography/mass spectrometry chromatograms of midazolam and diazepam (internal standard) in culture media samples. (1) Blank culture medium + diazepam; (2) blank culture medium + midazolam (500 ng/mL) + diazepam; (3) sample culture medium after 2 h incubation with hUCMSCs induced by LHS. (d) Linear curve for midazolam. (e) Effects of LHS on CYP3A enzyme activity in hUCMSCs after 3, 5, and 7 days. (f) Effects of LHS on blood urea nitrogen production in hUCMSCs after 3, 5, and 7 days. (g) Effects of LHS on ALB externalization in hUCMSCs after 3, 5, and 7 days (mean ± SD, n = 6, ∗ P < 0.01 compared with 0 d, # P < 0.05 compared with 3 d, and △ P < 0.05 compared with 5 d). hUCMSC, human umbilical cord-derived mesenchymal stem cell; NC, negative control group; PC, positive control group of QSG-7701 human hepatic cell line; 0 d, undifferentiated hUCMSCs; 3 d, 5 d, 7 d, 3 d, 5 d, and 7 days after induction by LSH, respectively; AFP, α -fetoprotein; CK18, cytokeratin 18; <t>TPH2,</t> tryptophan 2,3-dioxygenase; LHS, liver homogenate supernatant.
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    Determination of hepatocyte markers and function after differentiation in vitro . ((a) and (b)) Determination of hepatocyte markers by western blotting. (c) Typical high-performance liquid chromatography/mass spectrometry chromatograms of midazolam and diazepam (internal standard) in culture media samples. (1) Blank culture medium + diazepam; (2) blank culture medium + midazolam (500 ng/mL) + diazepam; (3) sample culture medium after 2 h incubation with hUCMSCs induced by LHS. (d) Linear curve for midazolam. (e) Effects of LHS on CYP3A enzyme activity in hUCMSCs after 3, 5, and 7 days. (f) Effects of LHS on blood urea nitrogen production in hUCMSCs after 3, 5, and 7 days. (g) Effects of LHS on ALB externalization in hUCMSCs after 3, 5, and 7 days (mean ± SD, n = 6, ∗ P < 0.01 compared with 0 d, # P < 0.05 compared with 3 d, and △ P < 0.05 compared with 5 d). hUCMSC, human umbilical cord-derived mesenchymal stem cell; NC, negative control group; PC, positive control group of QSG-7701 human hepatic cell line; 0 d, undifferentiated hUCMSCs; 3 d, 5 d, 7 d, 3 d, 5 d, and 7 days after induction by LSH, respectively; AFP, α -fetoprotein; CK18, cytokeratin 18; <t>TPH2,</t> tryptophan 2,3-dioxygenase; LHS, liver homogenate supernatant.
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    Image Search Results


    List of primers used for quantitating mRNA expression using RT-qPCR

    Journal: Acta Neuropathologica Communications

    Article Title: Human tau-overexpressing mice recapitulate brainstem involvement and neuropsychiatric features of early Alzheimer’s disease

    doi: 10.1186/s40478-023-01546-5

    Figure Lengend Snippet: List of primers used for quantitating mRNA expression using RT-qPCR

    Article Snippet: TPH2 , Primary , 51124 , Cell signaling technology , 1:2000.

    Techniques: Expressing, Sequencing, Transmission Assay

    Antibodies used in Western blot

    Journal: Acta Neuropathologica Communications

    Article Title: Human tau-overexpressing mice recapitulate brainstem involvement and neuropsychiatric features of early Alzheimer’s disease

    doi: 10.1186/s40478-023-01546-5

    Figure Lengend Snippet: Antibodies used in Western blot

    Article Snippet: TPH2 , Primary , 51124 , Cell signaling technology , 1:2000.

    Techniques: Western Blot

    Reduction in 5-HT neuronal excitability in htau mice at 4 months. A Confocal images of biocytin-labeled cells (red) in the DRN overlaid with TPH2 staining (green) indicating that recorded cells were 5-HT neurons. B Schematic of patch clamp recordings in the DRN and representative traces of voltage ramps that were used to compute the rheobase and action potential (AP) firing at the 200 pA current step from resting membrane potential (RMP). Intracellular recordings in 5-HT neurons from 4-month-old male C57BL/6 J and htau +/- mice with histograms indicating C RMP, D Input resistance at RMP, E Rheobase and F Current (0–200 pA)-induced spiking in 250 ms from RMP G Representative voltage ramps and AP-current plot at 200 pA current step from a holding potential of − 70 mV. H Input resistance I Rheobase and J Current induced spiking (0–200 pA) from a holding potential of − 70 mV. Action potential kinetics showing the K 10–90% rise slope, L Maximum decay slope, M Time to maximum decay slope, N Event Frequency, and O Instantaneous frequency at 0–200 pA current steps from the RMP. * p < 0.05

    Journal: Acta Neuropathologica Communications

    Article Title: Human tau-overexpressing mice recapitulate brainstem involvement and neuropsychiatric features of early Alzheimer’s disease

    doi: 10.1186/s40478-023-01546-5

    Figure Lengend Snippet: Reduction in 5-HT neuronal excitability in htau mice at 4 months. A Confocal images of biocytin-labeled cells (red) in the DRN overlaid with TPH2 staining (green) indicating that recorded cells were 5-HT neurons. B Schematic of patch clamp recordings in the DRN and representative traces of voltage ramps that were used to compute the rheobase and action potential (AP) firing at the 200 pA current step from resting membrane potential (RMP). Intracellular recordings in 5-HT neurons from 4-month-old male C57BL/6 J and htau +/- mice with histograms indicating C RMP, D Input resistance at RMP, E Rheobase and F Current (0–200 pA)-induced spiking in 250 ms from RMP G Representative voltage ramps and AP-current plot at 200 pA current step from a holding potential of − 70 mV. H Input resistance I Rheobase and J Current induced spiking (0–200 pA) from a holding potential of − 70 mV. Action potential kinetics showing the K 10–90% rise slope, L Maximum decay slope, M Time to maximum decay slope, N Event Frequency, and O Instantaneous frequency at 0–200 pA current steps from the RMP. * p < 0.05

    Article Snippet: TPH2 , Primary , 51124 , Cell signaling technology , 1:2000.

    Techniques: Labeling, Staining, Patch Clamp

    Hyperphosphorylated tau and altered expression of TPH2, TH, IDO1, and TGM2 protein levels in the monoaminergic nuclei. A Atlas plate indicating the DRN region that was dissected for Western blot analysis. Representative western blot images showing the B HT7 and AH36 C TPH2 D IDO1 E TGM2 protein levels in the DRN of C57BL/6 J and htau +/- . F Atlas plate indicating the LC region that was dissected for Western blot analysis. Representative western blot images showing the G HT7 and AH36 H TH I IDO1 J TGM2 protein levels in the LC of C57BL/6 J and htau ± . * p < 0.05, ** p < 0.01

    Journal: Acta Neuropathologica Communications

    Article Title: Human tau-overexpressing mice recapitulate brainstem involvement and neuropsychiatric features of early Alzheimer’s disease

    doi: 10.1186/s40478-023-01546-5

    Figure Lengend Snippet: Hyperphosphorylated tau and altered expression of TPH2, TH, IDO1, and TGM2 protein levels in the monoaminergic nuclei. A Atlas plate indicating the DRN region that was dissected for Western blot analysis. Representative western blot images showing the B HT7 and AH36 C TPH2 D IDO1 E TGM2 protein levels in the DRN of C57BL/6 J and htau +/- . F Atlas plate indicating the LC region that was dissected for Western blot analysis. Representative western blot images showing the G HT7 and AH36 H TH I IDO1 J TGM2 protein levels in the LC of C57BL/6 J and htau ± . * p < 0.05, ** p < 0.01

    Article Snippet: TPH2 , Primary , 51124 , Cell signaling technology , 1:2000.

    Techniques: Expressing, Western Blot

    ( A ) Tph2 expression levels in the brain stem (BS) of 3-month-old TgSirt1 mice ( n = 5) versus WT controls ( n = 5). ( B ) 5HT levels in brain stem of TgSirt1 mice ( n = 6) versus WT controls ( n = 4) measured by HPLC. ( C ) MAO-A expression levels in the rest of brain (ROB) of 3-month-old TgSirt1 mice ( n = 4) versus WT controls ( n = 4). ( D ) MAO-A activity (10 3 RLU/μg protein/h) in the hypothalamus, brain stem, and rest of brain of TgSirt1 mice treated with phenelzine ( n = 5) versus vehicle controls ( n = 5). ( E ) 5HT and 5-HIAA levels in brain stem and rest of brain of TgSirt1 mice treated with phenelzine ( n = 5) versus vehicle controls ( n = 5) measured by HPLC. ( F ) BV/TV (%); ( G ) N.Ob/T.Ar (/mm 2 ); ( H ) BFR/BS (μm 3 /μm 2 /yr); and ( I ) Oc.S/BS (%) of 3-month-old TgSirt1 mice treated with phenelzine ( n = 5) versus vehicle ( n = 6) and WT controls ( n = 5). ( J ) Representative images of spines from 3-month-old TgSirt1 mice treated with phenelzine versus vehicle and WT controls stained with von Kossa. ( K ) Dbh expression levels in midbrain (MB) of 3-month-old TgSirt1 mice ( n = 5) versus WT controls ( n = 5). ( L ) Tph2 expression levels in brain stem of 3-month-old Sirt1 brain –/– mice ( n = 4) versus Sirt1 COIN/COIN controls ( n = 4). ( M ) MAO-A expression levels in rest of brain of 3-month-old Sirt1 brain –/– mice ( n = 4) versus Sirt1 COIN/COIN controls ( n = 4). ( N ) Dbh expression levels in MB of 3-month-old Sirt1 brain –/– mice ( n = 4) versus Sirt1 COIN/COIN controls ( n = 4). Data are represented as mean ± SEM. ( A – E and K – N ) *P < 0.05, Student’s t test. ( F – I ) *P < 0.05, TgSirt1 mice treated with phenelzine versus vehicle by 1-way ANOVA.

    Journal: The Journal of Clinical Investigation

    Article Title: A neuronal action of sirtuin 1 suppresses bone mass in young and aging mice

    doi: 10.1172/JCI152868

    Figure Lengend Snippet: ( A ) Tph2 expression levels in the brain stem (BS) of 3-month-old TgSirt1 mice ( n = 5) versus WT controls ( n = 5). ( B ) 5HT levels in brain stem of TgSirt1 mice ( n = 6) versus WT controls ( n = 4) measured by HPLC. ( C ) MAO-A expression levels in the rest of brain (ROB) of 3-month-old TgSirt1 mice ( n = 4) versus WT controls ( n = 4). ( D ) MAO-A activity (10 3 RLU/μg protein/h) in the hypothalamus, brain stem, and rest of brain of TgSirt1 mice treated with phenelzine ( n = 5) versus vehicle controls ( n = 5). ( E ) 5HT and 5-HIAA levels in brain stem and rest of brain of TgSirt1 mice treated with phenelzine ( n = 5) versus vehicle controls ( n = 5) measured by HPLC. ( F ) BV/TV (%); ( G ) N.Ob/T.Ar (/mm 2 ); ( H ) BFR/BS (μm 3 /μm 2 /yr); and ( I ) Oc.S/BS (%) of 3-month-old TgSirt1 mice treated with phenelzine ( n = 5) versus vehicle ( n = 6) and WT controls ( n = 5). ( J ) Representative images of spines from 3-month-old TgSirt1 mice treated with phenelzine versus vehicle and WT controls stained with von Kossa. ( K ) Dbh expression levels in midbrain (MB) of 3-month-old TgSirt1 mice ( n = 5) versus WT controls ( n = 5). ( L ) Tph2 expression levels in brain stem of 3-month-old Sirt1 brain –/– mice ( n = 4) versus Sirt1 COIN/COIN controls ( n = 4). ( M ) MAO-A expression levels in rest of brain of 3-month-old Sirt1 brain –/– mice ( n = 4) versus Sirt1 COIN/COIN controls ( n = 4). ( N ) Dbh expression levels in MB of 3-month-old Sirt1 brain –/– mice ( n = 4) versus Sirt1 COIN/COIN controls ( n = 4). Data are represented as mean ± SEM. ( A – E and K – N ) *P < 0.05, Student’s t test. ( F – I ) *P < 0.05, TgSirt1 mice treated with phenelzine versus vehicle by 1-way ANOVA.

    Article Snippet: Antibodies used were as follows: anti-GFP mouse monoclonal antibody (Takara, catalog 632375); anti-Sirt1 rabbit antibody (Cell Signaling Technology, catalog 2028S); anti-Tph2 rabbit polyclonal antibody (Novus NB, catalog 100-74555); Alexa Fluor 488 AffiniPure donkey anti-mouse IgG (Jackson ImmunoResearch, catalog 715-545-150); and Alexa Fluor 594 AffiniPure donkey, anti-rabbit IgG (Jackson ImmunoResearch, catalog 711-585-152).

    Techniques: Expressing, Activity Assay, Staining

    ( A ) Ucp1 expression in BAT of Sirt1 Syn –/– mice ( n = 7) versus controls ( n = 7). ( B ) Expression of SNS target genes in long bone of Sirt1 Syn –/– mice ( n = 5) versus controls ( n = 5). ( C ) Tph2 expression in brain stem of Sirt1 Syn –/– mice ( n = 7) versus controls ( n = 7). ( D ) MAO-A expression and ( E ) MAO-A activity in rest of brain of Sirt1 Syn –/– mice ( n = 5) versus controls ( n = 5). ( F ) Dbh expression in MB of Sirt1 Syn –/– mice ( n = 5) versus controls ( n = 5). ( G ) Bche expression in hypothalamus of Sirt1 Syn –/– mice ( n = 6) versus controls ( n = 6). ( H ) Ucp1 expression in BAT of Sirt1 Sert –/– mice ( n = 4) versus controls ( n = 8). ( I ) Expression of SNS target genes in long bone of Sirt1 Sert –/– mice ( n = 4) versus controls ( n = 4). ( J ) Tph2 expression in brain stem of Sirt1 Sert –/– mice ( n = 4) versus controls ( n = 6). ( K ) MAO-A expression in rest of brain of Sirt1 Sert –/– mice ( n = 4) versus controls ( n = 8). ( L ) MAO-A activity in rest of brain of Sirt1 Sert –/– mice ( n = 5) versus controls ( n = 5). ( M ) Dbh expression in MB of Sirt1 Sert –/– mice ( n = 4) versus controls ( n = 5). ( N ) Bche expression in hypothalamus of Sirt1 Sert –/– mice( n = 4) versus controls ( n = 4). ( O ) Ucp1 expression in BAT of Sirt1 Dbh –/– mice ( n = 5) versus controls ( n = 5). ( P ) Expression of SNS target genes in long bone of Sirt1 Dbh –/– mice ( n = 5) versus controls ( n = 5). ( Q ) Tph2 expression in brain stem of Sirt1 Dbh –/– mice ( n = 4) versus controls ( n = 5). ( R ) MAO-A expression in rest of brain of Sirt1 Dbh –/– mice ( n = 4) versus controls ( n = 5). ( S ) MAO-A activity in rest of brain of Sirt1 Dbh –/– mice ( n = 5) versus controls ( n = 5). ( T ) Dbh expression in MB and ( U ) Bche expression in hypothalamus of Sirt1 Dbh –/– ( n = 4) versus controls ( n = 5). Data are represented as mean ± SEM. * P < 0.05 versus Sirt1 COIN/COIN by Student’s t test.

    Journal: The Journal of Clinical Investigation

    Article Title: A neuronal action of sirtuin 1 suppresses bone mass in young and aging mice

    doi: 10.1172/JCI152868

    Figure Lengend Snippet: ( A ) Ucp1 expression in BAT of Sirt1 Syn –/– mice ( n = 7) versus controls ( n = 7). ( B ) Expression of SNS target genes in long bone of Sirt1 Syn –/– mice ( n = 5) versus controls ( n = 5). ( C ) Tph2 expression in brain stem of Sirt1 Syn –/– mice ( n = 7) versus controls ( n = 7). ( D ) MAO-A expression and ( E ) MAO-A activity in rest of brain of Sirt1 Syn –/– mice ( n = 5) versus controls ( n = 5). ( F ) Dbh expression in MB of Sirt1 Syn –/– mice ( n = 5) versus controls ( n = 5). ( G ) Bche expression in hypothalamus of Sirt1 Syn –/– mice ( n = 6) versus controls ( n = 6). ( H ) Ucp1 expression in BAT of Sirt1 Sert –/– mice ( n = 4) versus controls ( n = 8). ( I ) Expression of SNS target genes in long bone of Sirt1 Sert –/– mice ( n = 4) versus controls ( n = 4). ( J ) Tph2 expression in brain stem of Sirt1 Sert –/– mice ( n = 4) versus controls ( n = 6). ( K ) MAO-A expression in rest of brain of Sirt1 Sert –/– mice ( n = 4) versus controls ( n = 8). ( L ) MAO-A activity in rest of brain of Sirt1 Sert –/– mice ( n = 5) versus controls ( n = 5). ( M ) Dbh expression in MB of Sirt1 Sert –/– mice ( n = 4) versus controls ( n = 5). ( N ) Bche expression in hypothalamus of Sirt1 Sert –/– mice( n = 4) versus controls ( n = 4). ( O ) Ucp1 expression in BAT of Sirt1 Dbh –/– mice ( n = 5) versus controls ( n = 5). ( P ) Expression of SNS target genes in long bone of Sirt1 Dbh –/– mice ( n = 5) versus controls ( n = 5). ( Q ) Tph2 expression in brain stem of Sirt1 Dbh –/– mice ( n = 4) versus controls ( n = 5). ( R ) MAO-A expression in rest of brain of Sirt1 Dbh –/– mice ( n = 4) versus controls ( n = 5). ( S ) MAO-A activity in rest of brain of Sirt1 Dbh –/– mice ( n = 5) versus controls ( n = 5). ( T ) Dbh expression in MB and ( U ) Bche expression in hypothalamus of Sirt1 Dbh –/– ( n = 4) versus controls ( n = 5). Data are represented as mean ± SEM. * P < 0.05 versus Sirt1 COIN/COIN by Student’s t test.

    Article Snippet: Antibodies used were as follows: anti-GFP mouse monoclonal antibody (Takara, catalog 632375); anti-Sirt1 rabbit antibody (Cell Signaling Technology, catalog 2028S); anti-Tph2 rabbit polyclonal antibody (Novus NB, catalog 100-74555); Alexa Fluor 488 AffiniPure donkey anti-mouse IgG (Jackson ImmunoResearch, catalog 715-545-150); and Alexa Fluor 594 AffiniPure donkey, anti-rabbit IgG (Jackson ImmunoResearch, catalog 711-585-152).

    Techniques: Expressing, Activity Assay

    Strains used in this study.

    Journal: PLoS ONE

    Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

    doi: 10.1371/journal.pone.0108877

    Figure Lengend Snippet: Strains used in this study.

    Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

    Techniques:

    A lane, 1, Archangium gephyra DSM 2261; lane 2, Corallococcus macrospores DSM 14697; lane 3, Corallococcus coralloides DSM 52499; lane 4, Myxococcus fulvus DSM 16525; lane 5, Myxococcus virescens DSM 2260; lane 6, Cystobacter minus DSM 14751; lane 7, Stigmatella erecta DSM 16858; lane 8, Byssovorax cruenta DSM 14533; B, Bands (1∼28) that were excised for sequence analysis are numbered.

    Journal: PLoS ONE

    Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

    doi: 10.1371/journal.pone.0108877

    Figure Lengend Snippet: A lane, 1, Archangium gephyra DSM 2261; lane 2, Corallococcus macrospores DSM 14697; lane 3, Corallococcus coralloides DSM 52499; lane 4, Myxococcus fulvus DSM 16525; lane 5, Myxococcus virescens DSM 2260; lane 6, Cystobacter minus DSM 14751; lane 7, Stigmatella erecta DSM 16858; lane 8, Byssovorax cruenta DSM 14533; B, Bands (1∼28) that were excised for sequence analysis are numbered.

    Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

    Techniques: Sequencing

    Strains used in this study.

    Journal: PLoS ONE

    Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

    doi: 10.1371/journal.pone.0108877

    Figure Lengend Snippet: Strains used in this study.

    Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

    Techniques:

    A lane, 1, Archangium gephyra DSM 2261; lane 2, Corallococcus macrospores DSM 14697; lane 3, Corallococcus coralloides DSM 52499; lane 4, Myxococcus fulvus DSM 16525; lane 5, Myxococcus virescens DSM 2260; lane 6, Cystobacter minus DSM 14751; lane 7, Stigmatella erecta DSM 16858; lane 8, Byssovorax cruenta DSM 14533; B, Bands (1∼28) that were excised for sequence analysis are numbered.

    Journal: PLoS ONE

    Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

    doi: 10.1371/journal.pone.0108877

    Figure Lengend Snippet: A lane, 1, Archangium gephyra DSM 2261; lane 2, Corallococcus macrospores DSM 14697; lane 3, Corallococcus coralloides DSM 52499; lane 4, Myxococcus fulvus DSM 16525; lane 5, Myxococcus virescens DSM 2260; lane 6, Cystobacter minus DSM 14751; lane 7, Stigmatella erecta DSM 16858; lane 8, Byssovorax cruenta DSM 14533; B, Bands (1∼28) that were excised for sequence analysis are numbered.

    Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

    Techniques: Sequencing

    Sequence similarity of excised DNA fragments.

    Journal: PLoS ONE

    Article Title: Approach to Analyze the Diversity of Myxobacteria in Soil by Semi-Nested PCR-Denaturing Gradient Gel Electrophoresis (DGGE) Based on Taxon-Specific Gene

    doi: 10.1371/journal.pone.0108877

    Figure Lengend Snippet: Sequence similarity of excised DNA fragments.

    Article Snippet: Corallococcus coralloides , DSM 52499 , Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales , DSMZ Germany.

    Techniques: Sequencing

    Determination of hepatocyte markers and function after differentiation in vitro . ((a) and (b)) Determination of hepatocyte markers by western blotting. (c) Typical high-performance liquid chromatography/mass spectrometry chromatograms of midazolam and diazepam (internal standard) in culture media samples. (1) Blank culture medium + diazepam; (2) blank culture medium + midazolam (500 ng/mL) + diazepam; (3) sample culture medium after 2 h incubation with hUCMSCs induced by LHS. (d) Linear curve for midazolam. (e) Effects of LHS on CYP3A enzyme activity in hUCMSCs after 3, 5, and 7 days. (f) Effects of LHS on blood urea nitrogen production in hUCMSCs after 3, 5, and 7 days. (g) Effects of LHS on ALB externalization in hUCMSCs after 3, 5, and 7 days (mean ± SD, n = 6, ∗ P < 0.01 compared with 0 d, # P < 0.05 compared with 3 d, and △ P < 0.05 compared with 5 d). hUCMSC, human umbilical cord-derived mesenchymal stem cell; NC, negative control group; PC, positive control group of QSG-7701 human hepatic cell line; 0 d, undifferentiated hUCMSCs; 3 d, 5 d, 7 d, 3 d, 5 d, and 7 days after induction by LSH, respectively; AFP, α -fetoprotein; CK18, cytokeratin 18; TPH2, tryptophan 2,3-dioxygenase; LHS, liver homogenate supernatant.

    Journal: BioMed Research International

    Article Title: Effect of Microenvironment on Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Hepatocytes In Vitro and In Vivo

    doi: 10.1155/2016/8916534

    Figure Lengend Snippet: Determination of hepatocyte markers and function after differentiation in vitro . ((a) and (b)) Determination of hepatocyte markers by western blotting. (c) Typical high-performance liquid chromatography/mass spectrometry chromatograms of midazolam and diazepam (internal standard) in culture media samples. (1) Blank culture medium + diazepam; (2) blank culture medium + midazolam (500 ng/mL) + diazepam; (3) sample culture medium after 2 h incubation with hUCMSCs induced by LHS. (d) Linear curve for midazolam. (e) Effects of LHS on CYP3A enzyme activity in hUCMSCs after 3, 5, and 7 days. (f) Effects of LHS on blood urea nitrogen production in hUCMSCs after 3, 5, and 7 days. (g) Effects of LHS on ALB externalization in hUCMSCs after 3, 5, and 7 days (mean ± SD, n = 6, ∗ P < 0.01 compared with 0 d, # P < 0.05 compared with 3 d, and △ P < 0.05 compared with 5 d). hUCMSC, human umbilical cord-derived mesenchymal stem cell; NC, negative control group; PC, positive control group of QSG-7701 human hepatic cell line; 0 d, undifferentiated hUCMSCs; 3 d, 5 d, 7 d, 3 d, 5 d, and 7 days after induction by LSH, respectively; AFP, α -fetoprotein; CK18, cytokeratin 18; TPH2, tryptophan 2,3-dioxygenase; LHS, liver homogenate supernatant.

    Article Snippet: Liver slides were then incubated with diluted primary antibodies against anti-human CK18 (1 : 100, Abcam, Cambridge Science Park, Cambridge, UK), human AFP (1 : 50), human TPH2 (1 : 20), and human ALB (1 : 50) (Cell Signaling Technology, Inc., Danvers, MA, USA), followed by biotin-conjugated secondary antibody.

    Techniques: In Vitro, Western Blot, High Performance Liquid Chromatography, Mass Spectrometry, Incubation, Activity Assay, Derivative Assay, Negative Control, Positive Control

    Immunohistochemical determination of human hepatocyte markers in rat liver after transplantation of hUCMSCs. (a) Expression of CK18, ALB, AFP, and TPH2 in liver after transplantation of hUCMSCs into acute CCl 4 -treated rats. (b) Quantification of human hepatocyte-specific marker positive cells (mean ± SD, n = 6, ∗ P < 0.01 compared with vehicle group, # P < 0.05 compared with 3 h group, and △ P < 0.05 compared with 2 d group). Bar = 100 μ m.

    Journal: BioMed Research International

    Article Title: Effect of Microenvironment on Differentiation of Human Umbilical Cord Mesenchymal Stem Cells into Hepatocytes In Vitro and In Vivo

    doi: 10.1155/2016/8916534

    Figure Lengend Snippet: Immunohistochemical determination of human hepatocyte markers in rat liver after transplantation of hUCMSCs. (a) Expression of CK18, ALB, AFP, and TPH2 in liver after transplantation of hUCMSCs into acute CCl 4 -treated rats. (b) Quantification of human hepatocyte-specific marker positive cells (mean ± SD, n = 6, ∗ P < 0.01 compared with vehicle group, # P < 0.05 compared with 3 h group, and △ P < 0.05 compared with 2 d group). Bar = 100 μ m.

    Article Snippet: Liver slides were then incubated with diluted primary antibodies against anti-human CK18 (1 : 100, Abcam, Cambridge Science Park, Cambridge, UK), human AFP (1 : 50), human TPH2 (1 : 20), and human ALB (1 : 50) (Cell Signaling Technology, Inc., Danvers, MA, USA), followed by biotin-conjugated secondary antibody.

    Techniques: Immunohistochemical staining, Transplantation Assay, Expressing, Marker